The Action of Various Agents upon the Rabbit Embryo

Development ◽  
1961 ◽  
Vol 9 (3) ◽  
pp. 468-491
Author(s):  
C. E. Adams ◽  
Mary F. Hay ◽  
Cecilia Lutwak-Mann
Keyword(s):  
Histological Examination ◽  
Severe Damage ◽  
Purine Analogues ◽  
Embryonic Disc ◽  
Rabbit Embryo

In the course of an investigation on the uptake of labelled ions by the rabbit embryo and its environment (Lutwak-Mann, Boursnell, & Bennett, 1960), experiments were done on pre-implantation blastocysts obtained from rabbits which had been treated parenterally with certain purine analogues. Histological examination of such blastocysts by the method of Moog & Lutwak-Mann (1958) showed that, as a result of treatment of the pregnant animals, the embryos had incurred severe damage chiefly localized in the embryonic disc. This observation prompted a wider study, reported below, of the action of various agents upon the early rabbit embryo, following their administration to the mother. Our investigation was chiefly concerned with the pre-implantation 6½-day-old blastocyst, but 5- and 7-day embryos were also examined. At the same time we have studied the influence of some of these agents upon ovulation, fertilization, and cleavage, as well as on implantation and later stages of pregnancy.

Low proliferative and high migratory activity in the area of Brachyury expressing mesoderm progenitor cells in the gastrulating rabbit embryo

Development ◽  
2002 ◽  
Vol 129 (10) ◽  
pp. 2355-2365 ◽  
Author(s):  
Christoph Viebahn ◽  
Christof Stortz ◽  
Sally A. Mitchell ◽  
Martin Blum
Keyword(s):  
Anterior Part ◽  
In Situ Hybridisation ◽  
Primitive Streak ◽  
Cellular Migration ◽  
Avian Embryo ◽  
Migratory Activity ◽  
Interspecific Differences ◽  
Embryonic Disc ◽  
Rabbit Embryo

General mechanisms initiating the gastrulation process in early animal development are still elusive, not least because embryonic morphology differs widely among species. The rabbit embryo is revived here as a model to study vertebrate gastrulation, because its relatively simple morphology at the appropriate stages makes interspecific differences and similarities particularly obvious between mammals and birds. Three approaches that centre on mesoderm specification as a key event at the start of gastrulation were chosen. (1) A cDNA fragment encoding 212 amino acids of the rabbit Brachyury gene was cloned by RT-PCR and used as a molecular marker for mesoderm progenitors. Whole-mount in situ hybridisation revealed single Brachyury-expressing cells in the epiblast at 6.2 days post conception, i.e. several hours before the first ingressing mesoderm cells can be detected histologically. With the anterior marginal crescent as a landmark, these mesoderm progenitors are shown to lie in a posterior quadrant of the embryonic disc, which we call the posterior gastrula extension (PGE), for reasons established during the following functional analysis. (2) Vital dye (DiI) labelling in vitro suggests that epiblast cells arrive in the PGE from anterior parts of the embryonic disc and then move within this area in a complex pattern of posterior, centripetal and anterior directions to form the primitive streak. (3) BrdU labelling shows that proliferation is reduced in the PGE, while the remaining anterior part of the embryonic disc contains several areas of increased proliferation. These results reveal similarities with the chick with respect to Brachyury expression and cellular migration. They differ, however, in that local differences in proliferation are not seen in the pre-streak avian embryo. Rather, rabbit epiblast cells start mesoderm differentiation in a way similar to Drosophila, where a transient downregulation of proliferation initiates mesoderm differentiation and, hence, gastrulation.

Effects of feeding formalin-treated skim milk to calves

1960 ◽  
Vol 2 (01) ◽  
pp. 33-36 ◽  
Author(s):  
T. R. Preston ◽  
E. Rattray ◽  
F. White
Keyword(s):  
Histological Examination ◽  
Alimentary Tract ◽  
Clinical Symptoms ◽  
Skim Milk ◽  
Final Concentration ◽  
Post Mortem ◽  
Severe Damage ◽  
Whole Milk

1. 2-week-old calves previously fed whole milk were changed abruptly o t skim milk which had been treated with formalin to give a final concentration of 0·1% of the preservative.2. Difficulty was experienced in accustoming the calves to drink such formalin-treated milk and scouring invariably occurred within two days of the changeover.3. Post-mortem and histological examination of calves fed formalin-treated skim milk showed severe damage to the alimentary tract compatible with clinical symptoms.4. None of the above findings was apparent in control calves fed untreated skim milk.

Ultrastructure of the lesion induced by toxin T-514 isolated from K. humboldtiana in the alveolar region of the lung

1992 ◽  
Vol 50 (1) ◽  
pp. 640-641
Author(s):  
Julio Sepúlveda-Saavedra ◽  
Beatriz González-Corona ◽  
Víctor A. Tamez Rodríguez ◽  
Ma. Victoria Bermúdez de Rocha ◽  
Alfredo Piñeyro López
Keyword(s):  
Electron Microscope ◽  
Guinea Pig ◽  
Macaca Fascicularis ◽  
Osmium Tetroxide ◽  
Lead Citrate ◽  
Severe Damage ◽  
Thin Sections ◽  
Alveolar Region ◽  
Histopathological Findings

It has been shown in previous studies that the toxin T-514 isolated from K. humboldtiana induces severe damage to the lung in treated rodents. Histopathological findings include edema, and alveolar hemorrage. However, the ultraestructure of the lesion has not been investigated. In this study we used two species of rodents: Hamster and guinea pig, and a primate: Macaca fascicularis. Animals received different single dosis of the toxin via intraperitoneal. Control animals received only the vehicle (propylen glycol). Inmediately after spontaneous death, lung samples were fixed in Karnovsky-Ito fixative, post fixed in osmium tetroxide and embedded in epon. Thin sections were prepared with an Ultratome V LKB, stained with uranly acetate and lead citrate, and studied in an electron microscope Zeiss-EM109.

Hemangiomatosis of the greater omentum – a case report

2019 ◽  
Vol 98 (4) ◽  
pp. 178-180
Keyword(s):  
Case Report ◽  
Surgical Treatment ◽  
Histological Examination ◽  
Case Reports ◽  
Greater Omentum ◽  
Surgical Removal ◽  
Consumption Coagulopathy ◽  
Cavernous Hemangiomas ◽  
Mesodermal Origin ◽  
Benign Tumours

Cavernous hemangiomas are benign tumours of mesodermal origin. Even though various localizations of hemangioma have been described in the literature, its occurrence in the greater omentum is very rare. Only symptomatic hemangiomas are indicated for surgical treatment. There are case reports presenting resection or surgical removal of the greater omentum with hemangioma because of mechanical syndrome, consumption coagulopathy, bleeding, infection or suspicion of a malignancy. This article presents a case report of a patient operated on for a suspicion of carcinomatosis of the greater omentum. Histological examination found hemangiomatosis in the resected greater omentum.

Clinical case of breast tumor relapse in domestic cat

2020 ◽  
pp. 29-32
Author(s):  
Viktor V. Grechko ◽  
◽  
Lyudmila F. Bodrova ◽  
Dmitriy K. Ovchinnikov ◽  
Keyword(s):  
Histological Examination ◽  
Breast Tumor ◽  
Abdominal Ultrasound ◽  
Liver Parenchyma ◽  
Mammary Glands ◽  
Blood Analysis ◽  
Domestic Cat ◽  
Biological Behavior ◽  
Breast Adenocarcinoma ◽  
Low Grade

Domestic shorthair 10-year-old cat, not sterilized, not vaccinated, feeding from the table. We went to the clinic with breast cancer. General and special research methods were performed: blood analysis, x-ray examination of the lungs and abdominal ultrasound. Based on anamnesis and clinical examination, the diagnosis was made: a malignant breast tumor. The operation was performed. The operating material is sent for histological examination. Chemotherapy was prescribed. After the fi rst chemotherapy and monitoring of the animal's condition, it was necessary to perform a second unilateral mastoectomy of the remaining mammary glands, but the animal's owners refused to perform the operation. Chemotherapy was performed twice. Re-applied, after a year and a half with a sharp deterioration in the General condition and the appearance of a new tumor on the non-removed mammary glands. The owners refused the necessary treatment and decided to euthanize the cat. At the autopsy, breast neoplasms are presented as tubercles of various sizes. There are pronounced signs of inflammation, ulceration and necrosis foci. In the chest cavity, the lungs are enlarged, there were single and multiple formations of white and brown color, round shape of different diameters, dense consistency. There is a large number of metastases in the liver parenchyma. The organ is enlarged in size, dark brown in color, irregularly colored, with an uneven (bumpy) surface. In General, there are many dense nodes of a round-oval shape of white color, of various diameters. Histological examination of the tumor revealed foci of necrosis, atypical glandular complexes with frequently occurring mitosis figures. This structure indicates a low-grade breast adenocarcinoma. In the lungs, there is an expansion of the alveoli and bronchioles, thinning and rupture of the interalveolar partitions. There is a complex of tumor cells. In the liver, there is a lack of structural units of the liver, hepatocytes disperse chaotically, liver triads do not have a clear localization, atrophy and necrosis are expressed. Metastases in the liver, various forms. In a cat, a breast tumor interpreted as an adenocarcinoma had different biological behavior. At the initial diagnosis-adenocarcinoma of medium differentiation, and a year and a half later-low-grade adenocarcinoma.

HISTOLOGICAL CHANGES IN THE INTESTINES OF POULTRY DURING FODDER INTOXICATION

2020 ◽  
Author(s):  
E.P. Dolgov ◽  
◽  
A.A. Abramov ◽  
E.V. Kuzminova ◽  
E.V. Rogaleva ◽  
...  
Keyword(s):  
Body Weight ◽  
Histological Examination ◽  
Aflatoxin B1 ◽  
Structural Changes ◽  
Clinical Manifestations ◽  
Feed Additives ◽  
The Body ◽  
Histological Changes ◽  
Beet Pulp

The article presents the data on the study of the influence of mycotoxins combination (T-2 toxin at the concentration of 0.095 mg/kg and aflatoxin B1 in the concentration of 0.019 mg/kg) on the body of quails and the results of pharmacocorrection of toxicosis with a complex consisting of beet pulp and lecithin. Structural changes in the intestines of quais at fodder mycotoxicosis are described. The use of antitoxic feed additives in poultry led to a weakening of the action of xenobiotics, which was confirmed by an increase in the safety of poultry and increase in body weight of quails, a decrease in the clinical manifestations of intoxication, as well as in positive changes in the structure of the intestine of the poultry during histological examination.

CYTOLOGICAL DIAGNOSIS AS A METHOD OF SCREENING OF PRE-MALIGNANT MELANOCYTIC TUMORS AND EARLY FORMS OF SKIN MELANOMA

2018 ◽  
Vol 64 (3) ◽  
pp. 384-387
Author(s):  
Viktor Novik ◽  
D. Dreval
Keyword(s):  
Laser Therapy ◽  
Histological Examination ◽  
Clinical Data ◽  
Surgical Excision ◽  
Cytological Diagnosis ◽  
Skin Melanoma ◽  
Cytological Examination ◽  
Melanocytic Tumors ◽  
Quality Character ◽  
Atypical Nevi

Cytohistological comparisons to the account of the clinical data and revision of cytological and histological preparations on a material received from 21 patients are made. Cytomorphological features of juvenile nevi (Spitz-nevus, Reed-nevus), dysplastic and atypical nevi and early forms of melanoma are described. The establishment at cytological examination of good-quality character of melanocytic defeats at the account of the clinical data could be the basis for appointment laser therapy. At revealing of atypical melanocytes in cytological preparations patients should be referred to specialized oncological institutions for surgical excision of tumor with the subsequent histological examination. Thus cytological examination could be used in dermatological practice as a method of screening pre-malignant melanocytic tumors and skin melanoma.

Winter Survival Evaluation of 172 Groundcovers in Northern Illinois

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 453d-453
Author(s):  
A.M. Shirazi ◽  
E.A. HedBorn ◽  
S.A. Mehaffey ◽  
A.S. Merritt
Keyword(s):  
Cold Resistance ◽  
Winter Hardiness ◽  
Winter Survival ◽  
Severe Damage ◽  
Hedera Helix ◽  
Cold Temperatures ◽  
Ajuga Reptans ◽  
Significant Damage ◽  
Cold Damage ◽  
Euonymus Fortunei

The winter hardiness of many groundcover cultivars in northern Illinois is not well-known. This study was designed to evaluate the survival of 172 plants used in the groundcover path at The Morton Arboretum. Once a month, from Sept.1997 to Jan. 1998, the plants chosen for this study were visually evaluated and their vitality rated on a scale of 1 to 5 (1 = alive, 5 = dead). All nine cultivars of Euonymus fortunei remained virtually unchanged throughout the study period. Among six cultivars of Hedera helix, only `Gold Heart' showed minor damage in November. Nine Heuchera were evaluated and all exhibited excellent resistance to cold temperatures. While all the Pulmonarias studied showed some cold damage by November, `Bielefeld Pink', `Little Blue', `Roy Davidson', Pulmonaria longifolia var. cevennensis, and Pulmonaria officinalis `Sissinghurst White' fared the best for the longest period of time. Five cultivars of Pachysandra terminalis were included in this study. None had significant damage until November, and then only rated a “2.” Of the eight Ajuga evaluated, Ajuga pyramidalis `Metallica Crispa', and Ajuga reptans `Braunherz', `Catlin's Giant', and `Gaiety', exhibited the best cold resistance. Four Polygonums varied widely in their response to cold temperatures, but all showed signs of severe damage in November. Polygonum `Border Jewel' exhibited the best tolerance, rating a “1” in October, but in November it was given a rating of “4.” Their recovery in spring will be compared.

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