scholarly journals Nuclear RNA regulation by XRN2 and XTBD family proteins

2021 ◽  
Author(s):  
İlkin Aygün ◽  
Takashi S. Miki
Keyword(s):  
Rna Regulation ◽  
Nuclear Rna

THE NUCLEAR RNA-SYNTHESIS IN THE ANTERIOR PITUITARY OF RATS

1965 ◽  
Vol 49 (3_Suppl) ◽  
pp. S160 ◽  
Author(s):  
E. Stöcker ◽  
G. Dhom
Keyword(s):  
Anterior Pituitary ◽  
Rna Synthesis ◽  
Nuclear Rna Synthesis ◽  
Nuclear Rna

RNA Regulation by Estrogen

2011 ◽  
Author(s):  
J. A. Berglund ◽  
Rodger Voelker ◽  
Paul Barber ◽  
Julien Diegel ◽  
Amy Mahady ◽  
...  
Keyword(s):  
Rna Regulation

scholarly journals MAC5, an RNA-binding protein, protects pri-miRNAs from SERRATE-dependent exoribonuclease activities

2020 ◽  
Vol 117 (38) ◽  
pp. 23982-23990 ◽  
Author(s):  
Shengjun Li ◽  
Mu Li ◽  
Kan Liu ◽  
Huimin Zhang ◽  
Shuxin Zhang ◽  
...  
Keyword(s):  
Rna Binding ◽  
Rna Binding Protein ◽  
Dual Role ◽  
Mirna Biogenesis ◽  
Core Component ◽  
Stem Loop ◽  
Loop Region ◽  
Nuclear Rna ◽  
Efficient Processing ◽  
The Stability

MAC5 is a component of the conserved MOS4-associated complex. It plays critical roles in development and immunity. Here we report that MAC5 is required for microRNA (miRNA) biogenesis. MAC5 interacts with Serrate (SE), which is a core component of the microprocessor that processes primary miRNA transcripts (pri-miRNAs) into miRNAs and binds the stem-loop region of pri-miRNAs. MAC5 is essential for both the efficient processing and the stability of pri-miRNAs. Interestingly, the reduction of pri-miRNA levels inmac5is partially caused by XRN2/XRN3, the nuclear-localized 5′-to-3′ exoribonucleases, and depends on SE. These results reveal that MAC5 plays a dual role in promoting pri-miRNA processing and stability through its interaction with SE and/or pri-miRNAs. This study also uncovers that pri-miRNAs need to be protected from nuclear RNA decay machinery, which is connected to the microprocessor.

scholarly journals Nuclear RNA purification by flow cytometry to study nuclear processes in plants

2021 ◽  
Vol 2 (1) ◽  
pp. 100320
Author(s):  
Belén Moro ◽  
Malgorzata Kisielow ◽  
Veronica Barragan Borrero ◽  
Antoine Bouet ◽  
Christopher A. Brosnan ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Rna Purification ◽  
Nuclear Rna ◽  
Nuclear Processes

scholarly journals Characterization of the nuclear and cytosolic transcriptomes in human brain tissue reveals new insights into the subcellular distribution of RNA transcripts

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ammar Zaghlool ◽  
Adnan Niazi ◽  
Åsa K. Björklund ◽  
Jakub Orzechowski Westholm ◽  
Adam Ameur ◽  
...  
Keyword(s):  
Human Brain ◽  
Expression Analysis ◽  
Differential Expression Analysis ◽  
Adult Brain ◽  
Sequencing Data ◽  
Human Brain Tissue ◽  
Protein Coding ◽  
Rna Transcripts ◽  
Nuclear Rna ◽  
The Impact

AbstractTranscriptome analysis has mainly relied on analyzing RNA sequencing data from whole cells, overlooking the impact of subcellular RNA localization and its influence on our understanding of gene function, and interpretation of gene expression signatures in cells. Here, we separated cytosolic and nuclear RNA from human fetal and adult brain samples and performed a comprehensive analysis of cytosolic and nuclear transcriptomes. There are significant differences in RNA expression for protein-coding and lncRNA genes between cytosol and nucleus. We show that transcripts encoding the nuclear-encoded mitochondrial proteins are significantly enriched in the cytosol compared to the rest of protein-coding genes. Differential expression analysis between fetal and adult frontal cortex show that results obtained from the cytosolic RNA differ from results using nuclear RNA both at the level of transcript types and the number of differentially expressed genes. Our data provide a resource for the subcellular localization of thousands of RNA transcripts in the human brain and highlight differences in using the cytosolic or the nuclear transcriptomes for expression analysis.

7SK small nuclear RNA inhibits cancer cell proliferation through apoptosis induction

Tumor Biology ◽  
2014 ◽  
Vol 36 (4) ◽  
pp. 2809-2814 ◽  
Author(s):  
Farid Keramati ◽  
Ehsan Seyedjafari ◽  
Parviz Fallah ◽  
Masoud Soleimani ◽  
Hossein Ghanbarian
Keyword(s):  
Cell Proliferation ◽  
Cancer Cell ◽  
Cancer Cell Proliferation ◽  
Small Nuclear Rna ◽  
Apoptosis Induction ◽  
Nuclear Rna

scholarly journals Upregulation of Vitamin C Transporter Functional Expression in 5xFAD Mouse Intestine

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 617
Author(s):  
Trevor Teafatiller ◽  
Christopher W. Heskett ◽  
Anshu Agrawal ◽  
Jonathan S. Marchant ◽  
Janet E. Baulch ◽  
...  
Keyword(s):  
Mouse Model ◽  
Functional Expression ◽  
Specific Protein ◽  
Glutathione Peroxidase 1 ◽  
Stress Response Genes ◽  
Nuclear Rna ◽  
The Status ◽  
5Xfad Mouse ◽  
Mouse Intestine ◽  
5Xfad Mice

The process of obtaining ascorbic acid (AA) via intestinal absorption and blood circulation is carrier-mediated utilizing the AA transporters SVCT1 and SVCT2, which are expressed in the intestine and brain (SVCT2 in abundance). AA concentration is decreased in Alzheimer’s disease (AD), but information regarding the status of intestinal AA uptake in the AD is still lacking. We aimed here to understand how AA homeostasis is modulated in a transgenic mouse model (5xFAD) of AD. AA levels in serum from 5xFAD mice were markedly lower than controls. Expression of oxidative stress response genes (glutathione peroxidase 1 (GPX1) and superoxide dismutase 1 (SOD1)) were significantly increased in AD mice jejunum, and this increase was mitigated by AA supplementation. Uptake of AA in the jejunum was upregulated. This increased AA transport was caused by a marked increase in SVCT1 and SVCT2 protein, mRNA, and heterogeneous nuclear RNA (hnRNA) expression. A significant increase in the expression of HNF1α and specific protein 1 (Sp1), which drive SLC23A1 and SLC23A2 promoter activity, respectively, was observed. Expression of hSVCT interacting proteins GRHPR and CLSTN3 were also increased. SVCT2 protein and mRNA expression in the hippocampus of 5xFAD mice was not altered. Together, these investigations reveal adaptive up-regulation of intestinal AA uptake in the 5xFAD mouse model.

scholarly journals 6S RNA regulation of relA alters ppGpp levels in early stationary phase

Microbiology ◽  
2010 ◽  
Vol 156 (12) ◽  
pp. 3791-3800 ◽  
Author(s):  
Amy T. Cavanagh ◽  
Pete Chandrangsu ◽  
Karen M. Wassarman
Keyword(s):  
Amino Acid ◽  
Stationary Phase ◽  
Regulation Of Transcription ◽  
Early Stationary Phase ◽  
Rna Regulation ◽  
Global Regulators ◽  
Expression Of Genes ◽  
Non Coding Rna ◽  
6S Rna ◽  
Altered Regulation

6S RNA is a small, non-coding RNA that interacts directly with σ 70-RNA polymerase and regulates transcription at many σ 70-dependent promoters. Here, we demonstrate that 6S RNA regulates transcription of relA, which encodes a ppGpp synthase. The 6S RNA-dependent regulation of relA expression results in increased ppGpp levels during early stationary phase in cells lacking 6S RNA. These changes in ppGpp levels, although modest, are sufficient to result in altered regulation of transcription from σ 70-dependent promoters sensitive to ppGpp, including those promoting expression of genes involved in amino acid biosynthesis and rRNA. These data place 6S RNA as another player in maintaining appropriate gene expression as cells transition into stationary phase. Independent of this ppGpp-mediated 6S RNA-dependent regulation, we also demonstrate that in later stationary phase, 6S RNA continues to downregulate transcription in general, and specifically at a subset of the amino acid promoters, but through a mechanism that is independent of ppGpp and which we hypothesize is through direct regulation. In addition, 6S RNA-dependent regulation of σ S activity is not mediated through observed changes in ppGpp levels. We suggest a role for 6S RNA in modulating transcription of several global regulators directly, including relA, to downregulate expression of key pathways in response to changing environmental conditions.

Studies on the effects of chemicals on the processing of nuclear RNA

1971 ◽  
Vol 20 (5) ◽  
pp. 1029-1033 ◽  
Author(s):  
Michael B. Sporn
Keyword(s):  
Nuclear Rna
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