Radiotherapy of the neck influences the distribution of laryngeal secretory glands

2005 ◽  
Vol 119 (12) ◽  
pp. 976-980 ◽  
Author(s):  
Tadashi Nakashima ◽  
Hideichiro Tomita ◽  
Sachio Tsuda ◽  
Shun-ichi Chitose

To elucidate the influence of radiation therapy on the larynx in patients who receive radiotherapy to the neck, we observed the changes in the distribution of the laryngeal glands. Human adult larynges from patients with either laryngeal or hypopharyngeal cancer were histopathologically examined, and the changes in the glandular distribution as well as in the ratio of serous or mucous type cells were surveyed using an image analysis system. The mean ratio of the area occupied by glandular acini in the subglottic larynx was 0.182 in the non-radiation group and 0.098 in the radiation group (p < 0.001). Although no statistical difference was observed, the average density of the glands decreased and the average ratio of serous-type/mucous-type glandular cells decreased in the irradiated larynx. The glandular acini are often replaced with fibrous connective tissue and the ratio of serous-type glandular cells decreases. These results indicate that not only the voice function but also the local defence function of the larynx might be impaired after radiotherapy.

2012 ◽  
Vol 472-475 ◽  
pp. 1963-1966
Author(s):  
Jin Sun ◽  
Xiao Bo Wang ◽  
Xiao Jing Wang ◽  
Yan Lin ◽  
Zhen Zhong Gao

In this paper, anatomical characters and tissue proportion of fast-growing Toona ciliata samples from Guangzhou was investigated assisted with the light microscope and image analysis system based on the principle of stereology. Qualitative and quantitative data were presented for purposes of identification. Results showed that fast growing T. ciliata was coarse texture with wide growth ring of 3~5cm.The ray proportion was 12.12%-25.56%, the mean value was 17.98%, increasing with time-dependent relevance. The vessel-elements proportion was 6.37%-10.00%, the mean value was 7.40%, increasing then declining and finally increasing. The fiber and other tissue proportion was 67.06%-79.92%, the mean value was 74.62%, declining by year.


1982 ◽  
Vol 30 (1) ◽  
pp. 49-57 ◽  
Author(s):  
R J Sklarew

A Quantimet 720 Image-Analysis System has been configured and programmed to enumerate silver grains over labeled nuclei in Feulgen-stained autoradiographs. The accuracy and reproducibility of the estimates have been documented in 3H-thymidine (3HTdR)-labeled rat kidney cell cultures. The Quantimet and visual grain count estimates showed excellent correlation over a wide range of counts and was independent of grain density and clustering pattern. One advantage of this approach is that the grain counts can be related to specific cellular structures. The simultaneous measurement of the mean optical density of the grain-free nucleus provides a way of evaluating 3HTdR-absorption effects for valid grain count comparisons. Using a light pen, about 800 cells are measured per hour. The grain count estimates may be made simultaneously with estimates of Feulgen-stained DNA content in the labeled nuclei. This automated technology opens new avenues for critical applications to cell cycle analysis and related problems.


Neurosurgery ◽  
2010 ◽  
Vol 66 (2) ◽  
pp. 385-388 ◽  
Author(s):  
R. Shane Tubbs ◽  
Christoph J. Griessenauer ◽  
Marios Loukas ◽  
Mohammadali M. Shoja ◽  
Aaron A. Cohen-Gadol

Abstract OBJECTIVE To further elucidate the importance of anatomic variations in morphology of the foramen magnum and associated clinical implications, we conducted a morphometric study. METHODS Seventy-two dry skulls were used for this study. Digital images were obtained of the foramen magnum from an inferior view. These images were studied using a computer-assisted image analysis system. Next, an image processor was used to calculate pixel differences between 2 selected points, which allowed accurate translation of pixel differences into metric measurements. RESULTS We found that the mean surface area of the foramen magnum was 558 mm2, the mean anteroposterior diameter was 3.1 cm, and the mean horizontal diameter was 2.7 cm. For comparison, surface areas were classified into 3 types based on size. Type I foramina were identified in 20.8% of the dry skulls (15 skulls) and exhibited a surface area of less than 500 mm2. Type II (66.6%, 48 skulls) was applied to foramina of an intermediate size with surface areas ranging between 500 to 600 mm2. Type III (12.5%, 9 skulls) was applied to large foramina with surface areas of more than 600 mm2. CONCLUSION These data may be of use as a morphometric database for description of “normal” variants of foramen magnum morphology.


2018 ◽  
Vol 43 (2) ◽  
pp. 151-161 ◽  
Author(s):  
R Pilo ◽  
M Folkman ◽  
A Arieli ◽  
S Levartovsky

SUMMARY The absolute marginal gap (AMG) precementation and postcementation and the retention of zirconia crowns cemented to standardized molar preparations (4×10) by self-adhesive resin cements (SARCs) were evaluated. The following SARCs were used: RelyX U-200 (RXU200; 3M ESPE, Seefeld, Germany), SmartCem 2 (SC2; Dentsply, Milford, DE, USA), and G-Cem Automix (GCA; GC, Alsip, IL, USA). The control adhesive resin cement was Panavia 21 (PAN; Kuraray Dental Co Ltd, Osaka, Japan). Twenty measuring locations at a constant interval along the margins were marked, and the AMG was measured by an image analysis system connected to a stereomicroscope (20×). The cemented copings were aged 270 days at 100% humidity and 37°C and then underwent 10,000 thermal cycles, 5°C-55°C. After aging, the crowns were tested for retention, and the debonded surfaces were examined at 3× magnification. The mean marginal gaps precementation and postcementation were 34.8 ± 17.4 μm and 72.1 ± 31 μm, respectively, with no statistically significant differences between the cements. A significant difference (p≤0.001) in retention between the cements was found. The highest values were obtained for SC2 and GCA (1385 Pa and 1229 Pa, respectively), but these presented no statistically significant differences. The lowest values were found for PAN and RXU200 (738 Pa and 489 Pa, respectively), but these showed no statistically significant differences. The predominant mode of failure in all of the groups was mixed, and no correlations were found between marginal gap and retention.


Author(s):  
A. V. Crewe ◽  
M. Ohtsuki

We have assembled an image processing system for use with our high resolution STEM for the particular purpose of working with low dose images of biological specimens. The system is quite flexible, however, and can be used for a wide variety of images.The original images are stored on magnetic tape at the microscope using the digitized signals from the detectors. For low dose imaging, these are “first scan” exposures using an automatic montage system. One Nova minicomputer and one tape drive are dedicated to this task.The principal component of the image analysis system is a Lexidata 3400 frame store memory. This memory is arranged in a 640 x 512 x 16 bit configuration. Images are displayed simultaneously on two high resolution monitors, one color and one black and white. Interaction with the memory is obtained using a Nova 4 (32K) computer and a trackball and switch unit provided by Lexidata.The language used is BASIC and uses a variety of assembly language Calls, some provided by Lexidata, but the majority written by students (D. Kopf and N. Townes).


Author(s):  
D.S. DeMiglio

Much progress has been made in recent years towards the development of closed-loop foundry sand reclamation systems. However, virtually all work to date has determined the effectiveness of these systems to remove surface clay and metal oxide scales by a qualitative inspection of a representative sampling of sand particles. In this investigation, particles from a series of foundry sands were sized and chemically classified by a Lemont image analysis system (which was interfaced with an SEM and an X-ray energy dispersive spectrometer) in order to statistically document the effectiveness of a reclamation system developed by The Pangborn Company - a subsidiary of SOHIO.The following samples were submitted: unreclaimed sand; calcined sand; calcined & mechanically scrubbed sand and unused sand. Prior to analysis, each sample was sprinkled onto a carbon mount and coated with an evaporated film of carbon. A backscattered electron photomicrograph of a field of scale-covered particles is shown in Figure 1. Due to a large atomic number difference between sand particles and the carbon mount, the backscattered electron signal was used for image analysis since it had a uniform contrast over the shape of each particle.


2018 ◽  
Author(s):  
F.B. Musaev ◽  
N.S. Priyatkin ◽  
M.V. Arkhipov ◽  
P.A. Shchukina ◽  
A.F. Bukharov ◽  
...  

Приведено описание разработанной авторами методики цифровой компьютерной морфометрии семян овощных культур на основе системы анализа изображений, состоящей из планшетного сканера и программного обеспечения для автоматических измерений. В основу метода положено представление о разнокачественности семян, обусловленной генетической неоднородностью самих семенных растений, используемых в промышленном семеноводстве. Физические свойства семян (их форма и линейные размеры) – основные параметры при определении их качества. Цифровые изображения семян получены при помощи планшетного сканера HP Sсanjet 200 на базе Агрофизического НИИ с использованием серийного программного обеспечения «Argus-BIO», производства ООО «АргусСофт» (г. Санкт-Петербург). Метод состоит из подбора контрастной подложки (фона) для сканирования семян с минимальными теневыми эффектами, калибровку программного обеспечения для привязки к истинным размерным величинам, подбор параметров измерений и автоматическое распознавание цифровых сканированных изображений семян. Представлены экспериментальные данные по морфометрии экологически разнокачественных семян фасоли овощной, матрикально разнокачественных семян укропа, пастернака и лука Кристофа. Семена укропа и пастернака, собранные из разных порядков ветвления семенного растения, значительно различались по величине линейных параметров. Наиболее показательный линейный параметр семян – площадь проекции. Предложенная авторами методика цифровой морфометрии, уже использована на практике и в перспективе может быть задействована в исследованиях экологической и матрикальной разнокачественности семян овощных культур. Так, она прошла апробацию на разнокачественных семенах пяти сортов фасоли овощной (Настена, Магура, Миробела, Морена, Бажена) полученных в пяти контрастных эколого-географических условиях среды (Москва, Белгород, Ставрополь, Омск, Горки) в 2011–2012 годах. В дальнейшем методика может быть использована для улучшения качества цифровых изображений семян, изучения разнокачественности семян в том числе и для совершенствования контроля за селекционным процессом. Кроме того, она применима для изучения взаимосвязи совокупности морфометрических характеристик семян и их посевных качеств.The description of the method of digital computer morphometry of vegetable seeds developed by the authors on the basis of the image analysis system consisting of a flatbed scanner and software for automatic measurements is given. The method is based on the idea of seed quality, due to the genetic heterogeneity of the seed plants used in industrial seed production. Physical properties of seeds (their shape and linear dimensions) are the main parameters in determining their quality. Digital image of the seed obtained using the flatbed scanner, HP Sсanjet 200 on the basis of the Agrophysical research Institute with serial software “Argus-BIO”, produced by LLC “Argussoft” (Saint-Petersburg). The method consists of selection of a contrast substrate (background) for scanning seeds with minimal shadow effects, calibration of software for binding to true size values, selection of measurement parameters and automatic recognition of digital scanned images of seeds. Experimental data on the morphometry of ecologically different-quality seeds of vegetable beans, matrix seeds of dill, Pasternak and Christoph onion are presented. Seeds of dill and parsnip, collected from different orders of branching of the seed plant, significantly differed in size of linear parameters. The most revealing linear parameter seed – area projection. The method of digital morphometry proposed by the authors has already been used in practice and in the future can be used in studies of ecological and matrix heterogeneity of vegetable seeds. So, it was tested on different quality seeds of five varieties of vegetable beans (Nastena, Magura, Mirobelа, Morena, Bazhenf) obtained in five contrasting environmental and geographical conditions (Moscow, Belgorod, Stavropol, Omsk, Gorki) in 2011-2012. In the future, the technique can be used to improve the quality of digital images of seeds, study of seed diversity, including to improve the control of the breeding process. In addition, it is applicable to study the relationship of the set of morphometric characteristics of seeds and their sowing qualities.


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