Enhanced Release of Chitosanase from Streptomyces griseus through Direct Interaction of Liposome with Cell Membrane under Heat Stress

Heat and drought are among the major obstacles confronting crop production under climate change. The present study was conducted to evaluate 50 diverse wheat genotypes for cell membrane stability (CMS) and chlorophyll content at seedling and anthesis stages under heat and drought stress conditions, to understand the effect of the two abiotic factors and to find promising genotypes for future breeding. Experiments were conducted in the glasshouse (seedling stage) and the field (anthesis stage). Analysis of variance showed significant variation (P ≤ 0.05) for all of the traits at seedling and anthesis stages. High levels of broad-sense heritability and genetic advance at 5% selection intensity indicated the presence of a high genetic component of variation and potential for genetic improvement through selection among the existing genetic variation. CMS showed a significant positive correlation with 1000-grain weight (TGW) under heat and drought conditions at both seedling and anthesis stages. Chlorophyll a/b ratio at seedling stage exhibited a significant negative correlation (r = –0.39, P < 0.05) with TGW under heat stress. Total chlorophyll content was significantly (r = 0.42, P < 0.05) correlated with TGW under heat stress at anthesis. Genotypes ETAD248 and ETAD7 showed the highest CMS and TGW values, whereas their chlorophyll a/b values were lowest, at both seedling and anthesis stages under heat and drought stress conditions. Higher CMS and total chlorophyll content, and lower chlorophyll a/b, were found to be useful indicators to identify genotypes with high TGW under heat and drought stress conditions. This study indicated the possibility of using seedling resistance as an indicator for later stage response in breeding for heat and drought resistance. The resistant genotypes identified can be used as potential germplasm in breeding programs.

Download Full-text

Resistance to tamoxifen-induced apoptosis is associated with direct interaction between Her2/neu and cell membrane estrogen receptor in breast cancer

International Journal of Cancer ◽

10.1002/ijc.1614 ◽

2001 ◽

Vol 97 (3) ◽

pp. 306-312 ◽

Cited By ~ 85

Author(s):

Yih-Lin Chung ◽

Meei-Ling Sheu ◽

Shun-Chun Yang ◽

Chi-Hung Lin ◽

Sang-Hue Yen

Keyword(s):

Breast Cancer ◽

Estrogen Receptor ◽

Cell Membrane ◽

Direct Interaction ◽

Induced Apoptosis

Download Full-text

Wnt signaling related transcripts and their relationship to energy metabolism in C2C12 myoblasts under temperature stress

PeerJ ◽

10.7717/peerj.11625 ◽

2021 ◽

Vol 9 ◽

pp. e11625

Author(s):

Marua Abu Risha ◽

Asghar Ali ◽

Puntita Siengdee ◽

Nares Trakooljul ◽

Fiete Haack ◽

...

Keyword(s):

Heat Stress ◽

Energy Metabolism ◽

Cell Membrane ◽

Cold Stress ◽

Wnt Signaling ◽

Signaling Pathways ◽

Signaling Pathway ◽

Temperature Stress ◽

C2c12 Myoblasts ◽

Severe Heat Stress

Temperature stress is one of the main environmental stressors affecting the welfare, health and productivity of livestock. Temperature changes can modify cell membrane components, disrupting the crosstalk between the cell and its surroundings by affecting signaling pathways including Wnt signaling pathway, which subsequently disrupts cell energy metabolism. The present study aims to understand the effect of temperature stress on the expression of genes involved in Wnt signaling pathways, and their interaction with energy metabolism in C2C12 myoblasts cells. The C2C12 cells were exposed to cold stress (35 °C), mild heat stress (39 °C) and severe heat stress (41 °C), whereas 37 °C was used as control temperature. Transcript levels of important genes involved in Wnt signaling including Axin2, Tnks2, Sfrp1, Dkk1, Dact1, Cby1, Wnt5a, Wnt7a, Wnt11, Porcn, Ror2, Daam1, and Ppp3ca were significantly altered under severe heat stress (41 °C), whereas eight Wnt signaling-related transcripts (Daam1, Ppp3ca, Fzd7, Wnt5a, Porcn, Tnks2, Lrp6, and Aes) were significantly altered under cold stress (35 °C) compared to control. Under heat stress transcripts of the Wnt/β-catenin inhibitors (Sfrp1, Dkk1, and Cby1) and negative regulators (Dact1 and Axin2) are activated. A positive correlation between oxidative phosphorylation and Wnt-related transcripts was found under high temperatures. Transcripts of the cell membrane receptors, including Lrp6 and Fzd7, and the members of Wnt/Ca+2 signaling pathway, including Ppp3ca and Porcn were downregulated under cold stress. Many Wnt signaling-related transcripts were positively correlated with glycolysis under cold stress. These findings indicate a cross-talk between Wnt signaling and energy metabolism under thermal stress.

Download Full-text

Cell Membrane Stability and Relative Cell Injury in Response to Heat Stress during Early and Late Seedling Stages of Diverse Carrot (Daucus carota L.) Germplasm

HortScience ◽

10.21273/hortsci15058-20 ◽

2020 ◽

Vol 55 (9) ◽

pp. 1446-1452 ◽

Cited By ~ 1

Author(s):

Aneela Nijabat ◽

Adam Bolton ◽

Muhammad Mahmood-ur-Rehman ◽

Adeel Ijaz Shah ◽

Rameez Hussain ◽

...

Keyword(s):

Heat Stress ◽

Cell Membrane ◽

Heat Tolerance ◽

Daucus Carota ◽

Cell Injury ◽

Membrane Stability ◽

Seedling Stage ◽

Stress Indicators ◽

Cell Membrane Stability ◽

Heat Tolerant

Heat waves occur with more regularity and they adversely affect the yield of cool season crops including carrot (Daucus carota L.). Heat stress influences various biochemical and physiological processes including cell membrane permeability. Ion leakage and increase in cell permeability are indicators of cell membrane stability and have been used to evaluate the stress tolerance response in numerous crops and inform plant breeders for improving heat tolerance. No study has been published about the effects of heat stress on cell membrane stability and relative cell injury of carrot. Therefore, the present study was designed to estimate these stress indicators in response to heat stress at the early and late seedling developmental stages of 215 diverse accessions of wild and cultivated carrot germplasm. The article identifies the relationship between early and late stages of seedling tolerance across carrot genotypes and identifies heat-tolerant genotypes for further genetic analysis. Significant genetic variation among these stress indicators was identified with cell membrane stability and relative cell injury ranging from 6.3% to 97.3% and 2.8% to 76.6% at the early seedling stage, respectively; whereas cell membrane stability and relative cell injury ranged from 2.0% to 94.0% and 2.5% to 78.5%, respectively, at the late seedling stage under heat stress. Broad-sense heritability ranged from 0.64 to 0.91 for traits of interest under study, which indicates a relatively strong contribution of genetic factors in phenotypic variation among accessions. Heat tolerance varied widely among both wild and cultivated accessions, but the incidence of tolerance was higher in cultivated carrots than in wild carrots. The cultivated carrot accessions PI 326009 (Uzbekistan), PI 451754 (Netherlands), L2450 (USA), and PI 502654 (Pakistan) were identified as the most heat-tolerant accessions with highest cell membrane stability. This is the first evaluation of cell membrane stability and relative cell injury in response to heat stress during carrot development.

Download Full-text

Effect of Late Seeding Warmer Condition on Phenology and Yield of Wheat

Bangladesh Agronomy Journal ◽

10.3329/baj.v24i1.55542 ◽

2021 ◽

Vol 24 (1) ◽

pp. 13-23

Author(s):

S Sharmin ◽

MA Hasan ◽

S Sikder

Keyword(s):

Heat Stress ◽

Cell Membrane ◽

Grain Yield ◽

Stress Condition ◽

Wheat Variety ◽

Yield Performance ◽

Heat Stress Condition ◽

Heat Tolerant ◽

Heat Susceptibility Index ◽

Hot Environments

Four wheat variety/genotype (BARI Gom-26, BAW-1202, BAW-1182 and BARI Gom-27) were tested under three heat stress regimes (normal, moderate and severe) to evaluate the effect of late seeding warmer condition on phenology and yield of wheat, as well as to identify suitable cultivars to develop heat-tolerant genotypes at Hajee Mohammad Danesh Science and Technology University (HSTU), Dinajpur during November, 2016 to April, 2017. Results revealed that genotype BAW-1182 and BAW-1202 showed greater thermostability of cell membrane with acceptable yield performance under heat stress condition. The order of tolerance based on heat susceptibility index (based on grain yield) was BAW-1182>BAW-1202>BARI Gom-27>BARI Gom-26 under heat stress conditions. Thus, BAW-1182 and BAW-1202 have the greatest potential to be used as high-yielding wheat genotypes under warm to hot environments and could be used in a breeding programme to develop heat-tolerant wheat. Bangladesh Agron. J. 2021, 24(1): 13-23

Download Full-text

Cell membrane stability- an important criterion for selection of heat tolerant genotypes in wheat (Triticum aestivum L.)

Journal of Applied and Natural Science ◽

10.31018/jans.v9i4.1458 ◽

2017 ◽

Vol 9 (4) ◽

pp. 1894-1900

Author(s):

Anzer Ul Islam ◽

Ashok K. Chhabra ◽

Satyaveer S. Dhanda ◽

Renu Munjal

Keyword(s):

Heat Stress ◽

Cell Membrane ◽

Grain Yield ◽

Grain Filling ◽

Triticum Aestivum L ◽

Membrane Stability ◽

Filling Rate ◽

Cell Membrane Stability ◽

Grain Filling Rate ◽

Heat Tolerant

Cell membrane stability, grain filling rate, grain filling duration, canopy temperature and grain yield were used to evaluate performance of 100 diverse bread wheat (Triticum aestivum L.) genotypes under timely sown and late sown heat stress conditions for two cropping season. The genotypes differed significantly for all the traits show-ing considerable variation for improvement of characters. The genotypes WH1165 had significant high grain yield (14.6* g and 11.4g) and (11.3* g and 11.4* g) followed by cell membrane stability under timely sown and heat stress conditions, respectively indicating potential tolerance against heat stress. Correlation coefficients revealed that cell membrane stability (0.451**) and (0.639**) in timely sown and in late sown conditions, respectively were the most important trait followed by grain filling rate (0.882** and 0.744**) under timely sown and late sown conditions respec-tively. Results revealed that bread wheat genotypes which had high value of cell membrane stability had high grain yield showed potential photorespiration and high grain filling rate under heat stress condition. Twenty two genotypes WH1021, WH1155, VL803, WH787, NW1014, Raj3765, HD1869, 2042, WH1124, HD2285, WH1133, HUW234, 4066, Sonak, UP2425, UP2473, PBW503, PBW373, PBW533, SGP13, HD2643 and WH789 were identified as heat tolerant genotypes based on their relative performance in yield components, grain yield and heat susceptibility indi-ces. These genotypes were found to be ideal candidates to be used in developing heat tolerant wheat varieties. Canopy temperature, membrane thermostability and grain filling rate have also shown strong correlation with grain yield. Because of this association, these traits constitute the best available ‘tool’ for genetic improvement of wheat suitable for cultivation under heat stressed environments. Thus, these could be used as indirect selection criteria for developing heat tolerant wheat genotypes that would provide sufficient yields to meet the ever increasing wheat demand.

Download Full-text

Cell membrane stability: Combining ability and gene effects under heat stress conditions

Cereal Research Communications ◽

10.1556/crc.37.2009.3.10 ◽

2009 ◽

Vol 37 (3) ◽

pp. 409-417 ◽

Cited By ~ 5

Author(s):

S. Dhanda ◽

R. Munjal

Keyword(s):

Heat Stress ◽

Cell Membrane ◽

Combining Ability ◽

Membrane Stability ◽

Stress Conditions ◽

Gene Effects ◽

Cell Membrane Stability

Download Full-text

Proximal C-terminal domain of sulphonylurea receptor 2A interacts with pore-forming Kir6 subunits in KATP channels

Biochemical Journal ◽

10.1042/bj20031087 ◽

2004 ◽

Vol 379 (1) ◽

pp. 173-181 ◽

Cited By ~ 14

Author(s):

Richard D. RAINBOW ◽

Marian JAMES ◽

Diane HUDMAN ◽

Mohammed AL JOHI ◽

Harprit SINGH ◽

...

Keyword(s):

Cell Membrane ◽

Katp Channel ◽

Direct Interaction ◽

Katp Channels ◽

Full Length ◽

Hek 293 ◽

Sulphonylurea Receptor ◽

Hek 293 Cells ◽

Terminal Domain ◽

293 Cells

Functional KATP (ATP-sensitive potassium) channels are hetero-octamers of four Kir6 (inwardly rectifying potassium) channel subunits and four SUR (sulphonylurea receptor) subunits. Possible interactions between the C-terminal domain of SUR2A and Kir6.2 were investigated by co-immunoprecipitation of rat SUR2A C-terminal fragments with full-length Kir6.2 and by analysis of cloned KATP channel function and distribution in HEK-293 cells (human embryonic kidney 293 cells) in the presence of competing rSUR2A fragments. Three maltose-binding protein–SUR2A fusions, rSUR2A-CTA (rSUR2A residues 1254–1545), rSUR2A-CTB (residues 1254–1403) and rSUR2A-CTC (residues 1294–1403), were co-immunoprecipitated with full-length Kir6.2 using a polyclonal anti-Kir6.2 antiserum. A fourth C-terminal domain fragment, rSUR2A-CTD (residues 1358–1545) did not co-immunoprecipitate with Kir6.2 under the same conditions, indicating a direct interaction between Kir6.2 and a 65-amino-acid section of the cytoplasmic C-terminal region of rSUR2A between residues 1294 and 1358. ATP- and glibenclamide-sensitive K+ currents were decreased in HEK-293 cells expressing full-length Kir6 and SUR2 subunits that were transiently transfected with fragments rSUR2A-CTA, rSUR2A-CTC and rSUR2A-CTE (residues 1294–1359) compared with fragment rSUR2A-CTD or mock-transfected cells, suggesting either channel inhibition or a reduction in the number of functional KATP channels at the cell surface. Anti-KATP channel subunit-associated fluorescence in the cell membrane was substantially lower and intracellular fluorescence increased in rSUR2A-CTE expressing cells; thus, SUR2A fragments containing residues 1294–1358 reduce current by decreasing the number of channel subunits in the cell membrane. These results identify a site in the C-terminal domain of rSUR2A, between residues 1294 and 1358, whose direct interaction with full-length Kir6.2 is crucial for the assembly of functional KATP channels.

Download Full-text