scholarly journals Υγιή αδέρφια παιδιών με καρκίνο

2020 ◽  
Vol 18 (2) ◽  
pp. 192
Author(s):  
Κ. Καραμπέλα ◽  
Π. Δ. Χατήρα ◽  
Δ. Δαμίγος
Keyword(s):  
Wilms Tumor ◽  
Myelogenous Leukemia ◽  
Traumatic Experiences ◽  
Self Awareness ◽  
Healthy Children ◽  
Emotional Instability ◽  
Emotional Life ◽  
Healthy Siblings ◽  
Health And Disease ◽  
Basic Purpose

The basic purpose of this research is to understand how healthy siblings visualize and represent health and disease as conditions affecting them and others in their family. Furthermore, an attempt was made to evaluate the emotions about health and disease for healthy siblings, as well as to investigate the parameters that interfere with the physicaland emotional life of healthy siblings. Fourteen healthy children participated in the research, aged between 7 and 14, siblings to children diagnosed and receiving treatment for leukemia (acute lymphoblastic &myelogenous leukemia), nephroblastoma (Wilms’ tumor), lymphoma, rhabdomyosarcoma, where evaluated according to the Hatira Projective Technique. Some positive but mainly negative responses were obtained.The negative reactions concerned emotional instability, rejection, separation, siblings’ rivalry, the medical treatment and the fear of death, while positive reactions concerned self-awareness, sensitization, earlier maturing and increased family coherence after the crisis. The bond between siblings can outrun time and crises, even outgrowing the relationship between parents and their children. It is simultaneously a very vulnerable relationship, since traumatic experiences such as those arising from childhood cancer interfere with the mental and emotional development of the healthy brother or sister.

HLA class I-restricted lysis of leukemia cells by a CD8+ cytotoxic T-lymphocyte clone specific for WT1 peptide

Blood ◽  
2000 ◽  
Vol 95 (1) ◽  
pp. 286-293 ◽  
Author(s):  
Hideki Ohminami ◽  
Masaki Yasukawa ◽  
Shigeru Fujita
Keyword(s):  
T Lymphocyte ◽  
Bone Marrow Cells ◽  
Cytotoxic T Lymphocyte ◽  
Wilms Tumor ◽  
Myelogenous Leukemia ◽  
Leukemia Cells ◽  
Target Antigen ◽  
Human Leukemia ◽  
Wt1 Gene ◽  
Normal Cells

Abstract The Wilms tumor (WT1) gene has been reported to be preferentially expressed in acute leukemia cells, regardless of leukemia subtype and chronic myelogenous leukemia cells in blast crisis, but not in normal cells. This finding suggests strongly that WT1 protein is a potential target of immunotherapy for human leukemia. In this study, we established a CD8+ cytotoxic T-lymphocyte (CTL) clone directed against a WT1-derived peptide and examined its immunologic actions on leukemia cells. A CD8+ CTL clone, designated TAK-1, which lysed autologous cells loaded with a WT1-derived 9-mer peptide consisting of the HLA-A24 (HLA-A*2402)-binding motifs was established by stimulating CD8+ T lymphocytes from a healthy individual repeatedly with WT1 peptide-pulsed autologous dendritic cells. TAK-1 was cytotoxic to HLA-A24–positive leukemia cells expressing WT1, but not to HLA-A24–positive lymphoma cells that did not express WT1, HLA-A24–negative leukemia cells, or HLA-A24–positive normal cells. Treating leukemia cells with an antisense oligonucleotide complementary to the WT1 gene resulted in reduced TAK-1-mediated cytotoxicity, suggesting that target antigen of TAK-1 on leukemia cells is the naturally processed WT1 peptide in the context of HLA-A24. TAK-1 did not inhibit colony formation by normal bone marrow cells of HLA-A24–positive individuals. Because WT1 is overexpressed ubiquitously in various types of leukemia cells, but not in normal cells, immunotherapy using WT1 peptide-specific CTL clones should be an efficacious treatment for human leukemia. (Blood. 2000;95:286-293)

Spirituality in expatriate experience and coping in mission

2020 ◽  
Vol 8 (2) ◽  
pp. 229-248
Author(s):  
Michał Wilczewski ◽  
Zbigniew Wróblewski ◽  
Mariusz Wołońciej ◽  
Arkadiusz Gut ◽  
Ewelina Wilczewska
Keyword(s):  
Narrative Analysis ◽  
Local Community ◽  
Personal Relationship ◽  
Traumatic Experiences ◽  
Self Awareness ◽  
Relationship With God ◽  
Content Type ◽  
Cultural Challenges ◽  
Intercultural Experience ◽  
Individual Spirituality

PurposeThe purpose of this qualitative study is to explore the role of spirituality, understood as a personal relationship with God, in missionary intercultural experience.Design/methodology/approachWe conducted narrative interviews with eight Polish consecrated missionaries in Argentina, Bolivia, Ecuador, Peru and Paraguay. We used thematic analysis to establish spirituality in missionary experience and narrative analysis to examine sensemaking processes.FindingsMissionary spirituality was defined by a personal relationship with God as a source of consolation, psychological comfort, strength to cope with distressing experiences, and Grace promoting self-improvement. It compensated for the lack of family and psychological support and enhanced psychological adjustment to the environment perceived as dangerous. Spirituality helped missionaries deal with cultural challenges, traumatic and life-threatening events. Traumatic experiences furthered their understanding of the mission and triggered a spiritual transition that entailed a change in their life, attitudes and behavior.Research limitations/implicationsComparative research into religious vs nonreligious individual spirituality in the experience across various types of expats in various locations could capture the professional and cultural specificity of individual spirituality. Research is also needed to link spirituality with expat failure.Practical implicationsCatholic agencies and institutions that dispatch missionaries to dangerous locations should consider providing professional psychological assistance. Narrative interviewing could be used to enhance missionaries' cultural and professional self-awareness, to better serve the local community. Their stories of intercultural encounters could be incorporated into cross-cultural training and the ethical and spiritual formation of students and future expats.Originality/valueThis study captures a spiritual aspect of intercultural experience of under-researched expats. It offers a model of the involvement of individual spirituality in coping in mission.

The Role of Wilms’ Tumor Protein 1 (WT1) in Chronic Myelogenous Leukemia Progression.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 4344-4344
Author(s):  
Liliana R. Souza ◽  
Luiz Penalva ◽  
Morgan McLemore
Keyword(s):  
Cellular Proliferation ◽  
Wilms Tumor ◽  
Blast Crisis ◽  
Regulatory Gene ◽  
Critical Role ◽  
Myelogenous Leukemia ◽  
Hematopoietic Progenitors ◽  
Hematopoietic Stem ◽  
Over Expression ◽  
Exact Function

Abstract Wilms’ tumor protein 1 (WT1) is a key regulatory gene whose function is required for proper development and differentiation of the heart, retina, genito-urinary, olfactory and hematopoietic systems. WT1 over expression is a characteristic observed in blasts of Chronic Myeloid Leukemia (CML). Despite this apparent co-relation, a direct link between WT1 over expression and progression of CML to blast crisis has never been established. The two major isoforms of WT1 are generated by alternative splicing; an event that involves the use of two alternative splice sites at the end of exon 9. The outcome is the insertion or not of only 3 amino acids (lysine, threonine and serine; KTS) at the c-terminus of the protein. The ratio between these isoforms is conserved among tissues and disruption of this balance can end up causing developmental abnormalities. Our group is testing if the over expression of WT1 in hematopoietic precursors can lead to progression in a murine model of CML. Over expression of WT1 has been shown to block differentiation in myeloid cell lines. However, over expression of WT1 in primary hematopoietic cells has variable effects on differentiation and proliferation. These differences may reflect differences in vector design and/or assays to evaluate. We have utilized the Murine Stem Cell Vector (MSCV), a retroviral construct that has high efficiency of transducing murine hematopoietic stem cells, to generated retroviral constructs. The vector contains an internal ribosomal entry site allowing expression of WT1+KTS or WT1-KTS isoforms with and without Bcr-Abl/GFP, the causative protein in CML. Retroviral transduction of hematopoietic progenitors was performed for 48 hours and FACS sorted GFP+Sca+lin− cells were utilized for colony forming assays in methylcellulose and in liquid culture. Our results have shown that primary murine hematopoietic progenitors transduced with Bcr-Abl/GFP and WT1+KTS demonstrate dramatically greater cytokine independent growth than progenitors transduced with Bcr-Abl/GFP alone. In contrast, progenitors transduced with Bcr-Abl/GFP and WT1-KTS demonstrate impaired growth. Figure Figure In addition transduced cells will be used to rescue lethally irradiated mice what will provide an excellent model to determine if WT1 cooperates with BCR-ABL to induce blast crisis. The exact function of each WT1 isoform is still unknown. However, there is clear data that they have different properties and are involved in different functions. Several lines of evidence suggest a role for the +KTS isoform in splicing. During hematopoietic cell differentiation, levels of WT1 protein were determined to decrease drastically. This fluctuation in WT1 expression level may play a critical role in the process of differentiation. In conclusion, our results strongly suggest that WT1 expression influences Bcr-Abl induced growth with the isoforms WT1+KTS and WT1-KTS displaying differential effects. Our data indicate that +KTS enhances Bcr-Abl dependent cellular proliferation while -KTS isoform impairs proliferation.

HLA class I-restricted lysis of leukemia cells by a CD8+ cytotoxic T-lymphocyte clone specific for WT1 peptide

Blood ◽  
2000 ◽  
Vol 95 (1) ◽  
pp. 286-293 ◽  
Author(s):  
Hideki Ohminami ◽  
Masaki Yasukawa ◽  
Shigeru Fujita
Keyword(s):  
T Lymphocyte ◽  
Bone Marrow Cells ◽  
Cytotoxic T Lymphocyte ◽  
Wilms Tumor ◽  
Myelogenous Leukemia ◽  
Leukemia Cells ◽  
Target Antigen ◽  
Human Leukemia ◽  
Wt1 Gene ◽  
Normal Cells

The Wilms tumor (WT1) gene has been reported to be preferentially expressed in acute leukemia cells, regardless of leukemia subtype and chronic myelogenous leukemia cells in blast crisis, but not in normal cells. This finding suggests strongly that WT1 protein is a potential target of immunotherapy for human leukemia. In this study, we established a CD8+ cytotoxic T-lymphocyte (CTL) clone directed against a WT1-derived peptide and examined its immunologic actions on leukemia cells. A CD8+ CTL clone, designated TAK-1, which lysed autologous cells loaded with a WT1-derived 9-mer peptide consisting of the HLA-A24 (HLA-A*2402)-binding motifs was established by stimulating CD8+ T lymphocytes from a healthy individual repeatedly with WT1 peptide-pulsed autologous dendritic cells. TAK-1 was cytotoxic to HLA-A24–positive leukemia cells expressing WT1, but not to HLA-A24–positive lymphoma cells that did not express WT1, HLA-A24–negative leukemia cells, or HLA-A24–positive normal cells. Treating leukemia cells with an antisense oligonucleotide complementary to the WT1 gene resulted in reduced TAK-1-mediated cytotoxicity, suggesting that target antigen of TAK-1 on leukemia cells is the naturally processed WT1 peptide in the context of HLA-A24. TAK-1 did not inhibit colony formation by normal bone marrow cells of HLA-A24–positive individuals. Because WT1 is overexpressed ubiquitously in various types of leukemia cells, but not in normal cells, immunotherapy using WT1 peptide-specific CTL clones should be an efficacious treatment for human leukemia. (Blood. 2000;95:286-293)

scholarly journals SYCHOEMOTIONAL CHARACTERISTICS OF THE ADOLESCENTS WITH ALLERGIC RHINITIS

2015 ◽  
Author(s):  
N. Adamia
Keyword(s):  
Allergic Rhinitis ◽  
World Population ◽  
Healthy Children ◽  
Psychological Profile ◽  
Symptom Checklist ◽  
Emotional Instability ◽  
Self Assessment ◽  
Research Results ◽  
Management Optimization ◽  
Psychological Features

Allergic rhinitis is a widespread allergic disease, with 35-40% prevalence in the world population. It is characterized with increasing frequency, particularly in children’s population. Aim:. Study of psycho-emotional profile in adolescents with allergic rhinitis of different severity. Materials and methods. Single-stage research was conducted, in compliance with the ethical norms. Study included 86 children (41% girls and 45% boys) of age from 11 to 13 years with allergic rhinitis of different severity and 30 healthy children. For the purpose of study of the patients’ psychological profile Esenek Personality Questionnaire (EPQ) intended for assessment of characterological and individual psychological features in children and adolescents (10-15 years) was used. Psycho-emotional sphere of the adolescents with allergic rhinitis was assessed also by Psychopathologic Symptom Checklist (Symptom Checklist-90-Revised-SCL-90-R). Clinical scale of self-assessment of psychical condition is widely applied in ambulatory and hospital practice. At the final stage of research the mathematical-statistical data processing was provided by means of SPSS/v12 software package. According to the research results, susceptibility to significant and mild introversion was identified in severe and average AR cases. Such patients are often locked into their inner world. These children are reserved, communicate with the parents and close friends only. They make decisions with care, love order, control their emotions, are pessimistic and rarely aggressive. Results of neuroticism study by G. Esenek techniques are provided in Table. Neuroticism is associated with the lability of nervous system, characterizes emotional condition or emotional lability (emotional stability or instability). Results of Symptom Checklist-90-Revised – SCL90R) are provided in the Table.Conclusion: according to the research results, allergic rhinitis is characterized with emotional instability, anxiety, as manifested by unsatisfactory adaptation, instable nature, depression, low resistance to the stress situations. Based on the conducted research, we regard that individual assessment of psychological profile of patients with allergic rhinitis would be reasonable, for the purpose of management optimization

scholarly journals PSYCHOLOGICAL FACTORS OF EMOTIONAL INSTABILITY IN ADOLESCENTS

2021 ◽  
Author(s):  
Gurak Halyna ◽  
Dolynska Lyubov
Keyword(s):  
Secondary School ◽  
Psychological Factors ◽  
Emotional Stability ◽  
Point Of View ◽  
Self Control ◽  
Practical Implementation ◽  
Emotional Instability ◽  
Emotional Life ◽  
Scientific Heritage ◽  
Empirical Point

The article presents an overview of the problem of the affective sphere of adolescents on both theoretical and empirical levels. Its relevance and, at the same time, the prospect for further research is justified. It was revealed that the emotional life of persons of pubertal period is differently disclosed in the scientific heritage of foreign scientists, but insufficiently covered in the domestic. Correlations between the emotional experiences of adolescents and their relationships with parents, peers; gender identity; eating disorders, etc., were established. Against the background of emphasizing the influence of various external psychological factors in the life of an adolescent, the topic of the intrapersonal, psychological potential of persons of this age remains open. Taking into account the topicality of the problem of emotional instability of teenagers, we aimed to find out in detail, from an empirical point of view, the internal psychological reasons of this phenomenon. For this purpose, the ascertaining experiment was carried out which involved 300 pupils of 6-7 and 8-9 forms of the general secondary educational institutions of the Ternopil region (Berezhanska Secondary School № 1 and Berezhanska Secondary School № 2). Respondents were offered specific, according to the formulated scientific problem, a package of psychodiagnostic techniques, directed to the research of features of the level of manifestation as well as categories of hypothetical factors of emotional stability of adolescents. The next stage of the study involved processing the data by factor analysis using the capabilities of Microsoft Excel and SPSS 21 software. As a result, 10 psychological factors that cause emotional instability in adolescents were identified. Among them: imbalance of self-esteem and personal abilities, personal insecurity compensated by self-control, low resistance to stress, impulsiveness against insecurity, dissonance in the assessment of one's own self, motivational polarity, emotional uncertainty, public insecurity, uncertainty in own abilities, confident behavior. The prospect of our study is the development and practical implementation of social psychological training as a means of correcting dysregulation of the affective sphere of personality and the development of their emotional stability.

scholarly journals Terapeutyczne aspekty wywiadu narracyjnego w perspektywie prowadzenia badań biograficznych

2018 ◽  
pp. 107-120
Author(s):  
Magda Karkowska
Keyword(s):  
Traumatic Experiences ◽  
Self Awareness ◽  
Narrative Interviews ◽  
The Past ◽  
Biographical Approach ◽  
Pedagogical Research

The article is devoted to an attempt to present a biographical approach in pedagogical research, taking into account not only exploratory but also therapeutic functions of narrative interviews. The reflections focus of looking for the answer to the question: whether and to what extent telling a story of one’s life (especially marked by traumatic experiences) may contribute to integrating the past and the present, increasing self-awareness, and thus (re-)constructing the narrator’s identity? I ask a question about the conditions to be met in order for the narrator to realize the limitations posed by the past and next to overcome the crisis by pointing out the main motives of action, epiphanies, crises, or breakthrough moments.

scholarly journals Compliance level in children with chronic pathology

2020 ◽  
Keyword(s):  
Diabetes Mellitus ◽  
Current Treatment ◽  
Control Group ◽  
Healthy Children ◽  
Emotional Instability ◽  
Daily Work ◽  
Idiopathic Juvenile Arthritis ◽  
Compliance Level ◽  
Compliance To Treatment ◽  
High Level

Introduction. Current treatment technologies, a significant place is occupied by the issue of interaction between a doctor and a patient. The aim of this study was to determine the level of compliance in children with chronic pathology. Materials and methods. 87 children aged 6 to 17 years were examined, among whom 32 children had idiopathic juvenile arthritis (JIA), 30 children had type 1 diabetes mellitus (DM1) and 25 apparently healthy children made up the control group. All children were interviewed using the Compliance Level Questionnaires for children with chronic pathology or healthy children. All statistical analyzes were performed using the s/n SPSS 17 4a 180844250981. Results. The study of compliance features in patients with IJA showed that the majority (84.4 %) of children had high compliance to treatment, which was much more common than in children with diabetes mellitus (р < 0,01) and control group (р < 0,02). Half of the children with DM1 also had a high level of general compliance. When assessing the structure of compliance in patients with diabetes, moderate levels of all components were found. Children of this group are characterized by an indefinite social position, they act according to the situation. Emotional instability is a typical manifestation. The study of the level of compliance in children of the control group revealed low indicators of general compliancy to the doctor's actions. Each component corresponded to the average level. It was in these children that low indicators of social and behavioral patterns were found, which indicates a negativity towards the treatment process and medical manipulations. Conclusions: 1. Patients with chronic pathology have a higher level of compliance than patients in the control group, which indicates a significant impact on the child's life of the presence of a chronic disease. 2. The level of compliance differs in various chronic diseases, may depend on the characteristics of subjective feelings and medical manipulations. 3. Female patients have a higher level of adherence to treatment, which dictates the need to take into account the gender of the child in the daily work of the doctor, and pay more attention to careful communication with boys.

Sign in / Sign up

Export Citation Format

Share Document