scholarly journals Biomimetic remineralization of acid etched enamel using agarose hydrogel model

F1000Research ◽  
2018 ◽  
Vol 7 ◽  
pp. 1476 ◽  
Author(s):  
Sara El Moshy ◽  
Marwa M.S. Abbass ◽  
Amal M. El-Motayam
Keyword(s):  
Phosphate Solution ◽  
Tooth Structure ◽  
Non Invasive ◽  
Minimally Invasive Dentistry ◽  
Demineralized Enamel ◽  
Agarose Hydrogel ◽  
Roughness Analysis ◽  
Acid Gel ◽  
The Stability ◽  
Mandibular Third Molars

Background: Minimally invasive dentistry aims to prevent progression of caries and treats non-cavitated lesions through non-invasive approaches to preserve the integrity of tooth structure. The aim of this research was to investigate the possible biomimetic effect of agarose hydrogel in remineralizing a human demineralized enamel model. Methods: Mandibular third molars were distributed into three groups (G1, G2 and G3) according to the follow up time (2, 4 and 6 days respectively). Caries like lesion was prepared by applying 37% phosphoric acid gel for 1 minute and then remineralization was performed through applying agarose hydrogel on the demineralized surfaces. The specimens were placed in phosphate solution at 37˚C for 2, 4 & 6 days. Scanning electron microscope (SEM), surface microhardness (SMH) and surface roughness analysis (SR) were performed to assess the regenerated tissue. Results: SEM revealed mineral depositions on the demineralized enamel surface that increased in density by time resulting in a relatively smooth surface in G3. SR and SMH analysis revealed significant differences between the remineralized enamel surfaces of different groups (p< 0.00001) with the highest SR in G1 and the highest SMH in G3. Conclusions: Agarose hydrogel application is a promising approach to treat early carious lesion. Further studies are needed to clarify the stability of agarose hydrogels in clinical application.

scholarly journals Mechanical and Electrical Performance of Flexible Polymer Film Designed for a Textile Electrically-Conductive Path

Materials ◽  
2021 ◽  
Vol 14 (9) ◽  
pp. 2169
Author(s):  
Agnieszka Tabaczyńska ◽  
Anna Dąbrowska ◽  
Marcin Masłowski ◽  
Anna Strąkowska
Keyword(s):  
Ionic Liquid ◽  
Ionic Liquids ◽  
Surface Resistance ◽  
Conductive Filler ◽  
Extrusion Process ◽  
Electrical Performance ◽  
Electrically Conductive ◽  
Non Invasive ◽  
Flexible Polymer ◽  
The Stability

Electro-conductive paths that are mechanically resistant and stable during simulated aging cycles are promising, in relation to the non-invasive application in e-textiles in our everyday surroundings. In the paper, an analysis of the influence of electro-conductive filler, as well as ionic liquid on surface resistance is provided. Authors proved that depending on the tested variant, obtained surface resistance may vary from 50 kΩ (when 50 phr of Ag and [bmim][PF6] ionic liquid applied) to 26 GΩ (when 25 phr of Ag and [bmim][PF6] ionic liquid applied). The samples were also evaluated after simulated aging cycles and the stability of electric properties was confirmed. Moreover, it was proved that the addition of ionic liquids reduced the resistance of vulcanizates, while no significant influence of the extrusion process on conductivity was observed.

scholarly journals A susceptibility locus on chromosome 13 profoundly impacts the stability of genomic imprinting in mouse pluripotent stem cells

2020 ◽  
Author(s):  
Emily Swanzey ◽  
Thomas F. McNamara ◽  
Effie Apostolou ◽  
Mamta Tahiliani ◽  
Matthias Stadtfeld
Keyword(s):  
Culture Conditions ◽  
Mouse Strains ◽  
Genetic Determinant ◽  
Pluripotent Cells ◽  
Developmental Potential ◽  
Chromosome 13 ◽  
Non Invasive ◽  
Epigenetic Instability ◽  
Trait Locus ◽  
The Stability

SummaryCultured pluripotent cells accumulate detrimental epigenetic alterations, including DNA methylation changes at imprinted genes known as loss-of-imprinting (LOI). Despite the substantial biomedical relevance of this phenomenon, the molecular cause of this epigenetic instability in pluripotent cells remains unknown. While the occurrence of LOI is generally considered a stochastic phenomenon, here we document a strong genetic determinant that segregates mouse pluripotent cells into epigenetically stable and unstable cell lines. Unstable lines exhibit hypermethylation at Dlk1-Dio3 and select other imprinted loci, which is associated with impaired developmental potential. Stimulation of demethylases by ascorbic acid prevents LOI and can preserve developmental potential. Susceptibility to LOI greatly differs between commonly used mouse strains, which we utilize to map a causal region on chromosome 13 with Quantitative Trait Locus (QTL) analysis. Our observations identify a strong genetic determinant of locus-specific epigenetic abnormalities in pluripotent cells and provide a non-invasive way to suppress them. This highlights the importance of considering genetics in conjunction with culture conditions for assuring the quality of pluripotent cells for biomedical applications.

scholarly journals Clinical Performance of a New Biomimetic Double Network Material

2013 ◽  
Vol 7 (1) ◽  
pp. 118-122 ◽  
Author(s):  
Christine Dirxen ◽  
Uwe Blunck ◽  
Saskia Preissner
Keyword(s):  
Soft Tissues ◽  
Clinical Performance ◽  
Young Patients ◽  
Tooth Wear ◽  
Clinical Situation ◽  
Dental Erosion ◽  
Thin Layers ◽  
Invasive Treatment ◽  
Tooth Structure ◽  
Non Invasive

Background:The development of ceramics during the last years was overwhelming. However, the focus was laid on the hardness and the strength of the restorative materials, resulting in high antagonistic tooth wear. This is critical for patients with bruxism.Objectives:The purpose of this study was to evaluate the clinical performance of the new double hybrid material for non-invasive treatment approaches.Material and Methods:The new approach of the material tested, was to modify ceramics to create a biomimetic material that has similar physical properties like dentin and enamel and is still as strong as conventional ceramics.Results:The produced crowns had a thickness ranging from 0.5 to 1.5 mm. To evaluate the clinical performance and durability of the crowns, the patient was examined half a year later. The crowns were still intact and soft tissues appeared healthy and this was achieved without any loss of tooth structure.Conclusions:The material can be milled to thin layers, but is still strong enough to prevent cracks which are stopped by the interpenetrating polymer within the network. Depending on the clinical situation, minimally- up to non-invasive restorations can be milled.Clinical Relevance:Dentistry aims in preservation of tooth structure. Patients suffering from loss of tooth structure (dental erosion, Amelogenesis imperfecta) or even young patients could benefit from minimally-invasive crowns. Due to a Vickers hardness between dentin and enamel, antagonistic tooth wear is very low. This might be interesting for treating patients with bruxism.

Conservative Anterior Partial Coverage CAD/CAM Restoration

2017 ◽  
Vol 42 (2) ◽  
pp. 117-121
Author(s):  
JT May
Keyword(s):  
Digital Technology ◽  
Computer Aided Design ◽  
Tooth Structure ◽  
Partial Coverage ◽  
Restorative Treatment ◽  
Minimally Invasive Dentistry ◽  
Computer Aided ◽  
Design And Manufacturing ◽  
Cad Cam ◽  
Aided Design

SUMMARY Computer-aided design and manufacturing technology enables practitioners to create, in a single appointment, indirect restorations that are esthetic and functionally unique to the patient's situation. The popular effort to perform minimally invasive dentistry using digital techniques with chairside milling can lead dentists to novel individualized restorative treatment. This article demonstrates a conservative anterior partial coverage restoration, utilizing both digital technology and chairside ceramic characterization to achieve an optimal esthetic outcome while preserving healthy tooth structure.

scholarly journals Non-Fluoridated Remineralising Agents - A Review of Literature

2021 ◽  
Vol 10 (9) ◽  
pp. 638-644
Author(s):  
Akriti Batra ◽  
Vabitha Shetty
Keyword(s):  
Dental Caries ◽  
Minimally Invasive ◽  
Medical Model ◽  
Acid Dissolution ◽  
Invasive Approach ◽  
Non Invasive ◽  
Minimally Invasive Dentistry ◽  
Phosphate Mineral ◽  
Modifiable Factors ◽  
Caries Management

Dental caries is not merely a continuous and one-way process of demineralisation of the mineral phase, but repeated episodes of demineralisations and remineralisation. The remineralisation process is a natural repair mechanism to restore the minerals again, in ionic forms, to the hydroxyapatite (HAP) crystal lattice. It occurs under nearneutral physiological pH conditions whereby calcium and phosphate mineral ions are redeposited within the caries lesion from saliva and plaque fluid resulting in the formation of newer HAP crystals, which are larger and more resistant to acid dissolution. An insight into the caries process’s multifactorial aetiopathogenesis has resulted in a paradigm shift towards minimally invasive dentistry. This era of personalised care using the medical model for caries management assimilates the signs of examining, diagnosing, intercepting, and managing dental caries at a microscopic level. Fluoride mediated salivary remineralisation system is considered the cornerstone of non-invasive approach for managing non-cavitated carious lesions. However, the effect of fluoride was found to be limited to the outer surface of the tooth, and it was observed that fluoride does not influence the modifiable factors in dental caries such as the biofilm. Hence, fluoride does not reduce the cariogenic challenge especially for high-risk individuals. Due to this gap in the conventional fluoride therapies, non-fluoridated remineralising systems have emerged, which interact with the tooth by delivering calcium and phosphorous ions, modifying the biofilm and neutralising the organic acids. This review re-examines the philosophy behind non-fluoridated remineralisation, their mode of action, availability in the market and evidence-based studies of the most promising advancements in remineralisation approaches. KEY WORDS Caries, Minimally Invasive Dentistry, Remineralisation, Non-Fluoridated Remineralising Systems

scholarly journals Non-Invasive Approaches for the Evaluation of the Functionalization of Melamine Foams with In-Situ Synthesized Silver Nanoparticles

Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 996 ◽  
Author(s):  
Suset Barroso-Solares ◽  
Paula Cimavilla-Roman ◽  
Miguel Angel Rodriguez-Perez ◽  
Javier Pinto
Keyword(s):  
Polymeric Nanocomposites ◽  
Free Standing ◽  
X Ray ◽  
Color Changes ◽  
Non Invasive ◽  
Silver Nps ◽  
Promising Solution ◽  
The Stability

The use of polymeric nanocomposites has arisen as a promising solution to take advantage of the properties of nanoparticles (NPs) in diverse applications (e.g., water treatment, catalysis), while overcoming the drawbacks of free-standing nanoparticles (e.g., aggregation or accidental release). In most of the cases, the amount and size of the NPs will affect the stability of the composite as well as their performance. Therefore, a detailed characterization of the NPs present on the nanocomposites, including their quantification, is of vital importance for the optimization of these systems. However, the determination of the NPs load is often carried out by destructive techniques such as TGA or ICP-OES, the development of non-invasive approaches to that aim being necessary. In this work, the amount of silver NPs synthesized directly on the surface of melamine (ME) foams is studied using two non-invasive approaches: colorimetry and X-ray radiography. The obtained results show that the amount of silver NPs can be successfully determined from the luminosity and global color changes of the surface of the foams, as well as from the X-ray attenuance.

Remineralization of Natural Tooth Enamel in Artificial Saliva Environment

2017 ◽  
Vol 68 (3) ◽  
pp. 510-514 ◽  
Author(s):  
Agripina Zaharia ◽  
Viorica Ghisman Plescan ◽  
Irina Atkinson ◽  
Oana Catalina Mocioiu ◽  
Alina Cantaragiu ◽  
...  
Keyword(s):  
Tooth Enamel ◽  
Artificial Saliva ◽  
Dental Enamel ◽  
Hexagonal Structure ◽  
Enamel Surface ◽  
Enamel Matrix Derivative ◽  
Phosphate Solution ◽  
X Ray ◽  
Composition And Structure ◽  
Demineralized Enamel

This paper investigates in situ remineralization of the acid-etched natural enamel surface, by incubation in a phosphate solution with neutral pH, at 37 �C for 4-10 days, without using any enamel matrix derivative (EMD). We investigated the morphology, crystallinity, chemical composition and structure of the newly grown layer onto the natural enamel surface stored in contact with artificial saliva (AS) having a composition similar to natural oral environment. The crystalline phases, crystallite size and orientation, as well as the chemical and phase composition of the remineralized dental enamel samples were studied by scanning electron microscopy coupled with energy dispersive X-ray spectrometry, Fourier transform infrared spectroscopy and X-ray diffraction. The experimental results showed that the enamel crystals grown on the demineralized enamel surface are mainly Ca-deficient apatite hexagonal structure. Fluoridated hydroxyapatite and sylvite-type KCl crystallites, which amount considerable increased in the case of sample stored in AS for 10 days, were also identified.

The Role of Dynamic Magnetic Resonance Imaging in the Detection of Lesions of the Ulnocarpal Complex

1996 ◽  
Vol 21 (3) ◽  
pp. 311-314 ◽  
Author(s):  
M. Gabl ◽  
M. Lener ◽  
S. Pechlaner ◽  
W. Judmaier
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Dynamic Mri ◽  
Dynamic Magnetic Resonance Imaging ◽  
Resonance Imaging ◽  
Non Invasive ◽  
Dynamic Magnetic Resonance ◽  
Magnetic Resonance Imaging Mri ◽  
The Stability ◽  
A Prospective Study

A prospective study of 32 patients was carried out to investigate the significance of dynamic magnetic resonance imaging (MRI) in diagnosis of triangular fibrocartilage (TFC) lesions. Tears of the TFC can be diagnosed well by means of static MRI and arthroscopy. Dynamic MRI examination has an advantage in evaluating the stability of the TFC and ulnocarpal impingement. By means of dynamic MRI it was possible to make a preoperative diagnosis of an ulnocarpal impingement in five patients, a diagnosis which was confirmed through arthroscopy in all cases. In three further patients, dynamic MRI showed ulnocarpal impingement caused by instability of the ulnar attachment of the TFC. This kind of impingement could not be ascertained arthroscopically. Dynamic MRI extends the possibilities of evaluating obscure ulnar wrist pain. Its significance lies in the non-invasive examination of ulnocarpal impingement as well as the evaluation of TFC stability.

scholarly journals Application of GPR Method for Detection of Loose Zones in Flood Levee

2018 ◽  
Vol 30 ◽  
pp. 01008 ◽  
Author(s):  
Tomisław Gołębiowski ◽  
Tomasz Małysa
Keyword(s):  
Signal Processing ◽  
Important Influence ◽  
Water Filtration ◽  
Three Solutions ◽  
Non Invasive ◽  
Vistula River ◽  
Standard Signal ◽  
River Flood ◽  
The Stability ◽  
The Village

In the paper the results of non-invasive georadar (GPR) surveys carried out for detection of loose zones located in the flood levee was presented. Terrain measurements were performed on the Vistula river flood levee in the village of Wawrzeńczyce near Cracow. In the investigation site, during the flood in 2010, leakages of levee were observed, so detection of inner water filtration paths was an important matter taking into account the stability of the levee during the next flood. GPR surveys had reconnaissance character, so they were carried out with the use of short-offset reflection profiling (SORP) technique and radargrams were subjected to standard signal processing. The results of surveys allowed to outline main loose zone in the levee which were the reason of leakages in 2010. Additionally gravel interbeddings in sand were detected which had an important influence, due to higher porosity of such zones, to water filtration inside of the levee. In the paper three solutions which allow to increase quality and resolution of radargrams were presented, i.e. changeable-polarisation surveys, advanced signal processing and DHA procedure.

scholarly journals VERIFICATION OF CLINICAL ACCURACY OF AUTOMATED NON-INVASIVE SPHYGMOMANOMETERS: IS IT APPROPRIATE TO USE BLOOD PRESSURE SIMULATORS?

2020 ◽  
Vol 50 (1) ◽  
pp. 5-11
Author(s):  
Jan Havlík ◽  
Vratislav Fabián
Keyword(s):  
Blood Pressure ◽  
Developed Countries ◽  
Point Of View ◽  
Blood Pressure Monitor ◽  
Pressure Monitor ◽  
Non Invasive ◽  
Precise Knowledge ◽  
Wilcoxon Rank Sum Test ◽  
Clinical Accuracy ◽  
The Stability

Cardiovascular disease is the most common cause of death in developed countries. Blood measurement is an integral part of the diagnosis of these diseases. With the development of oscillometric blood pressure monitors, the question of regular monitoring of their clinical accuracy (overall error) has arisen. This paper deals with the overall accuracy of two commercial tonometers (Hartmann Digital HG 160 comfort and HuBDIC HBP–1520), using two calibrated blood pressure simulators (Fluke BP Pump 2 and Fluke ProSim). Using the Wilcoxon rank-sum test, significant differences between the simulators have been proved for all measurements – both for SBP and DBP measurements and both for Hartmann Digital HG 160 and HuBDIC HBP–1520 tonometers (p < 0.001). Therefore, without the precise knowledge of the relationship between the blood pressure monitor and the simulator used, it is not appropriate to use simulators to determine the overall error. On the other hand, the tested devices had a very good repeatability of the measurements at all presets, with both simulators. From this point of view, it is suitable to use simulators to determine the stability of measurement by a given tonometer rather than its clinical accuracy.

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