scholarly journals Safety, efficacy and utility of methods of transferring adhesive and cohesive Escherichia coli cells to microplates to avoid aerosols

F1000Research ◽  
2014 ◽  
Vol 3 ◽  
pp. 267 ◽  
Author(s):  
Bryan Ericksen
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Data Analysis ◽  
Antimicrobial Agents ◽  
Enterobacter Aerogenes ◽  
Cross Contamination ◽  
Escherichia Coli Cells ◽  
Threshold Time ◽  
Biosafety Level

The virtual colony count (VCC) microbiological assay has been utilized for over a decade to measure the antimicrobial activity of peptides such as defensins and LL-37 against biosafety level (BSL)-1 and BSL-2 bacteria including Escherichia coli, Staphylococcus aureus, Bacillus cereus, and Enterobacter aerogenes.  In addition, a modified pipetting technique was presented in a 2011 study of defensin activity against the BSL-3 pathogen Bacillus anthracis.  Both studies were published in the journal Antimicrobial Agents and Chemotherapy.  Here we report that the method can also detect cross-contamination caused by aerosols utilizing the VCC method of data analysis by quantitative growth kinetics (QGK).  The QGK threshold time, or Tt, equivalent to the cycle time Ct reported in 1996 by Heid et al., precisely identifies when wells were inoculated.

scholarly journals Safety, efficacy and utility of methods of transferring adhesive and cohesive Escherichia coli cells to microplates to avoid aerosols

F1000Research ◽  
2015 ◽  
Vol 3 ◽  
pp. 267 ◽  
Author(s):  
Bryan Ericksen
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Data Analysis ◽  
Antimicrobial Agents ◽  
Enterobacter Aerogenes ◽  
Cross Contamination ◽  
Escherichia Coli Cells ◽  
Threshold Time ◽  
Biosafety Level

The virtual colony count (VCC) microbiological assay has been utilized for over a decade to measure the antimicrobial activity of peptides such as defensins and LL-37 against biosafety level (BSL)-1 and BSL-2 bacteria including Escherichia coli, Staphylococcus aureus, Bacillus cereus, and Enterobacter aerogenes.  In addition, a modified pipetting technique was presented in a 2011 study of defensin activity against the BSL-3 pathogen Bacillus anthracis.  Both studies were published in the journal Antimicrobial Agents and Chemotherapy.  Here I report that the method can also detect cross-contamination caused by aerosols utilizing the VCC method of data analysis by quantitative growth kinetics (QGK).  The QGK threshold time, or Tt, equivalent to the cycle time Ct reported in 1996 by Heid et al., precisely identifies when wells were inoculated.

scholarly journals Synthesis and biological evaluation of some new 7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazines as antimicrobial agents

2022 ◽  
Vol 11 (1) ◽  
pp. 75-82 ◽  
Author(s):  
Iryna Myrko ◽  
Taras Chaban ◽  
Vasyl Matiychuk
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Biological Evaluation ◽  
Bacterial Strains ◽  
Synthesis And Biological Evaluation

A series of some new pyrazole-substituted 7H-[1,2,4]triazolo[3,4-b][1,3,4]thiadiazines was synthesized in this study. The structures of target substances were confirmed by using 1H and 13С NMR spectroscopy, mass spectrometry and elemental analysis. The synthesized compounds have been evaluated for antimicrobial activity against five bacterial strains (Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Staphylococcus aureus) and two fungal strains (Candida albicans and Cryptococcus neoformans). The antimicrobial screening studies of synthesized substances established that 2 of 12 compounds show pronounced antibacterial activity against the strain Staphylococcus aureus.

scholarly journals In-vitro antibacterial activity of sequential crude extracts from Datura stramonium seeds

2016 ◽  
Vol 1 (1) ◽  
pp. 1-4
Author(s):  
Powar Priyatama V ◽  
Powar Trupti A
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Agents ◽  
Datura Stramonium ◽  
Seed Extract ◽  
Zone Of Inhibition

The growing phenomenon of antibiotic resistance, particularly to pathogenic microorganisms, in current medicine, has directed the concern of scientists for finding novel antimicrobial agents from plant origin with negligible side effect. The present study was aimed to phytochemical investigation and antimicrobial activity of seed extract of Datura stramonium in sequentially with different organic solvents. For this, antimicrobial properties were tested against bacteria Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia and Bacillus subtilis by cup plate method. Among the tested bacterial Klebsiella pneumoniae was the most inhibited majorly with the chloroform extract. Datura stramonium chloroform seed extract produced maximum zone of inhibition 26 mm against Klebsiella pneumoniae and 12 mm against Bacillus subtilis and 13 mm against Escherichia coli. Datura Stramonium methanol seed extract produced maximum zone of inhibition 27 mm against Pseudomonas aeruginosa and 15 mm against Bacillus subtilis, 14 mm against Staphylococcus aureus and 19 mm against Escherichia coli. Datura stramonium petroleum ether seed extract produced 16 mm zone of inhibition against Escherichia coli. Datura stramonium aqueous seed extract exhibits 24 mm zone of inhibition against Bacillus subtilis. All the experienced solvent extracts showed potential antimicrobial activity Index against various tested microorganisms. Owning to the results, it can be concluded that the extracts of the Datura stramonium can be used to design different herbal antimicrobial agents.

scholarly journals Synthesis and Evaluation of Some New Thiazolidin-4-One Derivatives as Potential Antimicrobial Agents

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
B. A. Baviskar ◽  
S. S. Khadabadi ◽  
S. L. Deore
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Salmonella Typhi ◽  
Zone Of Inhibition ◽  
Structure Activity

A new series ofN-{4-methyl-5-[4-(4-oxo-2-phenyl(1,3-thiazolidin-3-yl)]-5-sulfanyl(1,2,4-triazol-3-yl)-1,3-thiazol-2-yl }acetamide (7a-l) was synthesized in order to determine their antimicrobial activity and feasible structure–activity relationships. The compounds were synthesized in good yield and the structures of all newly synthesized compounds were established on the basis of their IR,1HNMR, and elemental analysis. The synthesized compounds were testedin vitroantibacterial activity againstStaphylococcus aureus,Escherichia coli,Pseudomonas aeruginosaandSalmonella typhiand antifungal activity againstAspergillus niger,Candida albicansby measuring the zone of inhibition in mm.

scholarly journals Antimicrobial Activity and Chemical Composition of Callistemon Comboynensis and C. Citrinus Leaf Essential Oils from the Northern Plains of India

2008 ◽  
Vol 3 (11) ◽  
pp. 1934578X0800301
Author(s):  
Shikha Gupta ◽  
Ajai Kumar ◽  
Kunal Srivastava ◽  
Santosh K. Srivastava ◽  
Suaib luqman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Essential Oils ◽  
Antimicrobial Agents ◽  
Trichophyton Rubrum ◽  
Sporothrix Schenckii ◽  
Herbal Medicines ◽  
Enterobacter Aerogenes ◽  
Northern Plains ◽  
Broth Dilution

Biological and chemical investigations were carried out to evaluate the antimicrobial potential of Callistemon comboynensis and C. citrinus leaf oils for herbal medicines. Disk diffusion and micro broth dilution methods were used for the evaluation of the antimicrobial activity of the essential oils against selected strains of bacteria (Staphylococcus aureus, S. epidermidis, Streptococcus mutans, Enterococcus faecalis, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterobacter aerogenes and Escherichia coli) and fungi (Candida albicans, Cryptococcus neoformans, Trichophyton rubrum, Aspergillus flavus, A. niger and Sporothrix schenckii). Analysis of the leaf essential oils of C. comboynensis and C. citrinus using GC and GC-MS resulted in the identification of thirty and twenty eight compounds, respectively. 1,8-Cineole (58.5% and 58.0%), α-pinene (22.7% and 24.1%), and (E)-β-terpineol (6.4% and 1.8%), were the major constituents. The isolated essential oils could be of use for the future development of antimicrobial agents.

Synthesis of s-Triazine-Based Thiazolidinones as Antimicrobial Agents

2012 ◽  
Vol 67 (3-4) ◽  
pp. 108-122
Author(s):  
Divyesh Patel ◽  
Rahul Patel ◽  
Premlata Kumari ◽  
Navin B. Patel
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Shigella Flexneri ◽  
Salmonella Typhi ◽  
Proteus Vulgaris ◽  
Aspergillus Clavatus ◽  
Schiff Base Derivatives

5A novel series of thiazolidinone derivatives, namely 4-{4-dimethylamino-6-[4-oxo- 2-phenyl-5-(4-pyridin-2-yl-piperazin-1-ylmethyl)-thiazolidin-3-yl]-[1,3,5]-triazin-2-yloxy}- 1-methyl-1H-quinolin-2-ones, have been synthesized from the key intermediate 4-(4-amino- 6-dimethylamino-[1,3,5]-triazin-2-yloxy)-1-methyl-1H-quinolin-2-one (). Compound 5 was condensed with various aldehydes to give Schiff base derivatives, which after cyclization gave thiazolidinones that were linked with 1-pyridin-2-yl-piperazine to obtain the target compounds. The newly synthesized compounds were evaluated for their antimicrobial activity against eight bacteria (Staphylococcus aureus, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella typhi, Proteus vulgaris, Shigella flexneri) and four fungi (Aspergillus niger, Candida albicans, Aspergillus fumigatus, Aspergillus clavatus)

Antimicrobial Activity of Bidens pilosa Leaves Extracts Against Staphylococcus aureus and Escherichia coli

2021 ◽  
Vol 38 ◽  
pp. 20-24
Author(s):  
S.A. Mohamed ◽  
C. Mathew
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antimicrobial Agents ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Bidens Pilosa ◽  
Leaf Extracts ◽  
E Coli ◽  
Tract Infections

Resistance against synthetic antimicrobial agents is one of the major global public health challenges that compel scientists to search for alternatives including those of plants origin. Staphylococcus aureus and Escherichia coli are bacteria responsible for a variety of infections and diseases that causes significant morbidity and mortality in humans and animals. E. coli is widely distributed in nature and commonly found in lower gastro intestinal tracts of most warm-blooded animals associated with urinary tract infections and enterocolitis in humans and colibacillosis in poultry. This study was carried out to investigate antimicrobial activity of methanolic leaf extracts of Bidens pilosa against S. aureus and E. coli. Agar well diffusion method was used to assess antimicrobial activity of the leaf extracts at 20%, 50% and 70% concentrations respectively based on measured zone of inhibition. The leaf extracts of Bidens pilosa produced significant zone of inhibition indicating its antimicrobial activity against E. coli and S. aureus. The antimicrobial activity was demonstrated in all concentrations however, the highest zone of inhibition (18.5mm and 32mm) for E. coli and S. aureus respectively was at 70% concentration. The results shows that Bidens pilosa leaf extracts have antimicrobial activity against the tested bacteria and have the potential for further development including identification of active components that can be tested for treatment of E. coli and S. aureus associated conditions.

scholarly journals Antimicrobial activity of different substituted meso-porphyrin derivatives

2020 ◽  
Vol 28 (2) ◽  
pp. 205-216 ◽  
Author(s):  
Luminița Sălăgeanu ◽  
Delia Muntean ◽  
Horhat Florin George ◽  
Anca Lascu ◽  
Diana Anghel ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Streptococcus Pyogenes ◽  
Antimicrobial Agents ◽  
Water Soluble ◽  
Diffusion Method ◽  
Dilution Method ◽  
Moderate Activity ◽  
Porphyrin Derivatives

AbstractThe increasing resistance against classical antibiotic treatment forces the researchers to develop novel non-toxic antimicrobial agents. The aim of this study was to determine the antimicrobial properties of seven different porphyrins having distinctive hydrophobicity/hydrophilicity: P1 meso-tetra(4-methoxy-phenyl)porphyrin, P2 Zn(II)-meso-5,10,15,20-tetrapyridylporphyrin, P3 meso-tetra(p-tolyl)porphyrin, P4 5,10,15,20-tetraphenylporphyrin; P5 (5,10,15,20-tetraphenylporphinato) dichlorophosphorus(V) chloride, P6 5,10,15,20-tetrakis-(N-methyl-4-pyridyl) porphyrin-Zn(II) tetrachloride, P7 Zn(II)-5,10,15,20-meso-tetrakis-(4-aminophenyl)porphyrin. The meso-porphyrin derivatives were screened for their antimicrobial activity against six reference strains: Streptococcus pyogenes ATCC 19615, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, Pseudomonas aeruginosa ATCC27853 and Candida albicans ATCC 10231. The antimicrobial activity of these samples was evaluated by the agar disk diffusion method and dilution method, with the determination of the minimum inhibitory concentration (MIC), the minimum bactericidal concentration (MBC) and the minimum fungicidal concentration (MFC). The most significant result is provided by the water-soluble P5 manifesting an obvious antimicrobial activity against Streptococcus pyogenes. On the other hand, P6 is a moderately active derivative against Streptococcus pyogenes and Escherichia coli and P7 presents moderate activity against Streptococcus pyogenes and Staphylococcus aureus. All the tested porphyrin bases, presenting hydrophobic character, have no antimicrobial activity under the investigated conditions. The common characteristics of the porphyrins that act as promising antimicrobial agents in the non-irradiated methods are: the cationic nature, the increased hydrophilicity and the presence of both amino functional groups grafted on the porphyrin ring and the coordination with Zn or phosphorus in the inner core.

Screening and antimicrobial activity of actinomycetes isolated from the rhizosphere of Clitoria ternatea

2016 ◽  
Vol 1 (01) ◽  
Author(s):  
Vemavarapu Bhaskara Rao ◽  
Kandlagunta Guru Prasad ◽  
Krishna Naragani ◽  
Vijayalakshmi Muvva
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Calcium Carbonate ◽  
Rhizosphere Soil ◽  
Soil Samples ◽  
Antimicrobial Spectrum ◽  
Clitoria Ternatea ◽  
Primary Screening

The air dried rhizosphere soil samples pretreated with calcium carbonate was employed for the isolation of actinomycete strains. Serial dilution plate technique was used for the isolation of actinomycetes. A total of 20 actinomycete strains designated as BS1-BS20 were isolated from the rhizosphere of medicinal plant Clitoria ternatea. All the 20 strains were subjected to primary screening for antimicrobial activity. Among the 20 strains screened, 10 strains exhibited high antimicrobial spectrum against Staphylococcus aureus, Escherichia coli and Candida albicans.

Synthesis, photosensitization and antimicrobial activity evaluation of some novel Merocyanine dyes

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Aspergillus Flavus ◽  
Elemental Analysis ◽  
Cyanine Dyes ◽  
Fungal Strains ◽  
Activity Evaluation ◽  
Visible Spectra ◽  
Merocyanine Dyes

Novel acyclic and cyclic merocyanine dyes derived from the nucleu of furo [(3,2-d) pyrazole; ( d 2 , 3 )imidazole]were prepared. The electronic visible absorptionspectra of all the synthesized new cyanine dyes were examined in 95% ethanolsolution to evaluate their photosensitization properties. Antibacterial andantifungal activities for some selected dyes were tested against various bacterialand fungal strains (Escherichia coli, Staphylococcus aureus, Aspergillus flavus andCandida albicans) to evaluate their antimicrobial activity. Structural identificationwas carried out via elemental analysis, visible spectra, IR and 1H NMRspectroscopic data.

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