scholarly journals Ecologically Safe Cavity Way of Hydrogen Generation in Water Flows with Low Ionized Plasma Emergence

10.12737/1574 ◽  
2013 ◽  
Vol 2 (5) ◽  
pp. 21-24
Author(s):  
Якушин ◽  
R. Yakushin ◽  
Нурахметов ◽  
T. Nurakhmetov ◽  
Кутербеков ◽  
...  
Keyword(s):  
Line Intensity ◽  
Atomic Hydrogen ◽  
Hydrogen Generation ◽  
Safe Method ◽  
Radiation Line ◽  
Water Flows ◽  
A Cell ◽  
High Degree

Observed changes of atomic hydrogen radiation line intensity in the spectrum for plasma formations arising at a collapse of cavity bubbles in the water of high degree cleaning have been described. The constructive scheme of a cell for cavity creation with subsequent ecologically safe method of hydrogen concoction from a gas mix has been offered. The influence of cavity creation device material and chemicals impurity on the spectrum for hydrogen’s luminescence brightness and its formation speed has been experimentally established. The results of experiments confirm the possibility of hydrogen safe generation in cavity streams and make actual the need for further researches.

Nonessential-Nonhub Proteins in the Protein-Protein Interaction Network

2014 ◽  
Vol 934 ◽  
pp. 159-164
Author(s):  
Yun Yuan Dong ◽  
Xian Chun Zhang
Keyword(s):  
Protein Interaction ◽  
Interaction Network ◽  
Clustering Coefficient ◽  
Centrality Measures ◽  
Ppi Network ◽  
Protein Protein Interaction ◽  
Ppi Networks ◽  
Comparison Results ◽  
A Cell ◽  
High Degree

Protein-protein interaction (PPI) networks provide a simplified overview of the web of interactions that take place inside a cell. According to the centrality-lethality rule, hub proteins (proteins with high degree) tend to be essential in the PPI network. Moreover, there are also many low degree proteins in the PPI network, but they have different lethality. Some of them are essential proteins (essential-nonhub proteins), and the others are not (nonessential-nonhub proteins). In order to explain why nonessential-nonhub proteins don’t have essentiality, we propose a new measure n-iep (the number of essential neighbors) and compare nonessential-nonhub proteins with essential-nonhub proteins from topological, evolutionary and functional view. The comparison results show that there are statistical differences between nonessential-nonhub proteins and essential-nonhub proteins in centrality measures, clustering coefficient, evolutionary rate and the number of essential neighbors. These are reasons why nonessential-nonhub proteins don’t have lethality.

scholarly journals Chloromethane-Induced Genes Define a Third C1 Utilization Pathway in Methylobacterium chloromethanicum CM4

2002 ◽  
Vol 184 (13) ◽  
pp. 3476-3484 ◽  
Author(s):  
Alex Studer ◽  
Craig McAnulla ◽  
Rainer Büchele ◽  
Thomas Leisinger ◽  
Stéphane Vuilleumier
Keyword(s):  
Dna Sequences ◽  
Reductase Activity ◽  
Cell Extract ◽  
Pcr Analysis ◽  
A Cell ◽  
Folate Hydrolase ◽  
Specific Oxidation ◽  
Transcriptional Start Sites ◽  
Utilization Pathway ◽  
High Degree

ABSTRACT Methylobacterium chloromethanicum CM4 is an aerobic α-proteobacterium capable of growth with chloromethane as the sole carbon and energy source. Two proteins, CmuA and CmuB, were previously purified and shown to catalyze the dehalogenation of chloromethane and the vitamin B12-mediated transfer of the methyl group of chloromethane to tetrahydrofolate. Three genes located near cmuA and cmuB, designated metF, folD and purU and encoding homologs of methylene tetrahydrofolate (methylene-H4folate) reductase, methylene-H4folate dehydrogenase-methenyl-H4folate cyclohydrolase and formyl-H4folate hydrolase, respectively, suggested the existence of a chloromethane-specific oxidation pathway from methyl-tetrahydrofolate to formate in strain CM4. Hybridization and PCR analysis indicated that these genes were absent in Methylobacterium extorquens AM1, which is unable to grow with chloromethane. Studies with transcriptional xylE fusions demonstrated the chloromethane-dependent expression of these genes. Transcriptional start sites were mapped by primer extension and allowed to define three transcriptional units, each likely comprising several genes, that were specifically expressed during growth of strain CM4 with chloromethane. The DNA sequences of the deduced promoters display a high degree of sequence conservation but differ from the Methylobacterium promoters described thus far. As shown previously for purU, inactivation of the metF gene resulted in a CM4 mutant unable to grow with chloromethane. Methylene-H4folate reductase activity was detected in a cell extract of strain CM4 only in the presence of chloromethane but not in the metF mutant. Taken together, these data provide evidence that M. chloromethanicum CM4 requires a specific set of tetrahydrofolate-dependent enzymes for growth with chloromethane.

scholarly journals CellS: A Cell-Inspired Efficient Software Framework for AI-Enabled Application on Resources-Constrained Mobile System

Electronics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 568
Author(s):  
Ching-Han Chen ◽  
Mu-Che Wu
Keyword(s):  
Software Development ◽  
Intelligent System ◽  
Cell System ◽  
Software Framework ◽  
Multiple Cores ◽  
A Cell ◽  
Computing Performance ◽  
Mobile Processor ◽  
High Degree ◽  
Efficient Software

Today’s mobile processors generally have multiple cores and sufficient hardware resources to support AI-enabled software operation. However, very few AI applications make full use of the computing performance of mobile multiprocessors. This is because the typical software development is sequential, and the degree of parallelism of the program is very low. In the increasingly complex AI-driven and software development projects with natural human–computer interaction, this will undoubtedly cause a waste of mobile computing resources that are originally limited. This paper proposes an intelligent system software framework, CellS, to improve smart software development on multicore mobile processor systems. This software framework mimics the cell system. In this framework, each cell can autonomously aware changes in the environment (input) and reaction (output) and may change the behavior of other cells. Smart software can be regarded as a large number of cells interacting with each other. Software developed based on the CellS framework has a high degree of scalability and flexibility and can more fully use multicore computing resources to achieve higher computing efficiency.

scholarly journals Roles for the RNA polymerase III regulator MAFR-1 in regulating sperm quality in Caenorhabditis elegans

2020 ◽  
Author(s):  
Amy M. Hammerquist ◽  
Sean P. Curran
Keyword(s):  
Caenorhabditis Elegans ◽  
Rna Polymerase ◽  
Male Fertility ◽  
Sperm Quality ◽  
Rna Polymerase Iii ◽  
Transcript Abundance ◽  
Negative Regulator ◽  
A Cell ◽  
Pol Iii ◽  
High Degree

ABSTRACTThe negative regulator of RNA polymerase (pol) III mafr-1 has been shown to affect RNA pol III transcript abundance, lipid biosynthesis and storage, progeny output, and lifespan. We deleted mafr-1 from the Caenorhabditis elegans genome and found that animals lacking mafr-1 replicated many phenotypes from previous RNAi-based studies, but strikingly not the oocyte-related reproductive phenotypes. Utilizing a yeast two-hybrid assay, we discovered several novel interactors of MAFR-1 that are expressed in a sperm- and germline-enriched manner. In support of a role for MAFR-1 in the male germline, we found mafr-1 null males have smaller spermatids that are less capable in competition for fertilization; a phenotype that was dependent on RNA pol III activity. Restoration of MAFR-1 expression specifically in the germline rescued the spermatid-related phenotypes, suggesting a cell autonomous role for MAFR-1 in nematode male fertility. Based on the high degree of conservation of Maf1 activity across species, our study may inform similar roles for Maf1 and RNA pol III in mammalian male fertility.

scholarly journals A Monoclonal Antibody Specific to Surface Antigen on Candida krusei

1998 ◽  
Vol 5 (1) ◽  
pp. 121-124
Author(s):  
Raymond Robert ◽  
Odile Faure ◽  
Arnaud Carloti ◽  
Bernadette Lebeau ◽  
Christian Bernard ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Fungal Infections ◽  
Immunofluorescence Assay ◽  
Candida Krusei ◽  
Tissue Samples ◽  
A Cell ◽  
Fluconazole Resistant ◽  
Resistant Strains ◽  
High Degree ◽  
Protein Moiety

ABSTRACT A monoclonal antibody (MAb; MAb 6B3) which reacts specifically with a cell wall antigen found in all strains or isolates of Candida krusei was developed. MAb 6B3 was extensively tested by immunofluorescence assay for cross-reaction with manyCandida, Cryptococcus,Saccharomyces, Trichosporon, andRhodotorula species and was found to react only with the species C. krusei. The specific epitope is expressed on the surface of fungal cells and appears to reside on a protein moiety. Taking into account the increasing importance of fluconazole-resistant strains in nosocomial fungal infections, the very high degree of specificity of this MAb for C. krusei could be useful for the routine detection of C. krusei in culture or in tissue samples.

Probing the spatial organization of nucleic acids within cells by nonisotopic in situ hybridization

1992 ◽  
Vol 50 (1) ◽  
pp. 10-11
Author(s):  
Jeanne Bentley Lawrence
Keyword(s):  
In Situ Hybridization ◽  
Nucleic Acids ◽  
Spatial Organization ◽  
Experimental Approach ◽  
Single Cells ◽  
Potential Applications ◽  
A Cell ◽  
Differential Gene ◽  
High Degree

In situ hybridization is a powerful experimental approach that directly couples molecular and cytological information in a visual context. Advances in hybridization procedures over recent years, coupled with previously described non-isotopic labelling methods developed in a number of laboratories, now provide a way to detect nucleic acids within cells with a high degree of resolution and sensitivity. Adaptations of this technology allow either DNA or RNA to be detected and visualized either with the light microscope, using fluorescence or colorimetric methods, or with the electron microscope using antibodies conjugated to gold or peroxidase. The potential applications of this technology are relevant to numerous areas of biomedical research and range from the more straightforward study of differential gene expression in single cells within a population to the precise localization of individual genes or RNAs within the cytoplasm or nucleus of a cell.

The biophysics of ligand–receptor interactions

1980 ◽  
Vol 13 (2) ◽  
pp. 201-230 ◽  
Author(s):  
Charles DeLisi
Keyword(s):  
Biological Activity ◽  
Plasma Membrane ◽  
Cell Surface ◽  
Background Noise ◽  
Information Transfer ◽  
Receptor Interactions ◽  
A Cell ◽  
Cell Surface Glycoproteins ◽  
High Degree ◽  
To Receive

For the cells of an organism to act in the coordinated fashion necessary for complex functioning, they must be able to receive and transmit information. Information transfer is mediated by molecules released by the cells and may be local, as in the case of neurotransmitters, or long range, as in the case of hormones. It is apparent, however, that irrespective of the range of interaction, a cell must be able to distinguish, with a high degree of precision, the signals relevant to it from an enormous flow of background noise.Molecular recognition at the cell surface is mediated by receptors: cell surface glycoproteins that usually form an integral part of the plasma membrane (see, for example, Cuatrecasas & Greaves, 1978). Typically, receptors bind the ligands they are designed to recognize with affinities of the order of 108 M-1, and they translate that interaction into a sequence of signals that ultimately lead to biological activity.

Effects of H2O on Atomic Hydrogen Generation in Hydrogen Plasma

1993 ◽  
Vol 32 (Part 1, No. 6B) ◽  
pp. 3120-3124 ◽  
Author(s):  
Jun Kikuchi ◽  
Shuzo Fujimura ◽  
Masafumi Suzuki ◽  
Hiroshi Yano
Keyword(s):  
Atomic Hydrogen ◽  
Hydrogen Generation ◽  
Hydrogen Plasma
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