scholarly journals Genealogical structure of the Belarusian black-and-white breed based on the microsatellite analysis of the lines bred at gene pool enterprises

2021 ◽  
Vol 65 (3) ◽  
pp. 369-379
Author(s):  
I. F. Gridyushko ◽  
A. A. Balnikov ◽  
I. P. Sheyko ◽  
O. Y. Vasilyuk ◽  
E. S. Gridyushko
Keyword(s):  
Gene Pool ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Microsatellite Analysis ◽  
Genomic Research ◽  
Dna Testing ◽  
Black And White ◽  
White Breed ◽  
Dynamics Of Population ◽  
Breeding Schemes

The methods of molecular genetic analysis are widely used to assess the genetic structure and to study the dynamics of population-genetic processes in populations of domestic animals. I n particular, these are the highly polymorphic genetic markers – the microsatellites that are the most common molecular markers in genetic and genomic research. The aim of the research was to develop the genealogical structure of the Belarusian black-and-white breed based on the microsatellite analysis of the lines bred at a gene pool enterprise. The research was carried out with the animals bred at the JSC “SGC “Zarechye”. For the microsatellite analysis of boars of the Belarusian black-and-white breed, the DNA testing was carried out at 9 STR-loci (SО155, SО355, SО386, SО005, SW72, SW951, SО101, SW240, and SW857). In all studied lines, the largest number of effective alleles was at the SО005 locus – 4.00–5.14. The lines of animals with heterozygosity can be used in various breeding schemes, both in purebred breeding (in pedigree pig breeding) and in crossing (in industrial pig breeding). Polymorphism determines the degree of genetic variation in populations. The locus is classified as polymorphic when the frequency of the most common allele of this locus does not exceed 95% (р ≤ 95). The revealed polymorphism of loci in the lines Maket, Tik, and Veseliy indicates the presence of genetic variability in them, which allows using them in breeding and breed-forming processes. Based on the studies made, the genealogical structure of the Belarusian black-and-white breed, consisting of one subcluster and two branches, has been developed, which makes it possible to assess the state and degree of relation of structural units (lines), and reflects the prospect of their further use.

scholarly journals The pattern of genetic diversity of different breeds of pigs based on microsatellite analysis

2020 ◽  
Vol 24 (7) ◽  
pp. 747-754
Author(s):  
V. R. Kharzinova ◽  
N. A. Zinovieva
Keyword(s):  
Genetic Diversity ◽  
Genetic Relationships ◽  
Molecular Genetic ◽  
Genetic Material ◽  
Animal Husbandry ◽  
Molecular Genetic Analysis ◽  
Fixation Index ◽  
Large White ◽  
Black And White ◽  
The Republic

One of the main tasks of genetics and animal breeding is the assessment of genetic diversity and the study of genetic relationships between different breeds and populations using molecular genetic analysis methods. We analysed the polymorphism of microsatellites and the information on the state of genetic diversity and the population structure of local breeds in Russia: the Kemerovo, the Berkshire, the Liven, the Mangalitsa, and the Civilian; in the Republic of Belarus: the Large White and the Black-and-White; and in Ukraine: the White Steppe, as well as commercial breeds of imported origin of domestic reproduction: the Large White, the Landrace, and the Duroc. The materials used for this study were the tissue and DNA samples extracted from 1,194 pigs and DNA of the UNU “Genetic material bank of domestic and wild animal species and birds” of the L.K. Ernst Federal Research Center for Animal Husbandry. Polymorphisms of 10 microsatellites (S0155, S0355, S0386, SW24, SO005, SW72, SW951, S0101, SW240, and SW857) were determined according to the previously developed technique using DNA analyser ABI3130xl. To estimate the allele pool of each population, the average number of alleles (NA), the effective number of alleles (NE ) based on the locus, the rarified allelic richness (AR), the observed (HO ) and expected (HE ) heterozygosity, and the fixation index (FIS) were calculated. The degree of genetic differentiation of the breeds was assessed based on the pairwise values of FST and D. The analysis of the allelic and genetic diversity parameters of the local breeds showed that the maximum and minimum levels of polymorphism were observed in pigs of the Ukrainian White Steppe breed (NA = 6.500, NE = 3.709, and AR = 6.020) and in pigs of the Duroc breed (NA = 4.875, NE = 2.119, and AR = 3.821), respectively. The highest level of genetic diversity was found in the Large White breed of the Republic of Belarus (HO = 0.707 and NE = 0.702). The minimum level of genetic diversity was found in pigs of the imported breeds – the Landrace (HO = 0.459, HE = 0.400) and the Duroc (HO = 0.480, HE = 0.469) – indicating a high selection pressure in these breeds. Based on the results of phylogenetic analysis, the genetic origin of Large White pigs, the breeds, from which the Berkshire pigs originated, and the genetic detachment of the Landrace from the Mangalitsa breeds were revealed. The cluster analysis showed a genetic consolidation of the Black-and-White, the Berkshire, and the Mangalitsa pigs. Additionally, the imported breeds with clustering depending on the origin were characterised by a genetic structure different from that of the other breeds. The information obtained from these studies can serve as a guide for the management and breeding strategies of the pig breeds studied, to allow their better use and conservation.

Dynamics of the live weight of replacement heifers depending on their origin from the sire

2021 ◽  
pp. 24-30
Author(s):  
O. V. Gorelik ◽  
N. A. Fedoseeva ◽  
A. S. Gorelik ◽  
E. V. Koksharov
Keyword(s):  
Dairy Cattle ◽  
Gene Pool ◽  
Live Weight ◽  
Age Dynamics ◽  
Breeding Stock ◽  
Black And White ◽  
Holstein Breed ◽  
White Breed ◽  
Intensive Rearing ◽  
Selection Of

In the last few decades the improvement of domestic dairy cattle was due to the widespread use of the gene pool of the best world breed such as Holstein. As a result of crossbreeding the breeding stock of the domestic Black-and-White breed with the sires of Holstein breed in different regions of the country a population of crossbreds has been created. The purpose of the work was to study the age dynamics of the live weight of replacement heifers depending on the paternal origin and the correlation between the indicators for the growth periods. The daughters of different sires have a difference in their live weight at the first insemination. The live weight of more than 400 kg was obtained from replacement heifers obtained from the sires Saber, Decaro and Baquero. The daughters of the sires Gatedenser, Bentley and Alta Ciara in live weight were inferior to their herdmates by 2,7–9,3 kg or by 0,7–2,8 % (P ≥ 0,05). The bulk of the replacement heifers are inseminated at the age of 13 months and only the daughters of the sire Alta Ciara are inseminated at the age of 14,1 months with a live weight of 396,7 kg. Despite the almost identical live weight of heifers at birth they differed from each other in the process of rearing. It has been established that the live weight at birth is not an indicator for assessing the further growth of replacement young animals. At 6 months of age the correlation of live weight at the age of 6 months and at the first insemination was positive and ranges from 0,01 (daughters of the sire Baquero) to 0,55 (daughters of the sire Decaro). Selection of heifers at the age of 6 months by their live weight allows us to choose the best and provide the opportunity to inseminate them at the age of 12–13 months with the organization of intensive rearing.

scholarly journals MILK OF BLACK-WHITE COWS WITH DIFFERENT GENES OF CAPPA CASEIN AND DIACYLGLYCEROL OF O-ACYLTRANSFERASE FOR CHEESEMAKING

2020 ◽  
Vol 5 (2) ◽  
pp. 59-63
Author(s):  
Radik Shaydullin ◽  
Gazimzyan Sharafutdinov ◽  
Anastasiy Moskvicheva
Keyword(s):  
Dna Testing ◽  
Coagulation Time ◽  
Allelic Variants ◽  
Cheese Making ◽  
Black And White ◽  
Dgat1 Gene ◽  
White Breed ◽  
The Republic ◽  
Milk Coagulation ◽  
Time Type

The purpose of the research is improving the quality and technological properties of milk from black-and-white cows. The research was conducted on first-calf cows of a black-and-white breed in LLC «Dusym» of the Atninsky district of the Republic of Tatarstan. Three groups of experimental cows were formed depending on the genotype of CSN3 and DGAT1, which have equal lactation month. Cheese making milk obtained from cows with different allelic variants of the kappa-casein and diacylglycerol O-acyltransferase genes was studied. DNA testing was performed on the CSN3 and DGAT1 genes of experimental cows and allelic variants of these genes were determined by pol-ymerase chain reaction followed by analysis of restriction-site polymorphism. It was found that milk of cows with the genotype CSN3 AB and CSN3 BB, when exposed to rennet, showed a better yield of a dense casein clot (80 and 100%) and separation of serum (P <0.05), with a shorter coagulation time (20.3 and 16.5 min; P <0.05-0.01). Ac-cording to the DGAT1 gene, first-calf milk with the allelic variant DGAT1 AK and DGAT1 KK had the advantage, respectively, 86.7% and 85.7%, 24.0 and 25.7 minutes. The worst milk coagulation time is typical for CSN3 AA group – 29.5 minutes and DGAT1 AA one – 30.4 min. In terms of milk coagulation time, type II (15-40 min.) groups have the genotype CSN3 AB and CSN3 BB with the largest share of cows in the herd – 80.0 and 71.4% and with the genotype DGAT1 AK and DGAT1 KK – 86.6 and 85.7%, respectively. Thus, milk obtained from cows with the kappa-casein gene allele B and the diacylglycerol O-acyltransferase gene allele K gene in the genotype has the best cheese-making properties.

Molecular Characterization and Genetic Diversity Study in F3 Population of Rice

2013 ◽  
Vol 20 (1-2) ◽  
pp. 1-8
Author(s):  
MM Rahman ◽  
L Rahman ◽  
SN Begum ◽  
F Nur
Keyword(s):  
Genetic Distance ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Polymorphic Loci ◽  
Rice Genotypes ◽  
High Level ◽  
Genetic Diversity Study ◽  
Diversity Study

Random Amplified Polymorphic DNA (RAPD) assay was initiated for molecular genetic analysis among 13 F3 rice lines and their parents. Four out of 15 decamer random primers were used to amplify genomic DNA and the primers yielded a total of 41 RAPD markers of which 37 were considered as polymorphic with a mean of 9.25 bands per primer. The percentage of polymorphic loci was 90.24. The highest percentage of polymorphic loci (14.63) and gene diversity (0.0714) was observed in 05-6 F3 line and the lowest polymorphic loci (0.00) and gene diversity (0.00) was found in 05-12 and 05-15 F3 lines. So, relatively high level of genetic variation was found in 05-6 F3 line and it was genetically more diverse compared to others. The average co-efficient of gene differentiation (GST) and gene flow (Nm) values across all the loci were 0.8689 and 0.0755, respectively. The UPGMA dendrogram based on the Nei’s genetic distance differentiated the rice genotypes into two main clusters: PNR-519, 05-19, 05-14, 05-12 and 05-17 grouped in cluster 1. On the other hand, Baradhan, 05-9, 05-13, 05-11, 05-5, 05-6, 05-1, 05-4, 05-15 and 05-25 were grouped in cluster 2. The highest genetic distance (0.586) was found between 05-4 and 05-17 F3 lines and they remain in different cluster.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16839 Progress. Agric. 20(1 & 2): 1 – 8, 2009

Diagnosis and Management of Cardiomyopathies – A Focus on Genetics, Cardiac Magnetic Resonance and Clinical Features

2011 ◽  
Vol 7 (3) ◽  
pp. 225
Author(s):  
Gianfranco Sinagra ◽  
Michele Moretti ◽  
Giancarlo Vitrella ◽  
Marco Merlo ◽  
Rossana Bussani ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Cardiac Magnetic Resonance ◽  
Magnetic Resonance ◽  
Imaging Techniques ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Routine Clinical Practice ◽  
Clinical Approach ◽  
Diagnosis And Management ◽  
Made In

In recent years, outstanding progress has been made in the diagnosis and treatment of cardiomyopathies. Genetics is emerging as a primary point in the diagnosis and management of these diseases. However, molecular genetic analyses are not yet included in routine clinical practice, mainly because of their elevated costs and execution time. A patient-based and patient-oriented clinical approach, coupled with new imaging techniques such as cardiac magnetic resonance, can be of great help in selecting patients for molecular genetic analysis and is crucial for a better characterisation of these diseases. This article will specifically address clinical, magnetic resonance and genetic aspects of the diagnosis and management of cardiomyopathies.

The improvement of the type Karatomar of Black-and-White cattle breed under the conditions of Northern Kazakhstan

2020 ◽  
pp. 23-31
Author(s):  
L. Ovchinnikova ◽  
E. Babich
Keyword(s):  
Research Work ◽  
Cattle Breed ◽  
Reproductive Traits ◽  
Farm Animals ◽  
Development Indicators ◽  
Black And White ◽  
Breed Type ◽  
Northern Kazakhstan ◽  
White Breed ◽  
White Cattle

In the Republic of Kazakhstan over the past decade positive changes have been observed in increasing the number of farm animals, increasing their productivity, and increasing the gross production of milk, meat and other products. At the same time the development of dairy cattle breeding is mainly due to the breeding of Black-and-White, Red Steppe, Simmental breeds and their intra-breed types as well as imported from abroad Holstein breed. The purpose of the research was to analyze ways to improve the intra-breed type Karatomar of Black-and-White breed in Northern Kazakhstan. Research work has been carried out on breeding animals such as Karatomar type cattle of Black-and-White breed in LLP “Experimental farm Zarechnoe” in the Kostanay district in the Kostanay region. The dynamics of growth and development indicators, reproductive traits of heifers of a new intra-breed type Karatomar of Black-and-White cattle have been studied. A comprehensive assessment of their economically useful traits in the first lactation, determined by the resistance of animals to climatic and forage conditions, the efficiency of breeding of animals of different genotypes has been conducted. It has been found that the replacement heifers – daughters of American breeding sires inherited precocity and showed higher growth energy after 6 months of age and continued to grow rapidly until 18 months. The advantage in metabolism was in the daughters of Holstein sires of the American selection Orbit 4078, and the increased immune status of the organism in the daughters of the sire of the domestic selection Yamal 975 as the most adapted to the conditions of Northern Kazakhstan. The best ability to increasing the milk yield, stable lactation activity, high milk productivity, gross content of dry matter and nonfat milk solids have been characterized by the daughters of sires of the American selection Orbit 4078 and Lowrider 4129. Analysis of economic indicators of milk production has been shown that from cows – daughters of sires Orbit 4078 and Lowrider 4129 for 305 days of lactation received 15,8 and 15,2 % more milk of base fat, the expenditures of EFU per 1 kg of production was lower by 10,2 and 5,9 %. A higher level of profitability has been observed in the group of daughters of the sires Orbit 4078 – 35,5 % and Lowrider 4129 – 33,1 %, which was 8,5 and 10,8 % higher than in the group of daughters of the sire Yamal 975.

The influence of probiotic drug Biolax-U on morphological and biochemical parameters of blood in cows

2020 ◽  
pp. 70-76
Author(s):  
A. Farkhutdinova
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Experimental Studies ◽  
Positive Influence ◽  
Biologically Active ◽  
Feed Consumption ◽  
Control Group ◽  
Animal Blood ◽  
Black And White ◽  
White Breed

The expediency of using the probiotic drug Biolax-U in the rations of lactating and dry cows of Black-and-White breed has been studied and scientifically proved. The influence of this drug on the morphological and biochemical parameters of animal blood has been determined. The composition of the probiotic drug Biolax-U consists of eight strains of a balanced complex of living lactic acid bacteria, yeast and actinomycetes, as well as biologically active substances. For experimental studies conducted on 2 groups of cows have been selected by the method of pairs-analogs, the influence of feeding of feed have been prepared with the use of the microbiological drug with probiotic action Biolax-U on feed consumption, morphological and biochemical changes in blood has been studied. Analysis of the rations of cows indicates that the content of calcium, phosphorus, magnesium, sulfur and trace elements such as manganese, copper, zinc and cobalt in the feed has been reduced. It has been found by studies that the use of probiotic drug Biolax-U had the positive influence on the feed intake, digestibility and better assimilation of feed nutrients and contributed to the improvement of blood morphological and biochemical parameters. For example, in animals of the experimental group hemoglobin has increased by 3,6 % (Р < 0,05), red blood cells by 10,1 (P ≤ 0,05), the content of leukocytes by 4,43, carotene by 9,6, glucose by 3,4; reserve alkalinity by 15,6, calcium by 19,4 (P ≤ 0,01), and phosphorus by 18,4 % compared with the control group of animals. During the lactation significantly improved hematological parameters including alkalinity reserve by 5,11 %, calcium level by 11,1, phosphorus by 8,6, hemoglobin by 14,2 %, the content of erythrocytes by 12,3, leukocyte by 3,86, and carotene 11,1 % against the parameters of animals of control group. The improvement of morphological and biochemical parameters of blood of cows indicates about stimulating effect on hematopoiesis and the general clinical condition of animals that contributes to the improvement of metabolic processes when using feed prepared with the use of microbiological drug with probiotic action Biolax-U.

Extruded lupine as a part of energy-protein concentrate in the ration of lactating cows

2020 ◽  
pp. 65-73
Author(s):  
Z. Fedorova
Keyword(s):  
Farm Animals ◽  
Feed Consumption ◽  
Control Group ◽  
Protein Concentrate ◽  
Cattle Farm ◽  
Black And White ◽  
Lactating Cows ◽  
White Breed ◽  
Kaliningrad Region ◽  
Experimental Group

In order to fi ll the existing protein defi ciency in the rations of farm animals in particular dairy cows it is necessary to input feed lupins as widely as possible in crop rotations. Lupin is a very versatile forage crop it can be used in feed as herbage, in the form of haylage, silage, in compound feed as a protein additive, as well as a green manure crop to increase soil fertility. Questions of protein nutrition should be to be quickly resolved, both in the production of protein and in its standardized rational use. Unbalanced protein rations increase feed consumption per unit of production, thereby increasing its prime cost. In the world soya is generally recognized as a protein feed, but in the Kaliningrad region as in most of regions in our country, it does not maturate being a heat-loving crop. Under these environments the inclusion of extruded lupine grain in the composition of energy-protein concentrates for the production of compound feed has become an alternative to replacing expensive soya. The researches have been carried out it the cattle farm of LLC “Temp” in the Guryevsky area in the Kaliningrad region (March-May 2019) on feeding lactating cows energy-protein concentrate from extruded grain of narrow-leaved lupine, fl ax, triticale, subjected to heat treatment on a press extruder. For this purpose two groups of lactating cows of Black-and-White breed (control and experimental) have been formed using the method of pairs of analogs per 11 heads in each. Cows of the control group have received the main ration of the farm, and cows of the experimental group in addition to the main ration have received compound feed with extruded energy-protein concentrate based on lupine. As a result of research, the positive infl uence of energy-protein concentrate based on lupine in compound feed on the dairy productivity of cows has been established. Recipes of concentrate and compound feed with concentrate for lactating cows, the volume of inclusion of components in the concentrate and in the compound feed have been calculated. During the experiment, the milk yield in the experimental group of cows has increased by 62,4 kg or 8,0 %, than in the control group. From the cows of the experimental group the conditional profi t has been received in the amount of 1684,8 rubles per head.

Sign in / Sign up

Export Citation Format

Share Document