The Effect of Platycodon grandiflorum Root Ethanol Extract on Blood Glucose, Lipid, Activities of Carbohydrate Metabolism Related Enzyme in Streptozotocin-Induced Diabetic Rats

2016 ◽  
Vol 33 (4) ◽  
pp. 686-692 ◽  
Author(s):  
Ok-Kyung Kim
Keyword(s):  
Blood Glucose ◽  
Carbohydrate Metabolism ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
Platycodon Grandiflorum ◽  
Related Enzyme

scholarly journals ANTIDIABETIC AND ANTIHYPERLIPIDAEMIC ACTIVITY OF NELUMBO NUCIFERA GAERTN ETHANOL SEED EXTRACT IN STREPTOZOTOCIN INDUCED DIABETIC RATS

2017 ◽  
Vol 9 (10) ◽  
pp. 197
Author(s):  
Alok Bhardwaj ◽  
Ketan. P. Modi
Keyword(s):  
Antioxidant Activity ◽  
Blood Glucose ◽  
Lipid Peroxides ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
Seed Extract ◽  
Serum Enzymes ◽  
Nelumbo Nucifera ◽  
Albino Rats ◽  
Intraperitoneal Dose

Objective: The main objective of the study was to investigate the effect of ethanol seed extracts of Nelumbo nucifera Gaertn (Nymphaeaceae) as antihyperglycemic, anti-hyperlipidemic and antioxidant activity in streptozotocin-induced diabetic rats.Methods: Diabetes was induced in wistar albino rats by administration of streptozotocin (single intraperitoneal dose of 60 mg/kg B. W). The ethanol extract of N. nucifera seed at a dose of 200 and 400 mg/kg body weight was administrated at a single dose per day to STZ induced diabetic rats for a period of 42 d. The different pharmacological parameters were evaluated. The effect of ethanol seed extract of N. nucifera on insulin, blood glucose, urea, creatinine, HbA1C, serum protein, albumin, globulin, serum enzymes, serum lipid profiles, lipid peroxides (LPO) and other antioxidant enzymes like catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) and reduced glutathione (GSH) were measured in the diabetic rats.Results: In the acute toxicity study ethanol seed extract of Nelumbo nucifera were non-toxic at 2000 mg/kg in rats. The ethanol extract of Nelumbo nucifera seed showed significant reduction in blood glucose (p<0.05), serum enzymes (SGPT, SGOT, ALP) (p<0.05), lipid parameters (TC, TG, VLDL, LDL) (p<0.05) except HDL and significantly increased insulin (p<0.05), HDL (p<0.05), GPx, GSH, SOD and CAT (p<0.05) at the dose of 400 mg/kg when compared with the diabetic-induced control.Conclusion: The present study suggested that the NNSE has significant (p<0.05) antihyperglycemic, antihyperlipidemic and antioxidant activity in STZ induced diabetic rats. These results clearly indicate that Nelumbo nucifera is effective against free radical-mediated diseases, thus replacing the synthetic ones.

scholarly journals Evaluation the Effect of Vernonia amygdalina. Del Leaves Ethanol Extract on Blood Sugar and its Synergism with Glibenclamide in Rats

2020 ◽  
Vol 13 (1) ◽  
pp. 26-34
Author(s):  
Igwe K ◽  
Ikpeazu O ◽  
Otuokere I
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Vernonia Amygdalina ◽  
Treatment Groups ◽  
Glucose Levels

Antidiabetic activity of Vernonia amygdalina and its possible synergism with glibenclamide was checked. Forty eight rats were used for the research, for hypoglycermic study of V. amygdalina alone, they were grouped into five of six rats each. Group 1 was the negative control and was administered distilled water orally. Groups 2, 3, and 4 were the treatment groups which received 100, 200 and 300 mg/kg body weight of the V. amygdalina extract respectively orally by intubation. Group 5 was the positive control group which received a known antidiabetic drug, glibenclamide. Diabetes was induced with alloxan. For the synergism study, another 18 rats grouped into 3 of six rats each was used. Both groups of glibenclamide only and glibenclamide plus V. amygdalina extract were dosed for 14 days orally by intubation, thereafter were sacrificed and blood collected from heart for analysis. There were 5 replicates grouped by weight throughout the study and both single and synergistic studies had the same controls. Effect of V. amygdalina extract was checked on blood glucose and its possible synergism with glibenclamide. All results in treatment groups were compared with the normal control at statistical confidence of p<0.05. Result shows that V. amygdalina extract reduced blood glucose level in the test groups as dose of extract increased. Combination of V. amygdalina with glibenclamide demonstrated further deduction in blood glucose levels in the treatment rats groups. Therefore addition of V. amygdalina into glibenclamide increased efficacy in the diabetic rats. The interaction between V. amygdalina and glibenclamide in this work was additive and therefore synergistic.

scholarly journals THE EFFECTIVENESS OF ETHANOL EXTRACT OF BINAHONG LEAVES ON DIABETIC WOUND HEALING

2018 ◽  
Vol 11 (4) ◽  
Author(s):  
Devita Anggraeni ◽  
Claude Mona Airin ◽  
Slamet Raharjo
Keyword(s):  
Blood Glucose ◽  
Leukocyte Count ◽  
Ethanol Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Dorsal Skin ◽  
Group I ◽  
Diabetic Control Group ◽  
Chemical Profiles

This research aimed to study the effectiveness of ethanol extract of binahong leaves on blood glucose, insulin, blood chemical profiles (serum glutamic pyruvate transaminase=SGPT, serum glutamic oxaloacetic transaminase=SGOT, ureum, and creatinine), and skin histopathology in diabetic rat. A total of 20 male Wistar rats aged 3 months (± 250 gram) were divided into five groups, with four rats in each group. Group I (non-diabetic control) was injected with 0.1 M sodium citrate buffer, while group II (diabetic control), III, IV, and V were injected with single dose of Streptozotocin (STZ) at dose 40 mg/kg intraperitoneally (IP). One week after the injection, the dorsal skin of the rats were excised. Group I and II were given cream topically and 1% NaCMC orally, group III was given 50% ethanol extract of binahong leaves (EEB) topically and 1% NaCMC orally, group IV was given cream topically and EEB 300 mg/kg orally, and group V was given 50% EEB topically and EEB 300 mg/kg orally. These treatments were continued for 14 days. Blood samples were obtained at the end of study to examine blood glucose, insulin, and blood chemical profiles (SGOT, SGPT, ureum, and creatinine). Examination of skin histopathology and leukocyte count were also done. The result showed that blood glucose, insulin, SGOT, SGPT, and ureum level of diabetic rats given topical or oral EEB did not significantly different from diabetic control group, even though blood glucose, insulin, SGOT, SGPT, and ureum level of diabetic rats given topical and oral EEB were found lower compared to diabetic control group. Administration of EEB 300 mg/kg orally in diabetic rats could lower creatinine level significantly (P<0.05). Histopathological examination of dorsal skin of diabetic rats which were given EEB topically showed the decrease of fibroblast proliferation, leukocyte infiltration, and hemorrhage in dermis area. Leukocyte count on skin tissue was significantly lower (P<0.05) in diabetic rats given EEB. In conclusion, topical or oral administration of EEB can help healing process in diabetic wound.

scholarly journals ANTIDIABETIC AND ANTIHYPERLIPIDAEMIC EFFECTS OF THE ETHANOL EXTRACT OF THE LEAVES AND STEM OF CISSUS GRACILLIS (GULL ET PERR) (VITACEAE)

2021 ◽  
pp. 54-56
Author(s):  
OLUSAYO A SHORINWA ◽  
GORDON EI EMENU
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Phytochemical Screening ◽  
Glucose Levels ◽  
Diabetic Control Group ◽  
Alloxan Monohydrate

Objectives: This study investigated the antidiabetic and antihyperlipidemic potential of the ethanol extract of the leaves and stem of Cissus gracillis on alloxan monohydrate-induced diabetic albino rats. Methods: Preliminary phytochemical screening and acute toxicity were carried out. Animals were assigned into seven groups of five rats each. Groups A and B were administered 10 mg/kg each of glibenclamide and atorvastatin respectively, C, D, and E were given 125, 250 and 500 mg/kg of ethanol extract of C. gracillis, respectively, daily for 21 days through oral gavage, group F was diabetic but untreated (diabetic control group), while group G was non-diabetic and untreated which served as the control group. Results: Phytochemical screening revealed the presence of steroids/triterpenoids and carbohydrates. LD50 was above 5000 mg/kg. The extract at 500 mg/kg showed a statistically significant (p<0.05) decrease in blood glucose level when compared with the glibenclamide group on day 21. However, gradual non- significant reduction in blood glucose levels were observed in the extract treated groups on the 7th, 14th, and 21st days of treatment. The administration of ethanol extract of C. gracillis to alloxan-induced diabetic rats produced a decrease in total cholesterol, triglycerides, and low-density lipoproteins comparable to glibenclamide and atorvastatin. Conclusion: The ethanol extract of the leaves and stem of C. gracillis possess a mildly significant antidiabetic and antihyperlipidemic activity.

scholarly journals Modulations of Some Carbohydrate Metabolic Enzymes by Aqueous and Ethanol Buchholzia coriacea Seed Extract in Alloxan Induced Diabetic Rats

2018 ◽  
pp. 1-9
Author(s):  
K. O. Gbagbeke ◽  
A. O. Naiho ◽  
P. R. C. Esegbue ◽  
M. O. Odigie ◽  
M. A. Omoirri
Keyword(s):  
Blood Glucose ◽  
Aqueous Extract ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Metabolic Enzymes ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Total Carbohydrate ◽  
Adult Rats ◽  
Ethanol Extracts

Characterised by abnormal increase in blood glucose level, Diabetes is a metabolic disorder that is associated with complications in carbohydrate, protein and fat metabolism. In recent times, medicinal herbs have been implicated in traditional medical practice for the treatment of this ailment. Studies have shown that Buchholzia coriacea seed possesses some anti-hyperglycemic properties that may be useful in the management of diabetes. To this point, present study investigated the effect(s) of oral administration of aqueous and ethanol extracts of Buchholzia coriacea on some carbohydrate metabolism parameters in normal and alloxan-induced diabetic rats. Forty (40) adult rats of both sexes were randomly assigned into two groups (normoglycemic and hyperglycemic). While group 1 (normoglycemic) had normal control, metformin, aqueous extract (250mg/kg) and ethanol extract (250 mg/kg) treated sub-groups respectively, Group 2 (hyperglycemic) contained the diabetic control, metformin, aqueous extract (250 mg/kg), and ethanol extracted (250mg/kg) treated sub-groups dosed daily by oral gavage for 14 days. At the end of the treatment, rats were euthanized via cervical dislocation; blood samples were collected by cardiac puncture for statistical analysis. One-way analysis of variance (ANOVA) revealed that dosing with extracts had insignificant effect(s) on body weight of rats. Fasting Blood Glucose (FBG) levels were elevated before and after extracts administration. Metformin, aqueous and ethanol extracts significantly reduced (p<0.05) FBG levels. Also, compared with control, total carbohydrate, liver glucose, glycosylated haemoglobin, Lactate Dehydrogenase, Isocitrate dehydrogenase, MDH, SDH, 6-phosphogluconate dehydrogenase, G6PD and CcO activities were significantly reduced (p<0.05) in diabetic treated rats. Buccholzia Coriacea was therefore seen to pose hypoglycemic and glycolytic effects, regulating activities of carbohydrate metabolic enzymes. Apparently, there is a scientific merit in the use of the extract in the management of diabetes.

scholarly journals Antidiabetic activity of musa acuminata colla fruit peel (MACFP) ethanol extract in glucose-induced diabetic rats

2018 ◽  
Vol 197 ◽  
pp. 07007
Author(s):  
Erza Genatrika ◽  
Visda Nila Happy Laksari ◽  
Tjiptasurasa Tjiptasurasa
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Ethanol Extract ◽  
Positive Control ◽  
Diabetic Rats ◽  
Musa Acuminata ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Fruit Peel

Diabetes mellitus is a metabolic disorder disease characterized by increased blood glucose levels due to impaired insulin secretion or increased insulin resistance. The study was performed to investigate the blood glucose lowering effect of Musa acuminata Colla fruit peel (MACFP) ethanol extract in experimentally induced diabetic rats. 25 rats were divided into five groups, i.e negative control group (0.5% NaCMC), positive control group (glibenclamide), and treatment group of MACFP ethanol extract with dose 250, 375, and 500 mg/kg body weight (mg/kg BW). The blood sample was taken from the lateral vein of the tail, and then blood glucose level was observed on 0, 30, 60, 90, and 120 minutes. The results provide information that MACFP ethanol extract with dose 250, 375, and 500 mg/kg BW have the same effect with a positive control (glibenclamide) in lowering blood glucose level on diabetic rats (p>0,05). MACFP ethanol extract with dose 500 mg/kg BW had the highest percentage of decrease in blood glucose level (42,62%), followed by doses 375 mg/kg BW (37,26%) and 250 mg/kg BW (24,12%).

scholarly journals ANTIDIABETIC ACTIVITY OF BACOLEPIS NERVOSA (WIGHT AND ARN.) DECNE. EX MOQ. EXTRACT ON ALLOXAN INDUCED DIABETIC RATS

2016 ◽  
Vol 8 (11) ◽  
pp. 320
Author(s):  
Arockia Jenecius Alphonse A. ◽  
Mohan V. R. ◽  
Doss A.
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
Serum Enzymes ◽  
Antidiabetic Activity ◽  
Albino Rats ◽  
Ethanol Extracts ◽  
Wistar Albino Rats ◽  
Alloxan Monohydrate ◽  
Lipid Parameters

Objective: The aim of this study was to investigate the effect of ethanol extracts of stem and leaf of Bacolepis nervosa as antihyperglycemic, anti-hyperlipidemic and antioxidant activity in alloxan-induced diabetic rats. Methods: Diabetes was induced in wistar albino rats by administration of alloxan monohydrate (150 mg/kg). The ethanol extract of B. nervosa leaf and stem at a dose of 150 and 300 mg/kg body weight was administrated at a single dose per day to diabetes-induced rats for a period of 14 d. The effect of ethanol extract of B. nervosa leaf and stem on blood glucose, insulin, urea, creatinine, HbA1C, serum protein, albumin, globulin, serum enzymes, serum lipid profiles, lipid peroxidase (LPO) and antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) were measured in the diabetic rats.Results: The ethanol extract of B. nervosa stem and leaf elicited significant reduction in blood glucose (p<0.001), serum enzymes (SGPT, SGOT, ALP) (p<0.01), lipid parameters (TC, TG, VLDL-LDL, PL) (p<0.01) except HDL-C and significantly increased insulin (p<0.01), HDL-C (p<0.05),, GPx, GSH, SOD and CAT (p<0.05) at the dose of 300 mg/kg when compared with the diabetic-induced control.Conclusion: From the above results, it is concluded that ethanol extracts of B. nervosa leaf and stem possesses significant antihyperglycemic, antihyperlipidemic and antioxidant effect in alloxan induced diabetic rats.

scholarly journals Ekstrak Etanol Kulit Buah Jengkol Menurunkan Kadar Glukosa dan Meningkatkan Hormon Insulin Tikus Diabetes Yang Diinduksi Streptozotocin

2019 ◽  
Vol 20 (1) ◽  
pp. 65
Author(s):  
Desak Made Malini ◽  
Madihah Madihah ◽  
Dita Aprila Khoirunnisa ◽  
Indriani Sasmita ◽  
Nining Ratningsih ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Blood Glucose ◽  
Reference Group ◽  
Ethanol Extract ◽  
Insulin Level ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Female Rats ◽  
Fruit Peel

Diabetes Mellitus (DM) is a disease with metabolism disorder signed by increasing of glucose blood (hyperglicemia), caused by disorder of insulin secretion and or increasing of insulin resistance. Therapy of jengkol’s fruit peel is an alternative treatment for DM. This study aims to determine the effect of ethanol extract of jengkol’s fruit peel to decrease glucose and increase insulin level of Wistar female rats which induced by streptozotocin (STZ). This research used the experimental method of Completely Randomized Design (RAL). Rat were first induced by single intravenous injection of STZ except Negative Control. Furthermore, rats were divided into six groups, namely Negative Control (NC), Positive Control (PC), Reference Group (RG) glibenclamide 10 mg/kg BW, and ethanol extract of jengkol’s fruit peel (EEOJFP) treatment at dose of 385 (P1), 770 (P2), and 1540 (P3) mg/kg BW with four replications each. Treatment was administered orally for 14 days. Data were analyzed by analysis of variance (ANAVA) with 95% confidence level and Duncan Multiple Range Test. The result showed that treatment of EEOJFP with dose of 385, 770 and 1540 mg/ kgBW decreased blood glucose 28.6%;65.4% and 59.1% successively, increased insulin as 54%; 230% and 206%. There was a protective effect of EEOJEF to level of blood glucose and insulin secretion of rat-STZ induced. The conclusion from this research is Ethanol extract of Jengkol’s fruit peel at dose 770 mg/kg BW is an effective dose in decreasing the level of glucose and increasing the level of insulin hormone in STZinduced diabetic rats. 

scholarly journals Hypoglycemic Activity of Ethanol Extract of Jasminum Grandiflorum Flowers in Vivo and Cytotoxicity of Its Chloroform Isolate in Vitro

2018 ◽  
Vol 3 (2) ◽  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Ethanol Extract ◽  
Diabetic Rats ◽  
Hypoglycemic Activity ◽  
Albino Rats ◽  
Chloroform Fraction ◽  
Dependent Manner ◽  
Flower Extract

Ethno pharmacological relevance: Traditionally different parts of Jasminum grandiflorum have been used to treat various ailments, including diabetes. However, antidiabetic potential of Jasminum grandiflorum on animal models of diabetes have not been evaluated. Aim of the study: The objective of this study was to determine antidiabetic potential of ethanol extract of leaves and flowers of Jasminum grandiflorum, and different fractions of the flower extract in rodent model of streptozotocin-induced diabetes. Materials and methods: Ethanol extract of both leaves and flowers of Jasminum grandiflorum were screened for the presence of various phytochemicals followed by acute and sub-acute toxicity in rats. Effect of Jasminum grandiflorum leaf and flower extracts on blood glucose level in normal albino rats, in glucose-overloaded healthy albino rats, and in streptozotocininduced diabetic rats was evaluated. Furthermore, based on preliminary results, fractionalization of the flower extract was carried out using petroleum ether, ethyl acetate, methanol, and chloroform. Different fractions were further tested for hypoglycemic activity in streptozotocin-induced diabetic rats. Results: Preliminary phytochemical evaluation suggested presence of various antidiabetic metabolites in both the extracts and were found to safe up to 5000 mg/kg dose. Flower extract (500 mg/kg, p.o.) demonstrated significant hypoglycemic effect than leaf extract (500 mg/kg, p.o.) in normal rats, glucose-overloaded rats, and streptozotocin-induced diabetic rats when compared to control. Long-term effect of different fractions of ethanol extract of Jasminum grandiflorum flowers in streptozotocin model suggested that all four fractions were able to reduce blood glucose level in a time-dependent manner at 200 mg/kg dose with chloroform fraction being highly significant (p<0.001) amongst all when compared to diabetic untreated rats. Chloroform isolate from Jasminum grandiflorum flowers demonstrated enhanced glucose uptake and dosedependent cytotoxicity in L6 cell line. Conclusion: The ethanol extract of Jasminum grandiflorum flowers as well as its various fractions have potential therapeutic value in treating diabetes, which may be due to the presence of various antidiabetic metabolites, by enhancing insulin secretion and antioxidant defense. These observations rationalize its use as ethnomedicine and hence can be considered in treating diabetes.

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