Recent perspectives of nanotechnology in burn wounds management: a review

2021 ◽  
Vol 30 (5) ◽  
pp. 350-370
Author(s):  
Ruan Na ◽  
Tian Wei
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Healing Process ◽  
Skin Barrier ◽  
Active Role ◽  
Qualitative Studies ◽  
Future Directions ◽  
Burn Wounds ◽  
The Us ◽  
Efficacious Drug

Objective: The burden of the management of problematic skin wounds characterised by a compromised skin barrier is growing rapidly. Almost six million patients are affected in the US alone, with an estimated market of $25 billion annually. There is an urgent requirement for efficient mechanism-based treatments and more efficacious drug delivery systems. Novel strategies are needed for faster healing by reducing infection, moisturising the wound, stimulating the healing mechanisms, speeding up wound closure and reducing scar formation. Methods: A systematic review of qualitative studies was conducted on the recent perspectives of nanotechnology in burn wounds management. Pubmed, Scopus, EMBASE, CINAHL and PsychINFO databases were all systematically searched. Authors independently rated the reporting of the qualitative studies included. A comprehensive literature search was conducted covering various resources up to 2018–2019. Traditional techniques aim to simply cover the wound without playing any active role in wound healing. However, nanotechnology-based solutions are being used to create multipurpose biomaterials, not only for regeneration and repair, but also for on-demand delivery of specific molecules. The chronic nature and associated complications of nonhealing wounds have led to the emergence of nanotechnology-based therapies that aim at facilitating the healing process and ultimately repairing the injured tissue. Conclusion: Nanotechnology-based therapy is in the forefront of next-generation therapy that is able to advance wound healing of hard-to-heal wounds. In this review, we will highlight the developed nanotechnology-based therapeutic agents and assess the viability and efficacy of each treatment. Herein we will explore the unmet needs and future directions of current technologies, while discussing promising strategies that can advance the wound-healing field

scholarly journals Enhancement of Fibroblast Proliferation, Vascularization and Collagen Synthesis in the Healing Process of Third-Degree Burn Wounds by Topical Arnebia euchroma, a Herbal Medicine

2013 ◽  
Vol 1 (2) ◽  
pp. 53-59
Author(s):  
Soheil Ashkani-Esfahani ◽  
Mohammad Hossein Imanieh ◽  
Aidin Meshksar ◽  
Mahsima Khoshneviszadeh ◽  
Ali Noorafshan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Healing Process ◽  
Volume Density ◽  
Fibroblast Proliferation ◽  
Burn Wound ◽  
Closure Rate ◽  
Burn Wounds ◽  
Arnebia Euchroma ◽  
Degree Burn

Introduction: The present study was conducted to evaluate the wound healing effect of Arnebia euchroma (AE) extract, which is traditionally used in some Indian, Chinese, and Iranian tribes, on histomorphometrical parameters involved in the healing process of third-degree burn wounds by using stereological analyses. Methods and Materials: In an experimental study, 48 female Sprague-Dawley rats, each with a standard third-degree burn wound on the posterior surface of the neck, were divided into four groups; AE10 and AE20 groups were treated with carboxymethylcellulose (CMC) gels which contained AE hydroalcoholic extract at the concentration of 10% and 20%, respectively; the untreated burned (UB) group, which received no treatment; and the gel-base treated group. Wound closure rate, fibroblast proliferation, volume density of collagen bundles, length density, and mean diameter of the vessels were measured. Results: Wound closure rate, fibroblast population, volume density of collagen bundles, and length density of vessels were significantly improved by AE10 and AE20 in comparison with the gel-base and UB groups (P value <0.05). Conclusion: Although previous investigations on the different aspects of the wound healing effects of AE and the results of this study exhibited the positive effects of topical Arnebia euchroma on third-degree burn wound, introducing AE as an alternative wound healing agent requires more investigations on its efficacy on human, safety, and possible adverse effects.[GMJ. 2012;1(2):53-59]

673 Effect of Subcutaneous Topical Ozone Therapy on Second Degree Burn Wounds in Rats: An Experimental Study

2021 ◽  
Vol 42 (Supplement_1) ◽  
pp. S193-S193
Author(s):  
Emre Karakaya ◽  
Aydincan Akdur ◽  
H Ebru ◽  
Ayvazoglu Soy ◽  
Alev Ok Atilgan ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Male Rats ◽  
Control Group ◽  
Ozone Therapy ◽  
Burn Wounds ◽  
Degree Burn ◽  
Significant Difference ◽  
Second Degree

Abstract Introduction Burn is one of the most severe traumas that causes coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burn may affect the patient’s survival and reduce the complications that may be seen. In the present study we aimed effects of subcutaneous ozone injection on second degree burn wound. Methods A total of 72 Sprague-Dawley male rats included in the study were divided randomly into three groups (control group (CG), silver sulfadiazine group (SG), ozone group (OG)) and each group was divided randomly two subgroups (as sacrificed on d7 and on d14).A deep second degree scald burns were created on the lower back. In CG subcutaneous 0.9% serum saline was injected daily into the burn area. In SG, burns were dressed with silver sulfadiazine daily and in OG subcutaneous ozone was injected daily into the burn area. Tissue hydroxyproline level measurement and histopathological evaluation were done. Results When the groups were compared in terms of weight change, no significant difference was found on the 7th and 14th days. In the evaluation made in terms of tissue hydroxyproline, tissue hydroxyproline level in OG was found to be significantly higher on both the 7th and 14th days (p &lt; 0.001). In histopathological evaluations, it was determined that wound healing in OG was significantly higher than in the other groups. Conclusions According to the results, subcutaneous ozone therapy is more effective than silver sulphadiazine in the healing process of second-degree burn wounds and it can be safely used in the treatment of burn wounds.

scholarly journals The Efficacy of Silver-Based Electrospun Antimicrobial Dressing in Accelerating the Regeneration of Partial Thickness Burn Wounds Using a Porcine Model

Polymers ◽  
2021 ◽  
Vol 13 (18) ◽  
pp. 3116
Author(s):  
Thien Do ◽  
Tien Nguyen ◽  
Minh Ho ◽  
Nghi Nguyen ◽  
Thai Do ◽  
...  
Keyword(s):  
Wound Healing ◽  
Burn Injury ◽  
Healing Process ◽  
Bactericidal Effect ◽  
Wound Healing Process ◽  
Burn Wounds ◽  
Partial Thickness Burn ◽  
Tissue Damages

(1) Background: Wounds with damages to the subcutaneous are difficult to regenerate because of the tissue damages and complications such as bacterial infection. (2) Methods: In this study, we created burn wounds on pigs and investigated the efficacy of three biomaterials: polycaprolactone-gelatin-silver membrane (PCLGelAg) and two commercial burn dressings, Aquacel® Ag and UrgoTulTM silver sulfadiazine. In vitro long-term antibacterial property and in vivo wound healing performance were investigated. Agar diffusion assays were employed to evaluate bacterial inhibition at different time intervals. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time-kill assays were used to compare antibacterial strength among samples. Second-degree burn wounds in the pig model were designed to evaluate the efficiency of all dressings in supporting the wound healing process. (3) Results: The results showed that PCLGelAg membrane was the most effective in killing both Gram-positive and Gram-negative bacteria bacteria with the lowest MBC value. All three dressings (PCLGelAg, Aquacel, and UrgoTul) exhibited bactericidal effect during the first 24 h, supported wound healing as well as prevented infection and inflammation. (4) Conclusions: The results suggest that the PCLGelAg membrane is a practical solution for the treatment of severe burn injury and other infection-related skin complications.

scholarly journals Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds

2018 ◽  
Author(s):  
Yasuhiko Kaita ◽  
Takehiko Tarui ◽  
Hideaki Yoshino ◽  
Takeaki Matsuda ◽  
Yoshihiro Yamaguchi ◽  
...  
Keyword(s):  
Wound Healing ◽  
Therapeutic Effect ◽  
Healing Process ◽  
Control Group ◽  
Type I ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing ◽  
Significant Difference ◽  
Regenerative Cells

AbstractThe purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs.These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings.

scholarly journals A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1153
Author(s):  
Verena Schneider ◽  
Daniel Kruse ◽  
Ives Bernardelli de Mattos ◽  
Saskia Zöphel ◽  
Kendra-Kathrin Tiltmann ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inflammatory Response ◽  
Healing Process ◽  
Three Dimensional ◽  
Source Model ◽  
Burn Wound ◽  
Thermal Burn ◽  
Burn Wounds

Burns affect millions every year and a model to mimic the pathophysiology of such injuries in detail is required to better understand regeneration. The current gold standard for studying burn wounds are animal models, which are under criticism due to ethical considerations and a limited predictiveness. Here, we present a three-dimensional burn model, based on an open-source model, to monitor wound healing on the epidermal level. Skin equivalents were burned, using a preheated metal cylinder. The healing process was monitored regarding histomorphology, metabolic changes, inflammatory response and reepithelialization for 14 days. During this time, the wound size decreased from 25% to 5% of the model area and the inflammatory response (IL-1β, IL-6 and IL-8) showed a comparable course to wounding and healing in vivo. Additionally, the topical application of 5% dexpanthenol enhanced tissue morphology and the number of proliferative keratinocytes in the newly formed epidermis, but did not influence the overall reepithelialization rate. In summary, the model showed a comparable healing process to in vivo, and thus, offers the opportunity to better understand the physiology of thermal burn wound healing on the keratinocyte level.

Alginate hydrogel enriched with Ambystoma mexicanum epidermal lipoxygenase-loaded pectin nanoparticles for enhanced wound healing

2019 ◽  
Vol 34 (8) ◽  
pp. 1171-1187
Author(s):  
Farnoush Oveissi ◽  
Naser Tavakoli ◽  
Mohsen Minaiyan ◽  
Mohammad Reza Mofid ◽  
Azade Taheri
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Healing Process ◽  
Ambystoma Mexicanum ◽  
Wound Healing Process ◽  
Clinical Use ◽  
Sustained Delivery ◽  
Alginate Hydrogel ◽  
Wound Site ◽  
Minimal Scarring

Epidermal lipoxygenase enzyme extracted from Ambystoma mexicanum (AmbLOXe) is known to accelerate the wound-healing process. AmbLOXe as a protein suffers from inactivation and losing its activity during formulation. Therefore, a delivery system that protects AmbLOXe from inactivation and preserves its activity is needed. We prepared AmbLOXe-loaded pectin nanoparticles (AmbLOXe Pec-NPs) and placed them into an alginate hydrogel. AmbLOXe Pec-NPs incorporation into the alginate hydrogel provides a means for controlled and sustained delivery of AmbLOXe to the wound site. Furthermore, the suitable swelling behavior and mechanical properties of AmbLOXe Pec-NPs alginate hydrogel make it feasible for clinical use. AmbLOXe Pec-NPs alginate hydrogel significantly enhanced the wound-healing process on the rat full-thickness excisional wounds, increased the rate of wound closure, enhanced the re-epithelialization and decreased the incidence of abnormal scarring. AmbLOXe Pec-NPs alginate hydrogel can be proposed as an effective wound hydrogel for improving wound healing with minimal scarring.

scholarly journals Dexpanthenol in Wound Healing after Medical and Cosmetic Interventions (Postprocedure Wound Healing)

2020 ◽  
Vol 13 (7) ◽  
pp. 138
Author(s):  
Julian Gorski ◽  
Ehrhardt Proksch ◽  
Jens Malte Baron ◽  
Daphne Schmid ◽  
Lei Zhang
Keyword(s):  
Wound Healing ◽  
Clinical Studies ◽  
New Technologies ◽  
Healing Process ◽  
Skin Barrier ◽  
Skin Barrier Function ◽  
Skin Damage ◽  
Published Evidence ◽  
Superficial Skin

With the availability of new technologies, the number of subjects undergoing medical and cosmetic interventions is increasing. Many procedures (e.g., ablative fractional laser treatment) resulting in superficial/minor wounds require appropriate aftercare to prevent complications in wound healing and poor cosmetic outcome. We review the published evidence of the usefulness of topical dexpanthenol in postprocedure wound healing and the associated mechanisms of action at the molecular level. A search in the PubMed and Embase databases was performed to query the terms dexpanthenol, panthenol, superficial wound, minor wound, wound healing, skin repair, and postprocedure. Search results were categorized as clinical trials and in vitro studies. In vitro and clinical studies provided evidence that topically applied dexpanthenol promotes superficial and postprocedure wound healing. Latest findings confirmed that dexpanthenol upregulates genes that are critical for the healing process. The gene expression data are of clinical relevance as evidenced by prospective clinical studies indicating that topical dexpanthenol accelerates wound healing with rapid re-epithelialization and restoration of skin barrier function following skin injury. It can therefore be inferred that topical dexpanthenol represents an appropriate and state-of-the-art treatment option for superficial postprocedure wounds, especially when applied early after the superficial skin damage.

scholarly journals EFFECT OF MANIHOT ESCULENTA AQUEOUS EXTRACT AND THERAPEUTIC ULTRASOUND IN ACCELERATING THE WOUND HEALING PROCESS IN VITRO

2019 ◽  
Vol 81 (4) ◽  
Author(s):  
Ulfah Anwar ◽  
Siti Pauliena Mohd Bohari
Keyword(s):  
Wound Healing ◽  
Ascorbic Acid ◽  
Aqueous Extract ◽  
Manihot Esculenta ◽  
Wound Closure ◽  
Healing Process ◽  
Therapeutic Ultrasound ◽  
Wound Healing Process ◽  
Time Interval

The aim of this research is to investigate the wound healing process in in vitro by combining the Manihot esculenta aqueous extract and therapeutic ultrasound. Firstly, the optimization seeding densities of HSF cell 1184 in six-well plate, and then followed by the scratch assay experiment. The scratched that made was treated with the remedial treatments (Manihot esculenta aqueous extract only; ascorbic acid+ therapeutic ultrasound; Manihot esculenta aqueous extract+ ascorbic acid; Manihot esculenta aqueous extract+ therapeutic ultrasound and also the combination of these three materials). The rate of wound closure was observed and analysed at a time interval of 0, 2, 4, 6, 8, 10 and 24 h by using image J software. Then, the cells viability were analysed using the MTT assay. The result showed that Manihot esculenta aqueous extract coupled with specific dose therapeutic ultrasound represents a significantly high rate of wound closure at 96.10 % with the cell numbers at 5.44×105 cells/mL when compared to the other combination therapy. The finding of this study revealed that Manihot esculenta aqueous extract 200 µg/mL and the therapeutic ultrasound specific dose (3 MHz, 300 mWatt/cm2, 50% in 5 min) have the potential in accelerating wound healing process of cells in in vitro.

scholarly journals Specific PKC βII inhibitor: one stone two birds in the treatment of diabetic foot ulcers

2018 ◽  
Vol 38 (5) ◽  
Author(s):  
Sushant Kumar Das ◽  
Yi Feng Yuan ◽  
Mao Quan Li
Keyword(s):  
Wound Healing ◽  
Protein Kinase ◽  
Peripheral Blood ◽  
Wound Closure ◽  
Type I Diabetes ◽  
Healing Process ◽  
Wound Healing Process ◽  
Peripheral Blood Flow ◽  
Type I ◽  
Diabetic Mice

To explore whether or not inhibition of protein kinase C βII (PKC βII) stimulates angiogenesis as well as prevents excessive NETosis in diabetics thus accelerating wound healing. Streptozotocin (STZ, 60 mg/kg/day for 5 days, i.p.) was injected to induce type I diabetes in male ICR mice. Mice were treated with ruboxistaurin (30 mg/kg/day, orally) for 14 consecutive days. Wound closure was evaluated by wound area and number of CD31-stained capillaries. Peripheral blood flow cytometry was done to evaluate number of circulating endothelial progenitor cells (EPCs). NETosis assay and wound tissue immunofluorescence imaging were done to evaluate the percentage of neutrophils undergoing NETosis. Furthermore, the expression of PKC βII, protein kinase B (Akt), endothelial nitric oxide synthase (eNOS), vascular endothelial growth factor (VEGF), and histone citrullation (H3Cit) were determined in the wound by Western blot analysis. Ruboxistaurin accelerated wound closure and stimulated angiogenesis in diabetic mice. The number of circulating EPCs was increased significantly in ruboxistaurin-treated diabetic mice. Moreover, ruboxistaurin treatment significantly decreases the percentages of H3Cit+ cells in both peripheral blood and wound areas. This prevented excess activated neutrophils forming an extracellular trap (NETs) formation. The expressions of phospho-Akt (p-Akt), phospho-eNOS (p-eNOS), and VEGF increased significantly in diabetic mice on ruboxistaurin treatment. The expressions of PKC βII and H3Cit+, on the other hand, decreased with ruboxistaurin treatment. The results of the present study suggest that ruboxistaurin by inhibiting PKC βII activation, reverses EPCs dysfunction as well as prevents exaggerated NETs formation in a diabetic mouse model; thereby accelerating the wound healing process.

Morphine Stimulates Wound Healing Via Mu Opioid Receptor and Promotes Wound Closure in Sickle Mice

Blood ◽  
2011 ◽  
Vol 118 (21) ◽  
pp. 2118-2118 ◽  
Author(s):  
Derek Vang ◽  
Marna Ericson ◽  
Michael A Ansonoff ◽  
John E Pintar ◽  
Robert P Hebbel ◽  
...  
Keyword(s):  
Wound Healing ◽  
Opioid Receptor ◽  
Vascular Function ◽  
Neurogenic Inflammation ◽  
Wound Closure ◽  
Healing Process ◽  
Mu Opioid Receptor ◽  
Dorsal Skin ◽  
Morphine Treatment ◽  
Mu Opioid

Abstract Abstract 2118 Painful non-healing leg ulcers in sickle cell disease (SCD) pose major treatment challenges for which there is no satisfactory therapy. We observed that the vascular and nerve architecture are abnormal, and mu opioid receptor (MOR) expression is decreased in the skin of BERK mice expressing sickle hemoglobin, as compared to HbA-BERK mice expressing normal human hemoglobin (Kohli et al., Blood, 2010). MOR mediates morphine-induced angiogenesis and analgesia, which are critical to the healing process. We hypothesized that MOR mediates the healing process and that MOR agonists such as morphine will promote healing by stimulating angiogenesis. Ischemic wounds (6 mm diameter) with impaired blood supply were created on the dorsal skin of sickle (BERK, and S+SAntilles) and control (HbA-BERK and C57BL6, respectively) mice; and mu-, delta-, and kappa-opioid receptor knockout (MOR-, DOR- and KOR-KO, respectively) mice and their 129S6 wild type controls. BERK and S+SAntilles did not survive ischemic wound surgery, but S+SAntilles survived non-ischemic, 4 mm punch biopsies on the leg. Wounds were treated topically twice a day with morphine, 3 mg/g Eucerin cream or with PBS-Eucerin cream. Periodic wound tracings were used to quantitate wound area with Adobe Photoshop and % area healed was calculated. Ischemic wounds (100% closure on d17 in control 129S6) took significantly longer to heal as compared to punch biopsies (100% closure on d8 in control C57BL6). Morphine significantly accelerated healing as compared to PBS-cream in control, S+SAntilles, DOR- and KOR-KO, but not in MOR-KO mice, suggesting that morphine-induced healing is MOR-dependent. Of note, PBS-treated ischemic wounds in MOR-KO healed significantly slower than 129S6 control wounds, indicating that MOR is involved in wound healing. Blood flow was significantly higher in the intact unwounded dorsal skin of MOR-KO as compared to 129S6 mice (p<0.001). Significant decrease occurred after wounding in both 129S6 and MOR-KO, which was restored to baseline in morphine-treated 129S6 but not in MOR-KO wounds on d20. Thus, morphine promotes vascular function, which appears to be mediated by MOR in the healing wounds. In PBS-cream treated punch biopsy wounds, 100% wound closure occurred on day 8 as compared to day 6 with morphine treatment in both C57BL6 control and S+SAntilles sickle mice. Laser scanning confocal microscopy (LSCM) of healed wound scars 30d after wounding revealed significantly increased blood vessels (∼2-fold) in morphine-treated as compared to PBS treated in both C57BL6 and S+SAntilles mice. A significant increase was also noted in PGP 9.5-immunoreactive (ir) nerve fibers and lymphatics in morphine-treated scars. Pro-inflammatory and vaso-active neuropeptides, substance P (SP) and calcitonin gene related peptide (CGRP) were increased ∼2-fold in PBS-treated wound scars 30d post-wounding as compared to baseline in S+SAntilles mice, suggestive of increased neurogenic inflammation. However both neuropeptides returned to baseline levels in morphine-treated wounds. Similarly, both SP- and CGRP-ir in 129S6 and MOR-KO were increased significantly in PBS-treated mice but not in morphine-treated as compared to baseline before wounding. It is noteworthy, that SP- and CGRP-ir were significantly higher in the unwounded skin of MOR-, DOR- and KOR-KO as compared to 129S6 control mice, suggesting that all three ORs are involved in the regulation of neuropeptides. Therefore, even though wound healing and blood flow were not influenced by morphine in MOR-KO, neuropeptides were responsive to morphine treatment in MOR-KO. Together, these data suggest that MOR downregulation may contribute to impaired wound healing and that other ORs are critical to regulate neuropeptides to control neurogenic inflammation in the skin, which may in turn influence the healing process. Thus, morphine orchestrates wound healing by stimulating vascular function via MOR and modulating neurogenic inflammation via ORs in the skin. Since increased inflammation and vasculopathy underlie wound pathobiology in SCD, morphine may have a therapeutic effect on healing leg ulcers. Disclosures: No relevant conflicts of interest to declare.

Sign in / Sign up

Export Citation Format

Share Document