Some Experiments Relating to Surface Damage and Internal Structure Induced by Fatigue : Studies on Fatigue Mechanism, 1st Report

Ultramicrotomy produces plastic deformation in the surfaces of microtomed TEM specimens which can not generally be observed unless special preparations are made. In this study, a typical biological composite of tissue (infundibular thoracic attachment) infiltrated in the normal manner with an embedding epoxy resin (Epon 812 in a 60/40 mixture) was microtomed with glass and diamond knives, both with 45 degree body angle. Sectioning was done in Portor Blum Mt-2 and Mt-1 microtomes. Sections were collected on formvar coated grids so that both the top side and the bottom side of the sections could be examined. Sections were then placed in a vacuum evaporator and self-shadowed with carbon. Some were chromium shadowed at a 30 degree angle. The sections were then examined in a Phillips 300 TEM at 60kv.Carbon coating (C) or carbon coating with chrom shadowing (C-Ch) makes in effect, single stage replicas of the surfaces of the sections and thus allows the damage in the surfaces to be observable in the TEM. Figure 1 (see key to figures) shows the bottom side of a diamond knife section, carbon self-shadowed and chrom shadowed perpendicular to the cutting direction. Very fine knife marks and surface damage can be observed.

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X-ray microtomography

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100107058 ◽

1988 ◽

Vol 46 ◽

pp. 998-999

Author(s):

H.W. Deckman ◽

B.F. Flannery ◽

J.H. Dunsmuir ◽

K.D' Amico

Keyword(s):

Computed Tomography ◽

Internal Structure ◽

Imaging Technique ◽

Three Dimensional ◽

Tissue Structure ◽

Individual Element ◽

X Ray ◽

Non Invasive ◽

Panoramic Imaging ◽

Three Dimensional Images

We have developed a new X-ray microscope which produces complete three dimensional images of samples. The microscope operates by performing X-ray tomography with unprecedented resolution. Tomography is a non-invasive imaging technique that creates maps of the internal structure of samples from measurement of the attenuation of penetrating radiation. As conventionally practiced in medical Computed Tomography (CT), radiologists produce maps of bone and tissue structure in several planar sections that reveal features with 1mm resolution and 1% contrast. Microtomography extends the capability of CT in several ways. First, the resolution which approaches one micron, is one thousand times higher than that of the medical CT. Second, our approach acquires and analyses the data in a panoramic imaging format that directly produces three-dimensional maps in a series of contiguous stacked planes. Typical maps available today consist of three hundred planar sections each containing 512x512 pixels. Finally, and perhaps of most import scientifically, microtomography using a synchrotron X-ray source, allows us to generate maps of individual element.

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The internal structure of medullated wool

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100111987 ◽

1984 ◽

Vol 42 ◽

pp. 388-389

Author(s):

Leo Barish

Keyword(s):

Light Microscopy ◽

Internal Structure ◽

Wool Fiber ◽

Guard Hair ◽

Bright Field ◽

Wool Fibers ◽

Thin Skin ◽

Air Pockets

Although most of the wool used today consists of fine, unmedullated down-type fibers, a great deal of coarse wool is used for carpets, tweeds, industrial fabrics, etc. Besides the obvious diameter difference, coarse wool fibers are often medullated.Medullation may be easily observed using bright field light microscopy. Fig. 1A shows a typical fine diameter nonmedullated wool fiber, Fig. IB illustrates a coarse fiber with a large medulla. The opacity of the medulla is due to the inability of the mounting media to penetrate to the center of the fiber leaving air pockets. Fig. 1C shows an even thicker fiber with a very large medulla and with very thin skin. This type of wool is called “Kemp”, is shed annually or more often, and corresponds to guard hair in fur-bearing animals.

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Quantitative surface damage studies by H.R.E.M.

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010015513x ◽

1989 ◽

Vol 47 ◽

pp. 632-633

Author(s):

S. R. Singh ◽

H. J. Fan ◽

L. D. Marks

Keyword(s):

Solar Wind ◽

Quantitative Analysis ◽

Surface Science ◽

Surface Damage ◽

Space Environment ◽

Oxygen Desorption ◽

The Past ◽

Diffusion Controlled ◽

Original Observation ◽

Substantial Interest

Since the original observation that the surfaces of materials undergo radiation damage in the electron microscope similar to that observed by more conventional surface science techniques there has been substantial interest in understanding these phenomena in more detail; for a review see. For instance, surface damage in a microscope mimics damage in the space environment due to the solar wind and electron beam lithographic operations.However, purely qualitative experiments that have been done in the past are inadequate. In addition, many experiments performed in conventional microscopes may be inaccurate. What is needed is careful quantitative analysis including comparisons of the behavior in UHV versus that in a conventional microscope. In this paper we will present results of quantitative analysis which clearly demonstrate that the phenomena of importance are diffusion controlled; more detailed presentations of the data have been published elsewhere.As an illustration of the results, Figure 1 shows a plot of the shrinkage of a single, roughly spherical particle of WO3 versus time (dose) driven by oxygen desorption from the surface.

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Study of Tip-Surface Interactions in Scanning Tunneling Microscopy (STM) by Reflection Electron Microscopy (REM)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100180355 ◽

1990 ◽

Vol 48 (1) ◽

pp. 320-321

Author(s):

W. Lo ◽

J.C.H. Spence ◽

M. Kuwabara

Keyword(s):

High Resolution ◽

Elastic Strain ◽

Tunneling Current ◽

Surface Damage ◽

Surface Interactions ◽

Graphite Surface ◽

Scanning Tunneling ◽

Large Area ◽

Tunneling Microscopy ◽

High Resolution Tem

Work on the integration of STM with REM has demonstrated the usefulness of this combination. The STM has been designed to replace the side entry holder of a commercial Philips 400T TEM. It allows simultaneous REM imaging of the tip/sample region of the STM (see fig. 1). The REM technique offers nigh sensitivity to strain (<10−4) through diffraction contrast and high resolution (<lnm) along the unforeshortened direction. It is an ideal technique to use for studying tip/surface interactions in STM.The elastic strain associated with tunnelling was first imaged on cleaved, highly doped (S doped, 5 × 1018cm-3) InP(110). The tip and surface damage observed provided strong evidence that the strain was caused by tip/surface contact, most likely through an insulating adsorbate layer. This is consistent with the picture that tunnelling in air, liquid or ordinary vacuum (such as in a TEM) occurs through a layer of contamination. The tip, under servo control, must compress the insulating contamination layer in order to get close enough to the sample to tunnel. The contaminant thereby transmits the stress to the sample. Elastic strain while tunnelling from graphite has been detected by others, but never directly imaged before. Recent results using the STM/REM combination has yielded the first direct evidence of strain while tunnelling from graphite. Figure 2 shows a graphite surface elastically strained by the STM tip while tunnelling (It=3nA, Vtip=−20mV). Video images of other graphite surfaces show a reversible strain feature following the tip as it is scanned. The elastic strain field is sometimes seen to extend hundreds of nanometers from the tip. Also commonly observed while tunnelling from graphite is an increase in the RHEED intensity of the scanned region (see fig.3). Debris is seen on the tip and along the left edges of the brightened scan region of figure 4, suggesting that tip abrasion of the surface has occurred. High resolution TEM images of other tips show what appear to be attached graphite flakes. The removal of contamination, possibly along with the top few layers of graphite, seems a likely explanation for the observed increase in RHEED reflectivity. These results are not inconsistent with the “sliding planes” model of tunnelling on graphite“. Here, it was proposed that the force due to the tunnelling probe acts over a large area, causing shear of the graphite planes when the tip is scanned. The tunneling current is then modulated as the planes of graphite slide in and out of registry. The possiblity of true vacuum tunnelling from the cleaned graphite surface has not been ruled out. STM work function measurements are needed to test this.

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The German Post-Critical Belief Scale: Internal and External Validity

Zeitschrift für Sozialpsychologie ◽

10.1024/0044-3514.34.4.219 ◽

2003 ◽

Vol 34 (4) ◽

pp. 219-226 ◽

Cited By ~ 7

Author(s):

Bart Duriez ◽

Claudia Appel ◽

Dirk Hutsebaut

Keyword(s):

Internal Structure ◽

Present Article ◽

External Validity ◽

German Version ◽

Dutch Version ◽

German Sample ◽

Belief Scale ◽

Internal And External Validity

Abstract: Recently, Duriez, Fontaine and Hutsebaut (2000) and Fontaine, Duriez, Luyten and Hutsebaut (2003) constructed the Post-Critical Belief Scale in order to measure the two religiosity dimensions along which Wulff (1991 , 1997 ) summarized the various possible approaches to religion: Exclusion vs. Inclusion of Transcendence and Literal vs. Symbolic. In the present article, the German version of this scale is presented. Results obtained in a heterogeneous German sample (N = 216) suggest that the internal structure of the German version fits the internal structure of the original Dutch version. Moreover, the observed relation between the Literal vs. Symbolic dimension and racism, which was in line with previous studies ( Duriez, in press ), supports the external validity of the German version.

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Internal Structure of the Spanish Adaptation of the Eating Disorder Inventory-3

European Journal of Psychological Assessment ◽

10.1027/1015-5759/a000087 ◽

2012 ◽

Vol 28 (1) ◽

pp. 25-31 ◽

Cited By ~ 7

Author(s):

Paula Elosua ◽

Alicia López-Jáuregui

Keyword(s):

Eating Disorders ◽

Eating Disorder ◽

Internal Structure ◽

Internal Consistency ◽

Clinical Sample ◽

Temporal Stability ◽

Second Order ◽

Order Structure ◽

Eating Disorder Inventory ◽

High Internal Consistency

In this study the Eating Disorder Inventory-3 was adapted to Spanish and analyzed the internal psychometric properties of the test in a clinical sample of females with eating disorders. The results showed a high internal consistency of the scores as well as high temporal stability. The factor structure of the scale composites was analyzed using confirmatory factor analysis. The results supported the existence of a second-order structure beyond the psychological composites. The second-order factor showed high correlation with the factor related to eating disorders. Overall, the Spanish version of the EDI-3 showed good psychometric qualities in terms of internal consistency, temporal stability and internal structure.

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