scholarly journals Isolation and Identification of Pathogenic Bacteria and Fungi from Some Saudi Bank Note Currency

2017 ◽  
Vol 14 (2) ◽  
pp. 715-720
Author(s):  
Abdulmoniem M. A. Saadabi ◽  
Mohammed S. Alhussaini ◽  
Abdullah A. Al-Ghanayem ◽  
Babu Joseph ◽  
Mohammed S. A-l Shuriam
Keyword(s):  
Pathogenic Bacteria ◽  
Pathogenic Fungi ◽  
Personal Hygiene ◽  
Isolation And Identification ◽  
Bank Note ◽  
Candida Sp ◽  
Alternaria Sp ◽  
Aspergillus Sp ◽  
Bacteria And Fungi ◽  
Bank Notes

ABSTRACT: Saudi Bank notes in different values were critically evaluated to show the level of contamination with pathogenic bacteria and fungi using standard techniques. The fungal genera Trichophyton sp., Microsporum sp., Epidermophyton sp., Aspergillus sp., Alternaria sp., Penicillium sp., Candida sp., Phycomyces sp., Saccharomyces sp. and Cladosporium sp. were isolated and identified. The genera of bacteria that isolated were Escherichia coli, Citrobacter sp., Klebsiella sp., Proteus sp., Streptococcus sp., Bacillus sp., Corynebacterium sp. and Staphylococcus sp. The potential of Saudi currency notes to act as environmental vehicles for transmission of pathogenic fungi & bacteria was assessed. The lower values of bank notes were highly contaminated and there is a negative correlation between bank notes value and microbial contamination. The results suggest that currency notes may be contaminated, especially with bacteria and enteric microbes and may serve as a source of contamination or infection. Personal hygiene and electronic or credit banking to reduce the risk of infection were recommended.

scholarly journals Isolation frequency of medically important fungi and fluconazole resistant of Candida sp from hospital cockroaches.

2020 ◽  
Author(s):  
Doreen Anna Mloka ◽  
George M Bwire ◽  
Kenndy Mwambete
Keyword(s):  
Fungal Pathogens ◽  
Isolation And Identification ◽  
Opportunistic Fungi ◽  
Candida Sp ◽  
Microdilution Method ◽  
Alternaria Sp ◽  
Fusarium Sp ◽  
Aspergillus Sp ◽  
Rhizopus Sp ◽  
Parasitic Pathogens

Abstract BackgroundCockroaches are common pests in homes and hospitals. They are known to cause allergic reactions in certain individuals and have found to be potential vectors for various bacterial and parasitic pathogens. This study assessed the potential of hospital cockroaches to act as vectors of medically important fungal pathogens on their external surfaces.MethodsCockroaches were captured from the main Intensive care unit (ICU), burn unit, adult surgical wards , pediatric oncology wards, intern hostel kitchen and the central kitchen of a national referral teaching hospital in Tanzania. Normal saline washings from the external surface of cockroaches were cultured on standard mycological media to facilitate isolation and identification of medically important molds and yeasts. Susceptibility of Candida sp isolates to fluconazole was tested using the CLSI M27-A3 microdilution method.ResultsAt total of 72 cockroaches were captured from various sites of the hospital between February and March 2015. All cockroaches captured were shown to carry medically importance fungi. A total of 956 medically important fungi were isolated, 57.9 % were Candida sp., 23.2%, Aspergillus sp. , 3.1% Cladosporium sp., 1.8% Rhizopus sp., 1.2 % Geotrichum sp., 0.9 % Pencillum sp. , 0.7% Alternaria sp, 0.6 % Fusarium sp , 0.3% Mucor sp and 10.1% others. Aspergillus fumigatus (50.0%) was the most commonly isolated followed by Aspergillus niger ( 15.8%) among the Aspergillus isolates.Over 16.3 % of the Candida isolates not intrinsically resistant to fluconazole showed resistance to this drug.mResistance was most frequently found in Candidapseudotropicalis (23.8%) and Candida glabarata (20.0%) and least in isolates of Candida albicans ( 6.3%). ConclusionThe external surfaces of cockroaches from this hospital may act as reservoirs of medically important opportunistic fungi exhibiting resistance to fluconazole.

scholarly journals Antimicrobial Activity and Toxicity of Sophorolipids Produced by Candida haemulonis and Saccharomyces cerevisiae against Some Selected Microorganisms

2021 ◽  
pp. 1-9
Author(s):  
K. F. Williams ◽  
O. K. Agwa ◽  
G. O. Abu ◽  
O. Akaranta
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acute Toxicity ◽  
Pathogenic Bacteria ◽  
Scale Up ◽  
Antagonistic Activity ◽  
Candida Sp ◽  
Test Organisms ◽  
Aspergillus Sp ◽  
Bacteria And Fungi ◽  
Rhizopus Sp

Sophorolipid have been identified to possess varying antagonism relationship to a number microbiota, although having been identified as being ecologically friendly. The biosafety of biologically synthesized materials has been identified as a major challenge to commercialization and scale-up. Sophorolipid was produced by Candida haemulonis and Saccharomyces cerevisiae in Palm oil mill effluent impacted soil in Emohua, Rivers State. Submerged fermentation was employed in the production of the sophorolipid while the Well-in-agar approach was employed in the antimicrobial susceptibility was conducted using 20%, 40%, 60%, 80% and 100% on both beneficial and pathogenic bacteria and fungi namely Staphylococcus sp, Nitrobacter sp, Klebsiella sp, Bacillus sp while the fungal flora were Aspergillus sp, Penicillium sp, Rhizopus sp, Candida sp, Mucor sp and Saccharomyces sp. Acute toxicity was conducted using Nitrobacter sp, Nitrosomonas sp and Thiobacillus sp. Probit based determination of acute toxicity after a 48hr and 96hr exposure to the test organisms. Antagonistic nature of the sophorolipids showed there was little or no antagonistic activity on the bacterial isolates than the fungal isolates. The sophorolipid formulation synthesized from both isolates were observed to have a zone of inhibition between 10 mm to 24 mm for Nitrosomonas sp. while to Aspergillus sp it had a range between 4.0 mm to 13.0 mm. Acute toxicity of sophorolipid produced by Candida haemulonis against Nitrosomonas sp 0.054 mg/L while to Thiobacillus sp it had an acute toxicity index of 0.107 mg/L.  This study identified that the sophorolipid produced by the yeast isolate had the potential of being employed in a number of sectors. This study has pioneered the untapped benefits in the POME-induced flora and how they can be veritable tools in the biotechnology industry.

Isolation and Identification of the Main Pathogenic Fungi from Postharvest Hami Melon in Later Period of Storage

2011 ◽  
Vol 343-344 ◽  
pp. 1212-1216 ◽  
Author(s):  
Cun Kun Chen ◽  
Wen Sheng Wang ◽  
Jia Ning ◽  
Yuan Hui Gao
Keyword(s):  
Bacterial Strain ◽  
Pathogenic Fungi ◽  
Isolation And Identification ◽  
Koch’S Postulates ◽  
Penicillium Sp ◽  
Alternaria Sp ◽  
Fusarium Sp ◽  
Koch's Postulates

In order to investigate the reasons for the occurrence of melon disease, according to Koch’s postulates, the pathogenic fungi which caused the disease of postharvest melon“86-1”has been isolated and three kinds of bacterial strain has been identified. By identifying the pathogenicity, results revealed that they are Alternaria sp., Fusarium sp. and Penicillium sp.. They are the major pathogenic fungi which caused the decay of the postharvest Hami melon.

scholarly journals Isolation and Identification of Antibiotic-Producing Halophilic Bacteria from Dagh Biarjmand and Haj Aligholi Salt Deserts, Iran

2019 ◽  
Vol 25 (1) ◽  
pp. 70-77 ◽  
Author(s):  
Babak Elyasifar ◽  
Sevda Jafari ◽  
Somayeh Hallaj-Nezhadi ◽  
Florence Chapeland-leclerc ◽  
Gwenaël Ruprich-Robert ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Plant Pathogens ◽  
Halophilic Bacteria ◽  
Pathogenic Fungi ◽  
Antibacterial Activities ◽  
Biochemical Properties ◽  
Diffusion Method ◽  
Human Pathogens ◽  
Isolation And Identification ◽  
Phylogenic Tree

Background: Halophilic bacteria are potent organisms in production of novel bioactive antimicrobial compounds which might be considered in drug innovation and control of plant pathogens. Salt deserts in Semnan province are of the most permanent hypersaline areas in the North of Iran. Despite the importance of these areas, there is no scientific report regarding the biodiversity and potency of their halophilic bacteria. Thus, aforementioned areas were selected to detect the halophilic bacteria. Methods: Here, seven strains were isolated and cultured on their molecular and biochemical properties were characterized. To determine the antibiotic potency of the isolates, agar well diffusion method was conducted. Phylogenetic analysis was done to reveal the isolates relationship with previously known strains. Results: As a result, growth of the strains in the medium containing 5 to 20% (w/v) NaCl determined that the majority of the isolates were moderately halophile. Catalase activity of all strains was positive. The results represented that D6A, Dar and D8B have antimicrobial effects against different plant and human pathogens. Phylogenic tree analysis also showed that two strains of D6A and Dar are belonged to Bacillus subtilis and D8B is belonged to Virgibacillus olivae. The bacteria extracts were evaluated for their antifungal and antibacterial activities on human and Plant pathogenic strains. The MIC of the extract B. subtilis against was found active against human pathogenic fungi and Plant pathogenic bacteria and fungi, ranging from 12.5 to 25 µg/mL. Conclusion: This study highlights the therapeutic and prophylactic potential of B. subtilis extracts as antibacterial and antifungal agents.

scholarly journals Isolation and Identification of Saccharomyces cerevisiae from Caterpillar Frass and their Probiotic Characterization

2019 ◽  
Vol 16 (1) ◽  
pp. 179-186 ◽  
Author(s):  
Ujwala Vinayak Khisti ◽  
Suyash Arun Kathade ◽  
Mayur Arjun Aswani ◽  
Pashmin Kaur Anand ◽  
Nirichan Kunchirman Bipinraj
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Yeast Cells ◽  
Isolation And Identification ◽  
Yeast Cultures ◽  
Isolation And Characterization ◽  
Wide Range ◽  
Probiotic Yeast ◽  
Bacteria And Fungi

Probiotics are live microorganisms which upon ingestion confer health benefits to the host and are widely applied for human and animal welfare. The present study reports the isolation of yeast cells from caterpillar frasses and its probiotic characterization. Out of four yeast cultures isolated, all found to be non-hemolytic and cultures designated as CV-I, CV-II CV-III and CV-IV showed good bile tolerance at 1.2%. These cultures possessed the ability to grow pH range of 1.5 – 10, exhibited auto-aggregation and co-aggregation capabilities, which are essential for growth in alimentary canal and reduction of pathogen adherence on the intestinal epithelial cells. All cultures exhibited good tolerance to temperature up to 42°C. Isolate CV-I showed wide range of antimicrobial activities against pathogenic bacteria and fungi. This study is the first report of isolation and characterization of probiotic yeast from caterpillar frass. The isolate CV-I has been identified as Saccharomyces cerevisiae by molecular methods. This culture is an ideal candidate for further probiotic exploration.

scholarly journals Bioactivity of pyocyanin of Pseudomonas aeruginosa clinical isolates against a variety of human pathogenic bacteria and fungi species

10.3823/812 ◽  
2017 ◽  
Vol 7 (3) ◽  
Author(s):  
Zahraa Jaafar Jameel ◽  
Anaam Fuad Hussain ◽  
Muthana Abdulkhader Al-Mahdawi ◽  
Nuha F. Abed Alkerim ◽  
Eman Sabah Abd Alrahman
Keyword(s):  
Antimicrobial Activity ◽  
Secondary Metabolites ◽  
Pathogenic Bacteria ◽  
Pathogenic Fungi ◽  
Blue Pigment ◽  
Candida Spp ◽  
Source Of Infection ◽  
Redox Active ◽  
Bacteria And Fungi ◽  
Human Infections

Pyocyanin is blue pigment redox active, secondary metabolites produced by P. aeruginosa. The present study investigated the bioactivity of pyocyanin against certain types of bacteria and fungi causing human infections Objectives: Pyocyanin is blue pigment redox active, secondary metabolites produced by P. aeruginosa. The current study deals with biosynthesis, purification and bioactivity of pyocyanin produced by P. aeruginosa. Design: Pyocyanin extraction was done by chloroform method and concentration was determined by multiplying the optical density at 520 nm by 17.072 expressed as µg/ml. Biological activity of pyocyanin was determined by well diffusion procedure. Results: According to the source of infection, results showed that P. aeruginosa were most common in ear infection (30%) followed by wounds (22%), burns (17%), urine (13%) and each in stool and diabetes (9%). In this study the high resistance of  P. aeruginosa  isolates to antibiotics were 19 (82.6 %) to piperacillin followed by 10(43.5%) to aztreonem, 8(34.8%) to meropenem, 6(26.1%) to amikacin, 5(21.7%) to ciprofloxacin then 2(8.7%) to cefotaxime. the urine isolate produced the largest amount of pyocyanin (15.894 µg/ml). pyaocyanin have antimicrobial activity against Pathogenic bacteria: Shigella,  Staphyllococcus aureus and  Staphyllococcus epidermedis. and pathogenic fungi and yeast: Aspergillus niger, Penicillium spp., Rhizopus spp, Trichophyton  mentagrophyte, Rhodotorula spp., Alternaria alternate , Trichophyton rubrum and Candida spp Conclusions: cefotaxime is the best antibiotic for P. aeruginosa. Antimicrobial activity of pyocyanin against gram positive more than gram negative bacteria but less than that observed against fungi (molds and yeast).

scholarly journals Diversity of fungi obtained from bats captured in urban forest fragments in Sinop, Mato Grosso, Brazil

2023 ◽  
Vol 83 ◽  
Author(s):  
L. Ludwig ◽  
J. Y. Muraoka ◽  
C. Bonacorsi ◽  
F. C. Donofrio
Keyword(s):  
Urban Forest ◽  
Opportunistic Infections ◽  
Fungal Infections ◽  
Pathogenic Fungi ◽  
Domestic Animals ◽  
Forest Fragments ◽  
Pathogenic Potential ◽  
Mato Grosso ◽  
Candida Sp ◽  
Aspergillus Sp

Abstract Bats are important for the homeostasis of ecosystems and serve as hosts of various microorganisms including bacteria, viruses, and fungi with pathogenic potential. This study aimed to isolate fungi from biological samples obtained from bats captured in the city of Sinop (state of Mato Grosso, Brazil), where large areas of deforestation exist due to urbanization and agriculture. On the basis of the flow of people and domestic animals, 48 bats were captured in eleven urban forest fragments. The samples were processed and submitted to microbiological cultures, to isolate and to identify the fungal genera. Thirty-four (70.83%) of the captured bats were positive for fungi; 18 (37.5%) and 16 (33.33%) of these bats were female and male, respectively. Penicillium sp., Scopulariopsis sp., Fusarium sp., Aspergillus sp., Alternaria sp., Cryptococcus sp., Trichosporon sp., and Candida sp., which may cause opportunistic infections, were isolated. The bat species with the highest number of fungal isolates was Molossus molossus: 21 isolates (43.8%). According to our results, bats captured in urban forest fragments in Sinop harbor pathogenic fungi, increasing the risk of opportunistic fungal infections in humans and domestic animals.

scholarly journals Isolation and Characterization of Two New Metabolites from the Sponge-Derived Fungus Aspergillus sp. LS34 by OSMAC Approach

Marine Drugs ◽  
2019 ◽  
Vol 17 (5) ◽  
pp. 283 ◽  
Author(s):  
Wei Li ◽  
Lijian Ding ◽  
Ning Wang ◽  
Jianzhou Xu ◽  
Weiyan Zhang ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
K562 Cells ◽  
Isolation And Identification ◽  
Strong Activity ◽  
Potato Dextrose Broth ◽  
Isolation And Characterization ◽  
Growth Inhibitory Activity ◽  
Ic50 Values ◽  
Rice Medium ◽  
Aspergillus Sp

The application of an OSMAC (One Strain-Many Compounds) approach on the sponge-derived fungus Aspergillus sp. LS34, using two different media including solid rice medium and potato dextrose broth (PDB) resulted in the isolation and identification of two new compounds, named asperspin A (1) and asperther A (2) along with seven known compounds 3–9. Compounds 1–5 were detected in fungal extracts from rice medium, while compounds 6–9 were isolated from PDB medium. Their structures were unambiguously characterized by HRESIMS and NMR spectroscopic data. The growth inhibitory activity of these compounds against four pathogenic bacteria (Vibrio parahaemolyticus, Vibrio harveyi, Escherichia coli, and Staphylococcus aureus) were evaluated. All the compounds were also tested for their cytotoxicity against seven cancer cell lines, including CCRF-CEM, K562, BGC823, AGS, HCT-116, MDA-MB-453, and COR-L23. Among them, compound 9 showed strong activity against CCRF-CEM and K562 cells with IC50 values of 1.22 ± 0.05 µM and 10.58 ± 0.19 µM, respectively. Notably, compound 7 also showed pronounced activity against S. aureus with an MIC value of 3.54 µM.

Prevalence and Antimicrobial Susceptibility of Potentially Pathogenic Bacteria and Fungi Isolated from Public Parks of Bogotá, Colombia

2018 ◽  
Vol 21 (2) ◽  
pp. 85
Author(s):  
Diego Fernando González Lozano ◽  
Camila Andrea Camacho Ramos ◽  
Iván Alberto Méndez Rodríguez
Keyword(s):  
Salmonella Enteritidis ◽  
Pathogenic Bacteria ◽  
Public Health Problem ◽  
Personal Hygiene ◽  
Antibiotics Resistance ◽  
Pathogenic Species ◽  
Public Parks ◽  
Opportunistic Pathogens ◽  
Environmental Material ◽  
Bacteria And Fungi

Regular interactions of humans with their environment, animals and different microorganisms as part of everyday routine can cause several infections or diseases that can become a public health problem if these are not properly controlled. It is necessary to identify potentially pathogenic bacteria (with its antimicrobial resistance) and fungi, and their prevalence in public parks in Bogotá city. Four parks were evaluated, where samples were taken from dog’s feces and hair, recreational items and environmental material, to perform the microorganism’s isolations. The higher prevalence percentages in each sort of sample were for Escherichia coli, Staphylococcus epidermidis, Staphylococcus aureus, Rahnella aquatilis, Penicillium spp, Cladosporium spp. and Mucor spp. and there were pathogenic species like Salmonella enteritidis and Klebsiella pneumoniae. We report a strain of S. aureus with intermediate resistance to vancomycin (VISA) found in recreational items. The higher rates of antibiotics resistance like ampicillin and trimethoprim were found in the isolations from dog’s feces. Some species or genera reported are considerated as opportunistic pathogens; however, these and the pathogenic species reported represent a threat to human health and this is why it is essential to have a personal hygiene plan after stay at a park.

Sign in / Sign up

Export Citation Format

Share Document