scholarly journals Biogenic Synthesis of Bi-Metallic (Zn-Cu) Nanoparticles by Leaf Extract of Citrus Limon and Evaluation of its Antibiofilm Activity Against E. coli

2021 ◽  
Vol 14 (4) ◽  
pp. 2017-2028
Author(s):  
Tooba Mazhar ◽  
Vikas Shrivastava ◽  
Rajesh Singh Tomar
Keyword(s):  
Metallic Nanoparticles ◽  
Bimetallic Nanoparticles ◽  
Average Particle Size ◽  
Antibacterial Properties ◽  
Colour Change ◽  
Antibiofilm Activity ◽  
Citrus Limon ◽  
Average Particle ◽  
Cu Nanoparticles ◽  
E Coli

The rapid hike in the usage of metallic as well as non-metallic nanoparticles demands their increased synthesis. In our study we synthesized bimetallic Zn-Cu nanoparticles using the greener route i.e. dry leaves powder of Citrus limon as it is environment-friendly, cost-effective, has a high surface area to volume ratio and hence superior over monometallic nanoparticles synthesized via physical or chemical approach. UTI is the most common nosocomial infection and the bacteria associated with it usually is E. coli. This pathogen forms biofilm and makes it difficult to treat the disease in less time. Hence, antimicrobial as well as antibiofilm activity of synthesized bimetallic nanoparticles was checked against E. coli. Our experimental procedure involved the preparation of ethanolic plant extract using dry leaf powder followed by synthesis of bimetallic nanoparticles. Particles were then characterized by using biophysical techniques such as FTIR, Powder-XRD and SEM-EDX. Next, we identified bacteria isolated from environment and hospital source and prepared their pure cultures. Lastly, we carried out the antibacterial and antibiofilm activity of synthesized nanoparticles against isolated E. coli. Particles showed the colour change from blue to green upon synthesis and were found to be a triclinic primitive type with an average particle size calculated to be 27.76nm as seen in PXRD. FTIR analysis gave characteristic peaks of functional groups. SEM-EDX confirmed successful doping and grain size of the particle. Bacteria isolated from samples showed pink rods in gram staining indicating gram-negative bacilli. Biochemical findings confirmed E. coli in samples. Characteristic zones of inhibition in range 12-18mm establish good antibacterial properties with MIC of synthesized nanoparticles <0.5mg/ml and crystal violet assay assure antibiofilm properties of Zn-Cu. The result of the study can be advantageous to develop an understanding of the development of nano-based medicine for biofilm-producing pathogens.

scholarly journals Decoding Antibacterial and Antibiofilm Properties of Cinnamon and Cardamom Essential Oils: A Combined Molecular Docking and Experimental Study

2021 ◽  
Author(s):  
Elahe Pourkhosravani ◽  
fatemeh dehghan nayeri ◽  
Mitra Mohammadi Bazargani
Keyword(s):  
Cell Attachment ◽  
Antibacterial Properties ◽  
Binding Mode ◽  
Dilution Method ◽  
Antibiofilm Activity ◽  
Bacterial Strains ◽  
Biofilm Growth ◽  
Computational Docking ◽  
E Coli ◽  
Industrial Strains

Abstract This study sets out to compare the antibacterial and antibiofilm profiles of Ci/Ca EOs alone and in combination together against infectious bacterial strains. MIC assay was carried out to survey the effectiveness of prepared EOs by two-fold serial dilution method and MTT evaluation. Synergic antibacterial properties of EOs against target strains were studied by using checkerboard titration method. Biofilm growth and development were evaluated using CV and XTT reduction assays. Antibacterial activity was observed for EOs against both bacterial strains with stronger activity for CiEO against both bacteria. The synergistic antibacterial effect was observed only against B. subtilis. Based on the FIC index, combinations could not inhibit the growth of E. coli. The pure EOs and their combination inhibited cell attachment for both studied bacteria with stronger effect on E. coli. CV and XTT reduction assays results showed that Ci EO and its combination with CaEO had the highest antibiofilm activity at lowest MIC value 0.08% and 0.04/0.02% against biofilm formed by E. coli and B. subtilis respectively, indicating a high antibiofilm potential. Computational docking analyses also postulated that the active constituents of evaluated EOs have the potential to interact with different bacterial targets, suggested binding mode of action of EOs metabolites. By and large, synergistic anti-biofilm properties of EOs may provide further options for developing novel formula to inhibit a variety of infectious clinical and industrial strains without (or less) toxicity effects on human body.

ULTRAFINE CONTROL OF Cu NANOPARTICLES AND THEIR ANTIBIOTIC EFFECT ON E. coli

2019 ◽  
Vol 26 (07) ◽  
pp. 1850214
Author(s):  
QINGBIN WANG ◽  
LIANGYUAN SHI ◽  
YUN YANG ◽  
HUI HUANG ◽  
YI JIE ◽  
...  
Keyword(s):  
Metallic Nanoparticles ◽  
Size Control ◽  
Cu Nanoparticles ◽  
Electron Microscopic ◽  
Tem Analysis ◽  
Cluster Technique ◽  
E Coli ◽  
Transmission Electron ◽  
Antibiotic Effect ◽  
Potential Applications

Metallic nanoparticles have attracted intense interest for the potential applications in biocompatibility due to the reduced particle size. However, the methods to produce metallic nanoparticles usually produce an inhomogeneous size distribution. In this work, Cu nanoparticles were generated using a gas-aggregation cluster source technique, employing a specially designed quadrupole mass filter to control the size of the nanoparticles with a mass resolution (m/[Formula: see text]m) of 5. Transmission electron microscopic (TEM) analysis was used to confirm the size control of our technique. The generally high angular electronic scattering analysis revealed the spherical shapes of the Cu nanoparticles. We used beams of these nanoparticles to prepare nano-granular films on a Si substrate. Their antibacterial effect of the modified materials on Escherichia coli was assessed by means of a bacterial adhesion test. Our results may not only reveal the cluster technique to produce the uniform metallic nanoparticles, but also form the basis of antibacterial applications.

Ni/MWCNT-Supported Palladium Nanoparticles as Magnetic Catalysts for Selective Oxidation of Benzyl Alcohol

2013 ◽  
Vol 66 (5) ◽  
pp. 564 ◽  
Author(s):  
Mingmei Zhang ◽  
Qian Sun ◽  
Zaoxue Yan ◽  
Junjie Jing ◽  
Wei Wei ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Benzyl Alcohol ◽  
Bimetallic Nanoparticles ◽  
Average Particle Size ◽  
Average Particle ◽  
X Ray ◽  
Oxidation Of Benzyl Alcohol ◽  
Uniform Dispersion ◽  
Multi Walled Carbon Nanotubes ◽  
Walled Carbon Nanotubes

Well dispersed Pd@Ni bimetallic nanoparticles on multi-walled carbon nanotubes (Pd@Ni/MWCNT) are prepared and used as catalysts for the oxidation of benzyl alcohol. Scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy analysis, and X-ray diffraction were performed to characterise the synthesised catalyst. The results show a uniform dispersion of Pd@Ni nanoparticles on MWCNT with an average particle size of 4.0 nm. The as synthesised catalyst was applied to the oxidation of benzyl alcohol. A 99 % conversion of benzyl alcohol and a 98 % selectivity of benzaldehyde were achieved by using the Pd@Ni/MWCNT (Pd: 0.2 mmol) catalyst with water as a solvent and H2O2 as oxidant at 80°C. The catalytic activity of Pd@Ni/MWCNT towards benzyl alcohol is higher than that of a Pd/MWCNT catalyst at the same Pd loadings. The catalyst can be easily separated due to its magnetic properties.

Dual synthesis of Silver and Iron oxide nanoparticles from edible greens Amaranthus Viridis and their in vitro antioxidant activity and antimicrobial studies

2021 ◽  
Vol 10 ◽  
Author(s):  
Venkata Subbaiah Kotakadi ◽  
Bhulakshmi Kolapalli ◽  
Susmila Aparna Gaddam ◽  
Sai Gopal Divi Venkata Ramana
Keyword(s):  
Antioxidant Activity ◽  
Particle Size ◽  
Green Synthesis ◽  
Metallic Nanoparticles ◽  
Iron Nanoparticles ◽  
Average Particle Size ◽  
Leafy Vegetable ◽  
Size Analysis ◽  
Average Particle

Background: There is an increasing commercial demand for nanoparticles due to their wide applicability in various areas such as chemistry, catalysis, energy and medicine. Metallic nanoparticles are traditionally synthesized by wet chemical techniques where the chemicals used are quite often toxic and flammable. Objective: In the present study, we described a simple, cost effective and environmentally-friendly technique for green synthesis of silver and iron nanoparticles by using the aqueous extract of leafy vegetable Amaranthus viridis as a reducing agent. Methods: The silver and Iron nanoparticles (Av-AgNPs, Av-IONPs) were characterized by different spectral methods. The surface Plasmon resonance spectrums of Av-AgNPs, Av-IONPs were recorded at 422nm and 261nm. The Scanning electron microscopy (SEM) analysis reveals that the Av-AgNPs, Av-IONPs are roughly spherical in shape. Energy dispersive absorption spectroscopy (EDAX) of biosynthesized Av-AgNPs, Av-IONPs indicates the reduction of silver ions to elemental silver and iron ions to elemental iron. Results: The particle size analysis of Av-AgNPs and Av-IONPs was carried out by Dynamic light scattering (DLS) method the results reveal that both Av-AgNPs and Av-IONPs were polydispered in nature. The average particle size of Av-AgNPs is 55.8 nm with a polydispered index (PI) of 0.297, similarly the average particle size of Av-IONPs is 80.6 nm with an polydispered index (PI) of 0.469. Zeta-potential of Av-AgNPs was detected at -24.6 mV and Av-IONPs were detected at 28.8 mV, the result reveals that they high stability due their high negative charge and positive charge respectively. The dual synthesized Av-AgNPs, Av-IONPs exhibits excellent antioxidant activity by DPPH, H2O2 and NO methods. DPPH was proven to be the best when compared with the other two methods. The biosynthesized Av-AgNPs, Av-IONPs proved to have very good antimicrobial activity against gram +ve and gram –ve bacteria. Conclusion: when compared with standard antibiotic. There were several reports on green synthesis of metal nanoparticles using various plant parts, but here edible leafy vegetable Amaranthus viridis was used for biosynthesis of both Av-AgNPs and Av-IONPs.

scholarly journals Considerable Variation of Antibacterial Activity of Cu Nanoparticles Suspensions Depending on the Storage Time, Dispersive Medium, and Particle Sizes

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Olga V. Zakharova ◽  
Anna Yu. Godymchuk ◽  
Alexander A. Gusev ◽  
Svyatoslav I. Gulchenko ◽  
Inna A. Vasyukova ◽  
...  
Keyword(s):  
Storage Time ◽  
Antibacterial Properties ◽  
Physiological Solution ◽  
Cu Nanoparticles ◽  
E Coli ◽  
New Class ◽  
Low Concentrations ◽  
Nanoparticle Suspensions ◽  
Sodium Dichloroisocyanurate ◽  
Average Size

Suspensions of Cu nanoparticles are promising for creating the new class of alternative antimicrobial products. In this study we examined copper nanoparticles of various sizes obtained by the method of wire electric explosion: nanopowder average size 50 nm (Cu 50) and 100 nm (Cu 100). The paper presents the complex study of the influence of physicochemical properties such as particle size and concentration of the freshly prepared and 24-hour suspensions of Cu nanoparticles in distilled water and physiological solution upon their toxicity to bacteriaE. coliM-17. Ionic solution of Cu2+and sodium dichloroisocyanurate was used for comparison study. It has been shown that decrease in the nanoparticle size leads to changes in the correlation between toxicity and concentration as toxicity peaks are observed at low concentrations (0.0001⋯0.01 mg/L). It has been observed that antibacterial properties of Cu 50 nanoparticle suspensions are ceased after 24-hour storage, while for Cu 100 suspensions no correlation between antibacterial properties and storage time has been noted. Cu 100 nanoparticle suspensions at 10 mg/L concentration display higher toxicity at substituting physiological solution for water than Cu 50 suspensions. Dependence of the toxicity on the mean particle aggregates size in suspension was not revealed.

scholarly journals Antibacterial Shoe Insole-Coated CuO-ZnO Nanocomposite Synthesized by the Sol-Gel Technique

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Nguyen Lam Uyen Vo ◽  
Thi Thuy Van Nguyen ◽  
Tri Nguyen ◽  
Phung Anh Nguyen ◽  
Van Minh Nguyen ◽  
...  
Keyword(s):  
Inhibitory Concentration ◽  
Antibacterial Effect ◽  
Sol Gel ◽  
Average Particle Size ◽  
Point Of Zero Charge ◽  
Average Particle ◽  
E Coli ◽  
High Antibacterial Activity ◽  
Gel Technique ◽  
Shoe Insoles

In this study, CuO-ZnO composite was synthesized via the sol-gel method using oxalic acid to form the medium complex and its applications in antibacterial have been conducted with B. cereus, E. coli, S. aureus, Salmonella, and P. aeruginosa. Then, nanopowder of CuO-ZnO was coated on shoe insoles and their antibacterial effect with S. aureus was tested. The nanocomposite products were characterized by XRD, XPS, SEM, TEM, and UV-Vis. The results showed that the CuO-ZnO composite has the average particle size in a range of 20-50 nm, the point of zero charge of 7.8, and the bandgap of 1.7 eV. XPS result shows the composite structure with Cu2+ in the product. The minimum inhibitory concentration (MIC) of CuO-ZnO nanocomposite was 0.313 mg·mL-1 for S. aureus and Samonella, 0.625 mg·mL-1 for E. coli, and 5 mg·mL-1 for B. cereus and P. aeruginosa. The shoe insoles coated with 0.35 wt.% of CuO-ZnO nanocomposite also had high antibacterial activity against S. aureus, and this antibacterial nanocomposite was implanted durably on the surface of the shoe insoles.

scholarly journals Biosynthesis and Characterization of Silver Nanoparticles from Bitter Melon (Momordica charantia) Fruit and Seed Extract and their Antimicrobial Activity

2021 ◽  
Vol 2 (1) ◽  
pp. 1-11
Author(s):  
Umme Ruman ◽  
Poonah Kia
Keyword(s):  
Silver Nanoparticles ◽  
Average Particle Size ◽  
Antibacterial Properties ◽  
Seed Extract ◽  
Food Preservation ◽  
Momordica Charantia ◽  
Average Particle ◽  
Ag Nps ◽  
Fruit Extract ◽  
Biotechnological Approach

Momordica charantia is a phenolic rich vegetable. In this study, the fruits and seeds extract of M. charantia were used to synthesize silver nanoparticles (Ag NPs) using biotechnological approach. Structural, morphological, and antimicrobial properties of the synthesized Ag-NPs were characterized using UV/Vis Spectrophotometry, Dynamic Light Scattering (DLS), High Resolution Transmission Electronics Microscopy (HRTEM), Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform Infrared Spectroscopy (FTIR) and X-Ray diffraction (XRD). In DLS, the average particle size of Ag-NPs was found 17.5 ± 2.1 nm and 18.3 ± 1.9 nm using seed and fruit extract, respectively. HRTEM has revealed their spherical structure for both seed and fruit extract of M. charantia. FESEM images found Ag-NPs with the size between ~20 and ~35 nm. The Ag NPs exhibited Surface Plasmon Resonance (SPR) centered at 405 nm for seed extract and 402 nm for fruit extract using a UV–visible spectrophotometer. FT-IR results showed phenolic and carbohydrate compounds involved in the synthesis of the Ag NPs. Furthermore, the synthesized Ag NPs has found highly rich in antibacterial properties against Escherichia coli and Pseudomonas aeruginosa bacterium. Thus, bioconversion of Ag NPs by M. charantia could be employed as a potential antibacterial source to eliminate pathogenic microorganisms from agricultural and food preservation industry.

scholarly journals Biosynthesis of Gold Nanoparticles by Essential Oil of Diplotaxis Acris Characterization and Antimicrobial Activities

2021 ◽  
Vol 37 (2) ◽  
pp. 405-412
Author(s):  
Mohamed Habib Oueslati ◽  
Lotfi Ben Tahar ◽  
A. Khuzaim Alzahrani ◽  
Jamith Basha ◽  
Omar H. Abd Elkader
Keyword(s):  
Essential Oil ◽  
Average Particle Size ◽  
Antimicrobial Activities ◽  
Nano Particles ◽  
Average Particle ◽  
Tem Analysis ◽  
Stabilizing Agents ◽  
E Coli ◽  
Ft Ir ◽  
Plausible Scheme

The present work reports a green biosynthesis of gold nano particles (EO-AuNPs) using an essential oil (EO) as a reducing agent of the Au(III) in HAuCl4. The EO was extracted by hydro-distillation from Diplotaxis acris flowers. A total of 16 compounds were detected from the EO oil by using GC–MS and 5-methylsulfanylpentanenitrile was identified as the major component (73.60 %). The biosynthesized EO-AuNPs were characterized performing UV–Vis, IR,XRD and TEM analyses.The UV-Vis revealed the typical features of surface plasmon resonance (SPR) of AuNPs at ~526 nm. The FT-IR spectrum of the biosynthesized nano particles exhibited the features of the nitrile (-C≡N) functional group indicating that the -C≡N-bearing EO components are likely acting as reducing and stabilizing agents for the formation of EO-AuNPs. The plausible scheme of EO-AuNPsformation was proposed.The TEM analysis showed that the EO- AuNPs were almost spherical in shape with an average particle size of 12.7 nm. In addition, the antimicrobial activity was carried out by diffusion of agar wells method. The results proved that the EO-AuNPs displayed a potential antimicrobial against gram negative strains, with a maximum zone of inhibition of 16 mm for E. coli at a concentration of 100 µg / ml.

scholarly journals Ordered intermetallic Pt–Cu nanoparticles for the catalytic CO oxidation reaction

RSC Advances ◽  
2016 ◽  
Vol 6 (88) ◽  
pp. 85634-85642 ◽  
Author(s):  
Govindachetty Saravanan ◽  
Rohini Khobragade ◽  
Laxmi Chand Nagar ◽  
Nitin Labhsetwar
Keyword(s):  
Particle Size ◽  
Co Oxidation ◽  
Oxidation Reaction ◽  
Average Particle Size ◽  
Pt Nanoparticles ◽  
Average Particle ◽  
Cu Nanoparticles ◽  
Catalytic Co Oxidation ◽  
Co Oxidation Reaction ◽  
Ordered Intermetallic

Intermetallic platinum (Pt) nanoparticles using the abundantly available element copper (Pt3Cu, PtCu, PtCu3) with an average particle size of 4–5 nm on a γ-Al2O3 support were prepared to reduce the consumption of Pt for the removal of CO from gases.

scholarly journals Modeling and Synthesis of Ag and Ag/Ni Allied Bimetallic Nanoparticles by Green Method: Optical and Biological Properties

2018 ◽  
Vol 2018 ◽  
pp. 1-17 ◽  
Author(s):  
Anuoluwa Abimbola Akinsiku ◽  
Enock Olugbenga Dare ◽  
Kolawole Oluseyi Ajanaku ◽  
Olayinka Oyewale Ajani ◽  
Joseph Adebisi O. Olugbuyiro ◽  
...  
Keyword(s):  
Environmental Remediation ◽  
Metallic Nanoparticles ◽  
Bimetallic Nanoparticles ◽  
Biological Properties ◽  
Optical Measurements ◽  
Ag Nps ◽  
Green Method ◽  
E Coli ◽  
Synthetic Routes ◽  
The Relationship

In the quest for environmental remediation which involves eco-friendly synthetic routes, we herein report synthesis and modeling of silver nanoparticles (Ag NPs) and silver/nickel allied bimetallic nanoparticles (Ag/Ni NPs) using plant-extract reduction method. Secondary metabolites in the leaf extract of Canna indica acted as reducing agent. Electronic transitions resulted in emergence of surface plasmon resonance in the regions of 416 nm (Ag NPs) and 421 nm (Ag/Ni NPs) during optical measurements. Further characterizations were done using TEM and EDX. Antimicrobial activity of the nanoparticles against clinical isolates was highly significant as P<0.05. These findings suggest application of Ag NPs as antibacterial agent against E. coli, S. pyogenes, and antifungal agent against C. albicans. Possible antibacterial drugs against S. pyogenes and E. coli can also be designed using Ag/Ni nanohybrid based on their strong inhibition activities. Similarly, the enhanced SPR in the nanoparticles is suggested for applications in optical materials, as good absorbers and scatters of visible light. Theoretical model clarified that the experiment observation on the relationship between metallic nanoparticles penetration through peptidoglycan layers and the activeness of microbial species depends on the nature of the nanoparticles and pore size of the layer.

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