Extraction of Bioactive Polyphenols with High Antioxidant Activity from Bilberry (Vaccinium myrtillus L.) Processing Waste

Fruits of bilberry (Vaccinium myrtillus L.) are valued mainly for their nutraceutical properties, and are among the fruits with the highest antioxidant activity due to their high content of phenolic compounds. The aim of this research was to assess the total polyphenol content and antioxidant activity of fruits in six wild bilberry populations from two regions of Romania over three years. The total polyphenol content was determined according to the Folin–Ciocalteu modified method, while the antioxidant activity was evaluated using the DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay. The Padis and Raul Lung populations registered the highest values of polyphenol content and antioxidant activity, as such the fruits of these bilberry populations could be considered potential sources of antioxidants for direct consumption or for use as ingredients for food products or food supplements. Significant variation of total polyphenol content and antioxidant activity was observed both between populations from the same region and from different regions. The low level of broad sense heritability for total polyphenol content and antioxidant activity associated with the high effects of year, and population–year interaction indicates that the accumulation of polyphenols in bilberry fruits is influenced by changes in environmental conditions.

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COMPARATIVE STUDY OF THE INFLUENCE OF ULTRASONIC INFLUENCES ON THE EXTRACTION OF ANTI-OXIDANT COMPOUNDS OF BLACKBERRY BERRIES (VACCINIUM MYRTILLUS L.)

chemistry of plant raw material ◽

10.14258/jcprm.2020014425 ◽

2020 ◽

pp. 167-177

Author(s):

Nadezhda Viktorovna Makarova ◽

Natal'ya Borisovna Yeremeyeva

Keyword(s):

Antioxidant Activity ◽

Antioxidant Properties ◽

Vaccinium Myrtillus ◽

Ultrasonic Extraction ◽

Biologically Active ◽

Extraction Technology ◽

High Biological Activity ◽

Plant Materials ◽

Dpph Method ◽

Vaccinium Myrtillus L

The interest in berries from food manufacturers is huge, which is explained by good organoleptic characteristics and high biological activity of berries. One way to preserve the properties of berries throughout the year is to obtain extracts. Determining the conditions and type of optimal extraction technology to obtain biologically active substances from plant materials is an important stage in the production of new natural preventive additives. The aim of this work is to study the chemical composition and antioxidant activity of blueberry extracts (Vaccinium myrtillus L.) obtained by various methods: maceration, ultrasonic and microwave extraction. The use of ultrasonic extraction as a method of intensifying the process of extracting biologically active compounds from blueberries is very effective. This proves an almost 2-fold increase in the flavonoid content in the extracts. However, the content of anthocyanins in the extracts of blueberries is not the highest for ultrasonic extraction (a decrease by 3.3 times compared with maceration), in all likelihood due to the instability of this class of compounds in this type of processing. Among the extracts of blueberries obtained by three technologies (maceration, microwave and ultrasound treatment), it is the ultrasound extract that has the highest antioxidant activity (9.5±0.1 mg/cm3, 18.18 mmol±0.24 Fe2+/1 kg, 58.6%), determined by three methods: DPPH method, FRAP method, method for assessing antioxidant properties using a model system with linoleic acid. Ultrasonic extraction was chosen as a technology for obtaining a concentrated blueberry extract.

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PHENOLIC AND ANTHOCYANIN CONTENT AND ANTIOXIDANT ACTIVITY IN FRUITS OF BILBERRY (VACCINIUM MYRTILLUS L.) AND OF HIGHBUSH BLUEBERRY (V. CORYMBOSUM L.) CULTIVARS IN NORTH WESTERN ITALY

Acta Horticulturae ◽

10.17660/actahortic.2006.715.85 ◽

2006 ◽

pp. 553-558 ◽

Cited By ~ 13

Author(s):

G. Beccaro ◽

M.G. Mellano ◽

R. Botta ◽

V. Chiabrando ◽

G. Bounous

Keyword(s):

Antioxidant Activity ◽

Vaccinium Myrtillus ◽

Highbush Blueberry ◽

Anthocyanin Content ◽

Vaccinium Myrtillus L ◽

North Western

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Chemical Composition and Biological Activities of the Nord-West Romanian Wild Bilberry (Vaccinium myrtillus L.) and Lingonberry (Vaccinium vitis-idaea L.) Leaves

Antioxidants ◽

10.3390/antiox9060495 ◽

2020 ◽

Vol 9 (6) ◽

pp. 495 ◽

Cited By ~ 2

Author(s):

Bianca-Eugenia Ștefănescu ◽

Lavinia Florina Călinoiu ◽

Floricuța Ranga ◽

Florinela Fetea ◽

Andrei Mocan ◽

...

Keyword(s):

Antioxidant Activity ◽

Plant Material ◽

Biological Activities ◽

Natural Habitat ◽

Vaccinium Myrtillus ◽

Bacterial Strains ◽

Leaf Extracts ◽

Natural Habitats ◽

Vaccinium Myrtillus L

This study was performed to evaluate and compare the in vitro antioxidant, antimicrobial, and antimutagenic activities, and the polyphenolic content of the Nord-West Romanian wild bilberry (Vaccinium myrtillus L.) and lingonberry (Vaccinium vitis-idaea L.) leaves from three different natural habitats (Smida, Turda, Borsa). In the case of both species, the flavanols level was higher in Smida habitat (altitude 1100 m), whereas quercetin derivates were more abundant in Borsa habitat (altitude 850 m). The bilberry leaf extracts contained in the highest amounts the feruloylquinic acid (59.65 ± 0.44 mg/g for Borsa habitat) and rutin (49.83 ± 0.63 mg/g for Borsa habitat), and showed relevant 2,2-diphenyl-1-picrylhydrazyl (DPPH) antioxidant activity (271.65 mM Trolox/100 g plant material for Borsa habitat, 262.77 mM Trolox/100 g plant material for Smida habitat, and 320.83 mM Trolox/100 g plant material for Turda habitat), for all the three extracts. Gallocatechin was the dominant flavanol in lingonberry species, with the highest amount being registered for Smida habitat (46.81 ± 0.3 mg/g), revealing a DPPH antioxidant activity of 251.49 mM Trolox/100 g plant material. The results obtained in the antimicrobial tests showed that the best inhibitory effect among bilberry species was attributed to the Turda (altitude 436 m) and Smida locations, against both Gram-positive and Gram-negative bacterial strains. For lingonberry, the differences in habitat did not influence the antibacterial effect, but the antifungal effect, only in the case of Candida zeylanoides. A strong antimutagenic effect was registered by the bilberry leaves toward Salmonella typhimurium TA100. Our study may be able to provide a better understanding of the correlation between natural habitat conditions and the accumulation of secondary metabolites and their related bioactivities in studied leaves.

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Seasonal variations of the phenolic constituents in bilberry ( Vaccinium myrtillus L.) leaves, stems and fruits, and their antioxidant activity

Food Chemistry ◽

10.1016/j.foodchem.2016.06.042 ◽

2016 ◽

Vol 213 ◽

pp. 58-68 ◽

Cited By ~ 29

Author(s):

Oana-Crina Bujor ◽

Carine Le Bourvellec ◽

Irina Volf ◽

Valentin I. Popa ◽

Claire Dufour

Keyword(s):

Antioxidant Activity ◽

Seasonal Variations ◽

Vaccinium Myrtillus ◽

Phenolic Constituents ◽

Vaccinium Myrtillus L

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Bilberry (Vaccinium myrtillus L.) Extracts Comparative Analysis Regarding Their Phytonutrient Profiles, Antioxidant Capacity along with the In Vivo Rescue Effects Tested on a Drosophila melanogaster High-Sugar Diet Model

Antioxidants ◽

10.3390/antiox9111067 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1067 ◽

Cited By ~ 1

Author(s):

Andreea-Adriana Neamtu ◽

Rita Szoke-Kovacs ◽

Emoke Mihok ◽

Cecilia Georgescu ◽

Violeta Turcus ◽

...

Keyword(s):

Antioxidant Activity ◽

Drosophila Melanogaster ◽

Total Content ◽

Vaccinium Myrtillus ◽

Anthocyanin Content ◽

Fruit Extract ◽

High Sugar ◽

Bilberry Extract ◽

Vaccinium Myrtillus L

Bilberries (Vaccinium myrtillus L.) have been reported to hold a plentitude of health-promoting properties beyond basic nutrition, mainly attributed to their anthocyanin content and antioxidant activity. In this article, we built the phytochemical profile of three wild bilberry fruit extract formulations (aqueous, methanolic, and hydro-methanolic) using UHPLC-ESI-MS/MS putative analysis, identifying 88 individual phytochemicals, mainly flavonoids (total content 8.41 ± 0.11 mg QE/g dw), free amino acids, polyphenols (total content 21.68 ± 0.19 mg GAE/g dw), carboxylic acids, and vitamins. Furthermore, the antioxidant activity of the extract was assessed, reaching 78.03 ± 0.16% DPPH free radical scavenging activity, comparable to literature values determined for bilberry extracts of other origin. Due to the increased prevalence of metabolic syndrome and based on the reviewed benefits of bilberries, we tested the most potent formulation of our bilberry extracts in this biological context. The in vivo rescue effect of a bilberry extract supplemented diet on Drosophila melanogaster was assessed by monitoring biochemical and genomic markers. Hemolymph trehalose levels were halved upon addition of 3% hydro-methanolic bilberry extract to a high-sugar (1.5 M sucrose) diet, as compared to the non-supplemented high-sugar diet. Noteworthy, the rescue seen for flies kept on the bilberry extract supplemented high-sugar diet appeared to parallel the trehalose levels observed in the case of the control diet (50 mM sucrose) flies. Moreover, next to the trehalose-lowering type of in vivo effects, other gene expression related rescues were also detected for genes such as InR, Akh, AstA, AstC, Irk, Npc2g, and CCHa2 upon supplementation of the high-sugar diet with our hydro-methanolic bilberry fruit extract. Our findings suggest that such a bilberry fruit extract could generate physiological and genomic type of compensatory mechanisms so that further translational approaches would advance the understanding of some human specific pathological conditions.

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UJI IMUNOSTIMULATOR DARI KOMBINASI EKSTRAK ETANOL HERBA BINARA (ARTEMISIA VULGARIS LINN) DAN EKSTRAK ETANOL DAUN PUCUK MERAH (SYZYGIUM OLEANA) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT PADA TIKUS JANTAN

Jurnal Penelitian Farmasi & Herbal ◽

10.36656/jpfh.v1i2.105 ◽

2019 ◽

Vol 1 (2) ◽

pp. 22-26

Author(s):

Romauli Anna Teresia Marbun ◽

Aminah Syarifuddin ◽

Montysory Silalahi ◽

Radika Bella Fista Ginting

Keyword(s):

Antioxidant Activity ◽

Immune System ◽

Secondary Metabolites ◽

Ethanol Extract ◽

Positive Control ◽

Artemisia Vulgaris ◽

Chemical Screening ◽

Chemical Content ◽

High Antioxidant Activity ◽

New Treatments

Diseases mediated by the immune system are difficult problems to treat such as human immunodeficiency virus (HIV) and other lethal viruses. Infections that occur in normal people are generally brief and rarely leave permanent damage. Treatment of this disease requires an aggressive and innovative approach to the development of new treatments so that it requires the role of immunomodulators to improve the immune system. A substance that acts as an enhancer or immune enhancer can be obtained by using herbs that are efficacious as immunostimulants. One of the herbs used is herbal binara (Artemisia vulgaris L) which has been studied as a potential immunomodulator with high antioxidant activity. Previous research also stated that red shoots (Syzygium oleana) were studied as potential immunomodulators with high antioxidant activity. Several other species such as Syzygium samarangense have 16 flavonoida compounds which show pharmacological immunological activity. The purpose of this study was to determine the content of secondary metabolites of ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) and to determine the best dose of extract from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) can reduce the volume of swelling of mouse feet. Examination of the chemical content of secondary metabolites from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) is carried out by chemical screening and characterization of simplicia and extract. The method used is the slow type hypersensitivity method. In this test the independent variable is the secondary metabolite of ethanol extract of herb binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) with four concentrations (50, 100, 200 and 400 mg / kgBB). The positive control used by Stimuno dose is 32.5 mg / kgBB

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Composition and Antioxidant Activity of Aqueous Extracts Obtained from Herb of Tansy (Tanacetum Vulgare L.)

Revista de Chimie ◽

10.37358/rc.18.5.6257 ◽

2018 ◽

Vol 69 (5) ◽

pp. 1041-1044

Author(s):

Claudia Anca Mot ◽

Andreea I. Lupitu ◽

Simona Bungau ◽

Ciprian Iovan ◽

Dana Maria Copolovici ◽

...

Keyword(s):

Antioxidant Activity ◽

Ascorbic Acid ◽

Veterinary Medicine ◽

Pharmaceutical Industry ◽

Phenolic Content ◽

Food Preservation ◽

Aqueous Extracts ◽

Tanacetum Vulgare ◽

High Antioxidant Activity ◽

Natural Additives

The aim of this study was to determine the phenolic content and antioxidant activity of extracts obtained from herb of Tanacetum vulgare L. (tansy) harvested in two different locations from the western part of Romania. Aqueous extracts of tansy presented high antioxidant activity that ranged between 295.77 and 653.65 mg GAE/L, and remarkable percentage of inhibition (31.82-65.05 %). The compounds quantified in the plant extracts that were determined by UHPLC are: ascorbic acid, riboflavin, pyrocatechol, rutin, quercetin, and kaempferol. Extracts obtained from tansy flowers showed higher content of phenols compared to that obtained from leaves. Similarly, the flower extracts present a high antioxidant activity that could suggest their use as natural additives in food preservation and also in pharmaceutical industry and veterinary medicine.

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