Chemical Composition, Antimicrobial Activity and Potential Cytotoxic Effect of Mentha Viridis (Spearmint) Extracts from Saudi Arabia

Many medicinal plants have been used to treat and prevent illnesses in Saudi Arabia. The present study aimed to investigate the chemical composition of Mentha viridis obtainedfrom Albaha region of Saudi Arabiaand evaluate its antimicrobial and antiproliferative potential. The extract was obtained from plant fresh material and identified by gas chromatography-mass spectrometry (GC-MS). The antimicrobial and antiproliferative potential of the plant extract was analysed by performing four subsequent extracts: ethanol, petroleum ether, chloroform, and methanol. The GC-MS analysis showed carvone as a main component, as it comprised 64.82 % of the plant extract. In antimicrobial activity, methanol extract showed significant activityagainst Pseudomonas aeruginosa with zone of inhibition of 15 mm. The MTT assay showed thatpetroleum ether and chloroform extracts have moderate cytotoxic effect against MCF-7 breast cancer cell line with IC50 values of 193.23 μg/mL and 131.86 μg/mL, respectively. Chloroform extract also showed mild activity against HCT-116 colorectal cancer cell line with IC50value of 189.2 μg/mL. This study highlights the potential of M. viridis extracts as powerful bioactive phytochemicals with possible role in diseases and cancer therapy.

Download Full-text

Anticarcinogenic effect of Cyclotrichium origanifolium on human colorectal cancer cell line and associating with its antioxidative properties

Planta Medica ◽

10.1055/s-0031-1282647 ◽

2011 ◽

Vol 77 (12) ◽

Author(s):

S Rostami ◽

F Oke Altuntas ◽

B Aslim ◽

H Duman

Keyword(s):

Colorectal Cancer ◽

Cell Line ◽

Cancer Cell ◽

Cancer Cell Line ◽

Colorectal Cancer Cell ◽

Colorectal Cancer Cell Line ◽

Human Colorectal Cancer ◽

Antioxidative Properties ◽

Human Colorectal Cancer Cell

Download Full-text

Synthesis and Anticancer Activity of New Hydrazide-hydrazones and Their Pd(II) Complexes

Letters in Drug Design & Discovery ◽

10.2174/1570180815666180816124102 ◽

2019 ◽

Vol 16 (5) ◽

pp. 522-532 ◽

Cited By ~ 1

Author(s):

Bedia Kocyigit-Kaymakcioglu ◽

Senem Sinem Yazici ◽

Fatih Tok ◽

Miriş Dikmen ◽

Selin Engür ◽

...

Keyword(s):

Cytotoxic Activity ◽

Anticancer Activity ◽

Cell Line ◽

Cancer Cell ◽

Cytotoxic Effect ◽

A549 Cells ◽

Cancer Cell Line ◽

Fibroblast Cell ◽

Reference Drug ◽

A549 Cancer Cell Line

Background: Hydrazones, one of the important classes of organic molecules, are pharmaceutical agents comprising –CO-NH-N=CH- group in the structure therefore and exhibiting significant biological activity. Methods: 5-Chloro-N’-[(substituted)methylidene] pyrazine-2-carbohydrazide (3a-g) and their Pd(II) complexes (4a-h) were synthesized and investigated in vitro anticancer activity on A549, Caco2 cancer and normal 3T3 fibroblast cell lines, using the MTT assay. Results: Anticancer activity screening results revealed that some compounds showed remarkable cytotoxic effect. Among them, 5-chloro-N'-[(4-hydroxyphenyl)methylidene] pyrazine-2-carbohydrazide (3c) displayed higher cytotoxic activity against A549 cancer cell line than the reference drug cisplatin. Conclusion: Compound 3c showed high cytotoxic activity against A549 cancer cell line but it showed low cytotoxic effect against normal 3T3 fibroblast cell line. Antiproliferative and antimetastatic effects of 3c were determined by the real-time monitoring of cell proliferative system (RTCA DP). The cell proliferation, metastatic and invasive activities of A549 cells were decreased due to increased concentration of 3c.

Download Full-text

Synthesis, Cytotoxicity and Antioxidant Activity of New Analogs of RC-121 Synthetic Derivatives of Somatostatin

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520618666180417164344 ◽

2019 ◽

Vol 18 (10) ◽

pp. 1417-1424 ◽

Cited By ~ 2

Author(s):

Emilia Naydenova ◽

Diana Wesselinova ◽

Svetlana Staykova ◽

Ivan Goshev ◽

Ljubomir Vezenkov

Keyword(s):

Antioxidant Activity ◽

Antioxidant Capacity ◽

Cell Line ◽

Cancer Cell ◽

Cancer Cell Line ◽

Colorectal Cancer Cell Line ◽

Cervical Cancer Cell Line ◽

Synthetic Derivatives ◽

Derivatives Of

Background: Based on the structure of RC-121 (D-Phe-c (Cys-Tyr-D-Trp-Lys-Val-Cys)-Thr-NH2, - synthetic derivatives of somatostatin), some analogs were synthesized and tested for in vitro cytotoxic and antioxidant activity. Objectives: The new analogs were modifyed at position 5 with Dap (diaminopropanoic acid), Dab (diaminobutanoic acid) and Orn and at position 6 with the unnatural amino acids Tle (t-leucine). Methods: The in vitro cytotoxic effects of the substances were investigated against a panel of human tumor cell lines HT-29 (Human Colorectal Cancer Cell Line), MDA-MB-23 (Human Breast Cancer Cell Line), Hep G-2 (Human Hepatocellular Carcinoma Cell Line) and HeLa (cervical cancer cell line). The antioxidant capacities were tested by ORAC (Oxygen Radical Antioxidant Capacity) and HORAC (Hydroxyl Radical Averting Capacity) methods. Results: All substances expressed significantly higher antioxidant capacity by comparison with galic acid and Trolox. All substances showed considerable antioxidant capacity as well. Compound 2T (D-Phe-c(Cys-Tyr-DTrp- Dap-Tle-Cys)-Thr-NH2)had the highest antioxidant effect. The compound 4T (D-Phe-c(Cys-Tyr-D-Trp- Orn-Tle-Cys)-Thr-NH2) displayed antiproliferative effect on HeLa cells with IC50 30 µM. The peptide analog 3T (D-Phe-c(Cys-Tyr-D-Trp-Lys-Tle-Cys)-Thr-NH2) exerted the most pronounced inhibition on the cell vitality up to 53%, 56% and 65% resp. against MDA-MB-23, Hep G-2, HeLa in the higher tested concentration. Conclusion: The somatostatin analogs showed moderate influence on the vitality of different tumor cells and could be used in changing their pathology.

Download Full-text