scholarly journals Shewanella baltica strain JD0705 isolated from the mangrove wetland soils in Thailand and characterization of its ligninolytic performance

2020 ◽  
Vol 22 (1) ◽  
Author(s):  
Aiya Chantarasiri

Abstract. Chantarasiri A. 2021. Shewanella baltica strain JD0705 isolated from the mangrove wetland soils in Thailand and characterization of its ligninolytic performance. Biodiversitas 22: 354-361. Lignin is a complex biopolymer and the third component by mass of lignocellulosic plant biomass. Its recalcitrant property hampers the hydrolysis and utilization of cellulose and hemicellulose in the lignocellulosic biomass. Thus, the delignification of lignocellulosic biomass is an enormous challenge for emerging bio-based applications. This study presented a potential ligninolytic bacterium for potential use in the biological delignification process under mild conditions. This bacterium was isolated from the mangrove wetland soils in Thailand, characterized and identified as psychrotrophic Shewanella baltica strain JD0705. It was determined for ligninolytic activity and showed laccase activity at 5.23 ± 0.10 U/mL. The optimum temperature and pH for the laccase activity were observed to be 25°C at a pH of 7.0 respectively with a stability range of 20-30°C temperature and pH of 7.0-8.0. S. baltica strain JD0705 was used in the biological delignification of rice husk powder and promoted the hydrolysis of rice husk powder to obtain more liberating sugar content. The findings from this study indicated that feasibility of using this ligninolytic bacterium for the production of laccase and the delignification of lignocellulosic plant biomass.

2013 ◽  
Vol 37 ◽  
pp. 108-118 ◽  
Author(s):  
Jian He ◽  
Yuxin Jie ◽  
Jianhong Zhang ◽  
Yuzhen Yu ◽  
Guoping Zhang

2021 ◽  
Vol 22 (11) ◽  
pp. 5781
Author(s):  
Janarthanan Supramaniam ◽  
Darren Yi Sern Low ◽  
See Kiat Wong ◽  
Loh Teng Hern Tan ◽  
Bey Fen Leo ◽  
...  

Cellulose nanofibers (CNF) isolated from plant biomass have attracted considerable interests in polymer engineering. The limitations associated with CNF-based nanocomposites are often linked to the time-consuming preparation methods and lack of desired surface functionalities. Herein, we demonstrate the feasibility of preparing a multifunctional CNF-zinc oxide (CNF-ZnO) nanocomposite with dual antibacterial and reinforcing properties via a facile and efficient ultrasound route. We characterized and examined the antibacterial and mechanical reinforcement performances of our ultrasonically induced nanocomposite. Based on our electron microscopy analyses, the ZnO deposited onto the nanofibrous network had a flake-like morphology with particle sizes ranging between 21 to 34 nm. pH levels between 8–10 led to the formation of ultrafine ZnO particles with a uniform size distribution. The resultant CNF-ZnO composite showed improved thermal stability compared to pure CNF. The composite showed potent inhibitory activities against Gram-positive (methicillin-resistant Staphylococcus aureus (MRSA)) and Gram-negative Salmonella typhi (S. typhi) bacteria. A CNF-ZnO-reinforced natural rubber (NR/CNF-ZnO) composite film, which was produced via latex mixing and casting methods, exhibited up to 42% improvement in tensile strength compared with the neat NR. The findings of this study suggest that ultrasonically-synthesized palm CNF-ZnO nanocomposites could find potential applications in the biomedical field and in the development of high strength rubber composites.


Molecules ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 74
Author(s):  
Abhishek RoyChowdhury ◽  
Pallabi Mukherjee ◽  
Saumik Panja ◽  
Rupali Datta ◽  
Christos Christodoulatos ◽  
...  

The use of insensitive munitions such as 3-nitro-1,2,4-triazol-5-one (NTO) is rapidly increasing and is expected to replace conventional munitions in the near future. Various NTO treatment technologies are being developed for the treatment of wastewater from industrial munition facilities. This is the first study to explore the potential phytoremediation of industrial NTO-wastewater using vetiver grass (Chrysopogon zizanioides L.). Here, we present evidence that vetiver can effectively remove NTO from wastewater, and also translocated NTO from root to shoot. NTO was phytotoxic and resulted in a loss of plant biomass and chlorophyll. The metabolomic analysis showed significant differences between treated and control samples, with the upregulation of specific pathways such as glycerophosphate metabolism and amino acid metabolism, providing a glimpse into the stress alleviation strategy of vetiver. One of the mechanisms of NTO stress reduction was the excretion of solid crystals. Scanning electron microscopy (SEM), electrospray ionization mass spectrometry (ESI-MS), and Fourier-transform infrared spectroscopy (FTIR) analysis confirmed the presence of NTO crystals in the plant exudates. Further characterization of the exudates is in progress to ascertain the purity of these crystals, and if vetiver could be used for phytomining NTO from industrial wastewater.


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