Study of the in vitro Antibacterial Activity of the Aqueous and Hydroethanolic Extracts from the Leaves of the Erythrococca anomala (Euphorbiaceae) on Six Bacterial Strains

Synthesis of 1,2,3-triazole-substituted coumarins and also 1,2,3-triazolyl or 1,2,3-triazolylalk-1-inyl-linked coumarin-2,3-furocoumarin hybrids was performed by employing the cross-coupling and copper catalyzed azide-alkyne cycloaddition reaction approaches. The synthesized compounds were evaluated for their in vitro antibacterial activity against Staphylococcus aureus, Bacillius subtilis, Actinomyces viscosus and Escherichia coli bacterial strains. Coumarin-benzoic acid hybrids 4с, 42с and 3-((4-acetylamino-3-(methoxycarbonyl)phenyl)ethynyl)coumarin (29) showed promising activity against S. aureus strains, and the 1,2,3-triazolyloct-1-inyl linked coumarin-2,3-furocoumarin hybrid 37c was endowed with high selectivity against B. subtilis and E. coli species. The in vitro antibacterial activity of 4с, 29, 37c and 42с can potentially be compared with that of a number of modern antibiotic drugs used in the clinic, suggesting promising prospects for further research. A detailed study of the molecular interactions with the targeted protein MurB was performed using docking simulations and the obtained results are quite promising.

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Design, Synthesis and Antibacterial Evaluation of 3-Substituted Ocotillol-Type Derivatives

Molecules ◽

10.3390/molecules23123320 ◽

2018 ◽

Vol 23 (12) ◽

pp. 3320 ◽

Cited By ~ 1

Author(s):

Kai-Yi Wang ◽

Zhi-Wen Zhou ◽

Heng-Yuan Zhang ◽

Yu-Cheng Cao ◽

Jin-Yi Xu ◽

...

Keyword(s):

Antibacterial Activity ◽

Triterpenoid Saponin ◽

Bacterial Strains ◽

Design Synthesis ◽

Design And Synthesis ◽

Good Antibacterial Activity ◽

Structure Activity ◽

In Vitro Antibacterial Activity ◽

Free Hydroxyl

Antibiotic resistance has become a serious global problem that threatens public health. In our previous work, we found that ocotillol-type triterpenoid saponin showed good antibacterial activity. Based on preliminary structure-activity relationship, novel serious C-3 substituted ocotillol-type derivatives 7–26 were designed and synthesized. The in vitro antibacterial activity was tested on five bacterial strains (B. subtilis 168, S. aureus RN4220, E. coli DH5α, A. baum ATCC19606 and MRSA USA300) and compared with the tests on contrast. Among these derivatives, C-3 position free hydroxyl substituted compounds 7–14, showed good antibacterial activity against Gram-positive bacteria. Furthermore, compound 22 exhibited excellent antibacterial activity with minimum inhibitory concentrations (MIC) values of 2 μg/mL against MRSA USA300 and 4 μg/mL against B. subtilis. The structure-activity relationships of all current ocotillol-type derivatives our team synthesised were summarized. In addition, the prediction of absorption, distribution, metabolism, and excretion (ADME) properties and the study of pharmacophores were also conducted. These results can provide a guide to further design and synthesis works.

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Chemoinformatics Approach to Antibacterial Studies of Essential Oils

Natural Product Communications ◽

10.1177/1934578x1501000667 ◽

2015 ◽

Vol 10 (6) ◽

pp. 1934578X1501000 ◽

Cited By ~ 4

Author(s):

Dragoljub L. Miladinović ◽

Budimir S. Ilić ◽

Branislava D. Kocić

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Antibacterial Activities ◽

Bacterial Strains ◽

Antibacterial Studies ◽

Action Mechanisms ◽

In Vitro Antibacterial Activity ◽

Checkerboard Assay ◽

Antagonistic Interactions

The chemical composition and antibacterial activity of Nepeta nuda (Lamiaceae) essential oil were examined, as well as the association between it and standard antibiotics: tetracycline and streptomycin. The antibacterial activities of 1,8-cineole, the main constituent of N. nuda oil, individually and in combination with standard antibiotics were also determined. The interactions of the essential oil and 1,8-cineole with antibiotics toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemoinformatics methods. Oxygenated monoterpenes were the most abundant compound class in the oil (57.8%), with 1,8-cineole (46.0%) as the major compound. The essential oil exhibited in vitro antibacterial activity against all tested bacterial strains, but the activities were lower than those of the standard antibiotics. The combinations N. nuda oil-antibiotic and 1,8-cineole-antibiotic produced a predominantly antagonistic interactions. Chemoinformatics survey confirms the antagonistic interactions as a consequence of membrane potential/proton motive force dissipation. These data indicate cytochrome c oxidase as a target for 1.8-cineole toxicity action mechanisms.

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In vitro antibacterial activity of Camellia sinensis leaf extracts to some selective pathogenic bacterial strains

International Journal of Biosciences (IJB) ◽

10.12692/ijb/3.9.69-75 ◽

2013 ◽

Vol 3 (9) ◽

pp. 69-75 ◽

Cited By ~ 2

Keyword(s):

Antibacterial Activity ◽

Camellia Sinensis ◽

Bacterial Strains ◽

Leaf Extracts ◽

In Vitro Antibacterial Activity

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Evaluation of Antibacterial Activity of Acer syriacum by Bioautography Method

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2016.9.2.9 ◽

1970 ◽

Vol 9 (2) ◽

pp. 3215-3219

Author(s):

B. Al Asfour ◽

R. Al-kayali ◽

A. Kitaz

Keyword(s):

Antibacterial Activity ◽

Methanolic Extract ◽

Ethyl Acetate Extract ◽

Diffusion Method ◽

Klebsiella Pneumonia ◽

Bacterial Strains ◽

In Vitro Antibacterial Activity ◽

Inhibition Zones ◽

Layer Chromatography

antibacterial activity of leaves, fruits and bark of acer syriacum was evaluated against both negative and positive gram bacterial strains. The powder of above parts were sequentially extracted with aqueous methanol 80%, acetone and petroleumether by maceration. The in vitro antibacterial activity of A. syriacum extracts (in concentration of 200 mg/mL) was performed by agar well diffusion method. Among the tested extracts, leaves methanolic extract has shown the highest growth inhibition. Further, the extract was fractionated, separated by thin layer chromatography (TLC) and submitted to bioautography. the results indicate that the ethyl acetate extract had the most efficient antibacterial compounds that displayed inhibition zones (25 mm) against Staphylococcus aureus ATCC25923 and (28mm) against Klebsiella pneumonia ATCC700603. the clear zones were located at RF= 0.15.

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Chemical Composition and Antibacterial Activity of the Essential Oil of Retrohpyllum rospigliosii Fruits from Colombia

Natural Product Communications ◽

10.1177/1934578x1000500732 ◽

2010 ◽

Vol 5 (7) ◽

pp. 1934578X1000500

Author(s):

Clara E. Quijano-Celis ◽

Mauricio Gaviria ◽

Consuelo Vanegas-López ◽

Ina Ontiveros ◽

Leonardo Echeverri ◽

...

Keyword(s):

Antibacterial Activity ◽

Essential Oil ◽

Diffusion Method ◽

Disc Diffusion ◽

Bacterial Strains ◽

Positive Bacterium ◽

Disc Diffusion Method ◽

In Vitro Antibacterial Activity ◽

Bacterium Bacillus

The essential oil from fruits of Retrophyllum rospigliosii (Pilger) C.N. Page grown in Colombia was analyzed by GC and GC/MS. Ninety-one compounds were identified, of which the most prominent were limonene (37.7%) and α-pinene (16.3%). The in vitro antibacterial activity of the essential oil was studied against seven bacterial strains using the disc diffusion method. The strongest activity of the oil was against the Gram-positive bacterium Bacillus cereus.

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In vitro antibacterial activity of essential oils from Lamiaceae species

The Journal of Phytopharmacology ◽

10.31254/phyto.2016.5404 ◽

2016 ◽

Vol 5 (4) ◽

pp. 141-144

Author(s):

Vinod Kumar ◽

◽

C. S. Mathela ◽

Amit Panwar ◽

◽

...

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Essential Oils ◽

Broad Spectrum ◽

Antibacterial Effect ◽

Gram Negative Bacteria ◽

Bacterial Strains ◽

Gram Negative ◽

In Vitro Antibacterial Activity

Essential oils from Calamintha umbrosa and Nepeta species viz. N. leucophylla; N. hindostana; N. ciliaris and N. clarkei (family Lamiaceae), was tested against six bacterial strains. To evaluate the correlation between the antimicrobial activity and the essential oils, PCA and HCA analysis was done. PCA and HCA analysis of the antibacterial activity revealed that essential oils of Nepeta species had a strong and broad spectrum antibacterial effect against bacterial strains of P. aeruginosa and S. scandidus. The N. leucophylla oil showed higher activity against Gram-negative bacteria P. aeruginosa (10.5 mm, MIC 10 µL/mL) and K. pneumonia (9.1 mm, MIC 45 µL/mL) among all Nepeta oils which may be due to presence of active antimicrobial iridoids compounds.

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