Comprehensive analysis of small RNAs expressed in developing male strobili of Cryptomeria japonica

Abstract Clonal differences in fertility (expressed as the number of female and male strobili) were determined for three consecutive years (2002-2004) in a clonal seed orchard of sugi (Cryptomeria japonica D. Don) in Korea. Fertility varied among clones and among years producing three-year averages of 196 and 652 for female and male strobili per ramet, respectively. Correlation between female and male strobilus production was positive over the three years and statistically significant in 2003, a good flowering year. Based on the observed fertility variation, the status numbers (Ns, measure of genetic diversity) were calculated and varied from 25.6 to 31.7 among the three studied years. On average (pooled), relative status number was 86% of the census number (N). Variation in female fertility was higher than that in male fertility, and this variation was reflected on female and male parents’ status numbers. Pooled Ns estimated from the three years was higher than that for any single year, implying that genetic diversity would increase when seeds collected from different years are pooled.

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"For Countermeasures against Sugi Pollinosis in Forest Science", Formation of Cryptomeria japonica Male Strobili and Its Suppression.

Journal of the Japanese Forest Society ◽

10.4005/jjfs.92.304 ◽

2010 ◽

Vol 92 (6) ◽

pp. 304-309

Author(s):

Kenji Shinohara

Keyword(s):

Cryptomeria Japonica ◽

Forest Science ◽

Male Strobili

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Inspecting abundantly expressed genes in male strobili in sugi (Cryptomeria japonica D. Don) via a highly accurate cDNA assembly

10.1101/2020.04.21.054320 ◽

2020 ◽

Cited By ~ 2

Author(s):

Fu-Jin Wei ◽

Saneyoshi Ueno ◽

Tokuko Ujino-Ihara ◽

Maki Saito ◽

Yoshihiko Tsumura ◽

...

Keyword(s):

Transcription Factors ◽

Functional Genomics ◽

Cryptomeria Japonica ◽

Evolutionary Biology ◽

De Novo ◽

Transcriptome Assembly ◽

Principal Component ◽

Short Period ◽

Three Stages ◽

Male Strobili

AbstractSugi (Cryptomeria japonica D. Don) is an important conifer used for afforestation in Japan. The field of functional genomics is rapidly developing. The genomics of this gymnosperm species is currently being studied. Although its genomic size is 11 Gbps, it is still too large to assemble well within a short period of time. Transcriptomics is the one another approach to address this. Moreover, it is a necessary step in obtaining the complete genomic data. Here we designed a three stages assembling workflow using the de novo transcriptome assembly tools, Oases and Trinity. The three stages in transcriptomics are independent assembly, automatic and semi-automatic integration, and refinement by filtering out potential contamination. We found a set of 49,795 cDNA and an equal number of translated proteins (CJ3006NRE). According to the benchmark of BUSCO, 87.01 % were complete genes, including very high “Complete and single-copy” genes–78.47%. Compared to other full-length cDNA resources, the extent of the coverage in CJ3006NRE suggests that it may be used as the standard for further studies. When two tissue-specific libraries were compared, principal component analysis (PCA) showed that there were significant differences between male strobili and leaf and bark sets. The highest three upregulated transcription factors stood out as orthologs to angiosperms. The identified signature-like domain of the transcription factors demonstrated the accuracy of the assembly. Based on the evaluation of different resources, we demonstrate that our transcriptome assembly output is valuable and useful for further studies in functional genomics and evolutionary biology.

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Characterization of expressed sequence tags from a full-length enriched cDNA library of Cryptomeria japonica male strobili

BMC Genomics ◽

10.1186/1471-2164-9-383 ◽

2008 ◽

Vol 9 (1) ◽

pp. 383 ◽

Cited By ~ 59

Author(s):

Norihiro Futamura ◽

Yasushi Totoki ◽

Atsushi Toyoda ◽

Tomohiro Igasaki ◽

Tokihiko Nanjo ◽

...

Keyword(s):

Cdna Library ◽

Expressed Sequence Tags ◽

Cryptomeria Japonica ◽

Full Length ◽

Expressed Sequence ◽

Male Strobili

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Factors Affecting the Number of Pollen Grains per Male Strobilus in Japanese Cedar (Cryptomeria japonica)

Plants ◽

10.3390/plants10050856 ◽

2021 ◽

Vol 10 (5) ◽

pp. 856

Author(s):

Hiroyuki Kakui ◽

Eriko Tsurisaki ◽

Rei Shibata ◽

Yoshinari Moriguchi

Keyword(s):

Genetic Variation ◽

Statistical Modeling ◽

Cryptomeria Japonica ◽

Pollen Grains ◽

Japanese Cedar ◽

Equation Modeling ◽

Factors Affecting ◽

Pollen Number ◽

Male Strobilus ◽

Male Strobili

Japanese cedar (Cryptomeria japonica) is the most important timber species in Japan; however, its pollen is the primary cause of pollinosis in Japan. The total number of pollen grains produced by a single tree is determined by the number of male strobili (male flowers) and the number of pollen grains per male strobilus. While the number of male strobili is a visible and well-investigated trait, little is known about the number of pollen grains per male strobilus. We hypothesized that genetic and environmental factors affect the pollen number per male strobilus and explored the factors that affect pollen production and genetic variation among clones. We counted pollen numbers of 523 male strobili from 26 clones using a cell counter method that we recently developed. Piecewise Structural Equation Modeling (pSEM) revealed that the pollen number is mostly affected by genetic variation, male strobilus weight, and pollen size. Although we collected samples from locations with different environmental conditions, statistical modeling succeeded in predicting pollen numbers for different clones sampled from branches facing different directions. Comparison of predicted pollen numbers revealed that they varied >3-fold among the 26 clones. The determination of the factors affecting pollen number and a precise evaluation of genetic variation will contribute to breeding strategies to counter pollinosis. Furthermore, the combination of our efficient counting method and statistical modeling will provide a powerful tool not only for Japanese cedar but also for other plant species.

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New Source Book Offers Comprehensive Analysis of Kinship Care Policies and Practices

PsycEXTRA Dataset ◽

10.1037/e724542011-001 ◽

2003 ◽

Keyword(s):

Kinship Care ◽

Comprehensive Analysis ◽

Source Book ◽

Care Policies ◽

Policies And Practices

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Sozial-räumliche Dynamiken der Agrartreibstoffe.

PROKLA Zeitschrift für kritische Sozialwissenschaft ◽

10.32387/prokla.v46i184.123 ◽

2016 ◽

Vol 46 (184) ◽

pp. 423-440 ◽

Cited By ~ 1

Author(s):

Kristina Dietz ◽

Bettina Engels ◽

Oliver Pye

Keyword(s):

Political Economy ◽

Capital Flows ◽

Spatial Dynamics ◽

Financial Sector ◽

Comprehensive Analysis ◽

Spatial Theory ◽

Global Political Economy

This article explores the spatial dynamics of agrofuels. Building on categories from the field of critical spatial theory, it shows how these categories enable a comprehensive analysis of the spatial dynamics of agrofuels that links the macro-structures of the global political economy to concrete, place-based struggles. Four core socio-spatial dynamics of agrofuel politics are highlighted and applied to empirical findings: territorialization, the financial sector as a new scale of regulation, place-based struggles and transnational spaces of resources and capital flows.

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Concerning the abuse of power by an official

Scientific and Informational Bulletin of Ivano-Frankivsk University of Law Named after King Danylo Halytskyi - Scientific and informational bulletin of Ivano-Frankivsk University of Law named after King Danylo Halytskyi ◽

10.33098/2078-6670.2020.9.21.103-110 ◽

2020 ◽

pp. 103-110

Author(s):

Ihor Oheruk

Keyword(s):

Criminal Code ◽

Comprehensive Analysis ◽

Practical Significance ◽

Abuse Of Power ◽

Government Functions ◽

Analysis And Synthesis ◽

The Status ◽

The Subject ◽

Criminal Legislation ◽

Logical Semantic

Purpose. The purpose of the work is to analyze the application of the second and third parts of Article 3692 of the Criminal Code of Ukraine to officials in the context, that defines them by the Criminal Code of Ukraine in the note to Article 364 of the Criminal Code of Ukraine. Methodology. The methodology includes a comprehensive analysis and synthesis of the available scientific and theoretical material and the formulation of relevant conclusions and recommendations. In the course of the study, the following methods of scientific knowledge were used: terminological, logical-semantic, system-structural, logical-normative. Results: in the course of research the cause of criminalization of such act as "abuse of power" is considered, the subject of the specified criminal act which has the features of "an official" in the context, that defines it by the note to Article 364 of the Criminal Code of Ukraine is analyzed and the main ways of committing criminal acts, that are provided for in this article of the Criminal Code of Ukraine are identified. Originality. The study found, that one of the key conditions for the opportunity to influence officials, that are authorized to perform government or local self-government functions, is the position held by the official and the related opportunities. Therefore, taking into account the opinion of the scientists, that the subject of crimes, that are provided for by the second and third parts of Article 3692 is special, the peculiarities of which is the cumulative feature, that denotes, that such person is not endowed with the status of an official, well-founded need to specify the criminal legislation of Ukraine in terms of the application the second and third parts of Article 3692 of the Criminal code of Ukraine concerning officials in the context, that defines them by the criminal legislation of Ukraine in the note to Article 364 of the Criminal Code of Ukraine. Practical significance. The research results can be used in lawmaking in the improvement of anti-corruption legislation.

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