Salivary levels of Streptococcus mutans and Lactobacilli and other salivary indices in patients wearing clear aligners versus fixed orthodontic appliances: An observational study

Background: Fixed orthodontic appliances, such as Edgewise and Straightwire techniques, can increase the amount of plaque retention containing Streptococcus mutans (S. mutans), which can lead to white spot lesions. Purpose: The aim of this study is to analyse the correlation of fixed orthodontic treatment with Edgewise and Straightwire techniques on the incidence of white spot lesions and accumulation of S. mutans. Methods: The samples consisted of three groups: control group (n=8), Edgewise technique group, and Straightwire technique group. We observed the samples at the sixth month and eighth month of the treatment, after the installation of the fixed orthodontic appliances. The observation of white spot lesions with caries detector was applied in all regions. Bacterial swabs were acquired in the lateral incisor region, then a bacterial culture procedure was carried out on selective media of S. mutans, and then a bacterial count was performed. The data was analysed using two-way ANOVA, the post-hoc least square differences test, and the Pearson’s correlation test. Results: The number of white spot lesions in the Edgewise group was higher than in the Straightwire group in the sixth and eighth month of treatment with insignificant difference (p>0.05). The number of S. mutans bacteria increased in all groups, but there were no significant differences (p>0.05). There was no significant relationship between the number of white spot lesions with the accumulation of S. mutans between groups (p>0.05). Conclusion: The Edgewise and Straightwire techniques increase the incidence of white spot lesions but accumulation of S. mutans with the incidence of white spot lesions has no relationship.

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Microbiological Evaluation of 0.2% Chlorhexidine Gluconate Mouth Rinse in Orthodontic Patients

The Angle Orthodontist ◽

10.2319/080506-320.1 ◽

2007 ◽

Vol 77 (5) ◽

pp. 881-884 ◽

Cited By ~ 20

Author(s):

Emel Sari ◽

Ilhan Birinci

Keyword(s):

Streptococcus Mutans ◽

Orthodontic Treatment ◽

Chlorhexidine Gluconate ◽

Wilcoxon Test ◽

Orthodontic Appliances ◽

Mouth Rinse ◽

Fixed Orthodontic Appliances ◽

Orthodontic Patients ◽

Fixed Appliances

Abstract Objective: To assess the effectiveness of 0.2% chlorhexidine gluconate mouth rinse on Streptococcus mutans and lactobacilli in orthodontic patients with fixed appliances. Materials and Methods: Twenty patients, aged 13–18, with fixed orthodontic appliances participated in the study. The levels of S mutans and lactobacilli in saliva samples were evaluated at four stages: at the beginning of the orthodontic treatment, at least 2 weeks after the bonding of brackets, 1 week after the introduction of 0.2% chlorhexidine gluconate mouth rinse, and at the fourth week. The changes in S mutans and lactobacilli levels were analyzed via Wilcoxon test. Results: Increases in bacterial levels of S mutans and lactobacilli were detected after the orthodontic appliances were bonded. A significant decrease in S mutans levels was observed 1 week after the introduction of chlorhexidine mouth rinse. Conclusions: An 0.2% chlorhexidine gluconate mouth rinse decreased S mutans levels, but had no effect on lactobacilli levels.

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Effects of the fixed orthodontic therapy on biochemical and microbiological parameters of saliva

Srpski arhiv za celokupno lekarstvo ◽

10.2298/sarh190806016i ◽

2020 ◽

Vol 148 (5-6) ◽

pp. 270-274

Author(s):

Tanja Ivanovic ◽

Ljiljana Stojanovic ◽

Dragan Ivanovic ◽

Predrag Nikolic ◽

Zeljko Milosavljevic ◽

...

Keyword(s):

Streptococcus Mutans ◽

Ph Value ◽

Periodontal Diseases ◽

Buffering Capacity ◽

Control Group ◽

Orthodontic Appliances ◽

Orthodontic Therapy ◽

Fixed Orthodontic Appliances ◽

Number Of Bacteria ◽

Lactobacillus Spp

Introduction/Objective. Malocclusions are one of the most frequent disorders in dentistry, and pose a risk for the onset of caries and periodontal diseases. Fixed orthodontic treatment solves the problem of malocclusions; however, it requires simultaneous cooperation of the patients, parents, and dentists involved. The objective of this study is to examine the effects of fixed orthodontic therapy on the Streptococcus mutans and Lactobacillus spp. bacteria in saliva, the pH value, and buffering capacity of saliva. Methods. The research was carried out at the Faculty of Medicine in Foca, Department of Dentistry. The study included 100 respondents, aged 13 to 17 years. The respondents were divided into two groups: the study group (respondents wearing fixed braces) and the control group (respondents not subjected to fixed braces therapy). Saliva samples were taken from the respondents four, 12, and 18 weeks after the start of the orthodontic therapy. The study used the bacteria caries risk test (CRT) and CRT buffer (Ivoclar Vivadent). Results. The study showed an increased number of bacteria in saliva of the respondents during all three follow-up periods (?2 test, p = 0.001). The largest numbers of the Streptococcus mutans and Lactobacillus spp. bacteria were found in week 12 of the therapy. Saliva pH value and buffering capacity of saliva increased statistically significantly in week 12 of the therapy (?2 test, p = 0.001). Conclusion. Oral conditions in patients changed during the fixed orthodontic therapy: the number of bacteria increased, the pH value and buffering capacity of saliva changed. It was necessary to use preventive measures in order to avoid complications during the fixed orthodontic appliances therapy.

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The Effect of Gargling with Aqueous Extract of Dandelion (Taraxacum officinale) on the Oral Hygiene Status of Patients Wearing Fixed Orthodontic Appliance: A Clinical Study

Iraqi Dental Journal ◽

10.46466/idj.v40i1.135 ◽

2018 ◽

Vol 40 (1) ◽

pp. 1-4

Author(s):

Munad Jihad Al duliamy

Keyword(s):

Streptococcus Mutans ◽

Aqueous Extract ◽

Oral Hygiene ◽

Orthodontic Treatment ◽

Taraxacum Officinale ◽

Orthodontic Appliances ◽

Tooth Cleaning ◽

Fixed Orthodontic Appliances ◽

Wide Range ◽

Fixed Appliances

Background: During the course of fixed orthodontic therapy, it is difficult to maintain high standard oral hygiene care by mechanical tooth cleaning only. Therefore, mouthwashes are commonly used as adjunct for mechanical tooth cleaning. Most of the mouthwashes are chemicals and have a wide range of side effects. Plant extracts have stood the test of time as safe inexpensive and available remedy for providing general health care. Objective: The present study attempted to evaluate the efficacy of gargling with aqueous extract of Dandelion (Taraxacum officinale) on the oral hygiene status of patients wearing fixed orthodontic appliances. Methods: Eleven male patients undergoing orthodontic treatment with fixed appliances were recruited in the study. All patients were given the same oral hygiene regimen during the study period. Clinical evaluation of OPI (orthodontic plaque index) and salivary streptococcus mutans count were recorded at the first activation visit (three weeks after placement of the orthodontic appliances) which considered the baseline visit and after one week of gargling with the extract. The data were statistically analyzed with SPSS (version 21) using Paired Samples t-test. Result: The results of the statistical analysis revealed that, there were highly significant reductions in both OPI and salivary streptococcus mutans count after one week gargling with aqueous extract of Dandelion (Taraxacum officinale). Conclusion: The present finding concluded that, aqueous extract of Dandelion (Taraxacum officinale) could be use as a promising ingredient in manufacturing mouthwashs and other dental supplies that contribute in maintaining good oral hygiene during the course of orthodontic treatment with fixed appliances.

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Oral colonization of Candida albicans and Streptococcus mutans in children with or without fixed orthodontic appliances: A pilot study

Journal of Dental Sciences ◽

10.1016/j.jds.2021.07.026 ◽

2021 ◽

Author(s):

Fang Yang ◽

Márcia Dinis ◽

Farnoosh Haghighi ◽

Xuesong He ◽

Wenyuan Shi ◽

...

Keyword(s):

Candida Albicans ◽

Pilot Study ◽

Streptococcus Mutans ◽

Orthodontic Appliances ◽

Fixed Orthodontic Appliances ◽

Oral Colonization

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Salivary concentrations of Streptococcus mutans and Lactobacilli during an orthodontic treatment. An observational study comparing fixed and removable orthodontic appliances

Clinical and Experimental Dental Research ◽

10.1002/cre2.261 ◽

2020 ◽

Vol 6 (2) ◽

pp. 181-187 ◽

Cited By ~ 6

Author(s):

Stefano Mummolo ◽

Marco Tieri ◽

Alessandro Nota ◽

Silvia Caruso ◽

Atanaz Darvizeh ◽

...

Keyword(s):

Observational Study ◽

Streptococcus Mutans ◽

Orthodontic Treatment ◽

Orthodontic Appliances ◽

Removable Orthodontic Appliances

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Observational Study Regarding Possible Side Effects of Miniscrew-Assisted Rapid Palatal Expander (MARPE) with or without the Use of Corticopuncture Therapy

Biology ◽

10.3390/biology10030187 ◽

2021 ◽

Vol 10 (3) ◽

pp. 187

Author(s):

Eugen Silviu Bud ◽

Cristina Ioana Bică ◽

Mariana Păcurar ◽

Petru Vaida ◽

Alexandru Vlasa ◽

...

Keyword(s):

Observational Study ◽

Orthodontic Appliances ◽

Maxillary Expansion ◽

Palatal Expansion ◽

Rapid Palatal Expansion ◽

Basal Bone ◽

Fixed Orthodontic Appliances ◽

Before And After ◽

Palatal Mucosa ◽

Mid Palatal Suture

The use of maxillary expanders has the effect of distancing the maxillary bones at the level of the median palatal suture. During maxillary expansion, the main resistance forces occur at the zygomatico-maxillary sutures, and not in the median palatal suture, which is the basic principle on which this method is based. In this observational study, we evaluated possible complications at the skeletal and dentoalveolar level after palatal split using miniscrew-assisted rapid palatal expansion (MARPE) associated or not with corticopuncture (CP) therapy. The study included 27 patients with maxillary transverse deficiency and unilateral or bilateral cross-bite. Skeletal and dentoalveolar changes were evaluated using cone beam computed tomography (CBCT) images acquired before and after expansion. The mid-palatal suture was separated in 88.88% of cases, buccal bone height of the alveolar crest had decreased at first molar both at oral and palatal level by approximately 2.07 mm in 40.7% of cases whilst the remaining 59.3% showed insignificant bone loss, with canines exhibiting buccal tipping of 4.10° in 62.5% of cases. Changes of the occlusal planes were observed in 10 cases (37%). Maxillary canines tended to show symmetric buccal inclinations relative to the maxillary basal bone. Six patients; 22.22% showed hypertrophy/hyperplasia of the palatal mucosa associated with ulcerations, erythema, itching, and discomfort in the area. Swelling at the mid-palatal suture after split was observed in all cases and was caused by the resultant force. No cases of necrosis of the palatal mucosa were observed. Although occlusal modifications occur after palatal split, especially in unilateral cross-bite cases, these changes can be treated with the help of fixed orthodontic appliances.

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The impact of fixed orthodontic appliances on oral microbiome dynamics in Japanese patients

Scientific Reports ◽

10.1038/s41598-020-78971-2 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Isamu Kado ◽

Junzo Hisatsune ◽

Keiko Tsuruda ◽

Kotaro Tanimoto ◽

Motoyuki Sugai

Keyword(s):

Relative Abundance ◽

Japanese Patients ◽

Oral Microbiome ◽

Orthodontic Appliances ◽

Periodontal Pathogens ◽

Supragingival Plaque ◽

Fixed Orthodontic Appliances ◽

Microbial Dna ◽

The Impact ◽

Level Analysis

AbstractFixed orthodontic appliances are common and effective tools to treat malocclusion. Adverse effects of these appliances, such as dental caries and periodontitis, may be associated with alteration of the microbiome. This study investigated the impact of these appliances on the dynamics of the oral microbiome. Seventy-one patients were selected. Supragingival plaque samples were collected before placement (T0) and six months after placement (T1). Saliva samples were collected at T0 and T1, and then when appliance removal (T2). Microbial DNA was analyzed by 16S rRNA meta-sequencing. The diversity analysis indicated dynamic changes in the structure of the oral microbiome. Taxonomic analysis at phylum level showed a significant increase in Bacteroidetes and Saccharibacteria (formally TM7) and decrease in Proteobacteria and Actinobacteria over time, in both plaque and saliva. Genus level analysis of relative abundance indicated a significant increase in anaerobic and facultative anaerobes in both plaque and saliva. Fixed orthodontic appliances induced measurable changes in the oral microbiome. This was characterized by an increase in relative abundance of obligate anaerobes, including periodontal pathogens. It can be concluded that this dysbiosis induced by fixed orthodontic appliances is likely to represent a transitional stage in the shift in microbiome from healthy to periodontitis.

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A single-centre investigator-blinded randomised parallel group clinical trial to investigate the effect of probiotic strainsStreptococcus salivariusM18 andLactobacillus acidophiluson gingival health of paediatric patients undergoing treatment with fixed orthodontic appliances: study protocol

BMJ Open ◽

10.1136/bmjopen-2019-030638 ◽

2019 ◽

Vol 9 (9) ◽

pp. e030638 ◽

Cited By ~ 2

Author(s):

Eleftherios G Kaklamanos ◽

Rania Nassar ◽

Sotirios Kalfas ◽

Manal Al Halabi ◽

Mawlood Kowash ◽

...

Keyword(s):

Treatment Group ◽

Oral Hygiene ◽

Oral Microbiome ◽

Control Group ◽

Orthodontic Appliances ◽

Single Centre ◽

Paediatric Patients ◽

Gingival Health ◽

Parallel Group ◽

Fixed Orthodontic Appliances

BackgroundThere is limited data on the beneficial effects of probiotics on the gingival health of patients undergoing treatment with fixed orthodontic appliances. This study aims to compare the effect of probiotic tablets combined with regular oral hygiene versus regular oral hygiene alone on gingival status in these patients. The effect of probiotic intake on plaque formation and salivary microbiome composition will be also assessed.Methods and analysisThis is a 3 month single-centre, single blind (clinical and laboratory examiners), parallel group randomised controlled two arm superiority trial. Fifty paediatric patients attending the Postgraduate Orthodontic Clinic at the Hamdan Bin Mohammed College of Dental Medicine (HBMCDM), Mohammed Bin Rashid University of Medicine and Health Sciences (MBRU), Dubai, United Arab Emirates, who meet the eligibility criteria will be recruited. Block randomisation with 1:1 allocation and concealment of allocation will be carried out. The treatment group will receive probiotic tablets containingStreptococcus salivariusM18 andLactobacillus acidophilustogether with regular oral hygiene versus the control group on regular oral hygiene alone. Clinical examination and collection of saliva for microbiome assay will be carried out at baseline and end of study. Self-reporting by patients will be used to document acceptability and adverse effects. Statistically significant decrease in gingival bleeding on probing in the treatment group will be classified as primary outcome of treatment success. Statistically significant reduction in Plaque Index, Gingival Index and shift in the composition of the oral microbiome in favour of beneficial bacteria are secondary outcomes indicative of efficacy of probiotic intake.Ethics and disseminationEthical approval for the study has been granted by the HBMCDM, MBRU, Institutional Review Board (Reference #: MBRU-IRB-2018–015). Study findings will be disseminated via publication in peer-reviewed journal.Trial registration numberISRCTN95085398

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