scholarly journals Efficient genetic transformation method for Eucalyptus genome editing

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0252011
Author(s):  
Zechen Wang ◽  
Limei Li ◽  
Lejun Ouyang
Keyword(s):  
Wood Quality ◽  
Eucalyptus Grandis ◽  
Transformation Method ◽  
Raw Material ◽  
Plant Genome ◽  
Forest Industry ◽  
Transgenic Technology ◽  
Pulp Paper ◽  
Alternative Approach ◽  
Labelling Method

Plantation forestry of Eucalyptus urophylla × Eucalyptus grandis supplies high-quality raw material for pulp, paper, wood, and energy and thereby reduces the pressures on native forests and their associated biodiversity. Nevertheless, owing to the heterozygosity of the E. urophylla × E. grandis genetic background, germplasm improvement by crossbreeding tends to be inefficient. As an alternative approach, genetic engineering of Eucalyptus can be used to effectively improve germplasm resources. From a strategic standpoint, increasing the plantation productivity and wood quality by transgenic technology has become increasingly important for forest industry. In this study, we established a fluorescence labelling method using CRISPR/Cas9 technology to obtain positive transformed progenies. The positive transformed progenies were easily obtained from the genetically modified population via fluorescence screening. This system can be used as a plant genome site-specific editing tool and may be useful for improving Eucalyptus genetic resources.

scholarly journals Quality of wood and pulp from a clone of Eucalyptus grandis planted at three locations

CERNE ◽  
2011 ◽  
Vol 17 (1) ◽  
pp. 47-60 ◽  
Author(s):  
Cláudio Angeli Sansígolo ◽  
Éder da Silva Ramos
Keyword(s):  
Wood Quality ◽  
Eucalyptus Grandis ◽  
Basic Density ◽  
Chemical Properties ◽  
Matter Content ◽  
Raw Material ◽  
Low Fertility ◽  
Dry Matter Content ◽  
Chemical Analyses

Eucalyptus grandis is one of the most popular species to use as raw material for pulp production in Brazil, as it provides excellent pulping, bleaching and papermaking results. The main objective of this study is to examine the quality of wood and pulp from a clone of Eucalyptus grandis, at age 4 years approximately, planted in three different soils, two of which being low fertility and one being a superior fertility soil, and their reflections on wood quality and pulping results. Chemical analyses of the soils followed guidelines developed by Raij et al. (2001), while physical and chemical properties of the wood followed ABTCP, TAPPI and ABNT standards. Chemical analyses of the soils pointed to lower fertility in Fazenda Limeira and Fazenda Tapera Queimada as opposed to higher fertility in Fazenda Mendes União. Results revealed stronger tendencies for the stand grown in the less fertile soils (Fazenda Limeira and Fazenda Tapera Queimada) in comparison to the stand grown in more fertile soil (Fazenda Mendes União) as to: higher wood and bark basic density, lower height and dry matter content, higher bark content, lower extractives content and higher holocellulose content, higher total and screened yields from pulping, lower specific wood consumption/t of pulp, and higher tear and tensile indices at low refining levels.

Behavior of xylans from the Eucalyptus species. Part 2. Characterization of 4-O-methylglucuronoxylans isolated from black liquors of kraft pulping of Eucalyptus grandis and E. urophylla

Holzforschung ◽  
2013 ◽  
Vol 67 (2) ◽  
pp. 123-128
Author(s):  
Andréia S. Magaton ◽  
Teresa Cristina F. Silva ◽  
Jorge Luiz Colodette ◽  
Dorila Piló-Veloso ◽  
Flaviana Reis Milagres ◽  
...  
Keyword(s):  
Average Molecular Weight ◽  
Eucalyptus Grandis ◽  
Kraft Pulping ◽  
Raw Material ◽  
Size Exclusion ◽  
Eucalyptus Species ◽  
Exclusion Chromatography ◽  
Ft Ir ◽  
Black Liquors

Abstract 4-O-methylglucuronoxylans isolated from Eucalyptus grandis and Eucalyptus urophylla kraft black liquors (KBLs) were chemically characterized by Fourier transform infrared spectroscopy (FT-IR), size exclusion chromatography (SEC), and nuclear magnetic resonance (NMR) spectroscopy. Doses of alkali charge, expressed as active alkali (AA), were 16, 17, and 18% while the sulfidity was kept at 25%. Kappa numbers of 19.1, 17.5, and 16.1 for E. grandis and 20.4, 16.8, and 15.4 for E. urophylla were obtained. At higher alkali charges, the recovery of xylans from the KBLs was lower and the degree of substitution of xylans with uronic acids decreased. The average molecular weight (Mw) of the recovered xylans was greater under conditions of mild pulping, i.e., in the case of pulps with higher kappa numbers. Mw of xylans ranged from 16.1 to 19.1 kDa for E. grandis and from 15.4 to 20.4 kDa for E. urophylla. The xylans from KBL may be useful as pulp modifying agents or as a raw material for advanced applications.

scholarly journals Soybean waste in particleboard production

2018 ◽  
Vol 42 (2) ◽  
pp. 186-194 ◽  
Author(s):  
Evelyn Hoffmamm Martins ◽  
Alan Pereira Vilela ◽  
Rafael Farinassi Mendes ◽  
Lourival Marin Mendes ◽  
Lívia Elisabeth Vasconcellos de Siqueira Brandão Vaz ◽  
...  
Keyword(s):  
Water Absorption ◽  
Water Immersion ◽  
Eucalyptus Grandis ◽  
Raw Material ◽  
Modulus Of Rupture ◽  
High Yield ◽  
Thickness Swelling ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Hybrid Eucalyptus

ABSTRACT Brazil is the second largest soybean producer in the world, with a yield of around 96.2 million tons per crop. This high yield leads to a great amount of waste resulting from soybean cultivation, which can reach approximately 41 million tons of waste per year. This material has lignocellulosic properties, which may enable its use as a raw material for particleboard production. Therefore, the objective of this study was to evaluate the use of soybean pods in particleboard production. For particleboard manufacture, wood of the hybrid Eucalyptus urophylla and Eucalyptus grandis was used, added with soybean pods, at proportions of 0%, 25%, 50%, 75% and 100%. For particleboard evaluation, a completely randomized design was used, with five treatments and three replicates, using linear regression and the Scott-Knott test at 5% significance for comparison among the different treatments. The properties apparent density, compaction ratio, water absorption after 2 and 24 hours, thickness swelling after 2 and 24 hours in water immersion, internal bonding, modulus of rupture and modulus of elasticity in bending properties were evaluated. The ratio soybean pod waste and eucalyptus particles in the panels led to an increase in water absorption values and thickness swelling, in addition to a decrease in mechanical properties. The production of panels with approximately 23% soybean pods is feasible.

scholarly journals Structural products made of beech wood: quality assessment of the raw material

2020 ◽  
Vol 78 (5) ◽  
pp. 961-970
Author(s):  
Michele Brunetti ◽  
Michela Nocetti ◽  
Benedetto Pizzo ◽  
Giovanni Aminti ◽  
Corrado Cremonini ◽  
...  
Keyword(s):  
Quality Assessment ◽  
Wood Quality ◽  
Beech Wood ◽  
Raw Material

Effects of the lateral growth rate on wood quality parameters of Eucalyptus grandis from different latitudes in Brazil and Argentina

2009 ◽  
Vol 257 (10) ◽  
pp. 2175-2181 ◽  
Author(s):  
Miho Kojima ◽  
Fabio Minoru Yamaji ◽  
Hiroyuki Yamamoto ◽  
Masato Yoshida ◽  
Takahisa Nakai
Keyword(s):  
Growth Rate ◽  
Wood Quality ◽  
Eucalyptus Grandis ◽  
Quality Parameters ◽  
Lateral Growth ◽  
Lateral Growth Rate

Raw material factors affecting the quota of structural wood in sawmill production

2019 ◽  
Vol 107 ◽  
pp. 124-130
Author(s):  
MAREK WIERUSZEWSKI ◽  
RADOSŁAW MIRSKI ◽  
ADRIAN TROCIŃSKI
Keyword(s):  
Raw Materials ◽  
Wood Quality ◽  
Strength Properties ◽  
Solid Wood ◽  
Wood Products ◽  
Raw Material ◽  
Factors Affecting ◽  
Research Technology ◽  
Main Indicator ◽  
Round Wood

Construction wood has to meet high requirements for loads capacity. Thanks to the selection and adequate classification of raw materials it is possible to decide how specific wood products can and should be used. The usage of solid wood for structural elements purposes has been precisely classified and specified in standardized requirements. Twelve classes of structural timber, ranging from C14 to C50, have been identified for softwood. Only part of Polish raw wood meets the standards of strength classes required in the building industry, as its strength corresponds to classes C24 and C30. A conclusion from research on the assortment structure of the raw materials in Poland was formed that it is particularly important to sort wood effectively according to its quality and intended use during processing. The material and strength efficiency is the main indicator of rational use of wood. The research resulted in setting the initial classification limits of selected round wood groups according to its strength. Properties determining the possibilities of obtaining a high quality product from the available raw wood material were taken into account in the research. Crucial factor was to select the proper research technology of determining dependencies between the applied research methodology and the defined wood quality, sorting class and strength class. The lower limit of availability of raw material with appropriate technical characteristics is determined during the selection of the research formula.

GENETIC IMPROVEMENT OF FIBER LENGTH AND COARSENESS BASED ON PAPER PRODUCT PERFORMANCE AND MATERIAL VARIABILITY – A REVIEW

IAWA Journal ◽  
2004 ◽  
Vol 25 (4) ◽  
pp. 401-414 ◽  
Author(s):  
Brian K. Via ◽  
Michael Stine ◽  
Todd F. Shupe ◽  
Chi-Leung So ◽  
Leslie Groom
Keyword(s):  
Specific Gravity ◽  
Wood Quality ◽  
Product Performance ◽  
Raw Material ◽  
Pulp Yield ◽  
Quality Trait ◽  
Paper Strength ◽  
Improvement Program ◽  
Tracheid Length ◽  
Strength And Stiffness

Improvement of specific gravity through tree breeding was an early choice made in the mid 20th century due to its ease of measurement and impact on pulp yield and lumber strength and stiffness. This was often the first, and in many cases, the only wood quality trait selected for. However, from a product standpoint, increased specific gravity has shown to lower many paper strength and stiffness properties and has been assumed to be directly attributable to increased fiber coarseness. As a result, it is currently not clear which fiber trait would best benefit a tree improvement program for paper products. This review found coarseness to be perhaps more important to paper strength and stiffness whereas tracheid length showed better promise from a breeding point of view due to its independence from specific gravity. However, both traits possessed strong heritability and influence on product performance and thus both would be beneficial to breed for depending on organizational goals and end product mix. The objective of this paper is to review and prioritize coarseness and tracheid length from both an end use and raw material perspective. To aid in prioritization, the variation, correlation, and heritability of both traits were reviewed along with significant genetic and phenotypic correlations. Variation trends within and between families as well as within a tree were reviewed.

scholarly journals BIOMASS AND ENERGY PRODUCTION AT SHORT ROTATION Eucalyptus CLONAL PLANTATIONS DEPLOYED IN RIO GRANDE DO NORTE1

2017 ◽  
Vol 41 (5) ◽  
Author(s):  
Maraísa Costa Ferreira ◽  
Rosimeire Cavalcante dos Santos ◽  
Renato Vinícius Oliveira Castro ◽  
Angélica de Cássia Oliveira Carneiro ◽  
Gualter Guenther Costa da Silva ◽  
...  
Keyword(s):  
Energy Demand ◽  
Wood Quality ◽  
Forest Biomass ◽  
Eucalyptus Grandis ◽  
Basic Density ◽  
Tree Height ◽  
Volumetric Growth ◽  
Energy Productivity ◽  
Short Rotation ◽  
Calorific Power

ABSTRACT This study aimed to estimate the biomass and energy of a clonal planting short rotation experiment with hybrids of Eucalyptus urophylla ST Blake x Eucalyptus grandis (Hill) Maiden, deployed in 3 m x 3 m spacing, at the Academic Unit Specialized in Agricultural Sciences, Macaíba/RN. The analysis were performed at the Laboratory Panels and Wood Energy, Federal University of Viçosa, in which the following ratings on wood three cultivars (GG100 ; AEC0144 and AEC0224) were performed: determination of basic density, high calorific power, chemical analysis, thermogravimetric analysis and subsequent calculations for estimating the mass production and energy were performed. The base (10 cm from the ground), the third and two thirds of commercial tree height: composed for analysis, taken from three points along the stem samples were used. The design of the experiment was a completely randomized. Data were subjected to analysis of variance and the means were compared by Tukey test (a= 0.05). The clone who excelled in the first year of planting was the GG100 with 11,805 kW.h.ha-1 of energy productivity, followed by clone AEC0224 (6,755 kW.h.ha-1) and AEC0144 (4,405 kW.h.ha-1). The performance of this clone was due to mainly the higher volumetric growth. The wood quality among clones showed little difference. It should stimulate the deployments of species of short rotation in the state of RN to supply the elevated energy demand of forest biomass in the region.

scholarly journals DAMAGE BY DEFOLIATING INSECTS AND ITS EFFECT ON THE QUALITY OF WOOD FOR CHARCOAL PRODUCTION

2021 ◽  
Vol 55 (7-8) ◽  
pp. 933-938
Author(s):  
ANTONIO JOSÉ VINHA ZANUNCIO ◽  
AMÉLIA GUIMARÃES CARVALHO ◽  
CARLOS MIGUEL SIMÕES DA SILVA ◽  
VINÍCIUS RESENDE DE CASTRO ◽  
ANGÉLICA DE CASSIA OLIVEIRA CARNEIRO ◽  
...  
Keyword(s):  
Wood Quality ◽  
Eucalyptus Grandis ◽  
Basic Density ◽  
Charcoal Production ◽  
Fixed Carbon ◽  
Eucalyptus Saligna ◽  
B Trees ◽  
The Impact ◽  
Holocellulose Content

The objective of the present work has been to evaluate the impact of damage caused by defoliating insects on wood quality and charcoal production, and to quantify the losses per hectare according to the charcoal produced. Seven-year-old Eucalyptus grandis × Eucalyptus urophylla (clone A) and Eucalyptus saligna (clone B) trees, both in healthy condition and damaged by defoliating insects, were selected, with five trees to be used per treatment. Wood disks were removed from the trees at 0, 25, 50, 75 and 100% of the commercial height for analyzing the properties of the wood and for preparing and characterizing the charcoal. Damage by defoliating insects decreased the basic density of the trees at all axial positions by up to 23 kg m-3. Also, the extractives and lignin contents increased, while the holocellulose content decreased in the attacked plants. Changes in the wood characteristics led to increased fixed carbon content and gravimetric yield, and a decrease in density. The charcoal productivity from the plants damaged by defoliating insects was lower, mainly because of the decrease in volumetric production.

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