scholarly journals Minimizing Matrix Effects for the Accurate Quantification of 25-Hydroxyvitamin D Metabolites in Dried Blood Spots by LC-MS/MS

2016 ◽  
Vol 62 (4) ◽  
pp. 639-646 ◽  
Author(s):  
David Kvaskoff ◽  
Alicia K Heath ◽  
Henry A Simila ◽  
Pauline Ko ◽  
Dallas R English ◽  
...  
Keyword(s):  
Vitamin D ◽  
Matrix Effects ◽  
Fold Increase ◽  
Dried Blood Spots ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
Multiple Organs ◽  
25 Hydroxyvitamin D ◽  
Increasing Demand ◽  
The Impact

Abstract BACKGROUND The noncalcemic actions of vitamin D in multiple organs are now widely recognized. Vitamin D status has been linked with a wide variety of conditions, which has led to an increasing demand for vitamin D screening. In particular, there is intense interest in the impact of vitamin D on a variety of developmental conditions. The most readily accessible pediatric samples are dried blood spots, and health organizations are increasingly archiving such samples for later assessment of the antecedents of disease. METHODS In 2009, we developed a method to quantify the major circulatory form of vitamin D, 25-hydroxyvitamin D, in archived dried blood spots. Over the last 6 years, we have made substantial alterations to the published method to enhance throughput, sensitivity, and assay robustness. RESULTS With the alterations, the assay was 3 times faster than the previously published assay and had a >10-fold increase in signal strength. Intraassay imprecision decreased from 13.4% to 6.9%, and there was a 5-fold reduction in interfering phospholipids. In actual use over 2 years, the assay showed an interassay imprecision of 11.6%. CONCLUSIONS This assay has performed reliably over the past 6 years. The practical changes we have made should allow clinical chemists to successfully adapt this method.

Simplified 25-Hydroxyvitamin D Standardization and Optimization in Dried Blood Spots by LC-MS/MS

2017 ◽  
Vol 100 (5) ◽  
pp. 1328-1336 ◽  
Author(s):  
Andrew J Makowski ◽  
John A Rathmacher ◽  
Ronald L Horst ◽  
Christopher T Sempos
Keyword(s):  
Vitamin D ◽  
Dried Blood Spots ◽  
Serum Concentrations ◽  
Vitamin D Binding Protein ◽  
Serum Samples ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Sample Representation ◽  
Concentration Assessment

Abstract Previous studies have assessed vitamin D status based on the 25-hydroxyvitamin D [25(OH)D] concentration measured in samples from dried blood spots (DBSs). In 40 individuals participating in a clinical study, we compared 25(OH)D levels measured from DBSs and in serum using an LC-MS/MS reference procedure in collaboration with the Vitamin D Standardization Program. The main objective was to simplify and optimize current methods to produce an assay that can be used as a screening tool for 25(OH)D concentration assessment without derivatization. The DBS 25(OH)D levels, compared to serum concentrations, were found to have 101% accuracy overall, and the correlation coefficient (r) was 0.83 (P < 0.0001), with a significant linear relationship. Free 25(OH)D and vitamin D binding protein (VDBP) were assessed in the serum samples for potential correlations to the DBS calculations: the levels of free 25(OH)D had moderate to strong correlation to DBS and serum concentrations, with r values of 0.67 (P < 0.0001) and 0.76 (P < 0.0001), respectively. VDBP and hematocrit had no significant correlation to either DBS or serum sample types, with r values <0.1. In conclusion, the useof two DBSs and an increase in DBS sample size improved overall sample representation without the need for derivatization, and produced an accurate and robust method that can be used to screen 25(OH)D levels.

scholarly journals Weight and skin colour as predictors of vitamin D status: results of an epidemiological investigation using nationally representative data

2016 ◽  
Vol 20 (10) ◽  
pp. 1857-1864 ◽  
Author(s):  
Sonali Rajan ◽  
Tom Weishaar ◽  
Bryan Keller
Keyword(s):  
Vitamin D ◽  
Body Weight ◽  
Skin Colour ◽  
Vitamin D Status ◽  
Epidemiological Investigation ◽  
Dietary Recommendations ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Nationally Representative ◽  
The Impact

AbstractObjectiveCurrent US dietary recommendations for vitamin D vary by age. Recent research suggests that body weight and skin colour are also major determinants of vitamin D status. The objective of the present epidemiological investigation was to clarify the role of age as a predictor of vitamin D status, while accounting for body weight and skin colour, among a nationally representative sample.DesignWe calculated the mean serum 25-hydroxyvitamin D levels for the US population by age and weight, as well as by weight and race/ethnicity group. Multiple regression analyses were utilized to evaluate age and weight as predictors of vitamin D status: serum 25-hydroxyvitamin D levels with age alone, age and body weight, and age, body weight and their two-way interaction were modelled for the entire sample and each age subgroup. Graphical data were developed using B-spline non-linear regression.SettingNational Health and Nutrition Examination Survey (31 934 unweighted cases).SubjectsIndividuals aged 1 year and older.ResultsThere were highly significant differences in mean vitamin D status among US residents by weight and skin colour, with those having darker skin colour or higher body weight having worse vitamin D status. Although a significant factor, the impact of age on vitamin D status was notably less than the impact of body weight.ConclusionsVitamin D status varied predominantly by body weight and skin colour. Recommendations by nutritionists for diet and supplementation needs should take this into account if vitamin D-related health disparities are to be meaningfully reduced across the USA.

scholarly journals The impact of vitamin D pathway genetic variation and circulating 25-hydroxyvitamin D on cancer outcome: systematic review and meta-analysis

2017 ◽  
Vol 116 (8) ◽  
pp. 1092-1110 ◽  
Author(s):  
P G Vaughan-Shaw ◽  
F O'Sullivan ◽  
S M Farrington ◽  
E Theodoratou ◽  
H Campbell ◽  
...  
Keyword(s):  
Systematic Review ◽  
Vitamin D ◽  
Genetic Variation ◽  
Meta Analysis ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Cancer Outcome ◽  
The Impact

scholarly journals Is the Climb Worth the View? The Savings/Alert Ratio for Reducing Vitamin D Testing

2020 ◽  
Vol 11 (01) ◽  
pp. 160-165
Author(s):  
Chase D. Hendrickson ◽  
Michael F. McLemore ◽  
Kathryn M. Dahir ◽  
Shari Just ◽  
Zahra Shajani-Yi ◽  
...  
Keyword(s):  
Vitamin D ◽  
Medical Center ◽  
Intervention Group ◽  
Clinical Decision ◽  
Control Group ◽  
Hydroxyvitamin D ◽  
Patient Demographics ◽  
25 Hydroxyvitamin D ◽  
A Charge ◽  
The Impact

Abstract Background Despite guideline recommendations, vitamin D testing has increased substantially. Clinical decision support (CDS) presents an opportunity to reduce inappropriate laboratory testing. Objectives and Methods To reduce inappropriate testing of vitamin D at the Vanderbilt University Medical Center, a CDS assigned providers to receive or not receive an electronic alert each time a 25-hydroxyvitamin D assay was ordered for an adult patient unless the order was associated with a diagnosis in the patient's chart for which vitamin D testing is recommended. The CDS ran for 80 days, collecting data on number of tests, provider information, and basic patient demographics. Results During the 80 days, providers placed 12,368 orders for 25-hydroxyvitamin D. The intervention group ordered a vitamin D assay and received the alert for potentially inappropriate testing 2,181 times and completed the 25-hydroxyvitamin D order in 89.9% of encounters, while the control group ordered a vitamin D assay (without receiving an alert) 2,032 times and completed the order in 98.1% of encounters, for an absolute reduction of testing of 8% (p < 0.001). Conclusion This CDS reduced vitamin D ordering by utilizing a soft-stop approach. At a charge of $179.00 per test and a cost to the laboratory of $4.20 per test, each display of the alert led to an average reduction of $14.70 in charges and of $0.34 in spending by the laboratory (the savings/alert ratio). By describing the effectiveness of an electronic alert in terms of the savings/alert ratio, the impact of this intervention can be better appreciated and compared with other interventions.

scholarly journals 25-Hydroxyvitamin D concentration and all-cause mortality: the Melbourne Collaborative Cohort Study

2016 ◽  
Vol 20 (10) ◽  
pp. 1775-1784 ◽  
Author(s):  
Alicia K Heath ◽  
Elizabeth J Williamson ◽  
David Kvaskoff ◽  
Allison M Hodge ◽  
Peter R Ebeling ◽  
...  
Keyword(s):  
Cohort Study ◽  
Cox Regression ◽  
Dried Blood Spots ◽  
Inverse Association ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
Excellent Health ◽  
Melbourne Collaborative Cohort Study ◽  
History Of ◽  
25 Hydroxyvitamin D

AbstractObjectiveTo investigate relationships between mortality and circulating 25-hydroxyvitamin D (25(OH)D), 25-hydroxycholecalciferol (25(OH)D3) and 25-hydroxyergocalciferol (25(OH)D2).DesignCase–cohort study within the Melbourne Collaborative Cohort Study (MCCS). We measured 25(OH)D2and 25(OH)D3in archived dried blood spots by LC–MS/MS. Cox regression was used to estimate mortality hazard ratios (HR), with adjustment for confounders.SettingGeneral community.SubjectsThe MCCS included 29 206 participants, who at recruitment in 1990–1994 were aged 40–69 years, had dried blood spots collected and no history of cancer. For the present study we selected participants who died by 31 December 2007 (n2410) and a random sample (sub-cohort,n2996).ResultsThe HR per 25 nmol/l increment in concentration of 25(OH)D and 25(OH)D3were 0·86 (95 % CI 0·78, 0·96;P=0·007) and 0·85 (95 % CI 0·77, 0·95;P=0·003), respectively. Of 5108 participants, sixty-three (1·2 %) had detectable 25(OH)D2; their mean 25(OH)D concentration was 11·9 (95 % CI 7·3, 16·6) nmol/l higher (P<0·001). The HR for detectable 25(OH)D2was 1·80 (95 % CI 1·09, 2·97;P=0·023); for those with detectable 25(OH)D2, the HR per 25 nmol/l increment in 25(OH)D was 1·06 (95 % CI 0·87, 1·29;Pinteraction=0·02). HR were similar for participants who reported being in good, very good or excellent health four years after recruitment.ConclusionsTotal 25(OH)D and 25(OH)D3concentrations were inversely associated with mortality. The finding that the inverse association for 25(OH)D was restricted to those with no detectable 25(OH)D2requires confirmation in populations with higher exposure to ergocalciferol.

scholarly journals Sensitive and Robust LC-MS/MS Assay to Quantify 25-Hydroxyvitamin D in Leftover Protein Extract from Dried Blood Spots

2021 ◽  
Vol 7 (4) ◽  
pp. 82
Author(s):  
Sanne Grundvad Boelt ◽  
Lars Melgaard ◽  
Marta Jadwiga Thorbek ◽  
Nadia Sara Jensen MacSween ◽  
John J. McGrath ◽  
...  
Keyword(s):  
Small Molecules ◽  
Dna Extraction ◽  
Neonatal Screening ◽  
Dried Blood Spots ◽  
Protein Extract ◽  
Blood Spots ◽  
Hydroxyvitamin D ◽  
25 Hydroxyvitamin D ◽  
Precision And Accuracy

Neonatal dried blood spots (DBS) provide a remarkable resource for biobanks. These microsamples can provide information related to the genetic correlates of disease and can be used to quantify a range of analytes, such as proteins and small molecules. However, after routine neonatal screening, the amount of DBS sample available is limited. To optimize the use of these samples, there is a need for sensitive assays which are integrated across different analytic platforms. For example, after DNA extraction, protein extracts are available for additional analyses. We describe a sensitive and robust LC-MS/MS method for 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 optimized for leftover protein extracts from DBS, which has excellent recovery, precision, and accuracy.

scholarly journals The Impact of Sample Type on Vitamin D Quantification and Clinical Classification during Pregnancy

Nutrients ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3872
Author(s):  
Soriah M. Harvey ◽  
Vanessa E. Murphy ◽  
Peter G. Gibson ◽  
Michael Clarke ◽  
Megan E. Jensen
Keyword(s):  
Vitamin D ◽  
Pearson Correlation ◽  
Intraclass Correlation ◽  
Plasma Concentrations ◽  
Sample Type ◽  
Vitamin D Status ◽  
Hydroxyvitamin D ◽  
Significant Difference ◽  
25 Hydroxyvitamin D ◽  
The Impact

Measurement of vitamin D status has significant use in clinical and research settings, including during pregnancy. We aimed to assess the agreement of total 25-hydroxyvitamin D (25(OH)D) concentration, and its three analytes (25-hydroxyvitamin D3 (25(OH)D3), 25-hydroxyvitamin D2 (25(OH)D2) and Epi-25-hydroxyvitamin D3 (Epi-25(OH)D3)), in plasma and serum samples collected during pregnancy, and to examine the proportion of women who change vitamin D status category based on sample type. Matching samples were collected from n = 114 non-fasting women between 12–25 weeks gestation in a clinical trial in Newcastle, Australia. Samples were analysed by liquid chromatography-tandem mass-spectrometry (LC-MS/MS) to quantify total 25(OH)D and its analytes and examined using Bland-Altman plots, Pearson correlation (r), intraclass correlation coefficient and Cohen’s Kappa test. Serum total 25(OH)D ranged from 33.8–169.8 nmol/L and plasma ranged from 28.6–211.2 nmol/L. There was a significant difference for total 25(OH)D based on sample type (measurement bias 7.63 nmol/L for serum vs plasma (95% Confidence Interval (CI) 5.36, 9.90, p ≤ 0.001). The mean difference between serum and plasma concentrations was statistically significant for 25(OH)D3 (7.38 nmol/L; 95% CI 5.28, 9.48, p ≤ 0.001) and Epi-25(OH)D3 (0.39 nmol/L; 95% CI 0.14, 0.64, p = 0.014). Of 114 participants, 28% were classified as vitamin D deficient (<50 nmol/L) or insufficient (<75 nmol/L) based on plasma sample and 36% based on serum sample. Nineteen (16.7%) participants changed vitamin D status category based on sample type. 25-hydroxyvitamin D quantification using LC-MS/MS methodology differed significantly between serum and plasma, yielding a higher value in plasma; this influenced vitamin D status based on accepted cut-points, which may have implications in clinical and research settings.

Vitamin D supply among healthy blood donors in County Vas, Hungary

2012 ◽  
Vol 153 (41) ◽  
pp. 1629-1637 ◽  
Author(s):  
Éva Virágh ◽  
Dóra Horváth ◽  
Zoltán Lőcsei ◽  
László Kovács ◽  
Rita Jáger ◽  
...  
Keyword(s):  
Vitamin D ◽  
Parathyroid Hormone ◽  
Oral Contraceptives ◽  
Blood Donors ◽  
Elderly Subjects ◽  
Intact Parathyroid Hormone ◽  
Hydroxyvitamin D ◽  
Vitamin D Levels ◽  
25 Hydroxyvitamin D ◽  
The Impact

Introduction: There is growing evidence showing the importance of adequate vitamin D supply for preserving health. Aim: The aim of the study was to evaluate the vitamin D supply among healthy blood donors and healthy elderly subjects in County Vas, Hungary. Methods: Serum 25-hydroxyvitamin D, intact parathyroid hormone, calcium and albumin (Cobas, Modular, Roche), as well as serum alfa-2-globulin concentrations (Gelelfo, Interlab) were determined in 178 serum samples (68 men, 110 women, 41 were taking oral contraceptives). The results were analysed according to sex and age (younger and older than 43 years), and the impact of oral contraceptive use was also taken into consideration. Results: Deficiency and insufficiency in vitamin D levels were detected in 9.6% and 32% of the studied subjects, respectively, whereas sufficient vitamin D levels were present in 58.4% of the subjects. 63% of the older and 41.2% of the younger group had suboptimal vitamin-D supply (p < 0.01). In women taking oral contraceptives serum 25-hydroxyvitamin D and alfa-2-globulin levels were significantly higher, whereas serum albumin and calcium levels were lower than in the control group. There was no difference in serum intact parathyroid hormone concentration between oral anticoncipient users and non-users. Conclusions: The occurrence of suboptimal vitamin D supply is significant, although less frequent than that in literature reports. In women taking oral contraceptives, serum 25-hydroxyvitamin D levels were higher, but serum intact parathyroid hormone concentrations were not decreased suggesting that the increased 25-hydroxyvitamin D levels may be the consequence of oestrogen-induced alterations of serum protein fractions. Orv. Hetil., 2012, 153, 1629–1637.

Sign in / Sign up

Export Citation Format

Share Document