scholarly journals Effects of a standardized 40% ellagic acid pomegranate (Punica granatum L.) extract on seminiferous tubule histopathology, diameter, and epithelium thickness in albino Wistar rats after heat exposure

2019 ◽  
Vol 12 (8) ◽  
pp. 1261-1265
Author(s):  
Budi Utomo ◽  
Nurfitri Rahmah Daningtia ◽  
Gandul Atik Yuliani ◽  
Wiwik Misaco Yuniarti
Keyword(s):  
Ellagic Acid ◽  
Seminiferous Tubule ◽  
Wistar Rats ◽  
Punica Granatum ◽  
Histological Evaluation ◽  
Seminiferous Tubules ◽  
Acid Extract ◽  
Epithelium Thickness ◽  
Punica Granatum L ◽  
Tubule Diameter

Background and Aim: It has long been known that the spermatogenic tissue is very sensitive to temperatures higher than its physiologic temperature and causing cessation of activity and resulting in sterility. This study investigated the effect of a standardized 40% ellagic acid extract of pomegranate on the histopathology, diameter, and epithelial thickness of seminiferous tubules in albino rats exposed to heat. Materials and Methods: Twenty-five male albino Wistar rats were randomized at 7-8 months of age to five treatment groups. Group C was not treated; Group T0 was treated with 0.5% of Na carboxymethyl cellulose (CMC) 2 ml/day and exposed to heat. T1, T2, and T3 were treated with 75, 150, and 300 mg/kg/day of a standardized 40% ellagic acid extract of pomegranate (Punica granatum L.), respectively. The animals were orally administered Na CMC or pomegranate extract and were exposed to sunlight for 15 min at 40°C-42°C for 14 days. The animals were sacrificed on day 15 and the testes were removed for histological evaluation and measurement of seminiferous tubule diameter and epithelium thickness. Results: The diameter of seminiferous tubules from rats exposed to heat and treated with 300 mg/kg/day pomegranate extract was larger and the epithelia thicker than those in the other groups (p= <0.05). The protective effects of the standardized 40% ellagic acid extract may have been mediated by its antioxidant activity. Conclusion: Compared with controls, administration of 300 mg/kg/day of a standardized 40% ellagic acid extract of P. granatum L. for 14 days increased seminiferous tubule diameter and epithelium thickness in albino Wistar rats exposed to heat. Keywords: diameter, epithelium thickness, heat, pomegranate extract, seminiferous tubule.

scholarly journals Protective effects of curcumin against methotrexate-induced testicular damage in rats by suppression of the p38-MAPK and nuclear factor-kappa B pathways

2021 ◽  
Author(s):  
Leyla Kilinc ◽  
Yesim Hulya Uz
Keyword(s):  
Body Weight ◽  
P38 Mapk ◽  
Seminiferous Tubule ◽  
Nuclear Factor Kappa B ◽  
Seminiferous Tubules ◽  
Control Group ◽  
Nuclear Factor ◽  
Testicular Damage ◽  
Space Width ◽  
Tubule Diameter

Objective: Methotrexate (MTX), is a commonly used chemotherapeutic agent. This study aimed to investigate the possibility that curcumin (CMN) protects against MTX-induced testicular damage by affecting the phospho (p)-p38 mitogen-activated protein kinase (MAPK) and nuclear factor-kappa B (NF-kB) signaling pathways. Methods: Male Wistar albino rats were subdivided into three groups. The control group was given an intragastric (ig) administration of dimethyl sulfoxide (DMSO) daily for 14 days, the MTX group was given a single intraperitoneal (ip) dose of MTX (20 mg/kg) on the 11th day, and the MTX+CMN group was given ig CMN (100 mg/kg/day, dissolved in DMSO) for 14 days and a single ip dose of MTX (20 mg/kg) on the 11th day. Results: The animal weights, the seminiferous tubule diameter, and germinal epithelium height significantly decreased in the MTX group compared to the control group. The testes weight and the ratio of the testes to body weight did not change, whereas the number of seminiferous tubules and the interstitial space width increased significantly in the MTX group. The number of phospho-p38 (p-p38) MAPK immunopositive cells and the immunoreactivity of NF-kB also increased in the MTX group compared to the control group. Conclusion: CMN prevented the MTX-induced decreases in the body weight, seminiferous tubule diameter, and the germinal epithelium height, while significantly reducing the number of histologically damaged seminiferous tubules and the interstitial space width changes due to MTX. CMN also reduced the number of p-p38 MAPK immunopositive cells and the NF-kB immunoreactivity.

scholarly journals Effect of Laserpuncture Shoot on Reproduction Point of Male Mojosari Duck (Anas Platyrhynchos) on The Numbers of Spermatogonium Cells and Seminiferous Tubules Diameter

2017 ◽  
Vol 3 (6) ◽  
pp. 718
Author(s):  
Melyandari Ayu Qomar ◽  
Rimayanti Rimayanti ◽  
Tri Nurhajati
Keyword(s):  
Seminiferous Tubule ◽  
Anas Platyrhynchos ◽  
Research Result ◽  
Seminiferous Tubules ◽  
Group Iii ◽  
Randomized Design ◽  
Group I ◽  
Histological Slide ◽  
Completely Randomized Design ◽  
Tubule Diameter

The Objective of this research was to know the effect of laserpuncture on reproduction point of male Mojosari duck on the number of spermatogonium cells and seminiferous tubule diameter of the testes. This research using completely randomized design with eighteen male Mojosari ducks which divided into three group randomly. Group I (P0) without any treatment of laserpuncture, Group II (P1) with 0.2 J dose of laserpuncture, and Group III (P2) with 0.4 J dose of laserpuncture. Laserpuncture shooting was done on reproduction point with three days interval in one month. On the thirty-first day, the duck was dissected so that the testes can be taken then made by the histological slide of testes. The obtained data processed by Analysis of Variant (ANOVA) followed with Duncan. Research result showed that laserpuncture shoot on the reproduction point of five-month-old male Mojosari duck decreases the number of spermatogonium cells and seminiferous tubule diameter of the testes. Keyword: laserpuncture, Mojosari duck, spermatogonium, seminiferous tubule

scholarly journals Stability of the Ellagitannin Fraction and Antioxidant Capacity of Varietal Pomegranate Juices

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000
Author(s):  
Pedro Mena ◽  
Cristina García-Viguera
Keyword(s):  
Antioxidant Capacity ◽  
Ellagic Acid ◽  
Phenolic Content ◽  
Punica Granatum ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Promising Alternative ◽  
The Stability ◽  
Punica Granatum L

This work aimed to assess the effect of combining two pomegranate (Punica granatum L.) cultivars at different rates on the ellagitannin content, antioxidant capacity, and total phenolic content of varietal pomegranate juices. Widely distinct juices made from Mollar de Elche and Wonderful cultivars were used for the elaboration of blended juices. They were stored for 70 days at both room and refrigeration temperatures. This study revealed a significant cultivar effect on the stability of main pomegranate ellagitannins (punicalagins, punicalins, punicalagin-like compound, and ellagic acid derivatives) and on the antioxidant capacity measured by the ABTS+ and DPPH• in vitro assays. Blended juices enhanced and/or retained the initial ellagitannin content and antioxidant capacity of pure juices during storage. Thus, blending varietal juices could be suggested as a promising alternative to the development of fresh juices with a high, stable phytochemical load.

The effect of Achillea millefolium extract on spermatogenesis of male Wistar rats

2010 ◽  
Vol 30 (4) ◽  
pp. 328-334 ◽  
Author(s):  
Nasrin Takzare ◽  
Mir-Jamal Hosseini ◽  
Seyedeh Hamideh Mortazavi ◽  
Sahar Safaie ◽  
Rayhaneh Moradi
Keyword(s):  
Adult Male ◽  
Seminiferous Tubule ◽  
Wistar Rats ◽  
Sperm Count ◽  
Basal Membrane ◽  
Achillea Millefolium ◽  
Seminiferous Tubules ◽  
Native Plant ◽  
Cell Accumulation ◽  
Male Wistar Rats

Achillea millefolium or yarrow, a native plant in many countries, has been recognized in historical medicine, mainly because of its astringent effects. However, some aspects of the toxicity of yarrow such as possible effects on male reproductive system are not well established. In this investigation, the effects of A. millefolium L. extract on spermatogenesis in adult male wistar rats were studied. Eighty-five male Wistar rats were divided into nine experimental groups (10 in each group except the ninth group). Extract was administered at the dose of 200,400 and 800 mg/kg/day by intraperitoneal (IP) injection or through gavage for 22 days, on every other day. Three groups were determined as sham and control groups. Five rats from each group were killed and the rest of the rats were kept for 40 days later, but with no injection, to assess the reversibility of extract effect on spermatogenesis. The results of the study showed scattered immature cells on basal membrane in seminiferous tubules at the dose of 400 mg/kg/day IP. Moreover, a significant decrease in cell accumulation and vacuolization in seminiferous tubule was seen. At the dose of 800 mg/kg, IP, thickened seminiferous tubules on basal membrane, decrease in cell accumulation in seminiferous tubule, severe disarrangement, degenerative cells and severe decrease in sperm count were seen. At the dose of 800 mg/kg/day, orally, basal membrane was thickened and the disarrangement in cells was demonstrated. As a conclusion, our results suggest that the total extract of A. millefolium L. exhibit temporary antifertile activity in adult male animals.

scholarly journals EFFECT OF LYOPHILIZED JUICE OF PUNICA GRANATUM L. IN ANTINOCICEPTIVE AND INFLAMMATORY PAIN

2021 ◽  
pp. 17-19
Author(s):  
JOSE ANTONIO GUERRERO-SOLANO ◽  
MIRANDELI BAUTISTA ◽  
NELLY DEL SOCORRO CRUZ-CANSINO ◽  
ALEJANDRO CHEHUE-ROMERO ◽  
OSMAR ANTONIO JARAMILLO-MORALES
Keyword(s):  
Body Weight ◽  
Acetylsalicylic Acid ◽  
Inflammatory Pain ◽  
Wistar Rats ◽  
Formalin Test ◽  
Antinociceptive Effect ◽  
Punica Granatum ◽  
Reference Drug ◽  
Male Wistar Rats ◽  
Punica Granatum L

Objective: The aim of this study was to explore the analgesic activity of lyophilized juice of Punica granatum L., obtained from Hidalgo, Mexico, in theformalin test.Methods: We extracted the juice manually, filtered it and then dried down in a lyophilizer machine. We evaluated the antinociceptive effect oflyophilized juice from pomegranate in the formalin test (2%) in male Wistar rats (180–200 g body weight). Thirty minutes before the test, a dose of316 mg/kg (lyophilized juice) and acetylsalicylic acid as reference drug (100 mg/kg) both were administered intragastrically (i.g.).Results: The oral administration of lyophilized juice of pomegranate showed a significant decrease in the number of flinches in the temporal courseand a significant antinociceptive effect in nociceptive and inflammatory pain compared with the vehicle. In the same way, this effect appeared with thedrug of reference (acetylsalicylic acid 100 mg/kg i.g). Furthermore, it was shown that juice had a 34% of antinociception on overall effect versus vehicle.Conclusion: The results suggest that lyophilized juice of pomegranate has antinociceptive effect in nociceptive and inflammatory pain. Therefore, thisstudy supports the possible use of this lyophilized juice of pomegranate in the treatment of pain.

scholarly journals Testicular features of semi-confined Nellore bulls subjected to immunocastration

2020 ◽  
Vol 57 (4) ◽  
pp. e171210
Author(s):  
Luciana da Silva Leal Karolewski ◽  
Valmir Fernandes ◽  
Leonardo Acácio Ferreira de Souza ◽  
Gustavo de Freitas Baise ◽  
Ranulfo Piau Júnior ◽  
...  
Keyword(s):  
Seminiferous Tubule ◽  
Testicular Development ◽  
Scrotal Circumference ◽  
Microscopic Features ◽  
Epithelium Thickness ◽  
Length Width ◽  
Student’S T ◽  
The Right ◽  
Tubule Diameter ◽  
Student’S T Test

This study aimed to evaluate the effect of immunocastration on the scrotal circumference, as well as in the macroscopic and microscopic testicular features in Nellore bulls bred under a semi-confining system for 91 days. Sixty animals were divided into two groups: 30 intact animals (non-vaccinated) and 30 immunocastrated animals. The immunocastrated animals were treated with two anti-GnRH vaccine doses (BoprivaTM - Zoetis Australia Pty Ltd). The scrotal circumference (SC) was measured on days 0 and 56. Testicular parameters: biometry traits (length, width, and height – cm; volume – cm3 ) and weight (g) was measured on day 91. Three testicles from each group were histologically processed to find the seminiferous tubule diameter and epithelium thickness (µm). Data were analyzed in the Minitab® 19 statistical software. The macroscopic features (SC, and testes weight, volume, length, width, and height) and the microscopic features (seminiferous tubule diameter and epithelium thickness) were compared between the two groups through Student’s t-test and Mann-Whitney test. All the values were lower (p<0.05) in animals immunized against GnRH, except for the right testis width and seminiferous tubule diameter. Results indicate that immunization against GnRH affected testicular development.

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