scholarly journals Physiological status of broiler chickens with diets supplemented with milk thistle extract

2021 ◽  
pp. 1319-1323
Author(s):  
Olga Bagno ◽  
Sergey Shevchenko ◽  
Antonina Shevchenko ◽  
Oleg Prokhorov ◽  
Anna Shentseva ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Broiler Chickens ◽  
Clinical Chemistry ◽  
Current Trend ◽  
White Blood Cells ◽  
Control Group ◽  
Physiological Status ◽  
Milk Thistle ◽  
Wing Vein

Background and Aim: In recent decades, the use of various feed supplements is the current trend in poultry farming, among which phytogenics serve as alternatives to feed antibiotics. This study aimed to examine the effect of feeding various doses of milk thistle extract (Silybum marianum) on the morphological and biochemical parameters of the blood in broiler chickens. Materials and Methods: Experiments were carried out in an industrial poultry farm on broiler chickens of the Hubbard ISA F15 cross for 40 days. One control group and five experimental groups of day-old chickens were formed. The number of birds in each group was 50. Broilers of all groups received complete feed, and the experimental groups received an additional milk thistle extract at doses of 0.1, 0.5, 1.0, 1.5, and 2.0 mg/kg of body weight. Milk thistle medicinal plant extract was obtained using water-ethanol extraction followed by low-temperature vacuum drying. For the assessment of blood analyses, samples were collected from the wing vein of six chickens per group. Using unified methods recommended by the International Federation of Clinical Chemistry, the content of red blood cells, hemoglobin, white blood cells, total protein, protein fractions, triglycerides, glucose, calcium, phosphorus, and the concentration of alanine aminotransferase and aspartate aminotransferase in the blood serum were determined. Results: It was found that the introduction of milk thistle extract into the diet of broiler chickens with the aforementioned doses increased the number of red blood cells, hemoglobin, white blood cells in the blood, as well as a decrease in the level of albumin and an increase in the content of γ-globulins in its serum. Conclusion: The authors assume that the introduction of milk thistle extract into a complete feed for broiler chickens increased the anabolic processes in their bodies, accompanied by increased use of proteins of the albumin fraction as the main material for organogenesis.

scholarly journals Effectiveness of Bacillus subtilis (Vitafort) probiotic used for raising broiler chickens

2022 ◽  
Vol 78 (02) ◽  
pp. 6621-2022
Author(s):  
RIFKAT FAIRUSHIN ◽  
SVETLANA SHAKIROVA ◽  
RIMMA GANIEVA ◽  
GALIYA SHAKIROVA
Keyword(s):  
Bacillus Subtilis ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Broiler Chickens ◽  
White Blood Cells ◽  
Live Weight ◽  
The Body ◽  
Farm Animals ◽  
Control Group ◽  
Daily Growth

Unbalanced nutrition as well as diseases of infectious and non-infectious etiology lead to a decrease in production and a significant loss of farm animals and birds. To mitigate these problems, various probiotic drugs (probiotics) are introduced into the diets of birds and animals for preventive purposes. The aim of the research was to study the effect of the Vitafort probiotic, containing antagonistic bacteria of the endophytic strain Bacillus subtilis B11, on the body of broiler chickens. The effect of Vitafort was assessed by measuring the live weight and average daily growth of birds. In addition, a morphological study of blood was carried out and the content of Bacillus subtilis in the small intestine was determined. It was found that the endophytic strain Bacillus subtilis B11 improved the intestinal microflora. Thus, on day 5 of its use, the number of microorganisms varied from 4.1 × 104 to 8.2 × 104 CFU/g, and on day 42 the same number varied from 4 × 108 to 5.8 × 108 CFU/g on day 42, the contents of red blood cells, haemoglobin, and white blood cells in the blood of chickens from the experimental group were higher by 6.44%, 3.44%, and 2.11%, respectively, than they were in the control group. Considering the above facts, it can be assumed that the absorption of nutrients in the intestine improved, and metabolic processes were activated due to an increase in the transport abilities of red blood cells.

scholarly journals BIOLOGICAL EFFECTS OF SILICON OXIDE NANOPARTICLES ON BROILER CHICKEN

2020 ◽  
Vol 5 (2) ◽  
pp. 63-71
Author(s):  
Vladimir Nikulin ◽  
Aleksandra Mustafina
Keyword(s):  
Red Blood Cells ◽  
Total Protein ◽  
Blood Cells ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Silicon Oxide ◽  
The Body ◽  
Control Group ◽  
The Third ◽  
Experimental Group

The aim of the study is to increase the productive qualities of broiler chickens by including ultrafine silicon oxide into main diet. During the experiment, the biological effect of ultrafine silicon oxide on broiler chickens was estab-lished. Use of ultrafine SiO2 particles for poultry feeding contributed to an increase in the number of red blood cells and content of total protein and albumins. By the end of the experiment, the number of red blood cells in birds in-creased by 17.43% (P≤0.001) – in the blood of birds of the first experimental group, 16.51% (P≤0.01) – the second one, 20.80% (P≤0.001) – the third experimental and 21.71% (P≤0.001) – the fourth experimental group, compared with the indicator of the control group. The amount of total protein in blood serum of chickens of the first and the second experimental groups increased by 1.36-1.39 %, in the third and fourth ones there was a significant (P≤0.05) increase by 5.45 and 3.05%, respectively. The blood glucose content of chickens in the experimental groups is higher by 8.04-23.65% compared to this indicator with ones in the control group. During the experiment feed con-sumption per 1 kg gain of live weight decreased: in the first experimental group by 3.00 % in the second by – 0.50 %, the third – 6.00 % the fourth– by 4.50 %, compared to this with the control group. The chicken’s vibrancy of the first the experimental group was higher by 4.77 %, the second – by 6.20 %, the third – by 19.25 % and the fourth– by 11.59% than in the control one. Consequently, when converting the feed energy into the body energy of a broiler chicken, the energy conversion coefficient of the experimental group of poultry is higher than that of the control one by 7.16-21.76 %. Thus, the most optimal dose for further research was determined.

scholarly journals Evaluation of Hematocrit Level, Red Blood Cells and White Blood Cells Counts in Blood from Patients with Different Severities of Periodontal Diseases

2018 ◽  
Vol 30 (3) ◽  
pp. 1-6
Author(s):  
Chinar J Ali ◽  
Maha A Ahmed
Keyword(s):  
Chronic Disease ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Periodontal Diseases ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Control Group ◽  
Pocket Depth ◽  
Anemia Of Chronic Disease ◽  
Mean Values

Background: Anemia of chronic disease (ACD) occurs in the presence of chronic infection, inflammatory conditions or neoplastic conditions despite of adequate iron and vitamins storage. Gingivitis is the inflammation of the gingiva, periodontitis is the inflammation in the periodontium that extend deeper with loss of connective tissue attachment and supporting bone. The main pathogenesis of periodontal diseases and ACD is immune activation. Aims of study: Determine and compare the clinical periodontal parameters (plaque index (PLI), gingival index (GI), bleeding on probing (BOP), probing pocket depth (PPD) and clinical attachment level (CAL)). Evaluate the hematocrit (Hct) level, red blood cells (RBCs) count and white blood cells (WBCs) count. Assess the correlations between the clinical periodontal parameters and hematological parameters at patients had gingivitis, chronic periodontitis (CP) with different severities (mild, moderate and severe) with healthy periodontium subjects. Materials and Methods: 35-50 years old, 150 male subjects were included in this study. They were divided into three study groups: group of 30 patients with gingivitis, group of 90 patients with CP which subdivided into (Mild CP=30 patients, Moderate CP =30 patients, Severe CP =30 patients) and control group 30 subjects with clinically healthy periodontium. Blood samples were collected then by automated blood analyzer the Hct, RBCs and WBCs were evaluated. Results: Comparisons among groups and subgroups revealed significant differences in Hct and WBCs, while RBCs was non-significant. Means values of RBCs count showed reduction in mild and severe CP subgroups. while, the Hct and WBCs mean values increased in patients with periodontal disease. The correlations between the clinical periodontal parameters with WBCs and RBCs were almost non-significant but, with Hct was mostly significant negative correlations. Conclusion: Inflammatory and immune responses in periodontal diseases caused change in different hematologic parameters which could contribute to the development of anemia of chronic disease.

scholarly journals Haematological and Genotoxic effects of cadmium chloride on Mesopotamichthys sharpeyi

2016 ◽  
Vol 13 (4) ◽  
pp. 745-752
Author(s):  
Baghdad Science Journal
Keyword(s):  
Red Blood Cells ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Blood Cells ◽  
Cadmium Chloride ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Blood Picture ◽  
Genotoxic Effects

The present study investigated Haematological changes in Mesopotamichthys sharpeyi, as well as determination genotoxic effects of cadmium chloride on bunni fish by using 120 fingerlings, fish were distributed randomly into four treatments in addition to control group. Fish in first group treated (T1) with cadmium 0.093mg/L with changing water and added cadmium continuously, fish in the second group treated (T2) with cadmium 0.093mg/L with changing water without adding cadmium, third treatment (T3) with cadmium 0.046mg/L with changing water and adding cadmium continuously, and fourth treatment (T4) with cadmium 0.046mg/L with changing water without adding cadmium. Results of blood picture in T1 and T3 showed a significant reduction in red blood cells count, hemoglobin concentration, packed cell volume values, while the number of white blood cells showed a significant increase in its values. Results showed presence of improvement of clinical and microscopical signs and blood picture in T2 and T4, were changed water aquarium continuously and added cadmium only once compared withT1 and T3. Results of the present study concluded that changing water aquarium in the treatments without adding cadmium led to improvement of health status of fish which increased with the passage of time results of blood picture were almost the same of the control group. It could be concluded from the current study that the adding of cadmium to water aquarium containing bunni fish led to decrease in red blood cells count, hemoglobin and packed cell volume values and increase in micronuclei number.

scholarly journals Study of blood levels of Lactate, Vitamin D and Nucleated Red Blood Cells for early prediction of Hypoxic–Ischemic Encephalopathy: A case-control study

2021 ◽  
Author(s):  
Ahmed Hosni Saad ◽  
Ahmed Thabet Mahmoud ◽  
Mohamed Abdelrehem Soliman ◽  
Alyaa Ahdy Abdelaziz
Keyword(s):  
Vitamin D ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Venous Blood ◽  
White Blood Cells ◽  
Control Group ◽  
Blood Levels ◽  
P Value ◽  
Nucleated Red Blood Cells ◽  
Control Study

Abstract Objective and background: Perinatal asphyxia is a serious medical condition in which there is lack of oxygen or blood flow during, or even after the labor process. So, this study aimed to study serum blood levels of Lactate, vitamin D and count of nucleated Red Blood Cells (nRBCs) per 100 White Blood Cells (WBCs) as early biological predictors of Hypoxic–Ischemic Encephalopathy (HIE) after birth Asphyxia.Methods: A retrospective case-control study was conducted on 20 neonates with HIE comprising the cases (APGAR ≤ 5) and another 20 neonates representing the control group with their age, sex, and weight matched, admitted to NICU from March 2020 to the end of September 2020 at Menoufia University Hospital and El-Bagour Central Hospital, Egypt. Venous blood samples for lactate, vitamin D and nucleated RBCs were drawn during 1st 6h of birth and sent for analysis. Sarnat score was used to assess the severity of HIE.Results: Venous blood levels of lactate and nucleated Red Blood Cells per 100 White Blood Cells (nRBC/100 WBC) counts were significantly higher in HIE neonates than in the control group with a highly significant difference (P-value < 0.001). While serum Vitamin D was significantly lower in the HIE group than in the control (P-value < 0.001).Conclusion: Serum lactate level, vitamin D level and nRBCs/100 WBCs might be used as biomarkers in the early prediction of HIE.

scholarly journals The eff ect of poliodoxin and tilmox 25% on the morphological and biochemical parameters of the blood of broiler chickens

2020 ◽  
pp. 72-79
Author(s):  
A. Tyshkivska ◽  
V. Dukhnitsky ◽  
M. Tyshkivsky
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Clinical Condition ◽  
Biochemical Parameters ◽  
Blood Test ◽  
Broiler Chickens ◽  
White Blood Cells ◽  
Research Groups ◽  
Hemoglobin Content ◽  
Behavioral Reactions

The article presents the results of studies of the eff ect of the drug “Poledoxin”, which belongs to the group of tetracyclines and the drug “Tilmox 25%”, belonging to the group of macrolides, on some morphological and biochemical parameters of blood of Coobb-500 broiler chickens. It has been established that the use of the preparations “Poledoxin” and “Tilmox 25 %” for healthy broiler chickens according to the scheme recommended for treating birds with ornithobacteriosis and infections complicated by the causative agent of ornithobacteriosis does not cause changes in the clinical condition and behavioral reactions of the bird. A blood test found that under the infl uence of the drug “Poledoxin”, the number of red blood cells in the blood of broiler chickens increased by 4–59 % (p< 0,01) in chickens which used the drug “Tilmox 25 %” – by 5–46 % relative to control. The hematocrit value was less than the control indicator by 8 % in chickens that were treated with Poledoxin after 6 days of experiment (p< 0,05) in chickens that were used with tilmox 25 % – by 10 % (p< 0,05) through 6 and 9 days of experience. The hemoglobin content in the blood of poultry of the studied groups one day after drinking antibiotic solutions was less than the control by 7 and 8 %, respectively (p< 0,05, p< 0,01), and after 4 days – by 11 and 10 % (p< 0,01). Under the infl uence of Pledoxin, the volume of 1 erythrocyte (MCV, μm 3) in the blood of chickens was less than the control by 23–39 % (p< 0,05, p< 0,01), and when exposed to tilmox 25% – by 16–35 % (p< 0,01). The hemoglobin content in 1 erythrocyte (MCH, pg) was 62–83 % of the control index when exposed to Poledoxin (p< 0,01) and 69–86 % when exposed to tylmox 25 % (p< 0,01). The number of leukocytes in the blood of a bird under the infl uence of “Poledoxin” decreased by 8 % after a day (p< 0,01), and increased by 6 and 7 % after 3 and 4 days (p< 0,01). A decrease in the number of leukocytes in the blood of chickens of both research groups by 5–11 % (p< 0,001, p< 0,01) was established after 8 and 9 days. Key words: Poledoxin, Tilmox 25 %, broiler chickens, red blood cells, hematocrit, hemoglobin, white blood cells.

scholarly journals EFFECT OF DEUTERIUM WATER ON BLOOD VALUES AND DIGESTIBILITY OF NUTRIENTS OF RHESUS MACAQUE

2020 ◽  
Vol 384 (2) ◽  
pp. 22-28 ◽  
Author(s):  
N. V. Gaponov ◽  
S. V. Svistunov ◽  
N. N. Bondarenko ◽  
I. A. Romanenko
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
The Body ◽  
Control Group ◽  
Deuterium Content ◽  
Animal Blood ◽  
Constant Weight ◽  
Effect Of Water

The use of water with a modified isotopic composition in the diet of humans and animals, for example, with a reduced deuterium content, affects the rate of biological processes. In this regard, the targeted formation of the isotopic D / H gradient in the body can be used to increase its adaptive properties to external influences. Primates have a high anatomical and physiological similarity with humans, therefore, are an indispensable model for reproducing various pathological and toxic conditions in humans. The aim of the study was to study the effect of water with a low deuterium content on the hematological and biochemical blood parameters of rhesus monkeys and on the digestibility of nutrients of the diet. In the control group, primates consumed water with a natural regional content of deuterium equal to 150 mg / l, and in the experimental group - with reduced to 50 mg/l. The duration of the experiment was 35 days. The material for the study was venous blood, serum and feces of primates. Blood was drawn from monkeys before the use of deuterium depleted water and after the completion of the experiment. All blood samples (2.5-3.0 ml) were taken from the cubital or femoral veins of animals on an empty stomach and stabilized with a heparin solution. Laboratory studies of animal blood were performed on an automatic hematological analyzer (Beckman Coulter, USA) of the CoulterAcT 5diffCP brand. In order to study the effect of water with a reduced deuterium content on hematological parameters, we determined the number of red blood cells, white blood cells, platelets, hemoglobin concentration, hematocrit, the average volume of red blood cells, red blood cell anisocytosis, and erythrocyte sedimentation rate (ESR). The metabolism trial was divided into two periods. Preliminary - to exclude the influence of previous feeding and accustoming primates to cage conditions of keeping. Experimental - conducting a thorough accounting of consumed feed, excreted excrement. Feces were collected daily at the same time (morning and evening), weighed and ground in a mortar. At each collection, 50% of the homogenized mass was taken for analysis. The collected portions were stored in the refrigerator. After the end of the experimental (accounting) period in the collected feces, the initial moisture was determined by drying at 60-70 ℃ to constant weight. The resulting air-dry mass was thoroughly ground and analyzed. It has been found that the introduction of water with a reduced deuterium content into the diet of primates has a positive effect on feed conversion rate and blood biochemical parameters.

Reduced Arteriosclerotic Plaque Thrombus Formation

1981 ◽  
Author(s):  
M Dujovny ◽  
N Kossovsky ◽  
J-M Loubeau ◽  
R Segal ◽  
D Nelson
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Thrombus Formation ◽  
White Blood Cells ◽  
Control Group ◽  
X Rays ◽  
Aspirin 650Mg ◽  
Calcium Deposits ◽  
Pre Treatment ◽  
Cell Fragments

Sixty surgical patients with cerebrovascular accidents and angiographically diagnosed carotid plaques were divided into two groups: .1) pre-treatment with aspirin (650mg/day) and dipyridamol (25mg/tid) (42 patients); 2) no treatments (18 patients). The control group consisted of 10 autopsy of patients dying for unrelated cause.SEM examination of the endarterectomy plaque revealed intact red blood cells in 12 of 42 pre-treated and 18 of 18 untreated specimens; degenerating red blood cells and cell fragments (24/42, 18/18); fibrin (24/42, 18/18) and white blood cells (15/42, 18/18). These were frequently enmeshed in large surface thrombi. Cholesterol crystals (6 μm) were matted on the plaque surface (12/42, 15/18), and spherical globules averaging 3 to 10 μm sporadically blanketed the surface (6/42, 6/18). Plaque from the asymptomatic controls was smaller and less thrombotic. Energy dispersive analysis x-rays (EDAX) of the inner surface of all 70 specimens (50 years old +) revealed surface calcium deposits and nodules in 18 of the surgical cases (12/42, 6/18), and diffuse calcium deposits in 6 controls.Treatment with aspirin and dipyridamol significantly reduces plaque thrombosis.

Superoxide Dismutase (Erythrocuprein) and Free Radicals in Clinical Chemistry

1976 ◽  
Vol 13 (1-6) ◽  
pp. 393-398 ◽  
Author(s):  
J. M. C. Gutteridge
Keyword(s):  
Superoxide Dismutase ◽  
Free Radicals ◽  
Free Radical ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Clinical Chemistry ◽  
White Blood Cells ◽  
Superoxide Anions ◽  
Free Radical Chemistry ◽  
Cytochrome P 450

Erythrocuprein (superoxide dismutase) has recently been shown to have an enzymic function towards superoxide anions. The discovery of superoxide dismutase, its mode of action, and estimation are reviewed along with a brief introduction to oxygen activation and free-radical chemistry. The formation, activity, and destruction of oxygen free radicals in white blood cells, red blood cells, and subcellular particles are discussed, ( a) The production of superoxide anions by white cells during phagocytosis is thought to be advantageous for the overall bactericidal event. ( b) Normal red blood cells generate low levels of superoxide anions. Increased levels of free-radical production could play a significant role in accelerating cell ageing (haemolysis), ( c) Subcellular particles produce superoxide anions. These as well as organic peroxides have been implicated in drug hydroxylation reactions involving cytochrome P-450.

scholarly journals The Significance of Spermidine and Spermine in Association with Atherosclerosis in Sera of Iraqi Patients

2018 ◽  
Vol 11 (3) ◽  
pp. 1389-1396 ◽  
Author(s):  
Suhad A.Ibrahim ◽  
Jwan A. Zainulabdeen ◽  
Hameed M. Jasim
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
White Blood Cells ◽  
Coronary Intervention ◽  
Serum Levels ◽  
Hplc Method ◽  
Control Group ◽  
Percutaneous Coronary ◽  
Patient Groups ◽  
Positive Correlations

This study aims to determine the relationship between spermidine (SPD) and spermine (SPM) with the incidence of atherosclerosis in population of Iraqi patients in comparison with controls. A total of 128 atherosclerosis patients (64 male and 64 female) were included in the present study (aged 45-70 years). They were categorized into two groups: a group that underwent percutaneous coronary intervention catheterization (PCI) and a group that underwent diagnostic catheterization (DIG). 64 age matched, apparently healthy individuals (showing no symptoms of heart disease) of both men and women were also included in the control group (C). Sera were used as samples of the present study; the main measurements included SPD and SPM levels using the HPLC method. Results showed that serum levels of SPD were 631.53 ± 35.637, and 540.49 ± 44.564µg/ml in PCI and DIG patients, respectively. These levels showed significant increases (p˂0.001) when compared to their levels (29.162 ± 6.151µg/ml) in sera of healthy controls. On the other hand, the SPM levels were (159.98 ± 15.981, and 169.85 ±14.606 µg/ml) in PCI and DIG patients, respectively, which also show significant increases (p˂0.001) when compared with the SPM levels (24.26 ± 4.613 µg/ml) in the control group. Moreover, the changes in SPD and SPM levels differed significantly (p˂0.001, and p˂0.05, respectively) according to gender in patient groups. Moreover, it was found that there were positive correlations between sera levels of SPD and SPM in atherosclerosis patients with the levels of white blood cells (WBC) (r=0.58, p˂0.05, r=0.55, p˂0.05), neutrophils (r=0.54, p˂0.05, r=0.51, p˂0.05), lymphocytes (r=0.46, p˂0.05, r=0.48, p˂0.05) and red blood cells (RBC) (r=0.43, p˂0.05, r=0.60, p˂0.01). These results demonstrated that SPD and SPM levels were altered in sera of atherosclerosis patients when compared with the control group. In addition, the data indicated that these patients’ gender played a role in their levels. Generally, correlations were observed among the SPD and SPM levels, the white blood cell differential, and the red blood cells in the sera of atherosclerosis patients.

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