scholarly journals Serum protein profiles and C-reactive protein in natural canine filariasis

2021 ◽  
Vol 14 (4) ◽  
pp. 860-864
Author(s):  
Sariya Asawakarn ◽  
Sujin Sirisawadi ◽  
Nanthida Kunnasut ◽  
Patchana Kamkong ◽  
Piyanan Taweethavonsawat
Keyword(s):  
Serum Protein ◽  
Total Protein ◽  
Dirofilaria Immitis ◽  
Reference Range ◽  
Buffy Coat ◽  
Detection Methods ◽  
Protein Profiles ◽  
C Reactive Protein ◽  
Normal Reference Range ◽  
Reactive Protein

Background and Aim: Canine filariasis is caused by several species of filarial worms. The pathophysiological response to infection is mainly due to the filaria lifecycle. Laboratory detection methods to assess the pathological alterations characteristic of filariasis are needed urgently. Serum protein profiles and C-reactive protein (CRP) levels are used widely to diagnose several animal diseases. This study aimed to determine the serum protein profiles and CRP levels in dogs infected with Dirofilaria immitis or Brugia pahangi or both parasites. Materials and Methods: Blood samples were collected from 980 dogs presenting at animal hospitals and veterinary clinics in Bangkok and its vicinity. The presence of microfilaria in samples was determined using a buffy coat smear and staining with Wright–Giemsa. The sheathed and unsheathed microfilaria species were identified by acid phosphatase staining. Forty positive samples were tested. The serum protein profiles were identified by agarose gel electrophoresis. The CRP concentration was measured using a fluorescent immunoassay. Results: Albumin levels and albumin-to-globulin ratios were significantly lower, and total protein, β2 globulin, and γ globulin levels were significantly elevated in dogs infected with D. immitis and B. pahangi compared with reference values in normal dogs. The average CRP concentrations in dogs infected with D. immitis or B. pahangi were 69.9 and 12.9 mg/L, respectively. Conclusion: The total protein and γ globulin levels increased in canine filariasis compared with the normal reference range. The CRP concentration in dogs infected with D. immitis was extremely high, whereas that in dog infected with B. pahangi was normal.

scholarly journals Characterization of serum protein electrophoresis patterns and C-reactive protein in canine tick-borne diseases

2021 ◽  
pp. 2150-2154
Author(s):  
Sariya Asawakarn ◽  
Piyanan Taweethavonsawat
Keyword(s):  
Health Status ◽  
Serum Protein ◽  
Buffy Coat ◽  
Protein Electrophoresis ◽  
Blood Parasite ◽  
Serum Protein Electrophoresis ◽  
Hepatozoon Canis ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Worldwide Distribution

Background and Aim: Canine tick-borne diseases are important diseases with a worldwide distribution. In Thailand, the most important canine tick-borne diseases are ehrlichiosis, babesiosis, and hepatozoonosis. This study aimed to determine the serum protein electrophoresis patterns (SPEPs) and C-reactive protein (CRP) levels associated with Ehrlichia canis, Babesia canis, or Hepatozoon canis single infections. Materials and Methods: A total of 650 canine blood samples were collected from animal hospitals and clinics in Bangkok and its vicinity to examine health status and blood parasite infection. Suspected blood parasite infections were examined by buffy coat thin blood smear and confirmed by polymerase chain reaction. Normal dog and positive E. canis, B. canis, and H. canis single infections and serum protein profiles were determined by agarose gel electrophoresis. CRP concentration was measured by fluorescent immunoassay. Results: In dogs infected with E. canis, B. canis, and H. canis single infections, albumin levels and A/G ratios significantly decreased, whereas β2-globulin levels increased (p<0.05). The γ-globulin level significantly increased in E. canis and H. canis infections (p<0.05). A monoclonal gammopathy pattern was observed in E. canis and B. canis single infections, whereas β-γ bridging patterns and increased β- and γ-globulin fractions were found in H. canis single infections. The CRP level increased in dogs with blood parasite single infections and may be related to the pathogenesis of the infection. Conclusion: SPEPs and CRP levels can be used to monitor health status and blood parasite problems in infected dogs.

Development of ELISA on microplate for serum C-reactive protein and establishment of age-dependent normal reference range

2002 ◽  
Vol 322 (1-2) ◽  
pp. 163-168 ◽  
Author(s):  
Tsu-Lan Wu ◽  
Kuo-Chien Tsao ◽  
Christine P.-Y Chang ◽  
Chia-Ni Li ◽  
Chien-Feng Sun ◽  
...  
Keyword(s):  
Reference Range ◽  
C Reactive Protein ◽  
Normal Reference Range ◽  
Reactive Protein ◽  
Normal Reference ◽  
Age Dependent

scholarly journals Septicemia detection by blood buffy coat smear in primary health care centers

2012 ◽  
Vol 7 (1) ◽  
pp. 19-23
Author(s):  
BK Jha ◽  
YI Singh ◽  
S Mahadevmurthy ◽  
NK Chaudhary
Keyword(s):  
Health Care ◽  
Blood Culture ◽  
Early Diagnosis ◽  
Hospital Setting ◽  
Buffy Coat ◽  
C Reactive Protein ◽  
Klebsiella Species ◽  
Reactive Protein ◽  
Primary Health Care Centers ◽  
Health Care Centers

Early diagnosis of childhood septicemia can be done by simple tests like C-reactive protein (CRP) and anticoagulant added blood centrifuged buffy coat smear (BBCS) examination, where there is no well equipped hospital setting for blood culture and identification facility in remote health care centers. This study was conducted between 1st Jan. 2007 to 27th Dec. 2007 in College of Medical Sciences, Bharatpur, Nepal. In this study we have selected 150 suspected cases of childhood septicemia for screening CRP by kit method and BBCS by two slide techniques. This kit is supplied by Span Diagnostic Pvt. Ltd. (Surat, India). Out of 150 cases of childhood septicemia of age group 0-14 years, 83 had positive C- reactive protein (CRP >6ì g/ml), 70 were positive for BBCS and blood culture was positive only in 83 cases, where predominant organism being Klebsiella species followed by Staphylococcus species. CRP test showed 100.0% sensitivity and 87.30% specificity, where BBCS showed 76.5% sensitivity and 91.2% specificity. Blood culture reports are available only after 48-72 hours and this facility is available only in well equipped centers but CRP and BBCS are easy and cheap procedure to perform even in remote areas for early diagnosis of childhood septicemia. DOI: http://dx.doi.org/10.3126/jcmsn.v7i1.5968 JCMSN 2011; 7(1): 19-23

scholarly journals Detection of C-Reactive Protein Using Histag-HRP Functionalized Nanoconjugate with Signal Amplified Immunoassay

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1240
Author(s):  
Mohd Farhan Siddiqui ◽  
Zeeshan Ahmad Khan ◽  
Seungkyung Park
Keyword(s):  
Detection Methods ◽  
Enzyme Linked Immunosorbent Assay ◽  
C Reactive Protein ◽  
Serum Samples ◽  
Reactive Protein ◽  
Elisa Format ◽  
Early Biomarker ◽  
Cost Efficient ◽  
Detection Of Biomarkers ◽  
Gold Nanoprobe

Ultrasensitive detection of biomarkers is highly significant for disease prognosis and public health treatment. Despite wide acceptance in routine laboratory tests, the conventional enzyme-linked immunosorbent assay (ELISA) has been of limited use for early biomarker detection due to insufficient sensitivity and multiple long incubation time. Several nanoprobes have been introduced to circumvent the limitation, however, rapid, simple, and chemical-free nanoprobe synthesis and sensitive detection methods, particularly for ELISA, are still lacking. In this study, we have synthesized a gold nanoprobe, conjugated with multiple 6X-histidine (6X-his) peptide and nickel-horseradish peroxidase (Ni2+-HRP), for enhancing the colorimetric signal in ELISA. The developed nanoprobe has been tested for the detection of immunologically significant C-reactive protein (CRP) in ELISA format. The performance of designed probe is validated by testing standard and serum samples, and the detection limit of 32.0 pg/mL with R2 = 0.98 is confirmed. Furthermore, a comparative analysis of the developed nanoprobe was performed with ELISA developed on conventional guidelines, the proposed immunoassay showed an increase of 12-fold sensitivity for detecting CRP due to the high loading of 6Xhis peptide and binding of multiple Ni2+-HRP on a gold nanoparticle. Additionally, the proposed assay provides a simple, fast, and cost-efficient (not requiring multiple antibodies) detection of CRP with easy nanoprobe synthesis. Moreover, the developed Histag-HRP functionalized nanoconjugate immunoassay is flexible and can be applied to other biomarkers efficiently by using disease specific antibody.

Strenuous exercise: analogous to the acute-phase response?

1991 ◽  
Vol 81 (5) ◽  
pp. 677-683 ◽  
Author(s):  
Lindsay M. Weight ◽  
Donald Alexander ◽  
Peter Jacobs
Keyword(s):  
Acute Phase ◽  
Total Protein ◽  
Protein Level ◽  
Acute Phase Response ◽  
Interleukin 1 ◽  
Phase Response ◽  
Strenuous Exercise ◽  
Iron Level ◽  
C Reactive Protein ◽  
Reactive Protein

1. It has been suggested that the physiological consequences of strenuous exercise are analogous to those of the acute-phase response. 2. In 70 male and 20 female competitive distance runners, a marked, but transient, neutrophil leucocytosis occurred immediately after these athletes completed a standard (42 km) marathon race. Concomitant significant increases were noted in the plasma cortisol levels, creatine kinase activity, C-reactive protein level, total protein level and albumin level (P <0.01). 3. The plasma fibrinogen, C-reactive protein and total protein concentrations were markedly increased both 24 h and 48 h after exercise (P <0.01). The serum haptoglobin level was significantly decreased after exercise (P <0.01), and increased 48 h later (P <0.05). There was no change in the serum iron level, total iron-binding capacity, per cent saturation of transferrin and serum ferritin level. 4. A significant increase in interleukin-1-type activity was demonstrated immediately and 24 h after exercise (P <0.01). 5. It is concluded that the metabolic sequelae of sustained exercise are similar, but not analogous, to the acute-phase response, and interleukin-1 probably plays a significant role in linking the haematological and immunological changes observed after sustained strenuous exercise.

scholarly journals Triage Strategies Based on C-Reactive Protein Levels and SARS-CoV-2 Tests among Individuals Referred with Suspected COVID-19: A Prospective Cohort Study

2021 ◽  
Vol 11 (1) ◽  
pp. 201
Author(s):  
Erika Olivia Boyesen ◽  
Ida Maria Balsby ◽  
Marius Henriksen ◽  
Robin Christensen ◽  
Jens Henning Rasmussen ◽  
...  
Keyword(s):  
Prognostic Value ◽  
Total Population ◽  
Reference Range ◽  
Hospitalized Patients ◽  
Test Results ◽  
C Reactive Protein ◽  
Protein Levels ◽  
Reactive Protein ◽  
Oropharyngeal Swab ◽  
Pcr Test

C-reactive protein (CRP) has prognostic value in hospitalized patients with COVID-19; the importance of CRP in pre-hospitalized patients remains to be tested. Methods: Individuals with symptoms of COVID-19 had a SARS-CoV-2 PCR oropharyngeal swab test, and a measurement of CRP was performed at baseline, with an upper reference range of 10 mg/L. After 28 days, information about possible admissions, oxygen treatments, transfers to the ICU, or deaths was obtained from the patient files. Using logistic regression, the prognostic value of the CRP and SARS-CoV-2 test results was evaluated. Results: Among the 1006 patients included, the SARS-CoV-2 PCR test was positive in 59, and the CRP level was elevated (>10 mg/L) in 131. In total, 59 patients were hospitalized, only 3 of whom were SARS-CoV-2 positive, with elevated CRP (n = 2) and normal CRP (n = 1). The probability of being hospitalized with elevated CRP was 4.21 (95%CI 2.38–7.43, p < 0.0001), while the probability of being hospitalized with SARS-CoV-2 positivity alone was 0.85 (95%CI 0.26–2.81, p = 0.79). Conclusions: CRP is not a reliable predictor for the course of SARS-CoV-2 infection in pre-hospitalized patients. CRP, while not a SARS-CoV-2 positive test, had prognostic value in the total population of patients presenting with COVID-19-related symptoms.

scholarly journals Neutrophil myeloperoxidase index in pediatric acute appendicitis

2019 ◽  
Vol 76 (9) ◽  
pp. 898-905 ◽  
Author(s):  
Nikola Stankovic ◽  
Dragan Djordjevic ◽  
Goran Rondovic ◽  
Zoran Kostic ◽  
Snjezana Zeba ◽  
...  
Keyword(s):  
Acute Appendicitis ◽  
Reference Range ◽  
Delayed Diagnosis ◽  
Healthy Children ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Significant Difference ◽  
Positive Correlations ◽  
Prospective Investigation

Background/Aim. Diagnosis of acute appendicitis (AA) remains the most common dilemma of pediatric surgical team. Our aim was to determine whether the neutrophil myeloperoxidase index (MPXI), in combination with other laboratory and clinical parameters, can be useful in diagnosis and follow-up of AA in children. Methods. A prospective investigation of MPXI values in 117 consecutive patients, planned for the surgical intervention due to AA, was performed. The patients were stratified into three groups according to the intraoperative finding: the normal/early, uncomplicated and complicated AA. Laboratory analyses were done preoperativly, on the 1st and on the 3rd postoperative days. Results. The statistically significant difference of MPXI values between the uncomplicated and complicated appendicitis before surgery and the positive correlations between the MPXI and C-reactive protein, as well as interleukin- 6, before surgery were found. Postoperatively, in the group of uncomplicated, as well as complicated AA, a significant decrease of MPXI was recorded. Conclusion. The MPXI may be used as an informative biomarker in the follow- up of AA in children. A wide reference range for the MPXI and individual differences in the values of MPXI in the healthy children, generate difficulties for its use for the initial diagnosis of acute appendicitis. Usefulness of MPXI determination decreases with a delayed diagnosis.

scholarly journals PSV-35 Correlation of acute-phase blood proteins level and quality and cryostability of sperm in horses

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 337-338
Author(s):  
Mikhail Atroshchenko ◽  
Mariya Engalycheva ◽  
Anna Kudlaeva ◽  
Elena Borodkina ◽  
Mariya Fomina ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Acute Phase ◽  
Total Protein ◽  
Development Program ◽  
The Body ◽  
Blood Proteins ◽  
C Reactive Protein ◽  
Russian Science ◽  
Reactive Protein ◽  
Hypothermic Storage

Abstract The aim of this work was to study the relationship between acute phase indicators of a biochemical blood test with the quality and cryostability sperm in stallions. Semen from 59 stallions (aged from 5 to 15 years) were collected during the breeding season (February–May). Blood plasma samples were studied using a Vegasys automatic biochemical analyzer (Analyzer Medical System, Italy). The following parameters were determined in blood plasma: total protein, level of albumin, globulin, albumin/globulin ratio, C-reactive protein, fibrinogen. Each sperm sample was evaluated by volume, concentration, total sperm, as well as progressive motility and survival of sperm in chilled and thawed sperm during hypothermic storage at +40С. The normality of the distribution of quantitative traits was determined using the Shapiro-Wilk test. For each of the indicators, the Spearman coefficient was calculated. Significance of differences was assessed using the Mann-Whitney test, the results were statistically significant at P ≤0.05. The concentration of biochemical parameters in blood serum averaged: total protein - 67.4 ± 0.7 g/l, albumin - 39.4±0.6 g/l, globulins - 28.4±0.8 g/l, C reactive protein - 0.3±0.1 mg/l, fibrinogen - 3.1±0.1 g/l. A significant negative correlation between the plasma fibrinogen and sperm survival during hypothermic storage (at +4ºС) of chilled (r=0.48; P &lt; 0.001) sperm and progressive sperm motility after thawing (r =-0.29; &lt; 0.05) was found. We suggest that an increase in the level of fibrinogen in the blood may indicate the presence of latent inflammatory, immuno-mediated processes in the body, including those affecting the quality and cryostability of sperm in stallions. Authors acknowledge financial support from Russian Science Foundation, Grant No: 20-16-00101, development program of Bioresource collections “Cryobank of genetic recourses the All-Russian Research Institute for Horse Breeding.”

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