scholarly journals Theoretical Molecular Properties Prediction, Docking and Antimicrobial Studies on Anisidine-Isatin Schiff Bases

2021 ◽  
Vol 33 (12) ◽  
pp. 3025-3030
Author(s):  
G.K. Ayyadurai ◽  
R. Jayaprakash ◽  
S. Rathika
Keyword(s):  
Staphylococcus Aureus ◽  
Schiff Bases ◽  
Staphylococcus Epidermidis ◽  
Biological Activities ◽  
Position Effect ◽  
Group Position ◽  
Antimicrobial Studies ◽  
Main Protease ◽  
Lactam Ring ◽  
Molecular Properties Prediction

The continuous intake of a specific antibiotic for diseases is continuously reducing the immunity of the human in course of time, which includes Covid-19 treatment. Recent research on Schiff bases shows the promising biological activities and good antibacterial results. In this study, three Schiff bases with lactam ring using isatin and three different anisidines in presence of acetic acid were synthesized and characterized. Drug likeness was examined using Molsoft and docking against the target proteins such as 5J6R, 3L9L, 5HVY, covid main protease and 3ZBO proteins for drug suitability. The experimental antibacterial activity against Gram-positive strains like Staphylococcus aureus, Bacillus subtilis and Staphylococcus epidermidis. Among the synthesized compounds, three Schiff bases ortho and meta substituted compounds exhibited good results when compared to para compound, where the methoxyl group position effect was observed.

Formulation of Nanoemulgel from Extracts of Musa acuminata: In-Vitro Kinetics and Antimicrobial Studies

2021 ◽  
pp. 1-8
Author(s):  
Gaanapriya Veeramani ◽  
Sivakumar Vaiyazhipalayam Murugaiyan ◽  
Thirumarimurugan Marimuthu
Keyword(s):  
Staphylococcus Epidermidis ◽  
Leaf Extract ◽  
Biological Activities ◽  
Musa Acuminata ◽  
Alpha Tocopherol ◽  
Malassezia Furfur ◽  
Antimicrobial Studies ◽  
Ph Range ◽  
In Vitro Kinetics

The study was intended to formulate nanoemulgel from the leaf extract of Musa acuminata. Ethanol and Chloroform were used as solvents in the ratio of 1:8 (%w/v). DL- alpha-Tocopherol, characterized by GCMS, was identified to be the major component with potential biological activities. Based on a pseudo ternary plot, the 1:1 Smix (Surfactant: Co- Surfactant) ratio was optimized as it posed maximum regions of emulsion. The prepared nanoemulgel was evaluated for physical appearance, pH, spreadability, and swelling index. The appearance was pale yellowish-white, translucent within a pH range of 5-5.8. Antimicrobial studies were performed against dandruff-causing microbes (Staphylococcus epidermidis and Malassezia furfur). Invitro studies were carried out for optimized formulations of EG2, EG4, CG2, and CG3. The drug release of 94.28% after 12 h with Higuchi plot of R2 value as 0.99 was observed for EG2. The kinetically optimized formulation, EG2 was found to have good spreadability of 12.2 (g cm) s−1 and a swelling index of 64%.

scholarly journals Effects of Ulva sp. Extracts on the Growth, Biofilm Production, and Virulence of Skin Bacteria Microbiota: Staphylococcus aureus, Staphylococcus epidermidis, and Cutibacterium acnes Strains

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4763
Author(s):  
Mathilde Fournière ◽  
Gilles Bedoux ◽  
Djouhar Souak ◽  
Nathalie Bourgougnon ◽  
Marc G. J. Feuilloley ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Biological Activities ◽  
Opportunistic Pathogen ◽  
Bacterial Biofilm ◽  
Skin Bacteria ◽  
Assisted Extraction ◽  
Cutibacterium Acnes ◽  
First Time

Ulva sp. is known to be a source of bioactive compounds such as ulvans, but to date, their biological activity on skin commensal and/or opportunistic pathogen bacteria has not been re­ported. In this study, the effects of poly- and oligosaccharide fractions produced by enzyme-assisted extraction and depolymerization were investigated, for the first time in vitro, on cutaneous bacteria: Staphylococcus aureus, Staphylococcus epidermidis, and Cutibacterium acnes. At 1000 μg/mL, poly- and oligosaccharide fractions did not affect the growth of the bacteria regarding their generation time. Polysaccharide Ulva sp. fractions at 1000 μg/mL did not alter the bacterial biofilm formation, while oligosaccharide fractions modified S. epidermidis and C. acnes biofilm structures. None of the fractions at 1000 μg/mL significantly modified the cytotoxic potential of S. epidermidis and S. aureus towards keratinocytes. However, poly- and oligosaccharide fractions at 1000 μg/mL induced a decrease in the inflammatory potential of both acneic and non-acneic C. acnes strains on keratinocytes of up to 39.8%; the strongest and most significant effect occurred when the bacteria were grown in the presence of polysaccharide fractions. Our research shows that poly- and oligosaccharide Ulva sp. fractions present notable biological activities on cutaneous bacteria, especially towards C. acnes acneic and non-acneic strains, which supports their potential use for dermo-cosmetic applications.

Untersuchungen zur aeroben bakteriellen Besiedlung der Vaginalschleimhaut klinisch gesunder Kätzinnen

2004 ◽  
Vol 32 (02) ◽  
pp. 88-91
Author(s):  
Susanne Kloß ◽  
A. Wehrend ◽  
Astrid König ◽  
H. Bostedt
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Pasteurella Multocida ◽  
E Coli ◽  
Klinische Relevanz ◽  
Staphylococcus Intermedius

Zusammenfassung: Gegenstand und Ziel: Im Gegensatz zur Hündin liegen bei der Katze bisher wenige Studien über die genitale Keimflora geschlechtsgesunder Tiere vor. Ziel der Untersuchung war daher, physiologische Daten über die aerobe Vaginalflora bei dieser Spezies zu gewinnen. Material und Methoden: Für die vorliegende Studie standen 26 gesunde, anöstrische Katzen zur Verfügung, die zu einer Ovariohysterektomie vorgestellt wurden. Nach einer klinischen Untersuchung wurden von allen Probanden unter sterilen Bedingungen Vaginaltupfer entnommen. Ergebnisse: In allen Proben konnte ein Bakterienwachstum mit durchschnittlich zwei verschiedenen Bakterienspezies nachgewiesen werden. Die Gesamtkeimgehalte wurden bei 50% der Vaginaltupferproben als gering-, bei 15% als mittel- und bei 35% als hochgradig beurteilt. Vorherrschend waren Mischkulturen aus zwei bis vier verschiedenen Keimarten. Monokulturen wurden aus 38% der Tupferproben isoliert. Am häufigsten gelang der Nachweis von E. coli variatio haemolytica (E. coli var. haem.) (58%) und Staphylococcus epidermidis (42%). Als weitere Spezies wurden E. coli, α-, β-hämolysierende Streptokokken, anhämolysierende Streptokokken, aerobe Bazillen, Staphylococcus aureus, Staphylococcus intermedius, Pasteurella multocida sowie Klebsiellen isoliert. Auffällig ist die hohe Nachweisrate von E. coli var. haem. mit 35% in Mischkulturen und 23% in Reinkultur. Schlussfolgerungen: Die physiologische Mikroflora der felinen Vaginalschleimhaut differiert deutlich von der der anöstrischen Hündin. Besonders die Dominanz von E. coli var. haem. in 38% der Mischkulturen und 23% der Monokulturen bei der Katze ist hervorzuheben. Klinische Relevanz: Die vorliegenden Ergebnisse geben eine erste Grundlage für die Interpretation mikrobiologischer Befunde feliner Vaginaltupfer.

scholarly journals The bactericidal effect of vancomycin is not altered by tranexamic acid, adrenalin, dexamethasone, or lidocaine in vitro

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Christiane Schwerdt ◽  
Eric Röhner ◽  
Sabrina Böhle ◽  
Benjamin Jacob ◽  
Georg Matziolis
Keyword(s):  
Staphylococcus Aureus ◽  
Tranexamic Acid ◽  
Total Joint Arthroplasty ◽  
Staphylococcus Epidermidis ◽  
Joint Infection ◽  
Bactericidal Effect ◽  
Joint Arthroplasty ◽  
Zone Of Inhibition ◽  
Agar Diffusion Test

AbstractOne of the most challenging complications of total knee arthroplasty (TKA) is periprosthetic joint infection (PJI). There is growing evidence of a good anti-infective effect of intrawound vancomycin powder in total joint arthroplasty. At the same time, various different locally applied substances have become popular in total joint arthroplasty. The objective of this study was therefore to investigate a possible inhibition of the bactericidal effect of vancomycin by tranexamic acid, adrenalin, lidocaine, or dexamethasone. The bactericidal effect of vancomycin was quantified using the established method of the agar diffusion test. The plates were incubated with Staphylococcus aureus or Staphylococcus epidermidis and four wells were stamped out. The wells were filled with vancomycin alone, the tested substance alone or a mixture of the two. The fourth well remained empty as a control. The plates were incubated overnight at 37 °C and the zone of inhibition in each field was measured on the next day. All tests were run three times for each pathogen and mean values and standard deviations of the measurements were calculated. Differences between the substances were tested using the t-test at a level of significance of 0.05. The bacterial growth was homogeneous on all plates. The baseline value for the zone of inhibition of vancomycin was on average 6.2 ± 0.4 mm for Staphylococcus aureus and 12 ± 0.3 mm for Staphylococcus epidermidis. In all other substances, no inhibition was detected around the well. The combination of vancomycin and each other substance did not show any different result compared to vancomycin alone. The bactericidal effect of vancomycin on staphylococci is not altered by tranexamic acid, adrenalin, dexamethasone, or lidocaine in vitro.

scholarly journals A Novel Antimicrobial Phenanthrene Alkaloid from Bryopyllum pinnatum

2011 ◽  
Vol 8 (3) ◽  
pp. 1456-1461 ◽  
Author(s):  
Donatus Ebere Okwu ◽  
Fred Uchenna Nnamdi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Aspergillus Niger ◽  
Ethanolic Extract ◽  
Klebsiella Pneumonia ◽  
Antimicrobial Studies ◽  
Prevention And Treatment ◽  
Kalanchoe Pinnata ◽  
Isolated Compound

Phenenthrene alkaloid identified as 1-ethanamino 7 hex-1-yne-5I-one phenanthrene was isolated from the ethanolic extract of the leaves ofBryophyllum pinnatum(syn. B. calcinum kalanchoe pinnata) a versatile Nigeria medicinal plant. The structure was elucidated using NMR, IR, UV and MS spectral data. Antimicrobial studies showed that the isolated compound successfully inhibitedPsuedomonas aeruginosa, Klebsiella pneumonia, Staphylococcus aureus, Escherichia coli, Candida albicansandAspergillus niger. This result authenticates the use ofbryophyllum pinantumin phytomedicine for disease prevention and treatment of infections.

scholarly journals Pheromone Cross-Inhibition betweenStaphylococcus aureus and Staphylococcus epidermidis

2001 ◽  
Vol 69 (3) ◽  
pp. 1957-1960 ◽  
Author(s):  
Michael Otto ◽  
Hartmut Echner ◽  
Wolfgang Voelter ◽  
Friedrich Götz
Keyword(s):  
Staphylococcus Aureus ◽  
Quorum Sensing ◽  
Cross Talk ◽  
Medical Devices ◽  
Staphylococcus Epidermidis ◽  
Selective Pressure ◽  
Close Relation ◽  
Cross Inhibition

ABSTRACT Cross-inhibition by quorum-sensing pheromones betweenStaphylococcus aureus and Staphylococcus epidermidis was investigated using all known S. aureus agr pheromone subgroups. All S. aureus subgroups were sensitive towards the S. epidermidis pheromone, with the exception of the recently identified subgroup 4. The subgroup 4 pheromone was also the only S. aureus pheromone able to inhibit the S. epidermidis agr response. The close relation of subgroup 4 to subgroup 1 suggests that subgroup 4 might have evolved from subgroup 1 by mutation under the selective pressure of competition with S. epidermidis. The competition between S. aureus and S. epidermidis by means of quorum-sensing cross talk seems to be generally in favor of S. epidermidis, which might explain the predominance of S. epidermidis on the skin and in infections on indwelling medical devices.

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