scholarly journals THE ANTIHYPOXIC ACTIVITY OF SIBERIAN FIR ESSENTIAL OIL FRACTIONS

2020 ◽  
pp. 273-280
Author(s):  
Dmitriy Konstantinovich Gulyayev ◽  
Ekaterina Igorevna Yakovleva ◽  
Petr Sergeevich Mashchenko ◽  
Sergey Yuryevich Solodnikov ◽  
Valentina Dmitrievna Belonogova
Keyword(s):  
Essential Oil ◽  
Reference Sample ◽  
Coniferous Forest ◽  
Component Composition ◽  
Raw Material ◽  
Antihypoxic Activity ◽  
Siberian Fir ◽  
Dark Coniferous Forest ◽  
Oil Fractions

The component composition and antihypoxic activity of Siberian fir (Abies sibirica) essential oil fractions were researched. The foliage of Siberian fir, collected on the territory of the Ilyinsky district of the Perm region in a dark coniferous forest was the raw material for obtaining the essential oil. Samples for the research were collected in December 2018 from trees 40–50 years old. The essential oil was obtained with the Clevenger apparatus. Determination of the component composition of Siberian fir essential oil fractions was made by using chromatography-mass-spectrometry. Experiments for determination of antihypoxic activity of Siberian fir essential oil fractions were made on a hypoxic hypoxia model with hypercapnia. According to the results of the research, it was found that the first fraction is more saturated with monoterpene compounds, but the proportion of sesquiterpenoids increases in following fractions. The main components of all fir oil fractions are: bornylacetate, limonene, ∆3-carene, karyophyllene. It was found that the fractions 1 and 2 have the highest antihypoxic activity in comparison with the reference sample. For further research, we offer fraction 1, because it contains the high level of bornylacetate, and the lowest content of ∆3-carene, which inhibits the ubiquinol cytochrome-C reductase complex, interrupting mitochondrial and cell respiration.

scholarly journals COMPONENT COMPOSITION OF ESSENTIAL OIL PRUNUS PERSICA VAR. NECTARINA GROWING IN UZBEKI-STAN

2020 ◽  
pp. 165-170
Author(s):  
Ra"no Botirovna Karabaeva ◽  
Alidzhan Aminovich Ibragimov ◽  
Otabek Mamadaliyevich Nazarov
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Prunus Persica ◽  
Component Composition ◽  
Raw Material ◽  
Oil Composition ◽  
Pale Yellow ◽  
Yellow Color ◽  
The Republic ◽  
Main Components

The article presents the results of determining the composition of essential oils of Prunus persica var. nectarina varieties "yellow nectarine" – "sariк luchchak (uzb.) ", growing in two regions of the Ferghana region of the Republic of Uzbekistan. The pale yellow essential oil was obtained by hydrodistillation from un-dried fresh leaves of plants. The oil composition was determined by chromatography-mass spectrometry on an Agilent 7890 AGC 6890 N gas chromotograph with a quadrupole mass spectrometer (Agilent 5975C inert MSD) as a detector on an HP-5 MS quartz capillary column. An essential oil of pale yellow color was isolated from the leaves of plants. In the composition of essential oils, 56 and 61 compounds were identified in the first and second samples, respectively, wich is 94.55 and 96.00% of the total components. The dominant components of the first saple are camphor bicyclic monoterpene ketones (24.21%), α-thujone (15.00%) and β-thujone (4.27%), aromatic aldehyde benzaldehyde (18.83%) and isobornoleol bicyclic monoterpene alcohol (6.17%). In the second sample, bicyclic monoterpenic ketones (camphor) (36.67%), α-thujone (21.81%) and β-thujone (7.06%) and bicyclic monoterpene alcohol isobornoleol (9.4%) predominate and monocyclic unsaturated monoterpene α-terpinene (2.18%). In both samples, (+)-2-bornanone (camphor) is predominant. The studied variety Prunus persica var. nectarina can serve as a raw material for the production of essential oil, the main components of which are camphor and isobornoleol.

scholarly journals До питань використання та ідентифікації неофіцинальної сировини – листя Acorus Calamus L.

2018 ◽  
Author(s):  
M. S. Yaremenko ◽  
T. M. Gontova ◽  
L. M. Sira
Keyword(s):  
Essential Oil ◽  
Component Composition ◽  
Raw Material ◽  
Acorus Calamus ◽  
Control Methods ◽  
Diagnostic Features ◽  
Documentation Quality ◽  
Quantitative Content ◽  
Medical Plant ◽  
Normative Documentation

Introduction. Acoruscalamus L. is a perspective medical plant for complex use in medicine.The aim of the study – comparison of the quantitative content of essential oil and its component composition in the rhizomes and leaves of calamus, establishing the prospects for using leaves, revealing the morphological and anatomical diagnostic features of leaves as a new raw material.Research Methods. The content and composition of the essential oil were studied using the CMS method, the microscopic characteristics of the raw material – generally accepted analytical methods.Results and Discussion. A comparative analysis of the quantitative content and qualitative composition of the essential oil components of the acoruscalamus were conducted. The established diagnostic morphological and anatomical signs of the leaves will be used in the development of normative documentation – quality control methods (QCM) for additional raw materials.Conclusion. The results of the studies indicate the prospect of further pharmacognostic study of the leaves of the calamus as an additional and accessible raw material.

COMPONENT COMPOSITION AND BIOLOGICAL ACTIVITY OF ESSENTIAL OIL OF LIGULARIA HETEROPHYLLA RUPR.

2020 ◽  
pp. 239-244
Author(s):  
Aigerim Makubayeva ◽  
Elena Petrovna Romanenko ◽  
Sergazy Mynzhasarovich Adekenov ◽  
Aleksey Vasil'yevich Tkachev
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Component Composition ◽  
Raw Material ◽  
Inflammatory Activity ◽  
Microwave Extraction ◽  
Mass Selective Detector ◽  
Light Yellow ◽  
Anti Inflammatory ◽  
First Time

The object of the study – the raw material of Ligularia heterophylla Rupr., collected in 2019 in the Almaty region of the Republic of Kazakhstan. The component compositions of essential oils from the aerial and underground parts of Ligularia heterophylla Rupr., obtained by methods of microwave extraction, as well as traditional hydrodistillation, were studied for the first time. Essential oils represented as mobile liquids from light yellow to green shade with characteristic odors. The component composition was determined by chromato-mass spectrometry on an Agilent 6890 gas chromatograph equipped with a MSD 5973 mass-selective detector. Essential oils obtained by the hydrodistillation method contain mainly monoterpenes, while sesquiterpenes predominate in the essential oils obtained by microwave extraction. The antimicrobial and anti-inflammatory activity of Ligularia heterophylla Rupr. essential oil samples was studied for the first time. The essential oil from the underground part (roots) of Ligularia heterophylla Rupr., obtained by microwave extraction method, at a dose of 25 mg/kg, has a pronounced anti-inflammatory activity, comparable to the drug «Diclofenac sodiumа» in the model of acute exudative reaction.

Determination of essential oil component composition of common sunflower marginal flowers

2018 ◽  
Vol 11 (5) ◽  
pp. 1971
Author(s):  
T.M. Gontova ◽  
O.O. Sokolova ◽  
A.G. Kotov ◽  
S.A. Kutsenko ◽  
V.V. Mashtaler
Keyword(s):  
Essential Oil ◽  
Component Composition

scholarly journals COMPARATIVE ANALYSIS OF ESSENTIAL OIL THE VEGETABLE RAW MATERIAL SCHISANDRA CHINENSIS: LEAVES, WOODY STEMS, RHIZOMES WITH ROOTS

2019 ◽  
pp. 173-180
Author(s):  
Юрий (Yuriy) Алексеевич (Alekseevich) Морозов (Morozov) ◽  
Ифрат (Ifrat) Назимович (Nazimovich) Зилфикаров (Zilfikarov) ◽  
Елизавета (Elizaveta) Владимировна (Vladimirovna) Морозова (Morozova) ◽  
Аслан (Aslan) Мурадалиевич (Muradalievich) Алиев (Aliev) ◽  
Тимур (Timur) Алгасанович (Algasanovich) Ибрагимов (Ibragimov)
Keyword(s):  
Comparative Analysis ◽  
Essential Oil ◽  
Essential Oils ◽  
Raw Materials ◽  
Component Composition ◽  
Raw Material ◽  
Gas Chromatography Mass Spectrometry ◽  
Schisandra Chinensis ◽  
Marker Substances ◽  
Vegetable Raw Materials

This paper presents the results of an experimental study of the component composition of essential oil samples obtained from various vegetable raw materials of Schisandra chinensis (Schisandra chinensis (Turcz.) Baill.): branches (woody stems), rhizomes with roots and leaves. The extraction of essential oils was carried out by distillation with water vapor followed by extraction from the distillate with ethoxyethane. The largest yield of essential oil in terms of completely dry raw material is registered from rhizomes with roots – 0.99%; the yield from leaves and branches was approximately the same: 0.51% and 0.52%, respectively. A comparative analysis of the component composition of the obtained essential oils was carried out using the gas chromatography / mass spectrometry method. In the essential oil of the leaves 29 detected compounds (identified by 21 connection; dominant components: Cyclohexylmethyl ether sulphurous acid, (+)-trans-nerolidol, δ-cadinene, Tridec-(2E)-EN-1-ol), woody stems 80 connections (identified 65 compounds; dominant components: ß-pinene, Camphene, Bornylacetate, (+)-trans-nerolidol, p-tsimen, Tridecan-2-it, δ-cadinene), rhizomes with roots 78 of the compounds (60 compounds identified; dominant components: δ-cadinene, Bornylacetate, β-pinene, Camphene, Nerolidol-(E), Tridecan-2-it, γ-cadinene, Borneol). For each sample of the studied essential oils selectivity and marker substances were established.

scholarly journals COMPONENT COMPOSITION OF ESSENTIAL OIL OF PINE BUDS (PINUS SYLVESTRIS L.), GROWING IN THE URBAN ENVIRONMENT OF TOMSK REGION

2019 ◽  
pp. 181-190
Author(s):  
Наталья (Natal'ya) Эдуардовна (Eduardovna) Коломиец (Kolomiets) ◽  
Наталья (Natal'ya) Юрьевна (Yur'evna) Абрамец (Abramets) ◽  
Руслан (Ruslan) Анатольевич (Anatol'evich) Бондарчук (Bondarchuk) ◽  
Валентина (Valentina) Германовна (Germanovna) Шириеторова (Shirietorova) ◽  
Жаргал (Zhargal) Александрович (Aleksandrovich) Тыхеев (Tyheev) ◽  
...  
Keyword(s):  
Essential Oil ◽  
Component Composition ◽  
Ion Source ◽  
Raw Material ◽  
Internal Diameter ◽  
Gas Liquid Chromatography ◽  
Gas Chromatograph ◽  
Column Temperature ◽  
Water Steam ◽  
Tomsk Region

The component composition of pine buds essential oil, growing in the area of active economic activity in Tomsk region, has been studied. Essential oil from pine buds obtained by the method of distillation with the water steam. The component composition of the essential oil was studied by gas-liquid-chromatography-mass spectrometry on the gas chromatograph Agilent 6890 with quadrupole mass spectrometer (HP MSD 5973N). Chromatography conditions: 30-meter long quartz column HP-5MS with internal diameter 0.25 mm, film thickness of 0.25 µm (copolymer 5% diphenyl 95% dimethylsiloxane), carrier gas – helium (constant flow 1 ml/min), column temperature – 50 °С (2 min isotherm), 50–200 °С (40 °C, min), 200–280 °С (20 °С/min), 280 °С (5 min isotherm), the temperature of the ion source 170 °С, the interface between the gas chromatograph and mass selective detector 280 °С. The energy of ionizing electrons is 70 eV. The volume of injected sample of 1 µl. The component composition was determined by comparing the times and linear retention indices, mass spectra values, the database of the chromatographic-mass spectrometric library of volatile substances of plant origin. About 163 individual components were found. It is established that with the increase of technogenic pollution, the amount of essential oil decreases, the component composition changes, which can serve as an indicator of the quality of raw materials, the state of the forest stand of pine and the environment as a whole. Data on the dominant content of 3-caren in the composition of essential oils of the pine buds, growing in the Tomsk region, suggests a limited use of this raw material and essential oil on its basis in medical practice.

scholarly journals Contents of biologically active substances in the leaves of Myrtus communis L. under the conditions of the south Crimea

2020 ◽  
Vol 192 (1) ◽  
pp. 60-68
Author(s):  
L. Logvinenko ◽  
E. Dunaevskaya
Keyword(s):  
Essential Oil ◽  
Raw Materials ◽  
Maximum Yield ◽  
Component Composition ◽  
Essential Elements ◽  
Biologically Active ◽  
Raw Material ◽  
Myrtus Communis ◽  
Myrtus Communis L

Abstract. The purpose of the study was to study the effect of various methods of cultivating the variety of common myrtle Yuzhnoberezhny on the change in the content of macro- and microelements in raw materials, to compare the component composition of the essential oil obtained in the long-term and overgrowth form of its cultivation. Myrtus communis L. is a representative of the Mediterranean plant community, which since ancient times has been used for medicinal purposes and as a spicy culture. In the Nikitsky Botanical Garden – the National Scientific Center of the Russian Academy of Sciences, a variety of common myrtle South Coast was created. The variety is recommended for industrial cultivation on the territory of the southern coast of Crimea. Methods. The mineral composition of the raw materials was determined by dry ashing of leaves cut in the phase of technological maturity. The content of seven essential elements was determined on the quantum 2MT atomic absorption spectrophotometer: potassium in the emission mode, calcium, magnesium, ferrum, manganese, cuprum and zinc in the absorption mode. The component composition was studied in samples of essential oil on an Agilent Technology 6890N chromatograph with a 5973N mass spectrograph detector. Results. The maximum yield of medicinal raw materials in these soil and climatic conditions was ensured sprout cultivation, in which the rate of shoot formation increased by 2.6 times, and the yield of leaf, which is the raw material for the essential oil and food industries, increased by 37 %. The method of cultivating myrtle influenced the content of essential elements in the raw materials: the growth of ferrum and zinc was facilitated by the overgrowth form of cultivation, and cuprum and manganese – by many years. The amount of ferrum in the phase of technological maturity of the leaf was 84.37 mg/kg: in leaf-growing leaves 2.67 times more than in raw materials with a long-term form of cultivation. For cuprum, the opposite tendency was obtained – during germination cultivation, its concentration decreased by 2 times. The culture and variety are characterized by a high amount of magnesium and calcium in the leaf mass, regardless of the method of cultivation, up to 3.3 times the daily requirement of a person. Scientific novelty. A distinctive feature of this culture and variety is its high manganese content (20.2 mg/kg) in comparison with other perennial crops growing in this area (Helichrysum, Lavandula, Elsholtzia).

Determination of the Component Composition of Essential Oil of Thyme Species by the Method of Gas Chromatography

2019 ◽  
Vol 74 (6) ◽  
pp. 310-314
Author(s):  
E. L. Malankina ◽  
L. N. Kozlovskaya ◽  
A. N. Kuzmenko ◽  
A. A. Evgrafov
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Component Composition

scholarly journals COMPONENT COMPOSITION OF ESSENTIAL OIL OF MONARDA FISTULOSA L. GRASS FROM THE COLLEC-TION OF NIKITSKY BOTANICAL GARDEN

2018 ◽  
pp. 55-62
Author(s):  
Ангелина (Angelina) Сергеевна (Sergeevna) Никитина (Nikitina) ◽  
Аслан (Aslan) Мурадалиевич (Muradalievich) Алиев (Aliyev) ◽  
Сергей (Sergej) Александрович (Aleksandrovich) Феськов (Feskov) ◽  
Наталья (Natal'ja) Владимировна (Vladimirovna) Никитина (Nikitina)
Keyword(s):  
Mass Spectrometry ◽  
Medicinal Plants ◽  
Essential Oil ◽  
Raw Materials ◽  
Botanical Garden ◽  
Component Composition ◽  
Raw Material ◽  
Monarda Fistulosa ◽  
Aboveground Part ◽  
Chromatography Mass Spectrometry

The creation of promising varieties of medicinal plants with useful properties is an actual direction. The acquisition of new highly productive varieties of medicinal plants and their introduction into the culture are successfully implemented in the Nikitsky Botanical Garden, R. Krym. The purpose of this work was to determine the specific features of the essential oil composition of the highly productive varieties Monarda fistulosa L. to determine the prospects of its use in pharmacy and further standardization of raw materials. Essential oil from the aboveground part of Monarda fistulosa L. was obtained by hydrodistillation using the Ginsberg instrument, its content in the raw material reached 2.38% in terms of absolutely dry raw materials. The component composition of essential oil from the aboveground part of the Monarda fistulosa L. was studied by chromatography-mass spectrometry. The presence of 41 components was revealed, 38 of them were identified, the main group being monoterpenes and their derivatives. The highest content of p-cymene, thymol, thymoquinone, thymohydroquinone, carvacrol, γ-terpinene was found in the sample of essential oil. Evaluation of the data of chromatography-mass spectrometry confirms the prospects of using essential oil and Monarda fistulosa L. grass of the assayed variety in pharmaceutical and medical practice as an antimicrobial, anti-inflammatory, antifungal and immunostimulating agent.

scholarly journals The component composition of the essential oil from Laurus nobilis L. unripe fruits harvested in Ukraine

2021 ◽  
Vol 19 (3(75)) ◽  
pp. 47-52
Author(s):  
Irina Yu. Posohova ◽  
Olha P. Khvorost ◽  
Kateryna S. Skrebtsova ◽  
Yuliia A. Fedchenkova
Keyword(s):  
Essential Oil ◽  
Component Composition ◽  
Biologically Active ◽  
Raw Material ◽  
Laurus Nobilis ◽  
Experimental Part ◽  
Quantitative Content ◽  
Skin Cleansing ◽  
The Village ◽  
Unripe Fruits

Aim. To study the qualitative composition and the quantitative content of the essential oil components from Laurus nobilis L. unripe fruits.Results and discussion. In the essential oil from Laurus nobilis L. unripe fruits 31 compounds were determined; among them 28 substances were identified. A high content was characteristic for spatulenol (1947.1 mg/kg) and betulenol (925.3 mg/kg).Experimental part. The raw material for obtaining the essential oil (unripe fruits) of Laurus nobilis L. was harvested in November 2017 in Alushta and the village of Rybalskoe, Crimea. The component composition of the essential oil of the unripe fruits from Laurus nobilis L. was studied and the constituents of the essential oil were identified by chromato-mass spectrometry using an Agilent Technology 6890N chromatograph. The component composition of the essential oil was revealed by comparing the results obtained with data from the NIST 02 mass spectrum library (more than 174.000 substances).Conclusions. The component composition of the essential oil from Laurus nobilis L. unripe fruits harvested in Ukraine has been studied. Taking into account the set of the biologically active compounds found in the essential oil of Laurus nobilis L. it can be argued that further pharmacognostic studies of fruits of this plant as the medicinal raw material with the predicted pharmacological activity (e.g., antimicrobial and skin cleansing) are promising.

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