scholarly journals COMPONENT COMPOSITION OF PHENOLIC COMPOUNDS AND TERPENOIDS OF THE ANGIOPROTECTIVE HERBAL COMPOSITION

2021 ◽  
pp. 145-155
Author(s):  
Vera Mikhaylovna Mirovich ◽  
Alina Alekseyevna Posokhina ◽  
Svetlana Andreyevna Petukhova ◽  
Daniil Nikolayevich Olennikov ◽  
Lyubov' Vissarionovna Dudareva
Keyword(s):  
Phenolic Compounds ◽  
Essential Oil ◽  
Total Content ◽  
Component Composition ◽  
Retention Indices ◽  
Gas Chromatography Mass Spectrometry ◽  
Triterpene Saponins ◽  
Phenolcarboxylic Acid ◽  
Linear Retention Indices ◽  
Beijing China

The study of the component composition of phenolic and terpenoid compounds of the angioprotective herbal composition was carried out. Alcohol extraction of the angioprotective herbal composition was studied by MC-HPLC-UV method. The analysis used solutions of commercial samples of reference substances manufactured by Sigma-Aldrich (USA), Chem-Fages, Extrasynthese, Lione (France), Beijing (China). Seven phenolic compounds have been identified: quercetin, isoramnetin, rutin, isoquercitrin, narcissin, isoramnetin-3-O-glucoside, phenolcarboxylic acid 5-O-caffeylquinic. The total content of flavonoids is 11.64 mg/g, phenolcarboxylic acid – 2.30 mg/g. Among the isolated flavonoids rutin (3.35±0.06 mg/g), isoquercitrin (3.14±0.06 mg/g), narcissin (4.15±0.09 mg/g), phenolcarboxylic acid – 5-O-caffeylquinic (2.30±0.05 mg/g) prevail. The essential oil was obtained by hydrodistillation; analysis was performed by gas chromatography-mass spectrometry on an Agilent Technologies (6890N) instrument with a quadrupole mass spectrometer. The identified components were performed by comparing the linear retention indices and total mass spectra of the compounds with the data from the Nist 11 library and commercial samples. In the herbal composition of the essential oil, 21 components have been identified, the main of which are salicylic aldehyde (in the total amount is 58.30%), methyl salicylate (16.17%). The triterpene saponins of the angioprotective herbal composition are represented by calendulosides A and B, escin. The amount of triterpene saponins is 1.08±0.05%. The results of the quantitative analysis were processed statistically, the data are presented as the mean and ± standard deviation, SD.

Comparative analysis of essential oil obtained from fresh and dried leaves of medicinal sage

2020 ◽  
pp. 39-53
Author(s):  
Tatyana Shcheglova
Keyword(s):  
Comparative Analysis ◽  
Essential Oil ◽  
Raw Materials ◽  
Quantitative Difference ◽  
Botanical Garden ◽  
Component Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
Qualitative And Quantitative ◽  
Salvia Officinalis L ◽  
Medical University

The article presents results of the study of a comparative analysis of essential oil obtained from fresh and dried leaves of medicinal sage (Salvia officinalis L.), growing in the Botanical Garden of the First Moscow State Medical University named after Sechenov. The studies were carried out using gas chromatography– mass spectrometry. Qualitative and quantitative difference in the component composition of the essential oil obtained from fresh and dried raw materials was determined as a result of the study.

scholarly journals STUDYING THE CHEMICAL COMPOSITION OF ESSENTIAL OIL RECEIVED FROM FRUITS OF PRANGOS ODON-TALGICA WILD-GROWING IN ASTRAKHAN REGION

2019 ◽  
pp. 95-101
Author(s):  
Anatoliy Valeriyevich Velikorodov ◽  
Vladimir Nikolayevich Pilipenko ◽  
Tat'yana Alekseyevna Pilipenko ◽  
Sergey Viktorovich Malyi
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Solid Phase ◽  
Raw Materials ◽  
Retention Indices ◽  
Gas Liquid Chromatography ◽  
Linear Retention Indices ◽  
Chromatographic Peaks ◽  
Main Components ◽  
Methods Of Isolation

The chemical composition of Prangos odontalgica (Pall.) Herrnst. et. Heyn essential oil, which grows wild in the Astrakhan region, has not been practically studied, unlike other species of the genus Prangos, growing in Iran, Turkey and Uzbekistan. To obtain the essential oil, all parts of the plant (roots, stems, flowers, leaves, fruits) are used, as well as various methods of isolation (hydrodistillation, supercritical fluid extraction, solid-phase microextraction, and others). Essential oil from the fruit of Prangos odontalgica we obtained by the method of hydrodistillation. The duration of the hydrodistillation process, established experimentally on the basis of a study of the dynamics of change in the yield of essential oil over time, was 3 hours. The yield of essential oil in mass% by volume in terms of absolutely dry raw materials according to the results of a threefold determination is 0.07 ± 0.01%. The method of gas-liquid chromatography carried out a quantitative analysis of the main components of the essential oil Prangos odontalgica. The quantitative content of the components of the essential oil was calculated by the areas of gas chromatographic peaks without the use of correction factors. Qualitative analysis was performed by comparing linear retention indices. In the Prangos odontalgica essential oil, 38 substances were identified (88.4%). It contains sesquiterpenes (31.48%), terpenoids (19.2%), hydrocarbons (15.39%), carboxylic acids and their esters (10.86%), alcohols, phenols (8.84%). Among sesquiterpenes, the predominant components are γ-elemen (9.84%), bisabolol (9.41%), and among terpenoids – trans-nerolidol (3.90%) and linalyl isobutyrate (3.41%).

scholarly journals Essential Oil of Flowers of Anaphalis contorta, an Aromatic and Medicinal Plant from India

2013 ◽  
Vol 8 (2) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Rajesh K. Joshi
Keyword(s):  
Mass Spectrometry ◽  
Essential Oil ◽  
Mass Spectra ◽  
Capillary Column ◽  
Retention Indices ◽  
Gas Chromatography Mass Spectrometry ◽  
Relative Retention ◽  
Sesquiterpene Hydrocarbons ◽  
Total Oil ◽  
Polar Stationary Phase

The hydro-distilled essential oil of dried flowers of Anaphalis contorta Hook f. has been examined by means of gas chromatography-mass spectrometry (GC- MS). The oil constituents were identified according to their mass spectra and their relative retention indices determined on a non-polar stationary phase capillary column. Fifty-one constituents have been identified representing 97.0% of the total oil. The major constituents were β-caryophyllene (19.2%), γ- curcumene (17.5%), δ-cadinene (10.2%), labda-7,14-dien-13-ol (4.8%), epi-α-cadinol (4.3%), bulnesol (4.3%), α-cadinol (3.8%), β-bisabolol (3.7%) and labda-8,14-dien-13-ol (3.3%). The oil was rich in sesquiterpene hydrocarbons (60.5%), followed by oxygenated sesquiterpenes (26.6%), oxygenated diterpenes (8.1%) and diterpene hydrocarbons (1.8%).

scholarly journals COMPARATIVE ANALYSIS OF ESSENTIAL OIL THE VEGETABLE RAW MATERIAL SCHISANDRA CHINENSIS: LEAVES, WOODY STEMS, RHIZOMES WITH ROOTS

2019 ◽  
pp. 173-180
Author(s):  
Юрий (Yuriy) Алексеевич (Alekseevich) Морозов (Morozov) ◽  
Ифрат (Ifrat) Назимович (Nazimovich) Зилфикаров (Zilfikarov) ◽  
Елизавета (Elizaveta) Владимировна (Vladimirovna) Морозова (Morozova) ◽  
Аслан (Aslan) Мурадалиевич (Muradalievich) Алиев (Aliev) ◽  
Тимур (Timur) Алгасанович (Algasanovich) Ибрагимов (Ibragimov)
Keyword(s):  
Comparative Analysis ◽  
Essential Oil ◽  
Essential Oils ◽  
Raw Materials ◽  
Component Composition ◽  
Raw Material ◽  
Gas Chromatography Mass Spectrometry ◽  
Schisandra Chinensis ◽  
Marker Substances ◽  
Vegetable Raw Materials

This paper presents the results of an experimental study of the component composition of essential oil samples obtained from various vegetable raw materials of Schisandra chinensis (Schisandra chinensis (Turcz.) Baill.): branches (woody stems), rhizomes with roots and leaves. The extraction of essential oils was carried out by distillation with water vapor followed by extraction from the distillate with ethoxyethane. The largest yield of essential oil in terms of completely dry raw material is registered from rhizomes with roots – 0.99%; the yield from leaves and branches was approximately the same: 0.51% and 0.52%, respectively. A comparative analysis of the component composition of the obtained essential oils was carried out using the gas chromatography / mass spectrometry method. In the essential oil of the leaves 29 detected compounds (identified by 21 connection; dominant components: Cyclohexylmethyl ether sulphurous acid, (+)-trans-nerolidol, δ-cadinene, Tridec-(2E)-EN-1-ol), woody stems 80 connections (identified 65 compounds; dominant components: ß-pinene, Camphene, Bornylacetate, (+)-trans-nerolidol, p-tsimen, Tridecan-2-it, δ-cadinene), rhizomes with roots 78 of the compounds (60 compounds identified; dominant components: δ-cadinene, Bornylacetate, β-pinene, Camphene, Nerolidol-(E), Tridecan-2-it, γ-cadinene, Borneol). For each sample of the studied essential oils selectivity and marker substances were established.

scholarly journals STUDY OF CHEMICAL COMPOSITION OF TAMARIX RAMOSISSIMA ESSENTIAL OIL

2017 ◽  
pp. 117-121 ◽  
Author(s):  
Анатолий (Anatolij) Валериевич (Valerievich) Великородов (Velikorodov) ◽  
Владимир (Vladimir) Николаевич (Nikolaevich) Пилипенко (Pilipenko) ◽  
Татьяна (Tat'jana) Алексеевна (Alekseevna) Пилипенко (Pilipenko) ◽  
Алексей (Aleksej) Георгиевич (Georgievich) Тырков (Tyrkov)
Keyword(s):  
Essential Oil ◽  
Steam Distillation ◽  
Retention Indices ◽  
Raw Material ◽  
Tamarix Ramosissima ◽  
Gas Liquid Chromatography ◽  
Vegetative Period ◽  
Sesquiterpene Hydrocarbons ◽  
Linear Retention Indices ◽  
Main Components

Samples of essential oil from Tamarix ramosissima plant growing in the Astrakhan region were obtained by steam distillation and the dependence of its yield on the vegetative period of the plant was studied. The duration of the process of steam distillation was established experimentally on the basis of a study of the dynamics of the change in the yield of essential oil in time. The yield of essential oil was determined in % by weight of absolutely dry raw material. The largest yield of essential oil was obtained from plants in the flowering phase (0,23–0,3%). By method of gas-liquid chromatography carried out a quantitative analysis of the main components of essential oil Tamarix ramosissima. The quantitative content of essential oil components was calculated from the areas of gas chromatography peaks without the use of correction factors. Qualitative analysis was carried out by comparing the linear retention indices. It was found that the composition of the essential oil of Tamarix is very specific. It includes hydrocarbons (9,51%), alcohols (3,46%), aldehydes (2,55%), monoterpene hydrocarbons (5,4%), oxygenated terpenes (23,06%), sesquiterpene hydrocarbons (1,48%), oxygenated diterpenoids (3,37%), and a significant amount of fatty acids and their esters (45,43%). The main components of the essential oil are tetrahydrogeranilacetone (21,35%), nonane (12,63%), decanoic (9,82%) acids and dihydroactin diolide (5,40%).

scholarly journals STUDY OF COMPONENT COMPOSITION AND ANTIMICROBIAL ACTIVITY OF ACHILLEA NOBILIS L. ESSENTIAL OIL

2021 ◽  
Vol 91 (1) ◽  
pp. 36-41
Author(s):  
E. G. Kerimli ◽  
S. V. Serkerov
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Component Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
High Antimicrobial Activity ◽  
Spectrometry Method ◽  
Mass Spectrometry Method ◽  
First Time

The component composition of nobleyarrow L. essential oil growing in Azerbaijan was studied for the first time by gas chromatography-mass spectrometry method. 35 components, mainly terpenoid compounds, are identified in the essential oil of which the main ones are 15 components: artemisia ketone (23,7%), α-thujone (22,4%), 2-bornanone (6,4%), eudesma-7(11)-en-4-ol (6,3%), eucalyptol (4,5%), cubenol (3,3%), lavandulol (3,0%), β-thujone (2,9%), β-eudesmol (2,7%), methyl hinokiate (2,1%), terpinene-4-ol (1,7%), 1,2-longidione (1,3%), limonen-6-ol, pivalate (1,2%), neryl (S)-2-methylbutanoate (1,1%), сaryophyllene oxide (1,0%). As a result of noble yarrow essential oil research it was determined that, essential oil has high antimicrobial activity relative to Staphylacoccus aureus.

scholarly journals Features of the Bacillus thuringiensis 888 strain’s influence on the content of essential oil, antioxidants and flavonoids in Origanum vulgare L.

2021 ◽  
Vol 11 (3) ◽  
pp. 430-440
Author(s):  
A. V. Kryzhko ◽  
U. M. Budzhurova ◽  
E. D. Ametova ◽  
I. A. Novikov ◽  
E. E. Soboleva ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Essential Oil ◽  
Oil Content ◽  
Total Content ◽  
Component Composition ◽  
Water Soluble ◽  
Soluble Carbohydrates ◽  
Origanum Vulgare ◽  
Reduction Of Iron ◽  
Spore Culture

Abstract: This article aims to study the influence of the culture of the Bacillus thuringiensis var. thuringiensis 888 on the quality of the vegetative mass of Origanum vulgare in terms of the number of antioxidants and flavonoids, yield and composition of the essential oil. The research material included a liquid spore culture of the B. thuringiensis 888 strain, samples of oregano: sample no. g-4, containing 52.0% carvacrol in essential oil; No. 2 containing 59.85% α-terpineol; No. 1 with a predominant content of germacrene D (21.5%) and β- caryophyllene (19.4%). Soluble carbohydrates in the plants were determined using M.S. Dubois’s method, flavonoids – spectrophotometrically at 420 nm following R.A. Bubenchikov’s method. The total content of antioxidants was determined using the reduction of iron(III) chloride to iron(II) chloride. The essential oil content of Origanum vulgare was determined by using hydrodistillation following A.S. Ginsberg. Gas chromatography was used to determine component composition of the essential oil. The results show that treating the O. vulgare samples with the spore culture of B. thuringiensis 888 strain culture does not significantly affect the accumulation of terpenoid quinones, tochromanols and water-soluble antioxidants in leaves. It has been shown that treating oregano with an ordinary liquid spore culture of the B. thuringiensis 888 strain promotes the formation of a persistent tendency towards the accumulation of reducing sugars in the vegetative mass of plants — up to 30.8% compared to the control. Treating plants with a culture of B. Thuringiensis 888 strain promoted an increase in the essential oil content in O. vulgare plants of the sample no. 1 by 2.4 times, as compared to the control and did not significantly affect the essential oil content of the samples no. 2 and no. g-4. The sample no. g-4 was the most resistant to treatment with entomopathogenic bacteria, and the content of linalool and caryophyllene oxide in essential oils decreased by 44.6 and 37.1%, respectively, and linalyl acetate by 4.3 times compared with the control, as well as the accumulation of α-terpineol by 86.1%.

scholarly journals THE EFFECT OF THE USE OF GROWTH REGULATORS ON THE COMPONENT COMPOSITION OF ESSENTIAL OIL OF THE TOPS AND SEEDS OF ANETHUM GRAVEOLENS L.

2017 ◽  
pp. 217-221
Author(s):  
Ольга (Ol'ga) Владимировна (Vladimirovna) Шелепова (Shelepova) ◽  
Тамара (Tamara) Ивановна (Ivanovna) Хуснетдинова (Husnetdinova)
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Essential Oil ◽  
Growth Regulators ◽  
Component Composition ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Anethum Graveolens ◽  
Spectrometry Analysis ◽  
Main Components

The content and component composition of essential oil of air-dried phytomass and seeds of Anethum graveolens L. were determined. Dill was grown using the organo-mineral preparation Ideal and steroid phytohormone (24-epibrassinolide) Epin-extra. The use of growth regulators increased the yield of green dill (Ideal by 16%, Epin-extra by 6% compared to the control); did not affect the content of essential oil in the herbs of dill and increased the content of essential oil of dill seeds. GC/MS (Gas Chromatography / Mass Spectrometry) analysis revealed differences the ratio of the main components of the essential oil of dill when the use of growth regulators. In the essential oil of air-dried phytomass of plants, compared with the control, the content of alpha-fellandrene (1,3–1,7 times) and limonene (1,1–1,2 times) increased, anethofuran (1,4–1,8 times) and carvone (in 1,4–1,5 times). In the essential oil of dill seeds - increased content of limonene (1,7–1,9 times) and decreased carvone (1,1 times). When using growth regulators, a change in the aroma of plants was observed. The use of the Epin-extra drug caused more significant changes in the content of the major components of the essential oil of dill.

scholarly journals Phenolic compounds of Bistorta alopecuroides (Turcz. ex Meissn.) Kom. (Polygonaceae) from different populations of Siberia and the Far East

2018 ◽  
Vol 11 ◽  
pp. 00047
Author(s):  
Maria Voronkova ◽  
Galina Vysochina
Keyword(s):  
Phenolic Compounds ◽  
Far East ◽  
Total Content ◽  
Component Composition ◽  
Plant Metabolism ◽  
Technogenic Pollution ◽  
High Mountains ◽  
The Far East ◽  
Different Populations ◽  
Adaptive Role

The article considers research of the phenolic compounds composition and content in leaves of Bistorta alopecuroides (Turcz. Ex Meissn.) Kom. from different populations using HPLC methods. It shows that B. alopecuroides component composition is quite variable and depends on many environmental factors, such as microclimate, altitude, technogenic pollution. Isovitixin, rutin and flavonolglycoside (tR = 9.2 min; UV 273, 355 nm) compounds are found in B. alopecuroides leaves of all studied populations. B. alopecuroides growing in high mountains and under technogenic pollution conditions have the highest content of phenolic compounds, which confirms their adaptive role in plant metabolism. The total content of phenolic compounds in leaves of B. alopecuroides plants reaches 20.82mg/g.

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