scholarly journals Spectroscopic and elementary characterization of humic substances in organic substrates

2018 ◽  
Vol 9 (2) ◽  
pp. 264-274 ◽  
Author(s):  
Rubens Ribeiro Da Silva ◽  
Guilherme Nunes Lucena ◽  
Ângela Franciely Machado ◽  
Gilson Araújo De Freitas ◽  
Antônio Teixeira Matos ◽  
...  
Keyword(s):  
Humic Acid ◽  
Humic Substances ◽  
Visible Region ◽  
Exchange Capacity ◽  
Organic Substrate ◽  
Organic Substrates ◽  
Molecular Absorption ◽  
Charge Generation ◽  
Elementary Analysis ◽  
Carbon Acid

The humic substances, major constituents of the organic matter of the substrates, play an important role in plant development, including: the availability of nutrients, the generation of negative charges (CEC) and the buffering effect. Based on this, this study aimed to use the molecular absorption spectroscopy in the ultraviolet-visible region (UV-VIS), the vibrational spectroscopy Fourier transform infrared (FTIR) and the elementary analysis CHN/O techniques to characterize the humic acid (HA) and fulvic acid (FA) fractions of the humic substances of five organic substrates, named as T1, T2, T3, T4, and T5, which were produced at the Federal University of Tocantins – Campus Gurupi. The molecular absorption spectra in UV-VIS and FTIR on the T1, T2, T3, T4 and T5 substrates showed humification, the formation of negative charges, which is related to the cation exchange capacity (CEC) and the complexation of metal ions that are considered nutrients. The biggest humification was shown for the T4 substrate, in function of its higher cabon-acid humic/carbon-acid fulvic ratio and the lower C/N ratio, allied to a negative charge generation, the low E4/E6 ratio found in the humic acid fraction. The complexation of metal nutrient ions indicates the greatest potencial of its use for seedlings production and the conservation of the chemical and physical properties of the organic substrate. 

Humic substances affecting the limitation of the activated sludge process for removal of micropollutants

1998 ◽  
Vol 38 (7) ◽  
pp. 217-225 ◽  
Author(s):  
Saburo Matsui ◽  
Hiroshi Yamamoto ◽  
Yoshihisa Shimizu ◽  
Jun Harada ◽  
Daisuke Einaga
Keyword(s):  
Humic Acid ◽  
Activated Sludge ◽  
Humic Substances ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Removal Rate ◽  
Natural Environments ◽  
Organic Substrates ◽  
Activated Sludge Process ◽  
Municipal Wastewater Treatment

In our previous investigation with the Bacillus subtilis rec-assay, a strong DNA-damaging potential was found in effluents of municipal wastewater treatment plants and nightsoil treatment plants using the activated sludge process. We hypothesized that the presence of non-biodegradable humic substances in the influent and the association of micropollutants inducing DNA-damaging chemicals to them may cause the comparatively lower removal rate of micropollutants than that of primary organic substrates. In this research, a batch type activated sludge process was set up in the laboratory to examine the fate of these toxic micropollutants and to evaluate the effect of humic substances. As neutral and polar micropollutants, pyrene and 1-aminopyrene was respectively selected, and their speciation (i.e., free, sorbed onto dissolved organic matters including humic acid, and sorbed onto activated sludge) was investigated. Almost all pyrene was sorbed onto activated sludge without any biodegradation. It was observed that the effect of humic substances made both pyrene and 1-aminopyrene leave the process in forms sorbed onto humic acid and free in the effluent. This complexity of the fate of micropollutants and the significant effect of humic acid leads to the necessity of further investigation not only in the activated sludge process but also into the other various natural environments.

Dynamics of phosphorus and organic substrates in anaerobic and aerobic phases of a sequencing batch reactor

1994 ◽  
Vol 30 (6) ◽  
pp. 237-246 ◽  
Author(s):  
A. Carucci ◽  
M. Majone ◽  
R. Ramadori ◽  
S. Rossetti
Keyword(s):  
Sequencing Batch Reactor ◽  
Municipal Wastewater ◽  
Batch Reactor ◽  
General Assumption ◽  
Organic Substrate ◽  
Operating Conditions ◽  
Phosphate Removal ◽  
Substrate Uptake ◽  
Organic Substrates ◽  
Polyphosphate Metabolism

This paper describes a lab-scale experimentation carried out to study enhanced biological phosphate removal (EBPR) in a sequencing batch reactor (SBR). The synthetic feed used was based on peptone and glucose as organic substrate to simulate the readily biodegradable fraction of a municipal wastewater (Wentzel et al., 1991). The experimental work was divided into two runs, each characterized by different operating conditions. The phosphorus removal efficiency was considerably higher in the absence of competition for organic substrate between P-accumulating and denitrifying bacteria. The activated sludge consisted mainly of peculiar microorganisms recently described by Cech and Hartman (1990) and called “G bacteria”. The results obtained seem to be inconsistent with the general assumption that the G bacteria are characterized by anaerobic substrate uptake not connected with any polyphosphate metabolism. Supplementary anaerobic batch tests utilizing glucose, peptone and acetate as organic substrates show that the role of acetate in the biochemical mechanisms promoting EBPR may not be so essential as it has been assumed till now.

scholarly journals Determination of Organic Compounds, Fulvic Acid, Humic Acid, and Humin in Peat and Sapropel Alkaline Extracts

Molecules ◽  
2021 ◽  
Vol 26 (10) ◽  
pp. 2995
Author(s):  
Laurynas Jarukas ◽  
Liudas Ivanauskas ◽  
Giedre Kasparaviciene ◽  
Juste Baranauskaite ◽  
Mindaugas Marksa ◽  
...  
Keyword(s):  
Humic Acid ◽  
Fulvic Acid ◽  
Humic Substances ◽  
Organic Compounds ◽  
Chemical Constituents ◽  
Acid Amide ◽  
Extraction Methods ◽  
Ultrasound Extraction ◽  
Different Origins

Black, brown, and light peat and sapropel were analyzed as natural sources of organic and humic substances. These specific substances are applicable in industry, agriculture, the environment, and biomedicine with well-known and novel approaches. Analysis of the organic compounds fulvic acid, humic acid, and humin in different peat and sapropel extracts from Lithuania was performed in this study. The dominant organic compound was bis(tert-butyldimethylsilyl) carbonate, which varied from 6.90% to 25.68% in peat extracts. The highest mass fraction of malonic acid amide was in the sapropel extract; it varied from 12.44% to 26.84%. Significant amounts of acetohydroxamic, lactic, and glycolic acid derivatives were identified in peat and sapropel extracts. Comparing the two extraction methods, it was concluded that active maceration was more efficient than ultrasound extraction in yielding higher amounts of organic compounds. The highest amounts of fulvic acid (1%) and humic acid and humin (15.3%) were determined in pure brown peat samples. This research on humic substances is useful to characterize the peat of different origins, to develop possible aspects of standardization, and to describe potential of the chemical constituents.

Soil Augmentation by Humus: Replenishing lost soils and supplementing new ones

2021 ◽  
Author(s):  
Hemlata Bagla ◽  
Asma Khan
Keyword(s):  
Organic Matter ◽  
Humic Acid ◽  
Plant Growth ◽  
Humic Substances ◽  
Soil Degradation ◽  
Lunar Regolith ◽  
Radioactive Waste Disposal ◽  
Saline Stress ◽  
Plant Metabolites ◽  
Growth Phenomenon

<p>Earth’s regolith consists of a vital component that is lacking on other planets ­­– the pedosphere or soil body – that is rich in organic matter, soil fauna, minerals, water, gases, that together support life and is thus essential for plant growth. In stark contrast to our blue planet, Martian regolith is devoid of organic matter and contains crushed volcanic rocks, with high mineral content and toxic chemicals like perchlorates. Nevertheless, Martian and Lunar regolith simulants formulated by NASA, have been experimented for crop growth by addition of organic matter suitable to bind xenobiotics and provide ample nutrients, as an essential step towards expanding our horizon in the extensive field of soil sciences.</p><p>Soil is an ecosystem as a whole and acts as a modifier of planet Earth’s atmosphere. The organic matter present in it originates mainly from plant metabolites with the onset of senescence and humification. Humic substances thus formed in the pedosphere exhibit exceptional characteristics for soil conditioning. Besides providing nutrients and aeration to the soil, they interact and bind with toxic heavy metals, radionuclides, pesticides, industrial dyes, and other xenobiotics that may be present as pollutants in the ecosystem, thus acting as natural sieves. As top soils have maximum organic matter, essential for plant growth, phenomenon like soil erosion leave the soils devoid of humic substances. Another major reason for soil degradation is excessive salinity, leading to osmotic and ionic stress in plants, eventually reducing their growth. Addition of humic acid in soils provides protection against high saline stress and minimizes yield losses. In India, one of the leading agrarian countries, it is a common practice to enrich soils with manure, which is an inexpensive form of humus-boost for the crops. Such practices aid the cyclic flow of organic matter in the environment, against the background of widespread soil degradation.</p><p>Another global form of soil degradation is radioactive contamination of soils which occurs mainly due to nuclear accidents and improper practices of radioactive waste disposal. In order to explore such interactions with humic acid following Green technique, batch biosorption studies were performed over a range of parameters, with radionuclides Cs and Sr that are found in low level radioactive wastes. Biosorption percentages of 91±2% and 84±1% were obtained for Cs and Sr respectively. The technique is chemical-free and emphasizes the ‘nature for nature’ outlook of solving environmental problems. Humic acid and its various forms thus act as traps for radionuclides and work as excellent restorative soil stimulants that supplement depleted soils, boost plant growth, and play a vital role in sustaining life on Earth.</p>

Bioavailability of a nonylphenol isomer in dependence on the association to dissolved humic substances

2004 ◽  
Vol 50 (5) ◽  
pp. 277-283 ◽  
Author(s):  
R. Vinken ◽  
A. Höllrigl-Rosta ◽  
B. Schmidt ◽  
A. Schäffer ◽  
P.F.-X. Corvini
Keyword(s):  
Humic Acid ◽  
Carbon Source ◽  
Humic Substances ◽  
Organic Compounds ◽  
Sole Carbon Source ◽  
Equilibrium Dialysis ◽  
Fulvic Acids ◽  
Degrading Bacterium ◽  
Bacterium Strain ◽  
Dissolved Humic Substances

Humic substances are important environmental components since they represent a very large part of organic compounds on earth. According to many reports, dissolved humic substances are a determinant parameter for the bioavailability of xenobiotic compounds. For the present bioavailability studies, two kinds of dissolved humic substances, a commercially available humic acid and fulvic acids isolated from peat were used. As the relevant xenobiotic, a defined branched nonylphenol isomer, 4(3′,5′-dimethyl-3′-heptyl)-phenol (p353NP) was synthesised according to Friedel-Crafts alkylation. Equilibrium dialysis studies were implemented in order to investigate the association between 14C-labelled p353NP and dissolved humic substances. The biodegradability in the presence of dissolved humic substances was examined in experiments with the nonylphenol degrading bacterium strain Sphingomonas TTNP3 and with p353NP as sole carbon source. The results showed that p353NP-humic acid associates were formed in high amounts, whereas no adducts with fulvic acids occurred. In the degradation studies with Sphingomonas TTNP3, no effects of dissolved humic substances on the bioavailability of p353NP could be observed. It was assumed that the association between nonylphenol and humic acids occurs rapidly and is reversible. Thus, the formation of "labile" complexes did not influence biodegradation rates, which were quite low.

scholarly journals Study on evaluating the effectiveness of compost fertilizer from jackfruit peel and fiber with various local agricultural materials on Green Mustard (Brassica juncea)

2021 ◽  
Vol 332 ◽  
pp. 07001
Author(s):  
Thanh Tran ◽  
Van Thi ◽  
Tran Thi Bich Phuong ◽  
Loc Huu Ho ◽  
Le Thi Anh Hong
Keyword(s):  
Germination Rate ◽  
Organic Fertilizer ◽  
Control Sample ◽  
Organic Substrate ◽  
National Standards ◽  
Organic Carbon Content ◽  
Organic Substrates ◽  
Coconut Fiber ◽  
Three Samples ◽  
Agricultural Materials

In recent years, jackfruit production in Vietnam has been growing very strongly in both quantity and quality. However, most of the jackfruit is harvested and processed for meat, the rest is the jackfruit peels, and fibers are discarded, which will affect environmental sanitation. The study aims to enhance the value of jackfruit by taking advantage of nutrient ingredients in the peel and fiber of jackfruit to blend into organic fertilizer. Initial results show that the compost substrate samples mixed with rice husk ash and coir and the organic substrate sample mixed with coconut fiber had the best quality. Tested nutritional ingredients include including humidity is 76.1%, total organic carbon content is 27.3%, fulvic acid is 0.9%, humid acid is 0.9%, total nitrogen is 0.85%, total phosphorus is 0.57% after 35 days of incubation. Their quality meets the national standards of QCVN 01-189: 2019/BNNPTNT on fertilizer quality. After testing all three samples of organic substrates on the green mustard with germination rate (%), a number of branches, and total fresh weight (g), the results showed that test plants are grown with the compost from the shell - jackfruit fiber mixed with coconut fiber developed better than the control sample.

Low Stress Under Bump Metallizations for Direct Chip Attach

1998 ◽  
Vol 555 ◽  
Author(s):  
P. Su ◽  
T. M. Korhonen ◽  
S. J. Hong ◽  
M. A. Korhonen ◽  
C. Y. Li
Keyword(s):  
Flip Chip ◽  
Semiconductor Industry ◽  
Organic Substrate ◽  
Organic Substrates ◽  
Under Bump Metallization ◽  
Interfacial Failure ◽  
Stress Measurements ◽  
Rapid Reaction

AbstractIn order to use a flip chip method for bonding the Si chip directly to an organic substrate, compatible under bump metallization (UBM) must be available. Conventional schemes with a copper-based solderable layer are not well compatible with the high-tin solders (such as eutectic Pb-Sn) used with organic substrates. This is due to the rapid reaction between Sn and Cu which depletes the UBM of copper. Ni-based schemes exhibit slower reaction with the solder and have been identified by the semiconductor industry as preferable replacements to Cu-based UBM's. However, Ni-containing metallurgies are often associated with high stresses, which results in poor practical adhesion between the silicon chip and the metallization, leading to interfacial failure during fabrication or service. In this research, several nickel-containing UBM schemes are studied experimentally. Stress measurements are made for each metallization before patterning of UBM pads. An optimal Ni concentration for the UBM is suggested based on the results from this study.

EFFECT OF HUMIC AND FULVIC ACIDS ON MICROBIAL GROWTH

2021 ◽  
Author(s):  
Milanka Radulovic ◽  
◽  
Svetlana Mitrovski
Keyword(s):  
Humic Acid ◽  
Humic Substances ◽  
Minimal Medium ◽  
Microbial Growth ◽  
Growth Curves ◽  
Fulvic Acids ◽  
Humic And Fulvic Acids ◽  
Staphyloccocus Aureus ◽  
Low Ionic Strength ◽  
Strength Medium

Peat is a natural substrate for growth of microorganisms because it is rich in compounds that microorganisms can use as sources of carbon, nitrogen and growth factors. Peat originating from Vlasina lake in Eastern Serbia is especially rich in organic matter. The content of humic substances (humic acid, fulvic acid and humine) is almost twice that found in other peat-rich regions of similar origin and geochemical age. Humic and fluvic acids are known to promote microbial growth. In this work, humic and fulvic acids were first extracted from Vlasina lake peat and then added to minimal medium (synthetic, low ionic strength medium). The humic substances were added separately and combined in a 1:1 ratio by mass to study their individual and combined effect on microbial growth of Escherichia coli ATCC 25922 (Gr–), Staphyloccocus aureus (Gr+) i Aureobasidium pullulans, strain CH-1. The microbial growth was measured microspectrophotometrically over a 24-hour period and growth curves were obtained for a range of acid concentrations between 25 µg cm-3 and 100 µg cm-3. It was found that both humic and fulvic acids promote the growth of all three microorganisms by up to a maximum of 40%-80% the extent of which varied with the concentration of the acid and the identity of the microorganism. In general, humic acid was found to result in higher microbial growth (at highest concentrations, up to ~80% for all three microbial species).

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