Cytogenetic effects of tick-borne encephalitis in residents of the north of Western Siberia with alimentary-constitutional obesity depending on the polymorphism of the genes of glutathione-S-transferase

Backgraund. Tick-borne encephalitis (TBE), one of the large-scale public health problems of the Siberian region, can cause significant cytogenetic damage in humans by stimulating oxidative stress. Active functioning of the detoxification system including enzymes of glutathione-S-transferase is intended to protect the genetic structure of cells in the body, while at the same time, there are studies showing the defective functioning of this system in individuals with obesity. Aims. Aim of this work is to study the longevity of term of TBE-induced cytogenetic damage in patients with different alleles of genes of glutathione-S-transferase, suffering from alimentary-constitutional obesity compared with the control. Materials and methods. We were examined 133 female residents of the northern areas of Tomsk region from 35 to 44 years old who were hospitalized for infection TBE at local medical clinics. All individials were divided into two subgroups. Patients of the 1st subgroup (control) had a body mass index (BMI) within 2126, the 2nd subgroup had BMI showing severe obesity (BMI 3545). Buccal cells for cytogenetic and molecular analysis were taken several times: 12 days after hospitalization, 1 week, 1 month, 3 months, and 6 months after beginning of the disease. Methods of immunoassay and polymerase chain reaction (PCR) was used to confirm the diagnosis of TBE. As an untreated control were examined 126 healthy female residents of the same age groups. All individuals signed informed consent. In the analysis of deletions in the genes GSTM1 and GSTT1 was used multiplex PCR. We studied at least 1000 epithelial buccal cells from each individual. Results. In patients with high BMI rates revealed a significant increase in the frequency of the buccal cells with cytogenetic damage, compared to the control group. TBE disease was significantly increased the frequency of the cytogenetically damaged cells in this group of patients. The cytogenetic aberrations persisted in their body for six months after hospitalization. In patients with normal BMI rates TBE induced significantly less changes. The recovery of normal cytogenetic status was observed in this group 3 months after hospitalization. Analysis of the glutathione-S-transferase gene variants showed that in TBE patients with normal BMI and non-active alleles of these genesthe frequency of micronucleated epithelial cellswere significantly increased compared to the patients with the activeGSTM1 (+) / GSTT1 (+)genes. In the patients with alimentary obesity such patterns were not observed. Conclusions. Thus,infection with TBE in patients with obesity induced significant increase in both the frequency of cytogenetically damaged cells and the longevity of their persistency in the body compared to these in control group. Polymorphisms in genes of glutathione-S-transferase had no significant effect on the indices.

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Monitoring of cytogenetic instability by micronuclei assay of both immunocompetent and non-immunocompetent cells in tick-borne encephalitis patients depending on variants of glutathione-S-transferase genes in the genotype

Russian Journal of Infection and Immunity ◽

10.15789/2220-7619-2019-3-4- ◽

2019 ◽

Vol 9 (3-4) ◽

pp. 600-606

Author(s):

Nikolay Nikolaevich Ilyinskikh ◽

Ekaterina Nikolaevna Ilyinskikh ◽

Evgenia Vladimirovna Zamyatina ◽

Svetoslava Vyacheslavovna Lee

Keyword(s):

T Lymphocytes ◽

Peripheral Blood ◽

Micronucleus Assay ◽

Immunocompetent Cells ◽

Control Group ◽

Buccal Cells ◽

Glutathione S Transferase ◽

Tick Borne Encephalitis ◽

Buccal Mucous Membrane ◽

The One

Aim of this study was to study the dynamics of the frequency of cytokinesis-blocked T-lymphocytes with micronuclei in peripheral blood and the frequency of buccal micronucleated epithelium cells for a period of half a year in patients with acute tick-borne encephalitis, depending on burden of active and inactive variants of glutathione-S-transferase genes (GSTM1 and GSTT1) in the patient's genotype. We carried out micronucleus assay in immunocompetent and non-immunocompetent cells in 54 patients with acute tick-borne encephalitis and 35 healthy persons (control) residing in the Tomsk and Tyumen regions. To analyze the frequency of cytokinesis-blocked micronucleated T-lymphocytes was used venous peripheral blood as material for phytohemagglutinin-stimulated cultures, and to study the frequency of buccal micronucleated cells, samples of the buccal mucous membrane epithelial cells were obtained. To carried out both techniques of micronucleus assay, cytological preparations were prepared, which were stained using the Giemsa or Felgen methods. The material for the study was obtained repeatedly during admission of patients to treatment, and also after 1 week, 1, 3 and 6 months. Polymerase chain reaction was used to analyze the alleles of the GSTM1 and GSTT1 genes. As a result of this analysis was found a significant increase in the frequency of micronucleated cells in tick-borne encephalitis patients compared with the control group. In addition, the frequency of cytokinesis-blocked micronucleated T-lymphocytes was increased significantly higher than the one of micronucleated buccal cells. The most significant and prolonged increase in the frequency of micronucleated cells was associated with the mutant inactive variants of the genes GSTM1 (0/0) and GSTT1 (0/0). In the patients with burden the inactive forms of these genes, the cytogenetic instability of the cytokinesis-blocked blood T-lymphocytes could persist for up to six months. In case of buccal cells, the frequency of micronucleated cells was close to the one in the control group as early as 1-3 months after a course of treatment. Conclusion. It was found that the most increased and prolonged frequency of cytogenetically instable cells persisted in cytokinesis-blocked T-lymphocytes of peripheral blood of patients with tick-borne encephalitis who were carriers of the genotype with inactive variants of both GSTM1 (0/0) and GSTT1 (0/0 ) glutathione-S-transferase genes.

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ON BIOLOGY OF SOCKEYE SALMON ONCORHYNCHGUS NERKA FROM THE ZHUPANOVA RIVER (EAST KAMCHATKA)

Izvestiya TINRO ◽

10.26428/1606-9919-2018-193-78-87 ◽

2018 ◽

Vol 193 ◽

pp. 78-87

Author(s):

V. F. Bugaev ◽

I. V. Tiller

Keyword(s):

Body Length ◽

Sockeye Salmon ◽

Russian Far East ◽

Pink Salmon ◽

Far East ◽

Age Groups ◽

The Body ◽

The North ◽

Salmon Population ◽

The Mean

Runs and escapements of sockeye salmon to the Zhupanova River have increased since 1985 with the run of 10.63 . 103 ind. instead of 1.45 . 103 ind. in 1960–1984, on average. The increasing was reasoned by change of the pink salmon odd year-classes domination in West Kamchatka to domination of even year-classes after the extremely high escapement in 1983. In 2005–2006, the sockeye salmon stock in the Zhupanova River became even more higher that continues till nowadays (runs of 68.20 . 103 ind. in 2005–2017, on average). This growth corresponds with general increasing of the pacific salmons abundance in the Russian Far East as the result of favorable environmental and feeding conditions in the North Pacific. General biological indices (age, body length and weight, maturity, fecundity) of mature sockeye salmon originated from the Zhupanova River are presented on the data of commercial catches in the sea in 1999–2017. The sockeye salmon population from this river has 11 age groups. The age group 1.3 is the most abundant and associated with the age groups 1.2 and 1.4. Majority of sockeye in the catches from the Zhupanova has the age 1.3 (on average 66.2 % in 1999–2017). Besides, returns of underyearlings with age 0.2, 0.3, and 0.4, and the fish with age 2.2, 2.3, 2.4, 3.2, and 3.3 are detected. The body length and weight are similar for all age groups of sockeye salmon: for males/females the mean length is 57.56/57.70 cm, mean weight is 2.69/2.62 kg. The mean males:females ratio is 44.7 : 55.3. The mean absolute fecundity is 4121 eggs. In opposite of sockeye salmon in some rivers of East Kamchatka, the population of the Zhupanova has no negative year-to-year trend of the body length or weight.

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Formation Factors of Cytogenetic Violation of Rutilus rutilus (Cypriniformes, Cyprinidae) in Transformed River Ecosystems

Vestnik Zoologii ◽

10.2478/vzoo-2019-0038 ◽

2019 ◽

Vol 53 (5) ◽

pp. 423-432

Author(s):

M. O. Klymenko ◽

O. O. Biedunkova ◽

O. M. Klymenko ◽

A. M. Pryshchepa ◽

I. I. Statnik ◽

...

Keyword(s):

Peripheral Blood ◽

Structural Damage ◽

Age Groups ◽

Rutilus Rutilus ◽

Average Frequency ◽

The Body ◽

Small Rivers ◽

Hydrochemical Regime ◽

The North ◽

Copper Zinc

Abstract For the first time, an analysis of structural damage of the peripheral blood erythrocyte nucleus in Rutilus rutilus (Linnaeus, 1758) was carried out for the rivers of the north-western region of Ukraine. The effect of the hydrochemical regime on the formation of cytogenetic disturbances of the representatives of this species was also clarified. It was observed that during 2013–2017, the content of phosphates, suspended solids, fluorides, iron, copper, zinc, manganese, nitrite nitrogen and nitrate nitrogen in the surface waters of rivers most significantly exceeded the current quality standards. The average frequency of nuclear disorders of peripheral blood erythrocytes R. rutilus from the studied hydrosystems is 5.25 ± 0.29 ‰ (p < 0.01), with a noticeable excess of the level of spontaneous mutations in the older age groups of fish. In all analyzed samples of R. rutilus, among the total number of identified structural disorders of the nucleus, the largest share is made up of red blood cells from the micronucleus (from 40.0 to 62.0 %), which is evidence of cytogenetic disturbances in the body of fish. At the same time, disturbances of erythrocyte blood of roach from small rivers are on average 1.3 times (or 21.9 %) higher compared with individuals from medium-sized rivers. The formation of cytogenetic disturbances in R. rutilus under the conditions of the hydrochemical regime of rivers is described by a close (r = 0.99 at p < 0.003) multifactorial regression dependence. More important regressive coefficients of the dependency are acquired by such biogenes (NH4+, NO3−, PO4−) and toxicants (Cu2+, Zu2+, Mn2+, F2) in the background of oxygen regime peculiarities (COD, BOD5, O2).

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Mercury anomalies across the Palaeocene-Eocene Thermal Maximum

10.5194/cp-2018-121 ◽

2018 ◽

Cited By ~ 1

Author(s):

Morgan T. Jones ◽

Lawrence M. E. Percival ◽

Ella W. Stokke ◽

Joost Frieling ◽

Tamsin A. Mather ◽

...

Keyword(s):

Large Scale ◽

Recovery Period ◽

Volcanic Eruptions ◽

The Body ◽

The Arctic ◽

The North ◽

Carbon Isotope Excursion ◽

Thermal Maximum ◽

The North Sea ◽

Tephra Layers

Abstract. Large-scale magmatic events like the emplacement of the North Atlantic Igneous Province (NAIP) are often coincident with periods of extreme climate change such as the Palaeocene–Eocene Thermal Maximum (PETM). One proxy for volcanism in the geological record that is receiving increased attention is the use of mercury (Hg) anomalies. Volcanic eruptions are among the dominant natural sources of Hg to the environment; thus, elevated Hg/TOC values in the sedimentary rock record may reflect an increase in volcanic activity at the time of deposition. Here we focus on five continental shelf sections located around the NAIP in the Paleogene. We measured Hg, total organic carbon (TOC) concentrations, and δ13C values to assess how Hg deposition fluctuated across the carbon isotope excursion (CIE). We find a huge variation in Hg anomalies between sites. The Grane field in the North Sea, the most proximal locality to the NAIP analyzed, shows Hg concentrations up to 90,100 ppb (Hg/TOC = 95,700 ppb/wt%) in the early Eocene. Significant Hg/TOC anomalies are also present in Danish (up to 324 ppb/wt%) and Svalbard (up to 257 ppb/wt%) sections prior to the onset of the PETM and during the recovery period, while the Svalbard section also shows a continuous Hg/TOC anomaly during the body of the CIE. The combination with other tracers of volcanism, tephra layers and unradiogenic Os isotopes, at these localities suggests that the Hg/TOC anomalies reflect pulses of magmatic activity. In contrast, we do not observe clear Hg anomalies on the New Jersey shelf (Bass River) or the Arctic Ocean (Lomonosov Ridge). This large spatial variance could be due to more regional Hg deposition. One possibility is that phreatomagmatic eruptions and hydrothermal vent complexes formed during the emplacement of sills led to submarine Hg release, which is observed to result in limited distribution in the modern. The Hg/TOC anomalies in strata deposited prior to the CIE may suggest that magmatism linked to the emplacement of the NAIP contributed to the initiation of the PETM. However, evidence for considerable volcanism in the form of numerous tephra layers and Hg/TOC anomalies post-PETM indicates a complicated relationship between LIP volcanism and climate. Factors such as climate system feedbacks, changes to the NAIP emplacement style, and/or varying magma production rates may be key to both the onset and cessation of hyperthermal conditions during the PETM.

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The role of mutations in the genes FLG and GSTM1, and cytogenetic aberrations in the developmentof allergic occupational dermatitis in the oilfield workers of the north of Siberia

Rossiiskii Allergologicheskii ZHurnal ◽

10.36691/rja846 ◽

2011 ◽

Vol 8 (4) ◽

pp. 40-44

Author(s):

Nikolay Nikolaevich Il'inskikh ◽

B V Shilov ◽

I N Il'inskikh ◽

E N Il'inskikh ◽

A M Subbotin ◽

...

Keyword(s):

West Siberia ◽

Control Group ◽

Combined Effects ◽

Buccal Epithelium ◽

Occupational Dermatitis ◽

Chain Reaction ◽

The North ◽

Cytogenetic Aberrations ◽

Polymerase Chain

Background. The objective of the investigation was to determine frequencies of micronucleated cells in the buccal epithelium in relation to polymorphism of genes FLG and GSTM1 among oilfield workers suffered from allergic occupational dermatitis. Methods. There were 209 male oilfield workers examined with cases of allergic professional dermatitis at the age of 21-39 years old. as a control group there were used 207 male workers without allergic occupational dermatitis. we used genealogical method, micronucleous test and polymerase chain reaction to detect restriction fragments of genes GSTM1 and FLG. Results. it was detected that oilfield workers with genotypes contented homozygous mutated alleles GSTM1 (0/0) gene showed the higher frequency of micronucleated epithelial cells. it was not detected for FLG gene allele variants. among the examined oilfield workers with allergic occupational dermatitis there was nobody that would have both mutated alleles of FLG (282de14/282de14) and homozygous GSTM1 (0/0) genes, while in the control group we found some individuals of this type. Conclusions. The significantly higher levels of cytogenetic aberrations in the oilfield workers suffered from allergic occupational dermatitis are the result of combined effects of various factors on the genome. The investigation can provide the basis for the elaboration of new scientific criteria for hiring staff in oilfields of west siberia.

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Melatonin as an effective pharmacocorrector of alimentary obesity resulting from a long-term excessive intake of palm oil

Research Results in Pharmacology ◽

10.3897/rrpharmacology.4.25346 ◽

2018 ◽

Vol 4 (1) ◽

pp. 51-58

Author(s):

Elena Y. Bibik ◽

Natalia V. Shipilova ◽

Andrey V. Demenko

Keyword(s):

Body Weight ◽

Palm Oil ◽

Age Groups ◽

The Body ◽

Modern World ◽

Control Group ◽

Significant Alignment ◽

Daily Diet ◽

Alimentary Obesity ◽

Excessive Intake

Introduction: In the modern world, the problem of alimentary obesity is becoming one of the global problems affecting all countries. Desynchronosis of bodily rhythms is another possible cause of development of alimentary obesity and metabolic syndrome. Thus, the purpose of this research was to study the influence of melatonin on the dynamics of body weight in rats. Materials and Methods: The experiment was carried out on 150 white non-pedigree rats (females and males), which were divided into 3 groups (intact, control, experimental) and 3 series (immature, mature and senile rats). The animals of the control group received refined palm oil at a dose of 30 g/kg for a period of 6 weeks in their daily diet. The rats of the experimental group received melatonin at a dose of 1.9 mg/kg per day intragastrically for 35 days, after the 6-week period of excess palm oil intake was over. During the experiment, once every 7 days, the animals’ weight check was done, followed by assessing the dynamics of weight gain in rats in different age groups. Results and Discussion: It was determined that, in the group of the immature animals, an intake of palm oil resulted in the most obvious significant changes in the body weight over time. An increase in the total cholesterol and triglycerides concentration was observed in the biochemical blood tests of the animals of various age groups. Conclusion: The use of melatonin at a dose of 1.9 mg/kg per day for 35 days proved highly effective in the animals of all age groups and led to a significant alignment of changes in the biochemical parameters studied in the rats of all ontogenesis periods.

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Monitoring of cytogenetic effects in tick-borne encephalitis patients with type 2 diabetes mellitus, depending on polymorphism of glutathione-S-transferase genes

Diabetes Mellitus ◽

10.14341/dm9715 ◽

2019 ◽

Vol 22 (3) ◽

pp. 225-232

Author(s):

Nikolay N. Ilyinskikh ◽

Marina S. Kostromeeva ◽

Ekaterina N. Ilyinskikh

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Clinical Symptoms ◽

Micronucleus Assay ◽

Febrile Illness ◽

Buccal Cells ◽

Glutathione S Transferase ◽

Tick Borne Encephalitis

BACKGROUND: Tick-borne encephalitis (TBE) is an acute viral disease with activation of oxidative stress and increasing in cytogenetic instability. Clinical symptoms of infectious diseases usually more severe in patients with type 2 diabetes mellitus (DM2), especially in the case of burden in the genotype of mutant variants of glutathione-S-transferase genes GSTM1 and GSTT1. AIMS: The aim of this study was to study the dynamics of the frequency of micronucleated cells in patients with acute TBE with concomitant DM2, depending on the burden of active and inactive variants of glutathione-S-transferase genes (GSTM1 and GSTT1) in the patients genotype. MATERIALS AND METHODS: Totally, samples to make micronucleus assay were obtained from 138 patients with febrile illness of acute TBE, 64 of whom were diagnosed with concomitant DM2 (groups 3 and 4). As control groups, 57 healthy individuals (control 1) and 61 patients with DM2 (control 2) were examined. The samples of buccal cells for the micronucleus assay were repeatedly obtained from the individuals on the first day of admission, and also after 1 week, 1, 3 and 6 months. Polymerase chain reaction was used to analyze the variants of the GSTM1 and GSTT1 genes. RESULTS: On the first days of the disease, significant increases in the frequency of micronucleated buccal cells were determined in all TBE patients as compared to controls 1 and 2 (P0.001). Significant increases in the frequency of micronucleated buccal cells was revealed in groups 3 and 4 of the TBE patients who were carriers of inactive variants of the GSTM1(0) and GSTT1(0) genes, as compared to the subgroup of TBE patients with active variants of these genes (P0.001). In all subgroups of TBE patients with concomitant DM2, the frequencies of micronucleated cells were significantly higher than in the subgroups of TBE patients without DM2 (P0.001). Study of the dynamics of the frequency of micronucleated buccal cells, as compared to the control, demonstrated that the highest and long-lasting (within 6 months) cytogenetical effects were maintained in the group of TBE patients with genotype GSTM1(0)/GSTT1 (0) and concomitant DM2. CONCLUSION: The most prolonged and highest increases in the frequencies of cytogenetically instable cells were determined in the group of acute TBE patients with concomitant DM2 who were carriers of the genotype with inactive variants of both GSTM1(0) and GSTT1(0) glutathione-S-transferase genes.

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Mercury anomalies across the Palaeocene–Eocene Thermal Maximum

Climate of the Past ◽

10.5194/cp-15-217-2019 ◽

2019 ◽

Vol 15 (1) ◽

pp. 217-236 ◽

Cited By ~ 18

Author(s):

Morgan T. Jones ◽

Lawrence M. E. Percival ◽

Ella W. Stokke ◽

Joost Frieling ◽

Tamsin A. Mather ◽

...

Keyword(s):

Volcanic Activity ◽

Large Scale ◽

Recovery Period ◽

Volcanic Eruptions ◽

The Body ◽

The Arctic ◽

The North ◽

Carbon Isotope Excursion ◽

Thermal Maximum ◽

Tephra Layers

Abstract. Large-scale magmatic events like the emplacement of the North Atlantic Igneous Province (NAIP) are often coincident with periods of extreme climate change such as the Palaeocene–Eocene Thermal Maximum (PETM). One proxy for volcanism in the geological record that is receiving increased attention is the use of mercury (Hg) anomalies. Volcanic eruptions are among the dominant natural sources of Hg to the environment; thus, elevated Hg∕TOC values in the sedimentary rock record may reflect an increase in volcanic activity at the time of deposition. Here we focus on five continental shelf sections located around the NAIP in the Palaeogene. We measured Hg concentrations, total organic carbon (TOC) contents, and δ13C values to assess how Hg deposition fluctuated across the PETM carbon isotope excursion (CIE). We find a huge variation in Hg anomalies between sites. The Grane field in the North Sea, the most proximal locality to the NAIP analysed, shows Hg concentrations up to 90 100 ppb (Hg∕TOC = 95 700 ppb wt %−1) in the early Eocene. Significant Hg∕TOC anomalies are also present in Danish (up to 324 ppb wt %−1) and Svalbard (up to 257 ppb wt %−1) sections prior to the onset of the PETM and during the recovery period, while the Svalbard section also shows a continuous Hg∕TOC anomaly during the body of the CIE. The combination with other tracers of volcanism, such as tephra layers and unradiogenic Os isotopes, at these localities suggests that the Hg∕TOC anomalies reflect pulses of magmatic activity. In contrast, we do not observe clear Hg anomalies on the New Jersey shelf (Bass River) or the Arctic Ocean (Lomonosov Ridge). This large spatial variance could be due to more regional Hg deposition. One possibility is that phreatomagmatic eruptions and hydrothermal vent complexes formed during the emplacement of sills led to submarine Hg release, which is observed to result in limited distribution in the modern era. The Hg∕TOC anomalies in strata deposited prior to the CIE may suggest that magmatism linked to the emplacement of the NAIP contributed to the initiation of the PETM. However, evidence for considerable volcanism in the form of numerous tephra layers and Hg∕TOC anomalies post-PETM indicates a complicated relationship between LIP volcanism and climate. Factors such as climate system feedbacks, changes to the NAIP emplacement style, and/or varying magma production rates may be key to both the onset and cessation of hyperthermal conditions during the PETM. However, processes such as diagenesis and organic matter sourcing can have a marked impact on Hg∕TOC ratios and need to be better constrained before the relationship between Hg anomalies and volcanic activity can be considered irrefutable.

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Assessment of the quality of life of respondents working on a rotational basis in the Far North (on the example of the Khanty-Mansiysk Autonomous Okrug — Ugra)

Russian Osteopathic Journal ◽

10.32885/2220-0975-2021-2-66-75 ◽

2021 ◽

pp. 66-75

Author(s):

E. G. Ivanova ◽

E. V. Makarova

Keyword(s):

Quality Of Life ◽

Age Groups ◽

Health Indicators ◽

Climatic Conditions ◽

Point Of View ◽

The Body ◽

Far North ◽

The North ◽

Sf 36

Introduction. Currently, one of the directions in medicine is the study of the issue of the quality of life of patients. This indicator is a complex characteristic in terms of the physical and psychological component of health. The state of health is influenced by many factors and one of them is the peculiarities of the climatogeographic region where a person lives. The conditions of the Far North are characterized by extreme climatic and industrial conditions. In such areas, a rotational method is used, which assumes distance from the permanent place of residence. Thus, the influence of the climatic conditions of the Far North on the quality of life of respondents working on a rotational basis determines the relevance of this study, which was carried out in the Khanty-Mansi Autonomous Okrug — UGRA (KhMAO — Ugra).The aim of the research was to study the quality of life of respondents working on a rotational basis in the Far North.Materials and methods. Only men took part in this study — 207 people working in the conditions of the Far North (KhMAO — Ugra) on a rotational basis. All respondents were divided into four age groups: group 1 — 24–28 years old (n=36); group 2 — 29–34 years old (n=63); group 3 — 35–38 years old (n=78); group 4 — 39–45 years old (n=30). The duration of follow-up ranged from 1 to 3 months. A simple survey of all respondents was used to obtain information. In order to study the quality of life (QL) of those working in the Far North on a rotational basis, the MOS SF-36 questionnaire was used. Statistical analysis was performed using the STATGRAPHICS 12 Plus for Windows software in accordance with the general principles of QOL research in medicine.Results. With a long stay in the Far North, a restructuring of the adaptive mechanisms is noted, which goesthrough several stages: initially, minor physiological changes are noted that do not lead to serious changes in the body systems. The second stage is characterized by stable working capacity, but already there is a decrease in health indicators on all scales of the SF-36 questionnaire. The third stage or period of uncompensated fatigue is characterized by a significant decrease in psychological and physical indicators. Conclusion. Unfavorable from the point of view of the medico-biological aspect in the work on a rotational basis in the Far North is the coincidence of the period of work with the phase of the greatest adaptation falling on the first month of stay in the North.

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ANALISIS SITOGENETIK SEL EPITEL MUKOSA BUKAL PEKERJA STASIUN PENGISI BAHAN BAKAR UMUM DI KOTA YOGYAKARTA

Jurnal Teknosains ◽

10.22146/teknosains.43852 ◽

2021 ◽

Vol 10 (2) ◽

pp. 160

Author(s):

Regina TC. Tandelilin ◽

Elastria Widita ◽

Rini Maya Puspita ◽

Tang Sze Mun

Keyword(s):

Cross Sectional Study ◽

Control Group ◽

Buccal Cells ◽

Cross Sectional ◽

Cytogenetic Damage ◽

Petrol Station ◽

Carcinogenic Potential ◽

Wide Range ◽

Significant Difference ◽

Nuclear Alterations

Workers employed in petroleum station have a high-risk exposure to a wide range of toxic compounds with known mutagenic and carcinogenic potential. Cytogenetic damage might have happened if they continuously exposed to petroleum derivatives. This study aimed to analyse the cytogenetic damage in exfoliated buccal cells among petroleum station workers in Yogyakarta City. This cross-sectional study was carried out on 30 petrol station workers who are working at a different petrol station in Yogyakarta and the control group consisted of 30 healthy subjects. Examination for all subjects included frequencies of nuclear abnormalities, including pycnosis, karyorrhexis, and karyolysis. Cytological preparations were stained according to papanicolaou reaction and analyzed under light microscope for making a score for degenerative nuclear alterations (pycnosis, karyolysis and karyorrhexis). Analysis of buccal cells revealed that frequencies of pycnosis and karyorrhexis in petrol station workers were significantly higher than the control group (P<0.05). Conversely, there was no significant difference in karyolisis among groups. These findings indicate that the petrol station workers are under the risk of significant cytogenetic damage, particularly pycnosis and karyorrhexis.

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