PL - 023 Effects of Oleanolic Acid on Omega-3 and 6 Fatty Acids Metabolism in Rats fed with High Fructose Diet

Objective The present study investigated the effect of OA on metabolism of omega 3 and 6 together with genes associated with lipid metabolism in high fructose diet fed Sprague Dawley rats. Methods Real Time PCR qPCR was conducted to evaluate gene expressions levels. Gas Chromatography Mass Spectrometry (GS-MS): Lipid metabolism metabolites were assesed. Measurement of gene expression using qPCR analysis: Expressions levels of glucose transport and lipid metabolism genes were assessed. Measurements of inflammatory markers concentration in Blood plasma:TNF-α and adiponectin concentrations were determined. Results It was found that high fructose diet decreased gene ex pression levels of NRF-1 and GLUT-4 genes, however administration of OA increased expression levels of these genes. Furthermore, adiponectin concentration was lowered in HFD group however this was reversed when HFD was accompanied by OA administration. This increase in concentration of the anti-inflammatory molecule followed the increase in adiponectin receptor, AdipoR1 gene expression in skeletal muscle tissue. The results on lipid metabolism metabolites were consistent the observed molecular events, the anti-inflammatory signaling molecules (EPA and DHA) were five times higher in HF+OA than they were in HFD. High FAS,ACC-2 gene expression levels in HFD group, low 2 expression levels these genes together with increased CPT-1 in OA treated groups coincided with increased levels of arachidonic acid and linoleic acid levels in HFD, threefold increase compared to the control, and decreased levels in OA treated rats. Conclusions Supplementation of diet with oleanolic acid produces DHA and EPA, thus modulating anti-inflammatory response and modulating effects of exercise in reversing diabetes. We provided evidence to effect that diet has a therapeutic potential in managing metabolic syndrome related diseases.

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Long-Term Impact of Neonatal Intake of Oleanolic Acid on the Expression of AMP-Activated Protein Kinase, Adiponectin and Inflammatory Cytokines in Rats Fed with a High Fructose Diet

Nutrients ◽

10.3390/nu11020226 ◽

2019 ◽

Vol 11 (2) ◽

pp. 226 ◽

Cited By ~ 4

Author(s):

Mashudu Matumba ◽

Ademola Ayeleso ◽

Trevor Nyakudya ◽

Kennedy Erlwanger ◽

Novel Chegou ◽

...

Keyword(s):

Gene Expression ◽

Lipid Metabolism ◽

Oleanolic Acid ◽

Metabolic Diseases ◽

Adiponectin Concentration ◽

High Fructose Diet ◽

Glucose And Lipid Metabolism ◽

High Fructose ◽

Tnf Α

AMP-activated protein kinase (AMPK) is known to regulate both glucose and lipid metabolism, which play vital roles in the development of metabolic syndrome. One way of regulating AMPK is through hormonal activation using adiponectin. Patients diagnosed with type-2 diabetes (T2D) and obesity exhibit low adiponectin concentration levels in their blood. Moreover, studies have also shown that inflammatory processes play a significant role in the etiology of these metabolic diseases. In this study, the long-term effects of neonatal intake of oleanolic acid (OA) on the AMPK gene, genes associated with glucose transport and lipid metabolism, adiponectin levels, and inflammatory biomarkers in rats fed with a high fructose diet were investigated. Seven day old pups were randomly divided into five groups and treated as follows; 0.5% dimethylsulphoxide v/v in distilled water vehicle control (CON), oleanolic acid (OA, 60 mg/kg), high fructose diet (HF, 20% w/v), high fructose diet combined with oleanolic acid (HF+OA), and high fructose diet combined with metformin (HF+MET, 500 mg/kg). The treatments were administered once daily until day 14. The rats were then weaned at day 21 and fed standard rat chow and had ad libitum access to plain drinking water until day 112. The quantitative polymerase chain reaction (qPCR) was used to analyze the gene expressions of AMPK, Glut-4, Cpt-1, AdipoR1, AdipoR2, TNF-α, and IL-6 in the skeletal muscles. Bio-Plex Pro magnetic bead-based assay was used to measure plasma levels of inflammatory markers (TNF-α, IL-6, VEGF, and MCP-1) while ELISA kits were used to measure adiponectin concentration in blood plasma. The results obtained in this study showed that neonatal supplementation with OA significantly increased AMPK gene expression approximately ~4-fold in OA fed rats compared to those that were fed with HF alone. In addition, glut-4 gene expression was also significantly higher in the OA treatment group compared to all the other experimental groups except the CON group whereas Cpt-1 gene was more expressed when OA was administered alone. Together, these results indicated that OA can play a role in glucose and lipid metabolism gene regulation. Furthermore, the results showed that the OA group had ~1.5-fold increase in adiponectin concentration when comparedto the HF group. Moreover, HF increased levels of inflammatory cytokines, which was attenuated by neonatal administration of OA. Plasma concentration and gene expression in the skeletal muscle for TNF-α and IL-6 were significantly increased in rats that were treated with HF alone when compared to all the other groups. On the contrary, the high levels of TNF-α and IL-6 were reduced when OA was administered. These findings suggest that intake of oleanolic acid during the neonatal stage of development could be a potential strategic intervention for the long-term prevention of metabolic diseases such as T2D and obesity.

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Curcumin ameliorates protein expression changes involved in mitochondrial fatty acids metabolism in heart of mice fed a high-fructose diet

10.21203/rs.3.rs-17918/v1 ◽

2020 ◽

Author(s):

Cecilia Gabriela Meléndez-Salcido ◽

Katya Vargas-Ortiz ◽

Oscar Gerardo Silva-Gaona ◽

María Cristina León-García ◽

Luz Arcelia Ortega-Hernández ◽

...

Keyword(s):

Gene Expression ◽

Fatty Acids ◽

Beneficial Effect ◽

Therapeutic Potential ◽

Cardiometabolic Disease ◽

Control Group ◽

Standard Diet ◽

High Fructose Diet ◽

High Fructose ◽

Fatty Acids Metabolism

Abstract Background: It has been proposed that curcumin modulates the gene expression of different signaling pathways, improve the fatty acids metabolism and exerts a potential beneficial effect on cardiometabolic disease, but this has not been thoroughly demonstrated. In the present study, we evaluated the effect of curcumin upon the expression of PPARα, CPT1, MCAD, VLCAD and ACAA2 in heart of mice fed a high-fructose diet (HFD). Methods: Twenty-four mice C57BL/6 were divided into four groups (n=6) and treated for 15 weeks. Control group (C) received standard diet (SD), Curcumin group (C+Cur), Fructose group (F) and Fructose with Curcumin group (F+Cur). The groups were treated with 0.75% w/w curcumin mixed in the SD and 30% w/v fructose in water, respectively. Heart proteins expression were analyzed by Western Blot. Results: Curcumin supplementation increased PPARα and ACAA2 expression and decreased CPT1 and MCAD expression in heart of mice fed a HFD. However, it did not modify the VLCAD expression. Conclusions: Curcumin regulated PPARα, CPT1 and MCAD expression and increased ACAA2 expression; suggesting a therapeutic potential in the prevention of alterations in mitochondrial fatty acids metabolism in heart of mice fed a HFD.

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A Study on Neonatal Intake of Oleanolic Acid and Metformin in Rats (Rattus norvegicus) with Metabolic Dysfunction: Implications on Lipid Metabolism and Glucose Transport

Molecules ◽

10.3390/molecules23102528 ◽

2018 ◽

Vol 23 (10) ◽

pp. 2528 ◽

Cited By ~ 6

Author(s):

Mmahiine Molepo ◽

Ademola Ayeleso ◽

Trevor Nyakudya ◽

Kennedy Erlwanger ◽

Emmanuel Mukwevho

Keyword(s):

Fatty Acids ◽

Lipid Metabolism ◽

Oral Administration ◽

Free Fatty Acids ◽

Glucose Transport ◽

Oleanolic Acid ◽

Metabolic Dysfunction ◽

High Fructose Diet ◽

Glut 4 ◽

High Fructose

Metabolic syndrome, a cluster of different disorders which include diabetes, obesity and cardiovascular diseases, is a global epidemic that is growing at an alarming rate. The origins of disease can be traced back to early developmental stages of life. This has increased mortalities and continues to reduce life expectancies of individuals across the globe. The aim of this study was to investigate the sub-acute and long term effects of neonatal oral administration of oleanolic acid and metformin on lipids (free fatty acids, FFAs) and genes associated with lipid metabolism and glucose transport using a neonatal rat experimental model. In the first study, seven days old pups were randomly grouped into control—distilled water (DW); oleanolic acid (60 mg/kg), metformin (500 mg/kg), high fructose diet (20% w/v, HF), oleanolic acid (OA) + high fructose diet (OA + HF), and Metformin + high fructose diet (MET + HF) groups. The pups were treated for 7 days, and then terminated on postnatal day (PD) 14. In the second study, rat pups were initially treated similarly to study 1 and weaned onto normal rat chow and plain drinking water on PD 21 till they reached adulthood (PD112). Tissue and blood samples were collected for further analyses. Measurement of the levels of free fatty acids (FFAs) was done using gas chromatography-mass spectrometry. Quantitative polymerase chain reaction (qPCR) was used to analyze the gene expression of glut-4, glut-5, fas, acc-1, nrf-1 and cpt-1 in the skeletal muscle. The results showed that HF accelerated accumulation of saturated FFAs within skeletal muscles. The HF fed neonatal rats had increased stearic acid, which was associated with decreased glucose, suppressed expression of glut-4, glut-5, nrf-1 and cpt-1 genes, and increased expression of acc-1 (p < 0.01) and fas. OA + HF and MET + HF treated groups had increased mono- and polyunsaturated FFAs; oleic, and octadecadienoic acids than the HF group. These unsaturated FFAs were associated with increased glut-4, glut-5 and nrf-1 (p < 0.01) and decreased acc-1 and fas (p < 0.05) in both OA + HF and MET + HF treated groups. Conclusions: The present study shows that neonatal oral administration of oleanolic acid and metformin potentially protects against the development of fructose-induced metabolic dysfunction in the rats in both short and long time periods.

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Effect of Administration of Carnitine, Resveratrol, and Aromatic Amino Acids with High-Fat-High-Fructose Diet on Gene Expression in Liver of Rats: Full Transcriptome Analysis

Russian Journal of Genetics ◽

10.1134/s1022795421100136 ◽

2021 ◽

Vol 57 (10) ◽

pp. 1149-1163

Author(s):

N. V. Trusov ◽

S. A. Apryatin ◽

V. A. Shipelin ◽

A. A. Shumakova ◽

I. V. Gmoshinski ◽

...

Keyword(s):

Gene Expression ◽

Amino Acids ◽

Transcriptome Analysis ◽

Aromatic Amino Acids ◽

High Fat ◽

High Fructose Diet ◽

High Fructose

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Kombuchas from green and black teas reduce oxidative stress, liver steatosis and inflammation, and improve glucose metabolism in Wistar rats fed a high-fat high-fructose diet

Food & Function ◽

10.1039/d1fo02106k ◽

2021 ◽

Author(s):

Rodrigo Cardoso ◽

Luiza Dias Moreira ◽

Mirian Costa ◽

Renata Celi Lopes Toledo ◽

Mariana Grancieri ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Metabolism ◽

Glucose Metabolism ◽

Wistar Rats ◽

Liver Steatosis ◽

Black Tea ◽

High Fat ◽

High Fructose Diet ◽

High Fructose ◽

Green And Black Tea

The aim of this study was to evaluate the effect of green and black tea kombuchas consumption on adiposity, lipid metabolism, liver steatosis, oxidative stress, and inflammation in Wistar rats...

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