scholarly journals In Vitro Antioxidant Activity, Total Phenolic, Flavonoid and Tannin Contents in the Ajuga Bracteosa Wall. Ex Benth, Grown at Middle Hill Climatic Condition of Western Himalayas

2020 ◽  
Vol 5 (3) ◽  
pp. 198-203
Author(s):  
Anchala Guglani ◽  
H. K. Pandey ◽  
Rajeshwar K.K. Arya ◽  
Madhu Bala
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Western Himalayas ◽  
Plant Parts ◽  
Phytochemical Contents

The antioxidant activity of aqueous and alcoholic extracts of different plant parts viz, leaves, flower, stem and root of Ajuga bracteosa was investigated against various in-vitro antioxidant assays. The total phenolic, flavonoid and tannin contents also estimated. The results revealed the significant antioxidant potential and variation in the IC50, EC50 and phytochemical contents among all the plant parts. The aqueous extract of leaves exhibited significantly (P<0.05) highest antioxidant activity on 2,2-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acixd), 2,2-diphenyl-1-picryl hydrazyl, and potassium ferricyanide reducing power assay, with IC50 values of 0.2707±0.0008, 0.4409±0.0020, and EC50 value 0.3413±0.0030 mg/mL, respectively, followed by the other parts of the plant. The leaves extract also possess the highest total phenolics, flavonoid and tannin contents among all the parts. Similarly, the aqueous extract is better than the alcoholic extract of different parts as far as phytochemical contents and antioxidant activity concerned. The present study revealed that the aqueous extract of leaves had the highest antioxidant potential, which correlated with the high level of total phenolic fl flavonoid, and tannin contents. Therefore, higher the phytochemical contents, higher will be the antioxidant potential.

scholarly journals Exploration of Productivity, Chemical Composition, and Antioxidant Potential of Origanum vulgare L. Grown at Different Geographical Locations of Western Himalaya, India

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Shweta Goyal ◽  
Geeta Tewari ◽  
H. K Pandey ◽  
Anjali Kumari
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Reducing Power ◽  
Western Himalaya ◽  
Antioxidant Potential ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Origanum Vulgare ◽  
Field Station

Origanum vulgare L., the most complex and variable species of the genus Origanum, is used to cure various diseases in the traditional medicinal system. In the current investigation, O. vulgare was collected from its natural origin and further multiplied for two years at the experimental farm of DIBER field station, Auli, and afterwards, it was grown at three different altitudes, i.e., DIBER field station of Auli (2744 m), Pithoragarh (1524 m), and Haldwani (412 m), under natural field conditions. The essential oil was extracted using the Clevenger apparatus and analyzed by GC/MS and GC/FID. The total phenolic, flavonoid, and tannin content in the methanolic extracts of all the cultivations were estimated by using Folin–Ciocalteu reagent, aluminium chloride reagent, and Folin–Denis methods, respectively. The antioxidant activity of the essential oils and extracts of all the cultivations was also estimated by DPPH, ABTS, and reducing power methods. The highest amount of phenolics, flavonoids, and tannins was obtained in the Pithoragarh cultivation. Thymol was the major component in all the essential oils contributing 38.81% (Auli), 52.83% (Pithoragarh), and 31.86% (Haldwani) of the total oil. The highest antioxidant activity was exhibited by oregano oil of Pithoragarh cultivation, followed by Auli and Haldwani, respectively. The alcoholic extract of Pithoragarh cultivation had the highest antioxidant activity. The study infers that the plant demonstrated good results in terms of fresh weight, oil yield, antioxidant potential, and EO composition when grown at higher altitudes.

scholarly journals Evaluation of cholinesterase inhibitory and antioxidant potential of Wedelia chinensis: possible implications in alleviating Alzheimer’s disease

2021 ◽  
Author(s):  
Md. Aminul Islam ◽  
Shahed Zaman ◽  
Kushal Biswas ◽  
Md. Yusuf Al-Amin ◽  
Md. Kamrul Hasan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Active Compound ◽  
Inhibitory Activity ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Brain Lipid ◽  
Total Antioxidant

Abstract Background Wedelia chinensis has been reported as a folk medicine for the treatment of different diseases including neurodegenerative disease. Although the plant has been studied well for diverse biological activities, the effect of this plant in neurological disorder is largely unknown. The present study was undertaken to evaluate the cholinesterase inhibitory and antioxidant potential of W. chinensis. Methods The extract and fractions of the plant were evaluated for acetylcholinesterase and butyrylcholinesterase inhibitory activity by modified Ellman method. The antioxidant activity was assessed in several in vitro models/assays such as reducing power, total antioxidant capacity, total phenolic and flavonoid content, scavenging of 2,2’-diphenyl-1-picrylhydrazyl (DPPH) free radical and hydroxyl radical, and inhibition of brain lipid peroxidation. Chromatographic and spectroscopic methods were used to isolate and identify the active compound from the extract. Results Among the extract and functions, aqueous fraction (AQF) and ethylacetate fraction (EAF) exhibited high inhibition against acetylcholinesterase (IC50: 40.02 ± 0.164 µg/ml and 57.76 ± 0.370 µg/ml) and butyrylcholinesterase (IC50: 31.79 ± 0.182 µg/ml and 48.41 ± 0.053 µg/ml). Similarly, the EAF and AQF had high content of phenolics and flavonoids and possess strong antioxidant activity in several antioxidant assays including DPPH and hydroxyl radical scavenging, reducing power and total antioxidant activity. They effectively inhibited the peroxidation of brain lipid in vitro with IC50 values of 45.20 ± 0.995 µg/ml and 25.53 ± 0.042 µg/ml, respectively. A significant correlation was observed between total flavonoids and antioxidant and cholinesterase inhibitory activity. Activity guided chromatographic separation led to the isolation of a major active compound from the EAF and its structure was elucidated as apigenin by spectral analysis. Conclusions The potential ability of W. chinensis to inhibit the cholinesterase activity and peroxidation of lipids suggest that the plant might be useful for the management of AD.

scholarly journals PHYTOCHEMICALS DETECTION, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY STUDY ON BERRIES OF SOLANUM TORVUM

2018 ◽  
Vol 11 (11) ◽  
pp. 418
Author(s):  
Ida Christi Ve ◽  
Uma Poorani T ◽  
Nagarajaperumal G ◽  
Mohan S
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Quantitative Estimation ◽  
Reducing Power ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Solanum Torvum ◽  
Alcohol Extract ◽  
Pharmacologically Active

Objective: This present study is planned to reveal about the pharmacognostical details, phytochemistry, antimicrobial activity, and antioxidant activity of aqueous and alcoholic extract of the berries of Solanum torvum Swartz. belonging to the family Solanaceae, which is a very good anti-inflammatory medicine in traditional system of therapies.Methods: The physicochemical parameters such as ash values, extractive values, and total fiber content, qualitative preliminary phytochemical studies, quantitative estimation for total phenolic contents and flavonoid content, anjd inorganic minerals such as sodium, calcium, and potassium were estimated. Flavonoids present in the methanolic extract were isolated and its Rf values were calculated by high-performance thin-layer chromatography method and compared with that of the standard epicatechin. The antioxidant activity was evaluated by Reducing power ability, 1, 1-diphenyl-2-picrylhydrazyl method, and hydrogen peroxide methods. The antibacterial and antifungal activities of the aqueous and methanolic extracts are evaluated by disc diffusion method comparing the zone of inhibition with that of the standards such as amikacin and Ketoconazole.Results: It contains a number of potentially pharmacologically active chemicals including the sapogenin steroid, chlorogenin, alkaloids, flavonoids, phenols, and tannins. Both the extracts showed good activity, but the alcohol extract is more active toward all bacterial organisms and also toward fungi organism. Methanol extract shows good antioxidant activity. Antimicrobial activity and antioxidant activity of the berries are due to the presence of phytoconstituents present in it.Conclusion: These berries can be used for new formulation in nutraceuticals and polyherbal natural formulations for reducing the oxidative stress-related diseases and different therapies.

scholarly journals Antioxidant Activity and Total Phenolic Content in Shiitake Mycelial Exudates

2011 ◽  
Vol 6 (6) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Weihuan Huang ◽  
Joo-Shin Kim ◽  
Hau Yin Chung
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Shiitake Mushroom ◽  
Ferrous Ions

Exudates (DE) secreted from two shiitake mushroom mycelia (strains 1358 and L5458) were evaluated for their antioxidative properties and phenolic content. 1358DE and L5458DE showed distinct antioxidant activity in different in vitro assays, including scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, hydroxyl radical, superoxide anions and hydrogen peroxide; the ability to chelate ferrous ions; reducing power; hemolysis inhibition activity in rat erythrocyte; and lipid peroxidation inhibition (IC50 values of 1358DE and L5458DE were 3.3 and 132.6; 44.5 and > 1000; 26.9 and 53.7; 153.6 and >175.0; 176.0 and 521.0; 26.7 and 746.4; 47.8 and 736.9; and 3.1 and > 1000 μg/mL, respectively). Their total phenolic content was 237.33 and 24.08 mg gallic acid equivalent (GAE)/g of dry DE, respectively. Overall, these results show that 1358DE generally possesses better antioxidant properties than L5458DE, possibly due to its larger total phenolic content. Shiitake mushroom mycelial exudates, particularly of 1358DE, could be a good source of natural antioxidants.

scholarly journals In Vitro Antioxidant Activity and Phenolic Content of Cedrus brevifolia Bark

2014 ◽  
Vol 9 (4) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Elena Cretu ◽  
Juha-Pekka Salminen ◽  
Maarit Karonen ◽  
Anca Miron ◽  
Christiana Charalambous ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Phenolic Content ◽  
Reducing Power ◽  
Ethyl Acetate Fraction ◽  
In Vitro Assays ◽  
Total Phenolic ◽  
Highly Correlated ◽  
Antioxidant Effects

A raw extract and four extractive fractions were obtained from Cedrus brevifolia (Cyprus cedar) bark. They were all studied regarding the phenolic content and profile using spectrophotometry and HPLC-DAD-ESI-MS. The antioxidant activity was investigated using in vitro assays: DPPH and ABTS radicals scavenging and reducing power assays. The ethyl acetate fraction had the highest total phenolic and proanthocyanidin contents; a taxifolin-O-hexoside, catechin, epicatechin and procyanidin oligomers (three dimers, two trimers) were identified in this fraction. The ethyl acetate fraction was found to possess the highest DPPH and ABTS radicals scavenging effects (EC50=13.9 ± 0.3 and 2.3 ± 0.0 μg/mL, respectively) and reducing capacity (EC50=9.1 ±0.1 μg/mL). Antioxidant effects were highly correlated with total phenolic and proanthocyanidin contents (r=0.89-0.99). These results suggest that Cedrus brevifolia bark is a new source of antioxidants.

scholarly journals Evaluation of Free Radical Scavenging and Polyphenolic Contents of Bark of Pterygota alata Roxb

2014 ◽  
Vol 6 (3) ◽  
pp. 543-552 ◽  
Author(s):  
N. Jahan ◽  
M. S. Parvin ◽  
A. Khan ◽  
N. Das ◽  
M. S. Islam ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Soluble Fraction ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Deciduous Tree ◽  
Total Phenolic ◽  
Phenolic And Flavonoid

Pterygota alata is a large deciduous tree of Malvaceae family. The present study was designed to evaluate in vitro antioxidant activity of stem bark of the plant. Ferric reducing power (FRP) test, 1, 1- diphenyl-2-picryl hydrazyl (DPPH) free radical scavenging test and total antioxidant capacity (TAC) assay were used to detect the antioxidant activity. Total phenolic and flavonoid contents were also determined as they are well known phytochemicals with antioxidant property. The bark of the plant showed efficient reducing power as well as free radical scavenging property (IC50 values 52.25 - 172.05). The bark also found rich in total phenolic and flavonoid content. The highest amount of total phenolic content was found in chloroform soluble fraction (29.898 µg/mL) followed by ethyl acetate soluble fraction (15.88 µg/mL). The highest content of total flavonoid also detected in chloroform soluble fraction (107.56 µg/mL) but followed by crude ethanol extract (98.66 µg/mL). Overall, the bark of the plant possesses significant antioxidant activity, therefore can be used as a good natural source of antioxidant. © 2014 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v6i3.17586 J. Sci. Res. 6 (3), 543-552 (2014)

scholarly journals Studies on In Vitro Antioxidant Activity of Methanolic Extract and Fractions of Ficus Hispida Lin. Fruits

2016 ◽  
Vol 8 (3) ◽  
pp. 371-380 ◽  
Author(s):  
M. S. Hossain ◽  
S. Parvin ◽  
S. Dutta ◽  
M. S. I. Mahbub ◽  
M. E. Islam
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Traditional Use ◽  
Total Antioxidant ◽  
Ficus Hispida

The present study was designed to confirm the traditional use of the fruits of Ficus hispida Linn. (Moraceae) as an antioxidant agent. Fruits of the plant extracted with methanol and crude methanol extract (CME) were further fractionated with n-hexane, chloroform, and ethyl acetate. All the fractions, n-hexane (NHF), chloroform (CHF), ethyl acetate (EAF), aqueous (AQF) and CME were preliminary screened for in vitro antioxidant activity and total phenolic and total flavonoid content. In DPPH radical scavenging assay, CME exhibited highest scavenging activity (IC50 = 11.20 µg/mL) as compared to other fractions. In this assay, IC50 of reference standard BHT was 5.10 µg/mL. The reducing power of the samples was in the order as AQF > CME > CHF > EAF > NHF. The results for hydrogen peroxide scavenging activity indicated that CME, EAF and AQF had almost the same scavenging activity except NHF. Total antioxidant capacity of CME and other fractions were ranked as CHF > AQF > CME > EAF > NHF.  In the assay of antioxidant constituents (total phenol and total flavonoids content), the CME had highest phenolic and flavonoids content. The results indicate that Ficus hispida fruits could be considered as a potential source of natural antioxidant.

scholarly journals QUANTITATIVE STUDY OF TOTAL PHENOLIC AND ALKALOID CONTENT IN HYDROALCOHOLIC CONTENT IN RAUWOLFIA SERPENTINA

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Deepak Jain ◽  
Meenakshi Jain ◽  
Anurekha Jain
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Test Protocol ◽  
Rauwolfia Serpentina ◽  
Dpph Method

The objectives of this study are to screen the phytochemicals, estimate the content of flavonoid and alkaloids compounds and determine the antioxidant capacity of the Rauwolfia serpentina stem. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenol and alkaloids were determined by the well-known test protocol available in the literature. The hydro alcoholic extract of stem of Rauwolfia serpentina was studied for antioxidant activity on different in vitro models namely 1,1-diphenyl, 2-picryl hydrazyl (DPPH) method. Phytochemical analysis revealed the presence of phenols and flavonoids. The total flavonoids and alkaloids content of Rauwolfia serpentina stem of hydroalcoholic extract was 1.086 and 2.364mg/100mg respectively. Ascorbic acid used as standards was also evaluated for comparison. The extract showed dose dependent free radical scavenging property in the tested models. Rauwolfia serpentina stem extract showed IC50 value 68.10?g/ml for DPPH method, which was comparable to that of ascorbic acid (IC50=17.68?g/ml). The present study describes the phytochemical profile and antioxidant activity of Rauwolfia serpentina which will further used for medicinal applications.

scholarly journals Increased Phenolic Content and Enhanced Antioxidant Activity in Fermented Glutinous Rice Supplemented with Fu Brick Tea

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 671 ◽  
Author(s):  
Xiao Xu ◽  
Wenxiu Hu ◽  
Siduo Zhou ◽  
Chuanhai Tu ◽  
Xiudong Xia ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Hydroxyl Radical ◽  
Phenolic Content ◽  
Reducing Power ◽  
Total Phenolic ◽  
Food Ingredient ◽  
Antioxidant Power ◽  
Functional Activities ◽  
Glutinous Rice

Glutinous rice-based foods have a long history are consumed worldwide. They are also in great demand for the pursuit of novel sensory and natural health benefits. In this study, we developed a novel fermented glutinous rice product with the supplementation of Fu brick tea. Using in vitro antioxidant evaluation and phenolic compounds analysis, fermentation with Fu brick tea increased the total phenolic content and enhanced the antioxidant activity of glutinous rice, including scavenging of 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical, 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) radical, and hydroxyl radical, ferric-reducing antioxidant power, and ferric ion reducing power and iron chelating capability. Besides, compared with traditional fermented glutinous rice, this novel functional food exhibited a stronger activity for protecting DNA against hydroxyl radical-induced oxidation damage. Quantitative analysis by HPLC identified 14 compounds covering catechins and phenolic acids, which were considered to be positively related to the enhanced antioxidant capability. Furthermore, we found that 80% ethanol was a suitable extract solvent compared with water, because of its higher extraction efficiency and stronger functional activities. Our results suggested that this novel fermented glutinous rice could serve as a nutraceutical food/ingredient with special sensory and functional activities.

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