scholarly journals PHYTOCHEMICALS DETECTION, ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY STUDY ON BERRIES OF SOLANUM TORVUM

2018 ◽  
Vol 11 (11) ◽  
pp. 418
Author(s):  
Ida Christi Ve ◽  
Uma Poorani T ◽  
Nagarajaperumal G ◽  
Mohan S
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Quantitative Estimation ◽  
Reducing Power ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Solanum Torvum ◽  
Alcohol Extract ◽  
Pharmacologically Active

Objective: This present study is planned to reveal about the pharmacognostical details, phytochemistry, antimicrobial activity, and antioxidant activity of aqueous and alcoholic extract of the berries of Solanum torvum Swartz. belonging to the family Solanaceae, which is a very good anti-inflammatory medicine in traditional system of therapies.Methods: The physicochemical parameters such as ash values, extractive values, and total fiber content, qualitative preliminary phytochemical studies, quantitative estimation for total phenolic contents and flavonoid content, anjd inorganic minerals such as sodium, calcium, and potassium were estimated. Flavonoids present in the methanolic extract were isolated and its Rf values were calculated by high-performance thin-layer chromatography method and compared with that of the standard epicatechin. The antioxidant activity was evaluated by Reducing power ability, 1, 1-diphenyl-2-picrylhydrazyl method, and hydrogen peroxide methods. The antibacterial and antifungal activities of the aqueous and methanolic extracts are evaluated by disc diffusion method comparing the zone of inhibition with that of the standards such as amikacin and Ketoconazole.Results: It contains a number of potentially pharmacologically active chemicals including the sapogenin steroid, chlorogenin, alkaloids, flavonoids, phenols, and tannins. Both the extracts showed good activity, but the alcohol extract is more active toward all bacterial organisms and also toward fungi organism. Methanol extract shows good antioxidant activity. Antimicrobial activity and antioxidant activity of the berries are due to the presence of phytoconstituents present in it.Conclusion: These berries can be used for new formulation in nutraceuticals and polyherbal natural formulations for reducing the oxidative stress-related diseases and different therapies.

scholarly journals Phytochemical screening and in vitro antibacterial, antifungal, antioxidant and antitumor activities of the red propolis Alagoas

2019 ◽  
Vol 79 (3) ◽  
pp. 452-459 ◽  
Author(s):  
F. R. G. Silva ◽  
T. M. S. Matias ◽  
L. I. O. Souza ◽  
T. J. Matos-Rocha ◽  
S. A. Fonseca ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Gallic Acid ◽  
Bioactive Metabolites ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Dpph Method

Abstract The study aimed to evaluate the antimicrobial activity, antioxidant, toxicity and phytochemical screening of the Red Propolis Alagoas. Antimicrobial activity was evaluated by disk diffusion method. Determination of antioxidant activity was performed using the DPPH assay (1.1-diphenyl-2-picrylhydrazyl), FTC (ferric thiocyanate) and determination of phenolic compounds by Follin method. Toxicity was performed by the method of Artemia salina and cytotoxicity by MTT method. The phytochemical screening for the detection of allelochemicals was performed. The ethanol extract of propolis of Alagoas showed significant results for antimicrobial activity, and inhibitory activity for Staphylococcus aureus and Candida krusei. The antioxidant activity of the FTC method was 80% to 108.3% hydrogen peroxide kidnapping, the DPPH method showed an EC50 3.97 mg/mL, the content of total phenolic compounds was determined by calibration curve gallic acid, resulting from 0.0005 mg/100 g of gallic acid equivalent. The extract was non-toxic by A. salina method. The propolis extract showed high activity with a higher percentage than 75% inhibition of tumor cells OVCAR-8, SF-295 and HCT116. Chemical constituents were observed as flavonones, xanthones, flavonols, and Chalcones Auronas, Catechins and leucoanthocyanidins. It is concluded that the extract can be tested is considered a potential source of bioactive metabolites.

scholarly journals Exploration of Productivity, Chemical Composition, and Antioxidant Potential of Origanum vulgare L. Grown at Different Geographical Locations of Western Himalaya, India

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Shweta Goyal ◽  
Geeta Tewari ◽  
H. K Pandey ◽  
Anjali Kumari
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Reducing Power ◽  
Western Himalaya ◽  
Antioxidant Potential ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Origanum Vulgare ◽  
Field Station

Origanum vulgare L., the most complex and variable species of the genus Origanum, is used to cure various diseases in the traditional medicinal system. In the current investigation, O. vulgare was collected from its natural origin and further multiplied for two years at the experimental farm of DIBER field station, Auli, and afterwards, it was grown at three different altitudes, i.e., DIBER field station of Auli (2744 m), Pithoragarh (1524 m), and Haldwani (412 m), under natural field conditions. The essential oil was extracted using the Clevenger apparatus and analyzed by GC/MS and GC/FID. The total phenolic, flavonoid, and tannin content in the methanolic extracts of all the cultivations were estimated by using Folin–Ciocalteu reagent, aluminium chloride reagent, and Folin–Denis methods, respectively. The antioxidant activity of the essential oils and extracts of all the cultivations was also estimated by DPPH, ABTS, and reducing power methods. The highest amount of phenolics, flavonoids, and tannins was obtained in the Pithoragarh cultivation. Thymol was the major component in all the essential oils contributing 38.81% (Auli), 52.83% (Pithoragarh), and 31.86% (Haldwani) of the total oil. The highest antioxidant activity was exhibited by oregano oil of Pithoragarh cultivation, followed by Auli and Haldwani, respectively. The alcoholic extract of Pithoragarh cultivation had the highest antioxidant activity. The study infers that the plant demonstrated good results in terms of fresh weight, oil yield, antioxidant potential, and EO composition when grown at higher altitudes.

Xanthine Oxidase Inhibition, In Vitro Antioxidant Activity and Antibacterial Effect of the Aerial Part Extracts of Thymus pallidus Coss

2020 ◽  
Vol 16 (4) ◽  
pp. 607-615
Author(s):  
Abderrahim Benslama ◽  
Sabah Boumerfeg ◽  
Sana Aouachria ◽  
Thoraya Guemmaz ◽  
Lekhmici Arrar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Linoleic Acid ◽  
Aerial Part ◽  
Antibacterial Effect ◽  
Pharmaceutical Preparation ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Total Phenolic

Background: The Algerian medicinal plant Thymus pallidus Coss. is traditionally known to be used as an anti-infective, expectorant, healer of wounds and against inflammatory and gastrointestinal diseases. Objective: The aim of the present study is to investigate the antioxidants and the antibacterial activities of the aerial part extracts of this plant. Methods: Polyphenols extraction was performed with methanol 85%, followed by preliminary fractionation based on the polarity of the solvent. The extraction and fractionation process gave the crude extract (CrE), chloroform extract (ChE), ethyl acetate extract (EtAE) and aqueous extract (AqE). The antioxidant activity of extracts was evaluated using in vitro assays. While the antimicrobial activity was determined using disk diffusion method. Results: Quantitative analysis of polyphenol and flavonoids showed that EtAE includes the highest amount (P≤0.001) with 495.12±1.91 μg GAE/mg of extract and 97.25±2.84 μg QE/mg of extract, for both polyphenols and flavonoids respectively. The results of the antioxidant activity of extracts showed that the EtAE exhibited the highest inhibition activity on XO (IC50 =51.6±0.2 μg/ml) compared to other extracts (P≤0.001). Moreover, the EtAE was the most effective fraction in free radical scavenging assay against ABTS radical and hydrogen peroxide (EC50= 3.21±0.03 μg/ml and EC50 =12.12±0.05 μg/ml, respectively) compared to other extracts (P≤0.01). Using β-carotene/ linoleic acid bleaching assay, the AqE and ChE recorded a considerable inhibition effect on linoleic acid oxidation with a ratio of 81.81% and 80.86%, respectively. In the antimicrobial activity evaluation, the extracts exhibited moderate antibacterial activity against bacterial strain, with inhibition zone ranging from 07 to 14 mm. In addition, the EtAE showed a good action spectrum against six bacterial strains. In conclusion, the EtAE of Thymus pallidus showed good antioxidant activity and antibacterial effect. Conclusion: The obtained results revealed the presence of a strong correlation between the antioxidant and antibacterial activities of the extract and its total phenolic content. Furthermore, they reported that the EtAE has a considerable antioxidant capacity. This can be considered as an alternative natural source of antioxidants used generally as additives in food and pharmaceutical preparation.

scholarly journals Effects of Development and Ripening Stage on Phytochemical Compositions, Antioxidant and Antibacterial Activities of Date Palm Fruits

2019 ◽  
Vol 32 (2) ◽  
pp. 415-419
Author(s):  
F.Z. Bentebba ◽  
Z. Ghiaba ◽  
M. Saidi ◽  
C. Bensasi
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Disk Diffusion Method ◽  
Maturation Stages ◽  
Palm Fruits ◽  
Edible Parts

Edible parts of two dates cultivars (Deguel ahmar and Tanslit) at four maturation stages khellal, besser, rutab and tamr were analyzed for their phytochemical composition (total phenolic, total flavonoid and condensed tannins contents) as well as antioxidant and antibacterial activities. The antioxidant activity evaluated in vitro using scavenging assays of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and 2,2′-azino-bis(3- ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical and ferric reducing power. Antibacterial activity of extracts were evaluated in vitro by the agar disk diffusion method against five pathogenic bacterias trains: Bacillus subtilis ATCC 6633, Enterococcus ATCC 3315, Staphylococcus aureus ATCC 43300, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 7320. Results suggested that the content of these phytochemicals are very important at khellal stage and then decreased during maturation followed by a decrease in the antioxidant activity.

scholarly journals Antioxidant, Antimicrobial Activity and Toxicity Test ofPilea microphylla

2010 ◽  
Vol 2010 ◽  
pp. 1-6 ◽  
Author(s):  
Amir Modarresi Chahardehi ◽  
Darah Ibrahim ◽  
Shaida Fariza Sulaiman
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Toxicity Test ◽  
Radical Scavenging ◽  
Chloroform Extract ◽  
Total Flavonoid ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Total Phenolic Contents

A total of 9 plant extracts were tested, using two different kinds of extracting methods to evaluate the antioxidant and antimicrobial activities fromPilea microphylla(Urticaceae family) and including toxicity test. Antioxidant activity were tested by using DPPH free radical scavenging, also total phenolic contents and total flavonoid contents were determined. Toxicity assay carried out by using brine shrimps. Methanol extract of method I (ME I) showed the highest antioxidant activity at69.51±1.03. Chloroform extract of method I (CE I) showed the highest total phenolic contents at72.10±0.71and chloroform extract of method II (CE II) showed the highest total flavonoid contents at60.14±0.33. The antimicrobial activity ofPilea microphyllaextract was tested in vitro by using disc diffusion method and minimum inhibitory concentration (MIC). ThePilea microphyllaextract showed antibacterial activity against some Gram negative and positive bacteria. The extracts did not exhibit antifungal and antiyeast activity. The hexane extract of method I (HE I) was not toxic against brine shrimp (LC50 value was 3880 μg/ml). Therefore, the extracts could be suitable as antimicrobial and antioxidative agents in food industry.

scholarly journals Antioxidant and Antimicrobial Evaluation and Chemical Investigation of Rosa gallica var. aegyptiaca Leaf Extracts

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6498
Author(s):  
Ahmed S. Abdelbaky ◽  
Abir M. H. A. Mohamed ◽  
Salman S. Alharthi
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Leaf Extracts ◽  
Rosa Gallica

Rosa gallica var. aegyptiaca is a species of flowering plant belonging to the Rosaceae family that plays an important role as a therapeutic agent for the treatment of specific types of cancer, microbial infections, and diabetes mellitus. This work presents the first report on the evaluation of the antioxidant and antimicrobial potential along with the phytochemical analysis of Rosa gallica var. aegyptiaca leaves. Five leaf extracts of hexane, chloroform, methanol, hydromethanol 80%, and water were prepared. Assessment of antioxidant activity was carried out via DPPH radical scavenging assay. Antimicrobial activity against five foodborne pathogenic bacteria—including Listeria monocytogenes, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Salmonella enteritidis—and the fungus Candida albicans, was examined using the disc diffusion method. Total phenolic content and total flavonoid content were determined using the Folin–Ciocalteu reagent and aluminum chloride methods, respectively. Isolation, identification, and quantification of phenolic compounds were performed using HPLC-DAD analysis. Amongst the five leaf extracts that were investigated, hydromethanol 80% extract possessed the highest extraction yield, antioxidant activity, total phenolic content, and antimicrobial activity against all tested microbial strains. Moreover, this extract furnished six active phenolic compounds: gallic acid (1), (+) catechin (2), chlorogenic acid (3), (–) epicatechin (4), quercetin-3-O-α-d-(glucopyranoside) (5), and quercetin (6). This study provides an alternative utilization of R. gallica var. aegyptiaca leaves as a readily accessible source of natural antioxidants and antimicrobials in the food and pharmaceutical industries.

scholarly journals Antioxidant and antiproliferative activities of the n-butanol extract of Centaurea maroccana Ball aerial parts

2021 ◽  
Vol 34 (1) ◽  
pp. 5-11
Author(s):  
Imen Aissous ◽  
Mouad Benrebai ◽  
Ercan Cacan ◽  
Berkel Caglar ◽  
Ramazan Erenler ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Cell Lines ◽  
Proliferative Activity ◽  
Homo Sapiens ◽  
Quantitative Estimation ◽  
Reducing Power ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Phenolic Contents ◽  
Butanolic Extract

Abstract The aim of the present study is to evaluate the total phenolic contents, antioxidant and anti-proliferative activities of the n-BuOH extract of Centaurea maroccana (BECM). The total phenolic and flavonoid of the butanolic extract of the plant were assessed by using Folin-Ciocalteau and aluminium chloride colorimetric assays, respectively, and the quantitative estimation of total flavonoids and phenols revealed the richness of the extract in these compounds. Antioxidant activity was evaluated using standard lab colorimetric methods, while the anti-proliferative activity was evaluated using sulforhodamine B (SRB) assay against C6 (Rattus norvegicus brain glioma) and HT29 (Homo sapiens colorectal adenocarcinoma) cell lines. The n-butanolic extract of Centaurea maroccana showed a strong antioxidant activity through DPPH, ABTS•+, DMSO alkalin, Reducing power, β-Carotene-linoleic acid and CUPRAC assays. Furthermore, the anti-proliferative activity against C6 and HT29 of Centaurea maroccana exhibited a high effect by the decrease in viability of both cancer cell lines. Our results suggest a possible use of Centaurea maroccana as a source of natural antioxidant and chemo-preventive agents against cancer.

scholarly journals Evaluation of Phenolic Compounds and Antioxidant and Antimicrobial Activities of Some Common Herbs

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Muhammad Abdul Qadir ◽  
Syeda Kiran Shahzadi ◽  
Asad Bashir ◽  
Adil Munir ◽  
Shabnam Shahzad
Keyword(s):  
Antimicrobial Activity ◽  
Linoleic Acid ◽  
Reducing Power ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Bacterial Strains ◽  
Fungal Strains ◽  
Antioxidant And Antimicrobial Activities

The study was designed to evaluate the phenolic, flavonoid contents and antioxidant and antimicrobial activities of onion (Allium cepa), garlic (Allium sativum), mint (Mentha spicata), thyme (Thymus vulgaris), oak (Quercus), aloe vera (Aloe barbadensis Miller), and ginger (Zingiber officinale). All extracts showed a wide range of total phenolic contents, that is, 4.96 to 98.37 mg/100 g gallic acid equivalents, and total flavonoid contents, that is, 0.41 to 17.64 mg/100 g catechin equivalents. Antioxidant activity (AA) was determined by measuring reducing power, inhibition of peroxidation using linoleic acid system, and 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activity. Different extracts inhibited oxidation of linoleic acid by 16.6–84.2% while DPPH radical scavenging activity (IC50 values) ranged from 17.8% to 79.1 μg/mL. Reducing power at 10 mg/mL extract concentration ranged from 0.11 to 0.84 nm. Furthermore the extracts of these medicinal herbs in 80% methanol, 80% ethanol, 80% acetone, and 100% water were screened for antimicrobial activity by disc diffusion method against selected bacterial strains, Staphylococcus aureus, Escherichia coli, Bacillus subtilis, and Pasteurella multocida, and fungal strains, Aspergillus niger, Aspergillus flavus, Rhizopus solani, and Alternaria alternata. The extracts show better antimicrobial activity against bacterial strains as compared to fungal strains. Results of various assays were analyzed statistically by applying appropriate statistical methods.

scholarly journals Antimicrobial and antioxidant activity of the vegetative and reproductive organs of Robinia pseudoacacia

2014 ◽  
Vol 79 (11) ◽  
pp. 1363-1378 ◽  
Author(s):  
Ioana Marinas ◽  
Eliza Oprea ◽  
Elisabeta-Irina Geana ◽  
Carmen Chifiriuc ◽  
Veronica Lazar
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Robinia Pseudoacacia ◽  
Synergetic Effect ◽  
Reproductive Organs ◽  
Aureus Strain ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Ethanol Extracts ◽  
Antimicrobial And Antioxidant Activity

The study was aimed to investigate antimicrobial and antioxidant activity of ethanol extracts obtain from leaves, seeds and sheaths of R. pseudoacacia. Total phenolic content (TPC, Folin-Ciocalteu method), antioxidant activity (TEAC assay) and antimicrobial activity (agar disk diffusion method and broth dilution method) of vegetative and reproductive organs of R. pseudoacacia were done. The highest content of polyphenols (expressed as gallic acid equivalents, GAE) obtained for R. pseudoacacia leaves extract (266.7 ?g GAE mL-1 extract) followed by seeds extract (232.2 ?g GAE mL-1 extract). The HPLC analysis showed presence of catechin (0.925 ?g mL-1), rutin (0.831 ?g mL-1), resveratrol (0.664 ?g mL-1) and quercetin (0.456 ?g mL-1) in leaves and catechin (0.127 ?g mL-1), epicatechin (0.239 ?g mL-1) and rutin (0.231 ?g mL-1) in seeds extract. The results showed that the studied extracts exhibited a selective antimicrobial effect directed against Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Pseudomonas aeruginosa, Klebsiella pneumonie and Escherichia coli) bacterial strains. The combination leaves extract / antibiotic had the highest synergistic effect when compared to seeds and sheaths extracts. The same extract with penicillin, kanamycin and rifampin had highest synergetic effect against methicillin resistant S. aureus strain (MRSA), a strain which it has gained a great interest of microbiologists within past decades. The chemical characterization of ethanol extracts from the vegetative and reproductive organs of Robinia pseudoacacia, synergistic effects of certain antibiotics and acacia extracts, potential to increase antimicrobial activity of some commercial antibiotics against MRSA were done for the first time.

scholarly journals PRELIMINARY PHYTOCHEMICAL, ANTIOXIDANT AND ANTIMICROBIAL STUDIES OF FLACOURTIA JANGOMAS FRUITS

2017 ◽  
Vol 9 (4) ◽  
pp. 86 ◽  
Author(s):  
Sumit Das ◽  
Nuruzzaman Dewan ◽  
Kapil Joyti Das ◽  
Dhritishmita Kalita
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Reducing Power ◽  
Diffusion Method ◽  
Power Method ◽  
Disc Diffusion Method ◽  
Standard Drug ◽  
Antimicrobial Studies ◽  
In Vitro Antioxidant Activity

Objective: To determine phytochemical, antimicrobial and in vitro antioxidant activity of Flacourtia jangomas (Family: Flacourtiaceae)Methods: The fruit extracts of the plant was taken for the phytochemical screening to identify the phytochemicals present in it. The extracts of the fruits were evaluated for antimicrobial activity against S. aureus and Escherichia coli by disc diffusion method. In vitro, antioxidant activity was performed by reducing power method, TBA method.Results: The n-butanol extracts showed significant antimicrobial activity against all the strains as compared to the standard drug. The extracts also showed antioxidant activity against the standard ascorbic acid.Conclusion: The extracts showed a high amount of antimicrobial activity and antioxidant activity.

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