scholarly journals Fabricating spatially functionalized 3D-printed scaffolds for osteochondral tissue engineering

2021 ◽  
Vol 8 (1) ◽  
pp. e146
Author(s):  
Paula Camacho ◽  
Matthew Fainor ◽  
Kelly B. Seims ◽  
John W. Tolbert ◽  
Lesley W. Chow
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Spatial Organization ◽  
Three Dimensional ◽  
Stem Cell Differentiation ◽  
Human Mesenchymal Stem Cell ◽  
Layer By Layer ◽  
Matrix Deposition ◽  
3D Printed ◽  
Osteochondral Tissue

Three-dimensional (3D) printing of biodegradable polymers has rapidly become a popular approach to create scaffolds for tissue engineering. This technique enables fabrication of complex architectures and layer-by-layer spatial control of multiple components with high resolution. The resulting scaffolds can also present distinct chemical groups or bioactive cues on the surface to guide cell behavior. However, surface functionalization often includes one or more post-fabrication processing steps, which typically produce biomaterials with homogeneously distributed chemistries that fail to mimic the biochemical organization found in native tissues. As an alternative, our laboratory developed a novel method that combines solvent-cast 3D printing with peptide-polymer conjugates to spatially present multiple biochemical cues in a single scaffold without requiring post-fabrication modification. Here, we describe a detailed, stepwise protocol to fabricate peptide-functionalized scaffolds and characterize their physical architecture and biochemical spatial organization. We used these 3D-printed scaffolds to direct human mesenchymal stem cell differentiation and osteochondral tissue formation by controlling the spatial presentation of cartilage-promoting and bone-promoting peptides. This protocol also describes how to seed scaffolds and evaluate matrix deposition driven by peptide organization.

scholarly journals Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

2020 ◽  
Vol 6 (1) ◽  
pp. 57-69
Author(s):  
Amirhosein Fathi ◽  
Farzad Kermani ◽  
Aliasghar Behnamghader ◽  
Sara Banijamali ◽  
Masoud Mozafari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Composite Scaffolds ◽  
3D Printed ◽  
Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

scholarly journals Three-Dimensional Printing of Hydroxyapatite Composites for Biomedical Application

Crystals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 353
Author(s):  
Yanting Han ◽  
Qianqian Wei ◽  
Pengbo Chang ◽  
Kehui Hu ◽  
Oseweuba Valentine Okoro ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Biomedical Application ◽  
Three Dimensional ◽  
Antibacterial Properties ◽  
Natural Polymers ◽  
Three Dimensional Printing ◽  
3D Printed ◽  
Dental Applications ◽  
Hard Tissue Engineering

Hydroxyapatite (HA) and HA-based nanocomposites have been recognized as ideal biomaterials in hard tissue engineering because of their compositional similarity to bioapatite. However, the traditional HA-based nanocomposites fabrication techniques still limit the utilization of HA in bone, cartilage, dental, applications, and other fields. In recent years, three-dimensional (3D) printing has been shown to provide a fast, precise, controllable, and scalable fabrication approach for the synthesis of HA-based scaffolds. This review therefore explores available 3D printing technologies for the preparation of porous HA-based nanocomposites. In the present review, different 3D printed HA-based scaffolds composited with natural polymers and/or synthetic polymers are discussed. Furthermore, the desired properties of HA-based composites via 3D printing such as porosity, mechanical properties, biodegradability, and antibacterial properties are extensively explored. Lastly, the applications and the next generation of HA-based nanocomposites for tissue engineering are discussed.

scholarly journals Polymeric Bioinks for 3D Hepatic Printing

Chemistry ◽  
2021 ◽  
Vol 3 (1) ◽  
pp. 164-181
Author(s):  
Joyita Sarkar ◽  
Swapnil C. Kamble ◽  
Nilambari C. Kashikar
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Soft Tissues ◽  
Three Dimensional ◽  
Stem Cell Differentiation ◽  
Synthetic Polymers ◽  
Bioactive Molecules ◽  
Complex Geometries ◽  
Printing Techniques ◽  
Natural And Synthetic

Three-dimensional (3D) printing techniques have revolutionized the field of tissue engineering. This is especially favorable to construct intricate tissues such as liver, as 3D printing allows for the precise delivery of biomaterials, cells and bioactive molecules in complex geometries. Bioinks made of polymers, of both natural and synthetic origin, have been very beneficial to printing soft tissues such as liver. Using polymeric bioinks, 3D hepatic structures are printed with or without cells and biomolecules, and have been used for different tissue engineering applications. In this review, with the introduction to basic 3D printing techniques, we discuss different natural and synthetic polymers including decellularized matrices that have been employed for the 3D bioprinting of hepatic structures. Finally, we focus on recent advances in polymeric bioinks for 3D hepatic printing and their applications. The studies indicate that much work has been devoted to improvising the design, stability and longevity of the printed structures. Others focus on the printing of tissue engineered hepatic structures for applications in drug screening, regenerative medicine and disease models. More attention must now be diverted to developing personalized structures and stem cell differentiation to hepatic lineage.

scholarly journals 3D-Printed Biopolymers for Tissue Engineering Application

2014 ◽  
Vol 2014 ◽  
pp. 1-13 ◽  
Author(s):  
Xiaoming Li ◽  
Rongrong Cui ◽  
Lianwen Sun ◽  
Katerina E. Aifantis ◽  
Yubo Fan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Three Dimensional ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
Printing Technology ◽  
3D Printing Technology ◽  
Potential Applications ◽  
3D Printed ◽  
Dimensional Object

3D printing technology has recently gained substantial interest for potential applications in tissue engineering due to the ability of making a three-dimensional object of virtually any shape from a digital model. 3D-printed biopolymers, which combine the 3D printing technology and biopolymers, have shown great potential in tissue engineering applications and are receiving significant attention, which has resulted in the development of numerous research programs regarding the material systems which are available for 3D printing. This review focuses on recent advances in the development of biopolymer materials, including natural biopolymer-based materials and synthetic biopolymer-based materials prepared using 3D printing technology, and some future challenges and applications of this technology are discussed.

scholarly journals Parameters Affecting the Mechanical Properties of Three-Dimensional (3D) Printed Carbon Fiber-Reinforced Polylactide Composites

Polymers ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 2456
Author(s):  
Demei Lee ◽  
Guan-Yu Wu
Keyword(s):  
Mechanical Properties ◽  
Carbon Fiber ◽  
3D Printing ◽  
Three Dimensional ◽  
Fused Deposition Modelling ◽  
Layer By Layer ◽  
Fused Deposition ◽  
Bed Temperature ◽  
3D Printed ◽  
The Impact

Three-dimensional (3D) printing is a manufacturing technology which creates three-dimensional objects layer-by-layer or drop-by-drop with minimal material waste. Despite the fact that 3D printing is a versatile and adaptable process and has advantages in establishing complex and net-shaped structures over conventional manufacturing methods, the challenge remains in identifying the optimal parameters for the 3D printing process. This study investigated the influence of processing parameters on the mechanical properties of Fused Deposition Modelling (FDM)-printed carbon fiber-filled polylactide (CFR-PLA) composites by employing an orthogonal array model. After printing, the tensile and impact strengths of the printed composites were measured, and the effects of different parameters on these strengths were examined. The experimental results indicate that 3D-printed CFR-PLA showed a rougher surface morphology than virgin PLA. For the variables selected in this analysis, bed temperature was identified as the most influential parameter on the tensile strength of CFR-PLA-printed parts, while bed temperature and print orientation were the key parameters affecting the impact strengths of printed composites. The 45° orientation printed parts also showed superior mechanical strengths than the 90° printed parts.

Importance of 3D Printing Technology in Medical Fields

2019 ◽  
pp. 21-40
Author(s):  
Ranjit Barua ◽  
Sudipto Datta ◽  
Amit Roychowdhury ◽  
Pallab Datta
Keyword(s):  
Tissue Engineering ◽  
Additive Manufacturing ◽  
3D Printing ◽  
New Technology ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Medical Field ◽  
Printing Technology ◽  
3D Printing Technology ◽  
Proper Material

Three-dimensional or 3D printing technology is a growing interest in medical fields like tissue engineering, dental, drug delivery, prosthetics, and implants. It is also known as the additive manufacturing (AM) process because the objects are done by extruding or depositing the material layer by layer, and the material may be like biomaterials, plastics, living cells, or powder ceramics. Specially in the medical field, this new technology has importance rewards in contrast with conventional technologies, such as the capability to fabricate patient-explicit difficult components, desire scaffolds for tissue engineering, and proper material consumption. In this chapter, different types of additive manufacturing (AM) techniques are described that are applied in the medical field, especially in community health and precision medicine.

scholarly journals Printing 3D Hydrogel Structures Employing Low-Cost Stereolithography Technology

2020 ◽  
Vol 11 (1) ◽  
pp. 12 ◽  
Author(s):  
Leila Samara S. M. Magalhães ◽  
Francisco Eroni Paz Santos ◽  
Conceição de Maria Vaz Elias ◽  
Samson Afewerki ◽  
Gustavo F. Sousa ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Low Cost ◽  
Three Dimensional ◽  
Laser Source ◽  
Layer By Layer ◽  
Polyethylene Glycol Diacrylate ◽  
Glycol Diacrylate ◽  
Layer Structures ◽  
Complex Models ◽  
3D Printed

Stereolithography technology associated with the employment of photocrosslinkable, biocompatible, and bioactive hydrogels have been widely used. This method enables 3D microfabrication from images created by computer programs and allows researchers to design various complex models for tissue engineering applications. This study presents a simple and fast home-made stereolithography system developed to print layer-by-layer structures. Polyethylene glycol diacrylate (PEGDA) and gelatin methacryloyl (GelMA) hydrogels were employed as the photocrosslinkable polymers in various concentrations. Three-dimensional (3D) constructions were obtained by using the stereolithography technique assembled from a commercial projector, which emphasizes the low cost and efficiency of the technique. Lithium phenyl-2,4,6-trimethylbenzoyl phosphonate (LAP) was used as a photoinitiator, and a 404 nm laser source was used to promote the crosslinking. Three-dimensional and vascularized structures with more than 5 layers and resolutions between 42 and 83 µm were printed. The 3D printed complex structures highlight the potential of this low-cost stereolithography technique as a great tool in tissue engineering studies, as an alternative to bioprint miniaturized models, simulate vital and pathological functions, and even for analyzing the actions of drugs in the human body.

scholarly journals Recent advances in the development of nature-derived photocrosslinkable biomaterials for 3D printing in tissue engineering

2019 ◽  
Vol 23 (1) ◽  
Author(s):  
Geunho Choi ◽  
Hyung Joon Cha
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Uv Light ◽  
Three Dimensional ◽  
Main Body ◽  
Biological Functions ◽  
Non Invasive ◽  
Decellularized Extracellular Matrix ◽  
3D Printed ◽  
Control Light

Abstract Background In recent years, three-dimensional (3D) printing has begun to be widely used in tissue engineering. Natural biomaterials have been employed to overcome the limitations of synthetic polymers. However, their low mechanical strength and poor printability are major disadvantages. Photocrosslinking is the most promising fabrication strategy because it is non-invasive and easy to control light intensity and exposure. In this article, developments of photocrosslinkable natural biomaterials in the field of 3D printing are reviewed. Main body Photocrosslinkable biomaterials can be broadly classified into materials that use ultraviolet (UV) and visible lights. Many natural biomaterials such as gelatin, hydroxyapatite, silk fibroin, and pectin have been modified through acrylation, crosslinked by 365 nm UV light, and 3D printed. Riboflavin could also be used to crosslink and print collagen or decellularized extracellular matrix (dECM). In the case of silk-like aneroin and modified gelatin, crosslinking is possible by forming a dityrosine bond using 452 nm visible light. Conclusion Despite the tremendous researches on the developments of photocrosslinkable 3D printing natural biomaterials, further efforts are necessary to develop source biomaterials with excellent biological functions and sufficient mechanical integrity.

Importance of 3D Printing Technology in Medical Fields

2021 ◽  
pp. 704-717
Author(s):  
Ranjit Barua ◽  
Sudipto Datta ◽  
Amit Roychowdhury ◽  
Pallab Datta
Keyword(s):  
Tissue Engineering ◽  
Additive Manufacturing ◽  
3D Printing ◽  
New Technology ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Medical Field ◽  
Printing Technology ◽  
3D Printing Technology ◽  
Proper Material

Three-dimensional or 3D printing technology is a growing interest in medical fields like tissue engineering, dental, drug delivery, prosthetics, and implants. It is also known as the additive manufacturing (AM) process because the objects are done by extruding or depositing the material layer by layer, and the material may be like biomaterials, plastics, living cells, or powder ceramics. Specially in the medical field, this new technology has importance rewards in contrast with conventional technologies, such as the capability to fabricate patient-explicit difficult components, desire scaffolds for tissue engineering, and proper material consumption. In this chapter, different types of additive manufacturing (AM) techniques are described that are applied in the medical field, especially in community health and precision medicine.

scholarly journals Solvent-based Extrusion 3D Printing for the Fabrication of Tissue Engineering Scaffolds

2020 ◽  
Vol 6 (1) ◽  
pp. 19 ◽  
Author(s):  
Bin Zhang ◽  
Rodica Cristescu ◽  
Douglas B. Chrisey ◽  
Roger J. Narayan
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
New Technology ◽  
Three Dimensional ◽  
Tissue Engineering Scaffolds ◽  
Scaffold Fabrication ◽  
Simultaneous Control ◽  
Scaffold Structure ◽  
3D Printed ◽  
Natural Tissue

Three-dimensional (3D) printing has been emerging as a new technology for scaffold fabrication to overcome the problems associated with the undesirable microstructure associated with the use of traditional methods. Solvent-based extrusion (SBE) 3D printing is a popular 3D printing method, which enables incorporation of cells during the scaffold printing process. The scaffold can be customized by optimizing the scaffold structure, biomaterial, and cells to mimic the properties of natural tissue. However, several technical challenges prevent SBE 3D printing from translation to clinical use, such as the properties of current biomaterials, the difficulties associated with simultaneous control of multiple biomaterials and cells, and the scaffold-to-scaffold variability of current 3D printed scaffolds. In this review paper, a summary of SBE 3D printing for tissue engineering (TE) is provided. The influences of parameters such as ink biomaterials, ink rheological behavior, cross-linking mechanisms, and printing parameters on scaffold fabrication are considered. The printed scaffold structure, mechanical properties, degradation, and biocompatibility of the scaffolds are summarized. It is believed that a better understanding of the scaffold fabrication process and assessment methods can improve the functionality of SBE-manufactured 3D printed scaffolds.

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