Newer trends and techniques adopted for manufacturing of In vitro meat through "tissue-engineering" technology: A review

Artificial extracellular matrices (aECMs) are an extension of biomaterials that were developed as in-vitro model environments for tissue cells that mimic the native in vivo target tissues’ structure. This bibliometric analysis evaluated the research productivity regarding aECM based on tissue engineering technology. The Web of Science citation index was examined for articles published from 1990 through 2019 using three distinct aECM-related topic sets. Data were also visualized using network analyses (VOSviewer). Terms related to in-vitro, scaffolds, collagen, hydrogels, and differentiation were reoccurring in the aECM-related literature over time. Publications with terms related to a clinical direction (wound healing, stem cells, artificial skin, in-vivo, and bone regeneration) have steadily increased, as have the number of countries and institutions involved in the artificial extracellular matrix. As progress with 3D scaffolds continues to advance, it will become the most promising technology to provide a therapeutic option to repair or replace damaged tissue.

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Tissue Engineering in Stomatology: A Review of Potential Approaches for Oral Disease Treatments

Frontiers in Bioengineering and Biotechnology ◽

10.3389/fbioe.2021.662418 ◽

2021 ◽

Vol 9 ◽

Author(s):

Lilan Cao ◽

Huiying Su ◽

Mengying Si ◽

Jing Xu ◽

Xin Chang ◽

...

Keyword(s):

Tissue Engineering ◽

Bone Defects ◽

Clinical Applications ◽

Artificial Organs ◽

Oral Disease ◽

Engineering Technology ◽

New Approach ◽

Tissue Engineering Technology

Tissue engineering is an emerging discipline that combines engineering and life sciences. It can construct functional biological structures in vivo or in vitro to replace native tissues or organs and minimize serious shortages of donor organs during tissue and organ reconstruction or transplantation. Organ transplantation has achieved success by using the tissue-engineered heart, liver, kidney, and other artificial organs, and the emergence of tissue-engineered bone also provides a new approach for the healing of human bone defects. In recent years, tissue engineering technology has gradually become an important technical method for dentistry research, and its application in stomatology-related research has also obtained impressive achievements. The purpose of this review is to summarize the research advances of tissue engineering and its application in stomatology. These aspects include tooth, periodontal, dental implant, cleft palate, oral and maxillofacial skin or mucosa, and oral and maxillofacial bone tissue engineering. In addition, this article also summarizes the commonly used cells, scaffolds, and growth factors in stomatology and discusses the limitations of tissue engineering in stomatology from the perspective of cells, scaffolds, and clinical applications.

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Smurf1-targeting miR-19b-3p-modified BMSCs combined PLLA composite scaffold to enhance osteogenic activity and treat critical-sized bone defects

Biomaterials Science ◽

10.1039/d0bm01251c ◽

2020 ◽

Vol 8 (21) ◽

pp. 6069-6081

Author(s):

Ao Xiong ◽

Yijun He ◽

Liang Gao ◽

Guoqing Li ◽

Jian Weng ◽

...

Keyword(s):

Tissue Engineering ◽

Composite Scaffold ◽

Biological Materials ◽

Bone Defects ◽

Engineering Technology ◽

Osteogenic Activity ◽

The Past ◽

Seed Cells ◽

Tissue Engineering Technology

Over the past few years, tissue-engineering technology provided a new direction for bone defects therapy, which involved developing applicable biological materials composite with seed cells to repair bone defects tissue.

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DEVELOPMENTAL STATUS AND PERSPECTIVES FOR TISSUE ENGINEERING IN UROLOGY

NATURE AND SCIENCE ◽

10.36719/2707-1146/12/5-13 ◽

2021 ◽

Vol 12 (07) ◽

pp. 5-13

Author(s):

Elcin Nizami Huseyn ◽

Keyword(s):

Tissue Engineering ◽

Biological Materials ◽

Organ Damage ◽

Biological Tissues ◽

Tissue Cell ◽

Sperm Cells ◽

Engineering Technology ◽

The Future ◽

Tissue Engineering Technology

Tissue engineering technology and tissue cell-based stem cell research have made great strides in treating tissue and organ damage, correcting tissue and organ dysfunction, and reducing surgical complications. In the past, traditional methods have used biological substitutes for tissue repair materials, while tissue engineering technology has focused on merging sperm cells with biological materials to form biological tissues with the same structure and function as their own tissues. The advantage is that tissue engineering technology can overcome donors. Material procurement restrictions can effectively reduce complications. The aim of studying tissue engineering technology is to find sperm cells and suitable biological materials to replace the original biological functions of tissues and to establish a suitable in vivo microenvironment. This article mainly describes the current developments of tissue engineering in various fields of urology and discusses the future trends of tissue engineering technology in the treatment of complex diseases of the urinary system. The results of the research in this article indicate that while the current clinical studies are relatively few, the good results from existing animal model studies indicate good prospects of tissue engineering technology for the treatment of various urinary tract diseases in the future. Key words: Tissue engineering, kidney, ureter, bladder, urethra

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Cerebrospinal Fluid Pulsation Stress Promotes the Angiogenesis of Tissue-Engineered Laminae

Stem Cells International ◽

10.1155/2020/8026362 ◽

2020 ◽

Vol 2020 ◽

pp. 1-12

Author(s):

Linli Li ◽

Yiqun He ◽

Han Tang ◽

Wei Mao ◽

Haofei Ni ◽

...

Keyword(s):

Tissue Engineering ◽

Cerebrospinal Fluid ◽

Engineering Technology ◽

Expression Levels ◽

Fluid Pulsation ◽

Endothelial Growth Factor ◽

The Impact

Background. Angiogenesis is a prerequisite step to achieve the success of bone regeneration by tissue engineering technology. Previous studies have shown the role of cerebrospinal fluid pulsation (CSFP) stress in the reconstruction of tissue-engineered laminae. In this study, we investigated the role of CSFP stress in the angiogenesis of tissue-engineered laminae. Methods. For the in vitro study, a CSFP bioreactor was used to investigate the impact of CSFP stress on the osteogenic mesenchymal stem cells (MSCs). For the in vivo study, forty-eight New Zealand rabbits were randomly divided into the CSFP group and the Non-CSFP group. Tissue-engineered laminae (TEL) was made by hydroxyapatite-collagen I scaffold and osteogenic MSCs and then implanted into the lamina defect in the two groups. The angiogenic and osteogenic abilities of newborn laminae were examined with histological staining, qRT-PCR, and radiological analysis. Results. The in vitro study showed that CSFP stress could promote the vascular endothelial growth factor A (VEGF-A) expression levels of osteogenic MSCs. In the animal study, the expression levels of angiogenic markers in the CSFP group were higher than those in the Non-CSFP group; moreover, in the CSFP group, their expression levels on the dura mater surface, which are closer to the CSFP stress stimulation, were also higher than those on the paraspinal muscle surface. The expression levels of osteogenic markers in the CSFP group were also higher than those in the Non-CSFP group. Conclusion. CSFP stress could promote the angiogenic ability of osteogenic MSCs and thus promote the angiogenesis of tissue-engineered laminae. The pretreatment of osteogenic MSC with a CSFP bioreactor may have important implications for vertebral lamina reconstruction with a tissue engineering technique.

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Biofunctionalization of decellularized porcine aortic valve with OPG-loaded PCL nanoparticles for anti-calcification

RSC Advances ◽

10.1039/c9ra00408d ◽

2019 ◽

Vol 9 (21) ◽

pp. 11882-11893

Author(s):

Yang Li ◽

Yu Zhang ◽

Jing-Li Ding ◽

Ji-Chun Liu ◽

Jian-Jun Xu ◽

...

Keyword(s):

Tissue Engineering ◽

Controlled Release ◽

Aortic Valve ◽

Drug Loading ◽

Biological Factor ◽

Engineering Technology ◽

Loading System ◽

Tissue Engineering Technology

A novel composite valve with controlled release OPG was prepared by introducing tissue engineering technology and nano drug-loading system to introduce anti-calcification biological factor OPG on the decellularized valve.

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Production and characterization of an in vitro engineered human oral mucosa

Biochemistry and Cell Biology ◽

10.1139/o01-237 ◽

2002 ◽

Vol 80 (2) ◽

pp. 189-195 ◽

Cited By ~ 38

Author(s):

Mahmoud Rouabhia ◽

Noëlla Deslauriers

Keyword(s):

Tissue Engineering ◽

Epithelial Cells ◽

Oral Mucosa ◽

Oral Diseases ◽

Ki 67 ◽

Necrosis Factor Alpha ◽

Engineering Technology ◽

Cell Monolayers ◽

Factor Alpha

The role of epithelial cells in oral pathologies is poorly understood. Until now, most studies have used normal or transformed epithelial cell monolayers, a system that largely bypasses oral mucosal complexity. To overcome these limitations, an engineered human oral mucosa (EHOM) model has been produced and characterized. Following histological and immunohistochemical analyses, EHOM showed well-organized and stratified tissues in which epithelial cells expressed proliferating keratins such as Ki-67, K14, and K19 and also differentiating keratin (K10). In this model, epithelial cells interacted with fibroblasts in the lamina propria by secreting basement membrane proteins (laminins) and by expressing integrins (β1 and α2β1). Cytokine analyses using cultured supernatants showed that cells in EHOM were able to secrete interleukins (IL) including IL-1β and IL-8 and tumor necrosis factor alpha (TNF-α). Finally, cells in this engineered model were able to secrete different metalloproteinases such as gelatinase-A and gelatinase-B. In conclusion, using tissue engineering technology, we produced well-organized EHOM tissues. It is anticipated that this model will be useful for examining mechanisms involved in oral diseases under controlled conditions by modeling the interactions between mucosa and microorganisms in the oral cavity.Key words: tissue engineering, oral mucosa, periodontitis, keratinocytes, fibroblasts.

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