In Vitro Screening of Antibacterial Properties of Rhus coriaria and Origanum vulgare against Some Pathogenic Bacteria

This study aimed to characterize the in vitro antioxidant and antibacterial properties of oregano (Origanum vulgare) essential oil, as well as its chemical composition. To our best knowledge, there are few studies on oregano grown in the arid Andes region, but none on the metabolites produced and their bioactivity. This work identified fifty metabolites by Gas Chromatography–Mass Spectrometry (GC-MS)—monoterpene hydrocarbons, oxygenated monoterpenes, phenolic monoterpenes, sesquiterpene hydrocarbons, and oxygenated sesquiterpenes—present in the essential oil of oregano collected in the Atacama Desert. The main components of essential oregano oil were thymol (15.9%), Z-sabinene hydrate (13.4%), γ-terpinene (10.6%), p-cymene (8.6%), linalyl acetate (7.2%), sabinene (6.5%), and carvacrol methyl ether (5.6%). The antibacterial tests showed that the pathogenic bacteria Staphylococcus aureus and Salmonella enterica and the phytopathogenic bacteria Erwinia rhapontici and Xanthomonas campestris were the most susceptible to oregano oil, with the lowest concentrations of oil necessary to inhibit their bacterial growth. Moreover, oregano oil showed antibacterial activity against bacteria associated with food poisoning. In conclusion, O. vulgare from the arid Andean region possesses an important antibacterial activity with a high potential in the food industry and agriculture.

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Phytochemical composition and in vitro screening of the antimicrobial activity of essential oils on oral pathogenic bacteria

Natural Product Research ◽

10.1080/14786419.2017.1329730 ◽

2017 ◽

Vol 32 (5) ◽

pp. 544-551 ◽

Cited By ~ 27

Author(s):

Roberta Tardugno ◽

Federica Pellati ◽

Ramona Iseppi ◽

Moreno Bondi ◽

Giacomo Bruzzesi ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Pathogenic Bacteria ◽

In Vitro Screening ◽

Phytochemical Composition

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The characteristics of lactic acid bacteria isolated from fermented food as potential probiotics

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2020-0482 ◽

2021 ◽

Vol 32 (4) ◽

pp. 743-749

Author(s):

Victoria Yulita Fitriani ◽

Budi Suprapti ◽

Muhammad Amin

Keyword(s):

Lactobacillus Acidophilus ◽

Bile Salts ◽

Pathogenic Bacteria ◽

Fruit Juice ◽

Lactobacillus Reuteri ◽

Antibacterial Properties ◽

Fermented Food ◽

Cow’S Milk ◽

Cow's Milk

Abstract Objectives This study aims to determine the characteristics of Lactobacillus acidophilus and Lactobacillus reuteri from fermented soursop fruit juice and cow’s milk, respectively as probiotic candidate based on exposure to pH, bile salts, pathogenic bacteria, and antibiotics. Methods In vitro studies were conducted to examine the resistance of Lactobacillus acidophilus and Lactobacillus reuteri in pH 2, 2.5, 3.2, and 7.2, resistance to bile salts, resistance to pathogenic bacteria (Escherichia coli, Staphylococcus aureus and Enterococcus faecalis) and antituberculosis antibiotics. Results Viability of Lactobacillus acidophilus and Lactobacillus reuteri isolates remained unchanged (6.3 × 107 CFU/mL and 5.03 × 107 CFU/mL) at various acidic pH, and had a low survival rate in Ox gall 0.3% (bile salts). These isolates also showed antibacterial properties against pathogens in the gastrointestinal tract. Both of these bacteria are quite safe to be used together with ofloxacin, linezolid, moxifloxacin, and levofloxacin, antibiotic for tuberculosis therapy. Conclusions The results showed that Lactobacillus acidophilus and Lactobacillus reuteri from fermented soursop fruit juice and cow’s milk respectively fulfilled the characteristics of probiotic and could potentially be used as adjunct therapy in tuberculosis drug-resistance.

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Application of Magnetic Nanoparticles with Structure of Core-shell, as Bactericidal

Immunology and Inflammation Diseases Therapy ◽

10.31579/2637-8876/023 ◽

2018 ◽

Vol 2 (1) ◽

pp. 01-04

Author(s):

Mansour Binandeh

Keyword(s):

Magnetic Nanoparticles ◽

Pathogenic Bacteria ◽

Antibacterial Properties ◽

Correlation Method ◽

Core Shell ◽

Ft Ir ◽

Chemical Correlation ◽

Ir Analysis ◽

The Magnetic Field

Initially, magnetic nanoparticles (MNP) Fe3O4 are synthesized by a chemical correlation method and its core / shell structure is detected using SEM, FT-IR analysis. The purpose of this production was to use the nanoparticle performance level in the absorption of antibiotics, namely, ampicillin (amp). Absorption sampling was analyzed by UV-Vis spectrophotometer and the results indicate that the absorbance of the ampere increases to 85%. The bond between these two is electrostatic bonding, which was confirmed by EDX analysis. Ultimately, this compound was used for the antibacterial process. In this case, the MNP-amp compound was added in a natural amount of 20 μl a bacterial culture pattern overnight (In-vitro). The results showed that 95% of the bacteria were killed (confirmation of antibacterial properties of MNP). Therefore, it can be transmitted intentionally by controlling the magnetic field into living cells for the destruction of pathogenic bacteria.

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Antibacterial properties of rain tree ( ) and Mexican sunflower (Tithonia diversifolia ) used as fodder in ruminant nutrition

Nigerian Journal of Animal Production ◽

10.51791/njap.v45i2.528 ◽

2020 ◽

Vol 45 (2) ◽

pp. 331-341

Author(s):

F. T. Ajayi ◽

S. O. Omotoso ◽

O. J. Babayemi

Keyword(s):

Foodborne Pathogens ◽

Pathogenic Bacteria ◽

Antibacterial Properties ◽

Tithonia Diversifolia ◽

Standard Procedures ◽

Fodder Plants ◽

Zones Of Inhibition ◽

Ethanol Extracts ◽

Albizia Saman

Acetone and ethanol extracts of Albizia saman (ASL) and Tithonia diversifolia (TDL) leaves used as fodder for ruminant were evaluated for their antibacterial properties against selected pathogenic bacteria. Phytochemical screening was determined according to standard procedures, while antibacterial activity was by agar well diffusion and broth micro dilution methods. The levels of tannin (0.29 and 0.34 mg/100g); saponin (0.75 and 0.59 mg/100g); oxalates (0.17 and 0.14mg/100g); and phytate (0.11 and 0.12 mg/100g) in the fodder plants were below critical levels that may affect digestibility in ruminants. Acetone and ethanol extracts from ASL and TDL showed maximum zones of inhibition against Staphylococcus aureus (19.00 and 14.50 mm; 23.00 and 21.50 mm) and Pseudomonas aeruginosa (15.50 and 12.00 mm; 18.50 and 17.00 mm), respectively compared to Gentamycin (9.97 mm). Minimum inhibitory concentration (MIC) values of acetone and ethanol extracts from ASL ranged from 0.10 to 0.28 mg/mL and 0.13 to 0.22 mg/mL, while TDL extracts ranged from 0.20 to 0.32 mg/mL and 0.20 to 0.31 mg/mL, respectively for all the tested organisms. Minimum bactericidal concentration (MBC) values of ethanol extracts from both plant ranged from 0.5-0.80 mg/mL and 0.7-0.90 mg/mL compared to MBC values (0.02-0.04 mg/mL) of the reference antibiotic (gentamycin) for all the tested bacteria species. In conclusion, antibacterial properties exhibited by the plant extract implied that the bioactive compounds are potential antibacterial agents against pathogenic bacteria of ruminant or foodborne pathogens in vitro.

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The Application of a Recombinant Antimicrobial Peptide of Thrombocidin-1 expressed in Pichia pastoris as a Novel Approach Against Some Oral Pathogenic Bacteria: An In-vitro Study

Protein and Peptide Letters ◽

10.2174/0929866528666211126161928 ◽

2021 ◽

Vol 28 ◽

Author(s):

Fatemeh Forouzanfar ◽

Hamideh Sadat Mohammadipour ◽

Majid Akbari ◽

Reza Beyraghshamshir ◽

Abbas Tanhaeian ◽

...

Keyword(s):

Pichia Pastoris ◽

Pathogenic Bacteria ◽

Antibacterial Properties ◽

Dilution Method ◽

Human Gingival Fibroblast ◽

Gingival Fibroblast ◽

Fibroblast Cells ◽

Oral Infections ◽

Oral Pathogens

Objective: Oral infections and dental caries are considered serious health problems. Therefore, searching for new agents with antimicrobial properties seems to be crucial. This study aimed to evaluate the antimicrobial activity of the recombinant Thrombocidin-1 [TC-1] peptide on some oral pathogens. Also, the cytotoxicity of this peptide on human gingival fibroblast cells was investigated. Methods & Materials: In this study, Pichia pastoris was used for the expression of recombinant TC-1. The microbroth dilution method was used to determine the minimum inhibitory concentration [MIC] and minimum bacterial concentration [MBC]. It tested against four main oral pathogens; Streptococcus mutans, Streptococcus salivarius, Streptococcus oralis, and Enterococcus faecalis. Moreover, the cytotoxicity analysis was done on gingival fibroblast cells by the MTT method. The data were analyzed using a two-way analysis of variance [ANOVA] and Tukey’s HSD tests. Results: The most bactericidal effect of TC-1 was against S. salivarius, the highest bacteriostatic effect was against S. salivarius, and S. oralis had the lowest MIC value of 1.512 μg/ml. The Thrombocidin-1 peptide showed lower antibacterial properties against E. faecalis compared with CHX, unlike the stronger antimicrobial effect on examined streptococci. According to cytotoxicity examination, no concentration of TC-1 presented over 50% growth inhibition [IC50] of the fibroblasts cells. Conclusion: Based on antimicrobial tests and cytotoxicity results, the Thrombocidin-1 peptide may be useful as a safe antibacterial agent against some oral pathogens in dental materials.

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Toluidine blue O and porphyrin-mediated photodynamic therapy on three main pathogenic bacteria of periodontitis using portable LED phototherapy device

Journal of Innovative Optical Health Sciences ◽

10.1142/s1793545815500170 ◽

2015 ◽

Vol 08 (04) ◽

pp. 1550017 ◽

Cited By ~ 3

Author(s):

Xuewei Jiang ◽

Zhichao Fan ◽

Yili Yu ◽

Chenying Shao ◽

Yuanzhen Suo ◽

...

Keyword(s):

Photodynamic Therapy ◽

Toluidine Blue ◽

Pathogenic Bacteria ◽

Light Emitting Diode ◽

Red Light ◽

In Vitro Screening ◽

Light Emitting ◽

Led Phototherapy ◽

Prevotella Melaninogenica

Photodynamic therapy (PDT) has been commonly used in treating many diseases, such as cancer and infectious diseases. We investigated the different effects of PDT on three main pathogenic bacteria of periodontitis — Prevotella melaninogenica (P.m.), Porphyromonas gingivalis (P.g.) and Aggregatibacter actinomycetemcomitans (A.a.). The portable red light-emitting diode (LED) phototherapy device was used to assess the exogenous PDT effects with different light doses and photosensitizer concentrations (Toluidine blue O, TBO). The portable blue LED phototherapy device was used to assess the endogenous PDT effects with the use of endogenous photosensitizers (porphyrin) under different light doses. We found out that both exogenous and endogenous PDT were able to restrict the growth of all the three bacteria significantly. Moreover, the optimal PDT conditions for these bacteria were obtained through this in vitro screening and could guide the clinical PDT on periodontitis.

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Synergistic Effects of Silver Nanoparticles with Ethanolic Extract of Eucalyptus globules on Standard Pathogenic Bacteria in Vitro

Tabari Biomedical Student Research Journal ◽

10.18502/tbsrj.v2i3.4528 ◽

2020 ◽

Author(s):

Abolfazl Jafari Sales ◽

Afsoon Shariat

Keyword(s):

Silver Nanoparticles ◽

Pathogenic Bacteria ◽

Synergistic Effects ◽

Antibacterial Properties ◽

Ethanolic Extract ◽

Soxhlet Method ◽

Eucalyptus Plant ◽

Eucalyptus Globules

Introduction: Nowadays, with the increase of resistance due to overuse of synthetic chemical antibiotics, it seems necessary to find alternative drugs. The aim of this study was to compare the effects of silver nanoparticles and Eucalyptus globules (eucalyptus) ethanolic extract on standard bacteria of Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa and Escherichia coli. Material and Methods: In this experimental study, aerial parts of Eucalyptus plant were collected from Marand city and identified as Eucalyptus plant by botanists of Islamic Azad University, Ahar Branch. In this study, eucalyptus ethanolic extract was prepared by Soxhlet method and the antibacterial effects of eucalyptus extract at concentrations of 20, 30, 50 and 400 mg / ml and silver nanoparticles at concentrations of 10, 20, 40 and 80 μg / ml with agar well diffusion methods and tubular dilution were investigated. Results: The results showed that the ethanolic extract of Eucalyptus had more antibacterial properties compared to silver nanoparticles. Eucalyptus extract and silver nanoparticles had a greater effect on gram-positive bacteria. The effect of the combination of eucalyptus extract and silver nanoparticles was much greater than the effect of either. Conclusion: The results showed that silver nanoparticles in combination with eucalyptus extract have good antimicrobial activity against pathogenic bacteria. Therefore, this extract along with silver nanoparticles can be a good option for future studies in vivo to prepare antibacterial drugs.

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Antibacterial Properties of Local Malaysian Trigona Sp. Honey Towards Different of Pathogenic Bacteria in Vitro

Diyala Agricultural Sciences Journal ◽

10.52951/dasj.21130101 ◽

2021 ◽

Vol 13 (1) ◽

pp. 1-9

Author(s):

Fawzia Jassim Shalsh ◽

Ahmed Mohammed Khalaf ◽

Mohammed Hafiz ◽

Imad Ibrahim Ali

Keyword(s):

Minimum Inhibitory Concentration ◽

Pathogenic Bacteria ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Therapeutic Effects ◽

Disc Diffusion ◽

Diffusion Test

Honey is one of the oldest natural medicines known with a very high therapeutic value. Nowadays, in the medical field, several important therapeutic effects of honey have been elucidated. This study was conducted to reveal the antimicrobial activity of the commercially available local Malaysian Trigona sp. honey towards different pathogenic bacteria specifically Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, and Salmonella typhimurium. The Minimum inhibitory concentration and Minimum bactericidal concentration were determined by the disc-diffusion test and agar well diffusion test. Different concentration of the honey was tested in the disc-diffusion and agar well diffusion test. The results of these tests were in terms of Inhibition zone diameter. The results obtained from the current study are the dilution of different concentration of honey from Trigona sp. are very significant because the only net concentration of both of honey Trigon a sp. possessed antimicrobial properties in term of Minimum inhibitory concentration) and Minimum bactericidal concentration. The result also can say that Trigona sp. honey possessed antibacterial properties and can be used as alternative medicine in the veterinary field in the future.

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Isolation and Characterization of anti-Streptococcus Mutans Phage as a Possible Treatment Agent for Caries

10.20944/preprints202104.0045.v1 ◽

2021 ◽

Author(s):

Hadar Ben Zaken ◽

Reut Kraitman ◽

Shunit Coppenhagen-Glazer ◽

Leron Khalifa ◽

Sivan Alkalay-Oren ◽

...

Keyword(s):

Dental Caries ◽

Streptococcus Mutans ◽

Pathogenic Bacteria ◽

Antibacterial Effect ◽

Antibacterial Properties ◽

Human Saliva ◽

Whole Genome Sequence ◽

Isolation And Characterization

Streptococcus mutans is a key bacterium in dental caries- one of the most prevalent chronic infectious diseases. Conventional treatment both fails to specifically target the pathogenic bacteria and at-tempts to eradicate commensal bacteria as well. Thus, caries remains one of most common and challenging diseases. The use of bacterial viruses as anti-bacterial agents, is gaining interest worldwide. Hardly any phages were described against S. mutans. The objective of this study was to isolate anti-S. mutans phages and to characterize their antimi-crobial properties. Human saliva samples were filtered and screened for potential phages. Standard double-layered agar method was used for isolation. Whole genome sequence analysis and morphology visualization by TEM, were used for anti-S. mutans phage identification. Antibacterial properties were evaluated using clinical strains and ATCC strains of S. mutans in various states. Antibacterial effect was also tested on human cariogenic dentin. One phage against S. mutans was isolated and termed SMHBZ8. This phage showed effective lytic activity in vitro against both planktonic and biofilm S. mutans cultures. Moreover, the phage showed antibacterial effect when used on cariogenic dentin. The isolation and characterization of SMHBZ8 may be the first step in developing a potential phage therapy for dental caries.

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