scholarly journals Antioxidant and Antibacterial Capacities of Origanum vulgare L. Essential Oil from the Arid Andean Region of Chile and its Chemical Characterization by GC-MS

Metabolites ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 414 ◽  
Author(s):  
Mario J. Simirgiotis ◽  
Daniel Burton ◽  
Felipe Parra ◽  
Jéssica López ◽  
Patricio Muñoz ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Xanthomonas Campestris ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Gas Chromatography Mass Spectrometry ◽  
Origanum Vulgare ◽  
Andean Region ◽  
Oregano Oil

This study aimed to characterize the in vitro antioxidant and antibacterial properties of oregano (Origanum vulgare) essential oil, as well as its chemical composition. To our best knowledge, there are few studies on oregano grown in the arid Andes region, but none on the metabolites produced and their bioactivity. This work identified fifty metabolites by Gas Chromatography–Mass Spectrometry (GC-MS)—monoterpene hydrocarbons, oxygenated monoterpenes, phenolic monoterpenes, sesquiterpene hydrocarbons, and oxygenated sesquiterpenes—present in the essential oil of oregano collected in the Atacama Desert. The main components of essential oregano oil were thymol (15.9%), Z-sabinene hydrate (13.4%), γ-terpinene (10.6%), p-cymene (8.6%), linalyl acetate (7.2%), sabinene (6.5%), and carvacrol methyl ether (5.6%). The antibacterial tests showed that the pathogenic bacteria Staphylococcus aureus and Salmonella enterica and the phytopathogenic bacteria Erwinia rhapontici and Xanthomonas campestris were the most susceptible to oregano oil, with the lowest concentrations of oil necessary to inhibit their bacterial growth. Moreover, oregano oil showed antibacterial activity against bacteria associated with food poisoning. In conclusion, O. vulgare from the arid Andean region possesses an important antibacterial activity with a high potential in the food industry and agriculture.

scholarly journals Essential oil composition of Origanum vulgare and its application in substitution of synthetic chemical additives

2021 ◽  
pp. 94-99
Author(s):  
Sonia Heni ◽  
Hicham Boughendjioua ◽  
Salima Bennadja ◽  
Abdelghani Djahoudi
Keyword(s):  
Essential Oil ◽  
Pathogenic Bacteria ◽  
Gas Chromatography Mass Spectrometry ◽  
Chemical Components ◽  
Oil Composition ◽  
Chemical Additives ◽  
Origanum Vulgare ◽  
Oregano Essential Oil ◽  
Spoilage Bacteria

In this work, the gas  chromatography-mass spectrometry (GC/MS) analysis of the essential oil of Origanum vulgare. L. obtained by hydrodistylation was warried out and it allowed to identify 98.1% of its constituents. The main components are carvacrol (47.6%), thymol (16.6%), p-cymene (13.5%) andγ-terpinene (11.2%). The chemical components of the essence are distributed over five biochemical classes represented mainly by monoterpene phenols and monoterpene carbides. The extraction gave a yield of oil 2.8%. The minimum inhibitory concentration (MIC) of this oil was determined against food spoilage bacteria and pathogenic bacteria belonging to the genus Staphylococcus tested in vitro. This study aims to enhance the value of oregano essential oil by determining its chemical composition on the one hand, and by its application as a natural antibacterial preservative in substitution of synthetic chemical additives on the other hand. The sample was incorporated into a sensitive food matrix exposed to bacterial contamination: white meat. The bacteriostatic/bactericidal power of this bioactive extract was determined by a significant reduction in the number of bacteria and a clear increase in shelf life. Thus, it is possible to propose the use of this aromatic essence as a source of natural preservative.

Essential Oil and Ethanol Extract of Oregano (Origanum vulgare L.) from Armenian Flora as a Natural Source of Terpenes, Flavonoids and other Phytochemicals with Antiradical, Antioxidant, Metal Chelating, Tyrosinase Inhibitory and Antibacterial Activity

2019 ◽  
Vol 25 (16) ◽  
pp. 1809-1816 ◽  
Author(s):  
Armenuhi Moghrovyan ◽  
Naira Sahakyan ◽  
Anush Babayan ◽  
Naira Chichoyan ◽  
Margarit Petrosyan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Natural Source ◽  
Origanum Vulgare ◽  
Oregano Essential Oil ◽  
E Coli ◽  
Metal Chelating

Background: Origanum vulgare is used in Armenian cuisine as well as in folk medicine. The aim of this investigation was to determine the peculiarities of the chemical composition of Armenian flora’s oregano essential oil (EO) and ethanol extract (EE) and to characterize mainly antioxidant and antibacterial properties in vitro. Methods: EO of plant aerial parts was extracted by the hydro-distillation method, and the composition was analyzed by gas-chromatography (GC). EE was prepared using ethanol as a solvent. Antioxidant, antibacterial and other biological activity of EO and EE was determined using appropriate methods. Results: The terpenes were found to be the greatest part of oregano EO (β-caryophyllene epoxide - 13.3 %; β- caryophyllene -8.2 %; ο-cymene - 5.2 %). The flavonoid content of EE was 3.9±0.7 mg g-1 catechin equivalents. Antiradical activities of EO and EE expressed with half-maximum inhibitory concentrations were 1057 µg mL-1 and 19.97 µg mL-1. The antioxidant index in the case of using extract (1000 µg mL-1) was 77.3±1.5 %. The metal chelating activity of EE was 74.5±0.2 %. The tyrosinase inhibitory activities of EO, EE and arbutin were 26.5±0.3 %, 6.5±0.2 % and 50±0.1 %, respectively. Both EO and EE of oregano were able to suppress the growth of testmicroorganisms including clinical pathogen isolates, but have moderate antimicrobial activity. EO antibacterial activity against ampicillin-resistant Escherichia coli was similar to the activity against non-resistant strain. EE was not active against ampicillin-resistant E. coli in contrast to non-resistant one. Conclusion: The results indicate high antioxidant and moderate antibacterial as well as high antiradical, metal chelating, tyrosinase inhibitory activity of oregano EO and EE, which can be used as a natural source of terpenes, flavonoids and other phytochemicals in medicine, pharmaceutics, cosmetics and food industry. EO has antibacterial activity also against ampicillin-resistant E. coli.

scholarly journals Antibacterial Activity of Endostemon tereticaulis (Poir.) M. Ashby Essential Oil and Ethanolic Extract Against Resistant Pathogenic Bacteria

2020 ◽  
Vol 15 (9) ◽  
pp. 1934578X2095325
Author(s):  
Bala Namata Abba ◽  
Abderrahmane Romane ◽  
Amadou Tidjani Ilagouma
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Traditional Medicine ◽  
Pathogenic Bacteria ◽  
Ethanolic Extract ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Minimal Bactericidal Concentration

Endostemon tereticaulis (poir.) M.Ashby is a species of the Lamiaceae family present in Niger. This plant is used in traditional medicine due to its various biological potentialities. The present study investigated the chemical composition of the essential oil and the antibacterial activity of the essential oil and ethanolic extract of Endostemon tereticaulis against resistant pathogenic bacteria. Gas chromatography-mass spectrometry analysis of the essential oil led to the identification of 43 compounds representing 99.55% of the total essential oil. The major components were caryophyllene oxide (15.17%) followed by α-humulene (13.96%), α-copaene (11.75%), ( E)-β-caryophyllene (8.44%), and δ-cadinene (6.78%). The antibacterial activity was tested against multiresistant Acinetobacter baumannii P1483, Salmonella spp. H1548, extended-spectrum β-lactamase- Escherichia coli Bu8566, Enterobacter cloacae Bu147, Proteus mirabilis Bu190 , Pseudomonas aeruginosa (ATCC 27853), Klebsiella pneumoniae (ATCC 700603), Escherichia coli (ATCC 25922), Enterococcus faecium H3434, methicillin-resistant Staphylococcus aureus P1123, and Staphylococcus aureus (ATCC 25923). The antibacterial assays revealed that the essential oil was more active than the ethanolic extract against the studied bacteria with minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values ranging from 0.06 to 2 mg/mL. Also, the ethanolic extract was effective against the bacteria tested with MIC and MBC values ranging from 0.12 to 3 mg/mL. This study showed that Endostemon tereticaulis essential oil is rich in bioactive compounds. Ethanolic extract and essential oil exhibited potential antibacterial activity. These results provide a scientific basis for the use of this plant in traditional medicine. The current study described for the first time the antibacterial activity of Endostemon tereticaulis.

scholarly journals STUDY CYTOTOXICITY, ANTIBACTERIAL ACTIVITY, AND COMPOSITIONS BY FT-IR AND GC-MS FOR ORGANIC EXTRACT OF WHITE IRAQI DESERT TRUFFLE TIRMANIA NIVEA

2020 ◽  
Vol 17 (36) ◽  
pp. 18-31
Author(s):  
Ahmad khadem HACHIM ◽  
Rashid Rahim HATEET ◽  
Tawfik Muhammad MUHSIN
Keyword(s):  
Antibacterial Activity ◽  
Minimum Inhibitory Concentration ◽  
Cell Line ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Biological Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Bacterial Strains ◽  
Organic Extract

The purpose of the present work aimed at exploring the potential biochemical components and biological activities of an organic extract of the white truffle Tirmania nivea collected from the Iraqi desert, then test the organic extract against the Cytotoxicity on Human Larynx carcinoma cells and selected strains of pathogenic bacteria. Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry GC/MSS were used to analyze mycochemical compositions. The antibacterial activity and Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) was investigated using a disk diffusion agar method. The truffle extract's cytotoxicity effect against the larynx cell line (Hep-2) was assessed by the MTT assay (in vitro). FTIR results provided the presence of phenol, carboxylic acid, and alkane's functional group, The GC-MS analysis of T. nivea disclose the existence of nineteen compounds that can contribute to the pharmaceutical properties of the truffle. As for antibacterial activity result, A growth inhibition activity of truffle extract at (18-40 mm inhibition zones) against the tested pathogenic bacterial strains was detected, which minimum inhibitory concentration values ranged from 3.12 to 6.25 mg/mL for Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923) Respectively. The results of cytotoxicity shown that the organic truffle extract exhibited a high inhibitory rate (52.685%) against cell line (Hep-2) at a concentration of 1.56 ?g/mL. In this work, the results showed that the organic extracts of T. nivea are very promising as cancer cytotoxicity and antibacterial agent for future medical applications.

scholarly journals IN VITRO ANTIMICROBIAL ACTIVITY OF SOME ESSENTIAL OILS AGAINST BACTERIAL PATHOGENS CAUSING SKIN DISEASES IN VAPOR PHASE

2019 ◽  
pp. 113-115
Author(s):  
PURIT PATTANAPANIT ◽  
SUNISA MITHONGLANG ◽  
SUNITA MITHONGLANG ◽  
SURACHAI TECHAOEI
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Diffusion Method ◽  
Syzygium Aromaticum ◽  
Aromatic Plants ◽  
Volatile Oils

Objective: The objective of this study was to evaluate the antimicrobial activity of volatile oils from aromatic plants against pathogenic bacteria.Methods: Thai aromatic plants such as Pogostemon cablin (Blanco) Benth (Patchouli oil), Cymbopogon nardus Rendle (Citronella grass oil), Pelargoniumroseum (Geranium oil), Syzygium aromaticum (L.) Merrill and Perry (clove oil), Cinnamomum spp.(cinnamon oil), and Cymbopogon citratus (DC.) Stapf.(lemongrass oil) were selected. Essential oils were obtained by water distillation and were stored at 4°C until use. Five human pathogenic bacteria wereobtained from Thai traditional Medicine College, Rajamangala University of Technology, Staphylococcus epidermidis, Escherichia coli, Staphylococcusaureus, methicillin-resistant S. aureus (MRSA), and Pseudomonas aeruginosa. The antibacterial activity of volatile oils was determined by disc-diffusionassay. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each essential oil were determined.Results: Our study showed that 10% of essential oil from Cinnamomum spp. was the most potential against S. aureus, MRSA, and E. coli when assayedby disc-diffusion method with inhibition zones ranging from 37.66±0.57 to 45.33±1.15 mm and from 29.33±0.57 to 36.00±1.00 for lemongrass oilwith MIC and MBC of 1.25%.Conclusion: From this study, it can be concluded that some essential oils have potential antibacterial activity. The present investigation providessupport to the antibacterial properties of essential oils and will be applied to health-care product as aroma antibacterial products.

scholarly journals Antibacterial Activity and Mechanisms of Essential Oil from Citrus medica L. var. sarcodactylis

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1577 ◽  
Author(s):  
Ze-Hua Li ◽  
Ming Cai ◽  
Yuan-Shuai Liu ◽  
Pei-Long Sun ◽  
Shao-Lei Luo
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Micrococcus Luteus ◽  
Membrane Integrity ◽  
Bactericidal Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
Dodecanoic Acid ◽  
Food Borne ◽  
Citrus Medica

In this work, antibacterial activity of finger citron essential oil (FCEO, Citrus medica L. var. sarcodactylis) and its mechanism against food-borne bacteria were evaluated. A total of 28 components in the oil were identified by gas chromatography-mass spectrometry, in which limonene (45.36%), γ-terpinene (21.23%), and dodecanoic acid (7.52%) were three main components. For in vitro antibacterial tests, FCEO exhibited moderately antibacterial activity against common food-borne bacteria: Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Micrococcus luteus. It showed a better bactericidal effect on Gram-positive bacteria than Gram-negative. Mechanisms of the antibacterial action were investigated by observing changes of bacteria morphology according to scanning electron microscopy, time-kill analysis, and permeability of cell and membrane integrity. Morphology of tested bacteria was changed and damaged more seriously with increased concentration and exposure time of FCEO. FCEO showed a significant reduction effect on the growth rate of surviving bacteria and lead to lysis of the cell wall, intracellular ingredient leakage, and consequently, cell death.

scholarly journals In vitro Antibacterial Activity of Libanotis montana Essential Oil in Combination with Conventional Antibiotics

2014 ◽  
Vol 9 (2) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Dragoljub L. Miladinović ◽  
Budimir S. Ilić ◽  
Tatjana M. Mihajilov-Krstev ◽  
Jovana L. Jović ◽  
Marija S. Marković
Keyword(s):  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Hierarchical Cluster ◽  
Gas Chromatography Mass Spectrometry ◽  
Bacterial Strains ◽  
Microdilution Method ◽  
Broth Microdilution Method

The chemical composition and antibacterial activity were examined of Libanotis montana Crantz subsp. leiocarpa (Heuff.) Soó. (Apiaceae) essential oil. Gas chromatography and gas chromatography/mass spectrometry were used to analyze the chemical composition of the oil. The antibacterial activity was investigated by the broth microdilution method against thirteen bacterial strains. The interactions of the essential oil and three standard antibiotics: tetracycline, streptomycin and chloramphenicol toward five selected strains were evaluated using the microdilution checkerboard assay in combination with chemometric methods: principal components analysis and hierarchical cluster analysis. Sesquiterpene hydrocarbons were the most abundant compound class in the oil (67.2%), with β-elemene (40.4%) as the major compound. The essential oil exhibited slight antibacterial activity against the tested bacterial strains in vitro, but the combinations L. montana oil-chloramphenicol and L. montana oil-tetracycline exhibited mostly either synergistic or additive interactions. These combinations reduced the minimum effective dose of the antibiotics and, consequently, minimized their adverse side effects. In contrast, the association of L. montana essential oil and streptomycin was characterized by strong antagonistic interactions against Escherichia coli ATCC 25922. In the PCA and HCA analyses, streptomycin stood out and formed a separate group.

scholarly journals Antibacterial activity of Zataria multiflora essential oil and its main components against Pseudomonas aeruginosa

2017 ◽  
Vol 63 (3) ◽  
pp. 18-24 ◽  
Author(s):  
Mohaddese Mahboubi ◽  
Rezvan Heidarytabar ◽  
Elaheh Mahdizadeh
Keyword(s):  
Gas Chromatography ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Antibacterial Properties ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Zataria Multiflora ◽  
Main Components

Summary Introduction: In Iranian traditional medicine, Zataria multiflora Boiss (Lamiaceae family) is reputed due to its antiseptic effects. Objective: The purpose of this study was to evaluate the antibacterial and biofilm killing effects of Z. multiflora essential oil and main components against Pseudomonas aeruginosa. Methods: The main components of essential oil were identified by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The antibacterial properties of Z. multiflora oil and main components were determined by assessing the MIC and MBC values, and their inhibition percent of biofilm killing effects were determined by the evaluation of optical density. The role of each main component in these activities was determined according to the chemical profiles of essential oil. Results: Thymol (38.7%), carvacrol (30.6%), and p-cymene (8.3%) were main components of twenty five components of essential oil. Carvacrol had the higher role in antibacterial activity against P. aeruginosa, followed by thymol. P-cymene enhanced the antibacterial activities of thymol and carvacrol against P. aeruginosa. Carvacrol showed the weak role in biofilm killing effect. In spite of the low antibacterial activity of p-cymene against P. aeruginosa, it can enhance the antibacterial activity of thymol or carvacrol. Conclusion: Z. multiflora essential oil can be used for the management of P. aeruginosa infections. Determining the precise role of each components needs investigating in their behavior in different media.

scholarly journals Chemical Composition and Assessment of Antimicrobial Activity of Lavender Essential Oil and Some By-Products

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1829
Author(s):  
Alexandru Ciocarlan ◽  
Lucian Lupascu ◽  
Aculina Aricu ◽  
Ion Dragalin ◽  
Violeta Popescu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Xanthomonas Campestris ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Residual Water ◽  
By Products

The producers of essential oils from the Republic of Moldova care about the quality of their products and at the same time, try to capitalize on the waste from processing. The purpose of the present study was to analyze the chemical composition of lavender (Lavanda angustifolia L.) essential oil and some by-products derived from its production (residual water, residual herbs), as well as to assess their “in vitro” antimicrobial activity. The gas chromatography-mass spectrometry analysis of essential oils produced by seven industrial manufacturers led to the identification of 41 constituents that meant 96.80–99.79% of the total. The main constituents are monoterpenes (84.08–92.55%), followed by sesquiterpenes (3.30–13.45%), and some aliphatic compounds (1.42–3.90%). The high-performance liquid chromatography analysis allowed the quantification of known triterpenes, ursolic, and oleanolic acids, in freshly dried lavender plants and in the residual by-products after hydrodistillation of the essential oil. The lavender essential oil showed good antibacterial activity against Bacillus subtilis, Pseudomonas fluorescens, Xanthomonas campestris, Erwinia carotovora at 300 μg/mL concentration, and Erwinia amylovora, Candida utilis at 150 μg/mL concentration, respectively. Lavender plant material but also the residual water and ethanolic extracts from the solid waste residue showed high antimicrobial activity against Aspergillus niger, Alternaria alternata, Penicillium chrysogenum, Bacillus sp., and Pseudomonas aeroginosa strains, at 0.75–6.0 μg/mL, 0.08–0.125 μg/mL, and 0.05–4.0 μg/mL, respectively.

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