scholarly journals Optimization On Subcritical Fluid Extraction Of Zingiberene

2019 ◽  
Vol 1 (1) ◽  
pp. 18-21
Author(s):  
Vita Paramita ◽  
Mohamad Endy Yulianto ◽  
Indah Hartati ◽  
Udin Bahrudin ◽  
RTD Wisnu Broto
Keyword(s):  
Aging Time ◽  
Subcritical Water ◽  
Critical Value ◽  
Surface Modeling ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Green Solvent ◽  
Independent Variable ◽  
Subcritical Fluid Extraction ◽  
Subcritical Fluid

The establishment of subcritical fluid application for zingiberene extraction was studied by employing water as a green solvent. The zingiberene content was observed by using Liquid Chromatography-Mass Spectrofotometry and regarding to the independent variable of temperature, extraction time, ginger-solvent ratio and aging time. By applying subcritical water, the critical value of zingiberene content obtained was 0.036%.  This value was approached by the surface modeling of alpha for rotatability design which performed at 125  C, 20 min of extraction time, 0.08 of ginger to solvent ratio and 9 min of aging time of extraction parameters. The surface modeling was validated by plotting the observed and predicted value of zingiberene content and evidenced by the value of R-square at 0.76.

scholarly journals Subcritical Methanol Extraction of the Stone of Japanese Apricot Prunus mume Sieb. et Zucc.

Biomolecules ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1047
Author(s):  
Tomoaki Kawabata ◽  
Yoshie Tanaka ◽  
Asako Horinishi ◽  
Megumi Mori ◽  
Asao Hosoda ◽  
...  
Keyword(s):  
Methyl Ether ◽  
Subcritical Water ◽  
Prunus Mume ◽  
Japanese Apricot ◽  
Sinapyl Alcohol ◽  
Phenolic Constituents ◽  
Subcritical Fluid Extraction ◽  
Subcritical Fluid ◽  
Source Materials ◽  
By Products

The pits of Japanese apricot, Prunus mume Sieb. et Zucc., which are composed of stones, husks, kernels, and seeds, are unused by-products of the processing industry in Japan. The processing of Japanese apricot fruits generates huge amounts of waste pits, which are disposed of in landfills or, to a lesser extent, burned to form charcoal. Mume stones mainly consist of cellulose, hemicellulose, and lignin. Herein, we attempted to solubilize the wood-like carapace (stone) encasing the pit by subcritical fluid extraction with the aim of extracting useful chemicals. The characteristics of the main phenolic constituents were elucidated by liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR) analyses. The degrees of solubility for various treatments (190 °C; 3 h) were determined as follows: subcritical water (54.9%), subcritical 50% methanol (65.5%), subcritical 90% methanol (37.6%), subcritical methanol (23.6%), and subcritical isopropyl alcohol (14.4%). Syringaldehyde, sinapyl alcohol, coniferyl alcohol methyl ether, sinapyl alcohol methyl ether, 5-(hydroxymethyl)-2-furfural, and furfural were present in the subcritical 90% methanol extract. Coniferyl and sinapyl alcohols (monolignols) are source materials for the biosynthesis of lignin, and syringaldehyde occur in trace amounts in wood. Our current findings provide a solubilization method that allows the main phenolic constituents of the pits to be extracted under mild conditions. This technique for obtaining subcritical extracts shows great potential for further applications.

scholarly journals Optimisation of Protein Recovery from Arthrospira platensis by Ultrasound-Assisted Isoelectric Solubilisation/Precipitation

Processes ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 1586
Author(s):  
Ana Sánchez-Zurano ◽  
Ainoa Morillas-España ◽  
Cynthia Victoria González-López ◽  
Tomás Lafarga
Keyword(s):  
Food Industry ◽  
Functional Foods ◽  
Osmotic Shock ◽  
Arthrospira Platensis ◽  
Extraction Time ◽  
Protein Recovery ◽  
Solvent Ratio ◽  
Total Proteins ◽  
Cell Wall Disruption ◽  
Pre Treatment

A response surface methodology was used to optimise the solubilisation and precipitation of proteins from the cyanobacterium Arthrospira platensis. Two separate experiments were designed and conducted in a sequential manner. Protein solubilisation was affected by pH, extraction time, and biomass to solvent ratio (p < 0.001). Although spray-drying and the osmotic shock suffered when resuspending the dried biomass into distilled water led to a certain degree of cell wall disruption, the amount of protein that could be solubilised without an additional disruption step was in the range 30–60%. Sequential extractions improved protein solubilisation by less than 5%. For this reason, a pre-treatment based on sonication (400 W, 24 kHz, 2 min) had to be used, allowing the solubilisation of 96.2% of total proteins. Protein precipitation was affected by both pH and extraction time (p < 0.001). The optimised precipitation conditions, which were pH 3.89 over 45 min, led to a protein recovery of 75.2%. The protein content of the extract was close to 80%, which could be further increased by using different purification steps. The proteins extracted could be used in the food industry as technofunctional ingredients or as a source of bioactive hydrolysates and peptides for functional foods and nutraceuticals.

scholarly journals Ekstraksi Polifenol Total dari Daun Kadamba (Mitragyna speciosa Korth) Menggunakan Metode Choline Chloride-Sorbitol based Microwave Assisted Extraction

2019 ◽  
Vol 10 ◽  
pp. 131-134
Author(s):  
Ramila Hidayati ◽  
Dewi Rahmawati ◽  
Islamudin Ahmad
Keyword(s):  
Choline Chloride ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Green Solvent ◽  
Microwave Assisted ◽  
Extract Solution ◽  
Mitragyna Speciosa ◽  
Assisted Extraction ◽  
Total Extraction

This research was conducted to determine the influence of the use of the choline chloride-sorbitol based microwave assisted extraction (MAE) method against the total extraction of polyphenols from the leaf Kadamba (Mitragyna speciosa Korth). Simplisia extracted using solvent (green solvent) choline chloride-sorbitol with a ratio of 1:1; 1:2 and 1:3 g/g with sample and solvent ratio of 1:10 g/mL, microwave strength is 50% watt and performed for 10 minutes, the extract solution has been obtained and then done determination of total levels of polyphenols by using folin ciocalteu reagents and further measured its absorption with the UV-VIS spectrophotometer device at a wavelength of 770 nm. Based on the results of the study, obtained the levels of polyphenols from each comparison of solvent used against the sample of 334 mg of GAE/g samples (1:1 g/g solvent); 372 mg GAE/g samples (1:2 g/g solvents) and 299 mg of GAE/g samples (1:3 g/g solvents). Based on the results of the study can be concluded that the method of choline chloride-sorbitol based microwave assisted extraction (MAE) can be used to extract the polyphenols from Kadamba leaves easily, quickly and efficiently.

scholarly journals The Effect of NADES Ratio Solvent and Sonication Time in Extraction of Papaya Sap for Bio-insecticide Production

2018 ◽  
Vol 67 ◽  
pp. 03024
Author(s):  
M F Zikri ◽  
A Tibrizi ◽  
Y Marsino ◽  
T S Utami ◽  
R Arbianti ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Protein Concentration ◽  
Carica Papaya ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Sonication Time ◽  
Lowry Method ◽  
Ultrasonic Assisted ◽  
Tyrosine Concentration

Along with the increasing quantity of agriculture in Indonesia, should be followed by improving the quality of agriculture. Generally one of the indicators or parameters commonly used to indicate the quality of agriculture is the number of pests that damage plants in certain areas. The control of disturbing organisms on the plant can be minimized by the bio-insecticide production of Carica papaya which is tapped and extracted with ultrasonic-assisted using a mixed NADES solvent from Cholin chloride (ChCl) and Oxalic acid (Ox) with varying sonification time for 10, 20, 30 minutes and solvent ratio of 1: 2 and 2: 1. Bio-insecticide was produced by conducting qualitative enzyme activity of with lowry method and quantitatively using UV / Vis spectrophotometer aid with 750 nm wavelength and efficacy test. The best extraction result was obtained at NADES solvent with 1: 2 ratio with extraction time for 30 min with extraction time for 30 minutes yielding protein concentration equal to 7,23 ppm and tyrosine concentration equal to 7,9 ppm. Bio-insecticide samples effectively kill grayak caterpillars with 100% mortality within 5-7 days.

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