scholarly journals Optimisation of Protein Recovery from Arthrospira platensis by Ultrasound-Assisted Isoelectric Solubilisation/Precipitation

Processes ◽  
2020 ◽  
Vol 8 (12) ◽  
pp. 1586
Author(s):  
Ana Sánchez-Zurano ◽  
Ainoa Morillas-España ◽  
Cynthia Victoria González-López ◽  
Tomás Lafarga
Keyword(s):  
Food Industry ◽  
Functional Foods ◽  
Osmotic Shock ◽  
Arthrospira Platensis ◽  
Extraction Time ◽  
Protein Recovery ◽  
Solvent Ratio ◽  
Total Proteins ◽  
Cell Wall Disruption ◽  
Pre Treatment

A response surface methodology was used to optimise the solubilisation and precipitation of proteins from the cyanobacterium Arthrospira platensis. Two separate experiments were designed and conducted in a sequential manner. Protein solubilisation was affected by pH, extraction time, and biomass to solvent ratio (p < 0.001). Although spray-drying and the osmotic shock suffered when resuspending the dried biomass into distilled water led to a certain degree of cell wall disruption, the amount of protein that could be solubilised without an additional disruption step was in the range 30–60%. Sequential extractions improved protein solubilisation by less than 5%. For this reason, a pre-treatment based on sonication (400 W, 24 kHz, 2 min) had to be used, allowing the solubilisation of 96.2% of total proteins. Protein precipitation was affected by both pH and extraction time (p < 0.001). The optimised precipitation conditions, which were pH 3.89 over 45 min, led to a protein recovery of 75.2%. The protein content of the extract was close to 80%, which could be further increased by using different purification steps. The proteins extracted could be used in the food industry as technofunctional ingredients or as a source of bioactive hydrolysates and peptides for functional foods and nutraceuticals.

scholarly journals Phycocyanin content and nutritional profile of Arthrospira platensis from Mexico: efficient extraction process and stability evaluation of phycocyanin

BMC Chemistry ◽  
2021 ◽  
Vol 15 (1) ◽  
Author(s):  
Sanghamitra Khandual ◽  
Edgar Omar Lopez Sanchez ◽  
Hugo Espinosa Andrews ◽  
Jose Daniel Padilla de la Rosa
Keyword(s):  
Storage Temperature ◽  
Extraction Process ◽  
Arthrospira Platensis ◽  
Extraction Methods ◽  
Precipitation Method ◽  
Partial Purification ◽  
Natural Food ◽  
Solvent Ratio ◽  
C Storage ◽  
Dry Biomass

AbstractPhycocyanin is a blue natural food colorant with multiple health benefits. Here we propose an efficient phycocyanin extraction method from Arthrospira platensis from Mexico. Three extraction methods were applied to optimize the extraction process, using water and buffer as solvents, with three pH values at two agitation times. The highest phycocyanin, 54.65 mg/g, was extracted from dry biomass with water as a solvent using an ultrasonication bar. The optimum condition of extraction was determined to be 1:50 biomass/solvent ratio for dry biomass, with the freeze/thaw method for 20 min repeated twice, and then agitated at 120 rpm for 24 h. The phycocyanin content was 48.88 mg/g biomass, with a purity of 0.47. For scalable phycocyanin productivity, the sonication method is recommended as there is no statistical difference. The phycocyanin stability was best at − 20 °C storage temperature at pH 7 for 35 days. Partial purification with ammonium sulfate was found to be suitable as a fractional precipitation method, first at 0–20% and then 20–65%, to get purity nearly 1. Total protein was found to be 55.52%, and total amino acids after phycocyanin extraction was 33%. The maximum phycocyanin yield using water as a solvent was the most interesting result regardless of the method used for extraction.

scholarly journals Viability of Lactobacillus casei ATCC 393 and properties in Andean blackberry suspensions with probiotic and prebiotic characteristics

DYNA ◽  
2019 ◽  
Vol 86 (210) ◽  
pp. 179-186
Author(s):  
Zaira Tatiana Marin Arango ◽  
Misael Cortés Rodriguez ◽  
Olga Ines Montoya Campuzano ◽  
Julio Cesar Arango Tobón
Keyword(s):  
Zeta Potential ◽  
Mass Fraction ◽  
Lactobacillus Casei ◽  
Food Industry ◽  
Functional Foods ◽  
Total Solid ◽  
Dependent Variables ◽  
Solids Suspension ◽  
Probiotic Microorganism ◽  
Spray Dried

Food industry has grown in recent years mainly due to the supply of functional foods with probiotics and prebiotics. The viability of Lactobacillus casei and the properties of blackberry suspensions added with inulin were evaluated using a central design composed of the following factors: mass fraction of total solid blackberry concentrate (XTSBC) (0.103-0.120), [McFarland] (6-10), inulin (2.78-3.68%), and mass blackberry concentrate (BC)/mass inoculum (R) (5-20); and the dependent variables: moisture (M), pH, acidity, °Brix, total solids suspension (TSS), viscosity (μ), zeta potential (z), and Log CFU/g. Results of the experimental optimization of multiple responses were: XTSBC (0.103), [McFarland] (10), inulin (3.12%w/w), and R (18.5); being the dependent variables: M=86.7±0.0%, pH=2.74±0.01, acidity=3.1±0.0%, °Brix=11.0±0.1%, TSS=13.3±0.0%, µ=1288.0±20.1 cP, z=-13.3±0.3 mV and Log CFU/g=7.8±0.5. The probiotic microorganism Lb. casei ATCC 393 presents an adaptability in suspensions based on BC and inulin, which guarantee its viability and a possible use for obtaining an innovative spray-dried product.

scholarly journals Effect of ultrasound pre-treatment on formation of transglutaminase-catalysed soy protein hydrogel as a riboflavin vehicle for functional foods

2015 ◽  
Vol 19 ◽  
pp. 182-193 ◽  
Author(s):  
Hao Hu ◽  
Xinrong Zhu ◽  
Tan Hu ◽  
Imelda W.Y. Cheung ◽  
Siyi Pan ◽  
...  
Keyword(s):  
Soy Protein ◽  
Functional Foods ◽  
Pre Treatment

Analyzing and optimizing the carbon utilization and lipid yield at different light intensities for Scenedesmus arcuatus

2021 ◽  
Vol 25 (11) ◽  
pp. 1-10
Author(s):  
K. Vasumathi ◽  
Raja Vadivu G. Nadana ◽  
E.M. Nithiya ◽  
K. Sundar ◽  
M. Premalatha
Keyword(s):  
Light Intensity ◽  
Large Scale ◽  
Biomass Yield ◽  
Osmotic Shock ◽  
High Light Intensity ◽  
Carbon Utilization ◽  
Lipid Yield ◽  
The Face ◽  
Photosynthetic Microorganism ◽  
Pre Treatment

Microalgae, the photosynthetic microorganism growing abundantly in marine and aquatic ecosystems, are potential source for biological sequestration of CO2. The carbon uptake differs in the presence of other nutrients, light intensity etc. The biomass yield of Scenedesmus arcuatus var capitatus was studied based on the Face Centred Central Composite design (FCCD) of Response Surface Methodology (RSM) for nitrate, phosphate and carbonate under different conditions (laboratory, room and sunlight conditions). Various pre-treatments (osmotic shock, autoclaving, microwave and ultrasonication) were employed to find the best method for maximum lipid yield. The biomass yield reached a maximum of 1 g/L under sunlight conditions of nitrate concentration 500 ppm and carbonate 2000 ppm. The laboratory conditions resulted in a biomass yield of 0.59 g/L at 500 ppm nitrate, 1000 ppm carbonate and 250 ppm phosphate. Under room conditions, the yield was very low (0.11 g/L). Osmotic shock resulted in higher lipid yield than the other pre-treatment methods. The ability of Scenedesmus arcuatus to uptake high carbon under sunlight conditions and to adapt to high light intensity and fluctuations in light intensity concludes that this species is suitable for large-scale open pond cultivation for CO2 sequestration and production of metabolites.

Alkaline pre-treatment of waste chestnut shell from a food industry to enhance cadmium, copper, lead and zinc ions removal

2012 ◽  
Vol 184 ◽  
pp. 147-155 ◽  
Author(s):  
Gonzalo Vázquez ◽  
Olga Mosquera ◽  
M. Sonia Freire ◽  
Gervasio Antorrena ◽  
Julia González-Álvarez
Keyword(s):  
Food Industry ◽  
Zinc Ions ◽  
Lead And Zinc ◽  
Chestnut Shell ◽  
Pre Treatment ◽  
Copper Lead

Protein Extraction from Spirulina platensis by Using Ultrasound Assisted Extraction: Effect of Solvent Types and Extraction Time

2021 ◽  
Vol 872 ◽  
pp. 33-37
Author(s):  
Kong Sela ◽  
Wiratni Budhijanto ◽  
Arief Budiman
Keyword(s):  
Spirulina Platensis ◽  
Protein Extraction ◽  
Solvent Mixture ◽  
Industrial Applications ◽  
Extraction Time ◽  
Protein Recovery ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Solvent Volume

Protein is a substantial nutrition that essentially required by human. Spirulina platensis (Spp), well known as protein source could be a significant source to be used for many industrial applications. This study was investigated the effectiveness of ultrasound assisted extraction (UAE) method for protein extraction from Spp at various composition of solvent mixture and extraction time. Ethanol and mixture of methanol-ethanol were used as solvent. Extraction was conducted by varying ratios of solvent to biomass at 10:1, 12.5:1, and 15:1 (v/w), and extraction time (20, 35, and 50 min). Optimum protein recovery from dry Spp was 42.55 ± 0.43% obtained by using 20 ml of the mixture of methanol and ethanol at 50 min of extraction time. This study also conducted that mixture of methanol and ethanol was a better solvent on improving the ultrasound assisted extraction, as indicated by high protein recovery with less amount of solvent volume used.

scholarly journals The Effect of NADES Ratio Solvent and Sonication Time in Extraction of Papaya Sap for Bio-insecticide Production

2018 ◽  
Vol 67 ◽  
pp. 03024
Author(s):  
M F Zikri ◽  
A Tibrizi ◽  
Y Marsino ◽  
T S Utami ◽  
R Arbianti ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Protein Concentration ◽  
Carica Papaya ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Sonication Time ◽  
Lowry Method ◽  
Ultrasonic Assisted ◽  
Tyrosine Concentration

Along with the increasing quantity of agriculture in Indonesia, should be followed by improving the quality of agriculture. Generally one of the indicators or parameters commonly used to indicate the quality of agriculture is the number of pests that damage plants in certain areas. The control of disturbing organisms on the plant can be minimized by the bio-insecticide production of Carica papaya which is tapped and extracted with ultrasonic-assisted using a mixed NADES solvent from Cholin chloride (ChCl) and Oxalic acid (Ox) with varying sonification time for 10, 20, 30 minutes and solvent ratio of 1: 2 and 2: 1. Bio-insecticide was produced by conducting qualitative enzyme activity of with lowry method and quantitatively using UV / Vis spectrophotometer aid with 750 nm wavelength and efficacy test. The best extraction result was obtained at NADES solvent with 1: 2 ratio with extraction time for 30 min with extraction time for 30 minutes yielding protein concentration equal to 7,23 ppm and tyrosine concentration equal to 7,9 ppm. Bio-insecticide samples effectively kill grayak caterpillars with 100% mortality within 5-7 days.

scholarly journals Natural Choline from Egg Yolk Phospholipids Is More Efficiently Absorbed Compared with Choline Bitartrate; Outcomes of A Randomized Trial in Healthy Adults

Nutrients ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 2758 ◽  
Author(s):  
Lotte Smolders ◽  
Nicole J.W. de Wit ◽  
Michiel G.J. Balvers ◽  
Rima Obeid ◽  
Marc M.M. Vissers ◽  
...  
Keyword(s):  
Randomized Trial ◽  
Food Industry ◽  
Functional Foods ◽  
Area Under The Curve ◽  
Egg Yolk ◽  
Choline Uptake ◽  
Double Blind ◽  
Natural Form ◽  
Choline Bitartrate ◽  
Essential Nutrient

Choline is a vitamin-like essential nutrient, important throughout one’s lifespan. Therefore, choline salts are added to infant formula, supplements and functional foods. However, if choline is present in a natural form, e.g. bound to phospholipids, it may be more efficiently absorbed. The study’s aim was to evaluate if choline uptake is improved after consumption of an egg yolk phospholipid drink, containing 3 g of phospholipid bound choline, compared to a control drink with 3 g of choline bitartrate. We performed a randomized, double blind, cross-over trial with 18 participants. Plasma choline, betaine and dimethylglycine concentrations were determined before and up to six hours after consumption of the drinks. The plasma choline response, as determined by the incremental area under the curve, was four times higher after consumption of the egg yolk phospholipid drink compared with the control drink (p < 0.01). Similar outcomes were also observed for choline’s main metabolites, betaine (p < 0.01) and dimethylglycine (p = 0.01). Consumption of natural choline from egg yolk phospholipids improved choline absorption compared to consumption of chemically produced choline bitartrate. This information is of relevance for the food industry, instead of adding choline-salts, adding choline from egg yolk phospholipids can improve choline uptake and positively impact health.

Determination of Parabens from Water Samples Using Cloud Point Extraction, Vortex Extraction and Liquid–Liquid Extraction Method Coupled with High Performance Liquid Chromatography

2020 ◽  
Vol 17 (2) ◽  
pp. 765-772
Author(s):  
Noorashikin Md Saleh ◽  
N. M. Hafiz ◽  
Nik Nur Atiqah NikWee
Keyword(s):  
Cloud Point ◽  
Water Samples ◽  
Extraction Method ◽  
Optimum Parameter ◽  
Cloud Point Extraction ◽  
Liquid Extraction ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Liquid Liquid Extraction ◽  
Method Of Extraction

A straightforward and efficient way for extraction of parabens that is methylparaben, ethylparaben, propylparaben and benzylparaben in environmental water samples was developed through optimizing parameters for each method of extraction. In this study, methods involved were cloud point extraction, vortex extraction, and liquid–liquid extraction. The parameters affecting the method of extraction were such as salt concentration, surfactant concentration, type of solvent, temperature, ratio of solvent to water and extraction time. The optimum parameter for cloud point extraction method were 1.0 M of salt, 1.0% v/v of surfactant, ratio of surfactant to water is 1:1, extraction time is 1 minute at 30 °C while vortex extraction method, optimum parameter is 1.0 M salt, using acetonitrile as a solvent, ratio 1 solvent: 4 water, and extracted at 1 minute. For the liquid–liquid extraction method, the optimum parameter was at 1.0 M salt, acetonitrile as a solvent, ratio of solvent to water is 1:1 and extraction time at 1 minute. The correlation coefficient for the calibration of paraben at concentration 0.2 ppm–1.0 ppm was in the range from 0.9703 to 0.9942. The limit of detection of studied paraben were 0.1627, 0.0837, 0.1156 and 0.1918 ppm, respectively. Percentage recovery for cloud point extraction, vortex extraction and liquid–liquid extraction were between 41%–147.9%, 26.5%–134.7%, and 31.4%–142.4% respectively. Each sample is repeated with triplication which the value of the relative standard deviation is less than 17.9%. Thus, the most suitable, efficient and effective method in extraction of paraben from water samples is cloud point extraction. The cloud point extraction shows the potential to be explore on the future extraction of others organic pollutants from water samples.

scholarly journals Extraction of Illegal Dyes from Red Chili Peppers with Cholinium-Based Deep Eutectic Solvents

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Shuqiang Zhu ◽  
Dongling Liu ◽  
Xinyue Zhu ◽  
Along Su ◽  
Haixia Zhang
Keyword(s):  
Choline Chloride ◽  
Deep Eutectic Solvents ◽  
Raw Material ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Green Solvents ◽  
Hydrogen Bond Donor ◽  
Relative Standard ◽  
Orange G

Deep eutectic solvents (DESs) as a new kind of green solvents have been used to extract bioactive compounds but there are few applications in extracting chrysoidine dyes. In this study, we developed an ultrasonic-assisted extraction method with choline chloride/hydrogen bond donor (ChCl/HBD) DES for the extraction of chrysoidine G (COG), astrazon orange G (AOG), and astrazon orange R (AOR) in food samples. Some experimental parameters, such as extraction time, raw material/solvent ratio, and temperature, were evaluated and optimized as follows: the ratio of ChCl/HBD, 1 : 2 (v/v); the ratio of sample/DES, 1 : 10 (g/mL); extraction time, 20 min; extraction temperature, 50°C. Under the optimized conditions, the limits of detection (μg/mL) were 0.10 for COG and 0.06 for AOG and AOR. The relative standard deviations were in the range of 1.2–2.1%. The recoveries of the three dyes were in the range of 80.2–105.0%. By comparing with other commonly used solvents for extracting chrysoidine dyes, the advantages of DESs proved them to be potential extraction solvents for chrysoidine G, astrazon orange G, and astrazon orange R in foods.

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