scholarly journals In vitro and in vivo Antibacterial Activity of Ethanolic Extract of Sweet Basil (Ocimum basilicum L.) Leaves against Escherichia coli in Experimentally Infected Rats

2015 ◽  
Vol 3 (6) ◽  
pp. 308-320 ◽  
Author(s):  
Orooba Mohammed Saeed Ibrahim
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Ocimum Basilicum ◽  
Sweet Basil

scholarly journals In vitro and in vivo evaluation of antibacterial activity of Bridelia ferruginea extracts on some clinical isolates

2018 ◽  
Vol 7 (4) ◽  
pp. 392-398
Author(s):  
B.T Yunana ◽  
◽  
B. B Bukar ◽  
J. C Aguiyi ◽  
◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bark Extract ◽  
Root Extract ◽  
Root Bark ◽  
Zone Of Inhibition ◽  
Bridelia Ferruginea

The ethanol extracts of root, bark and leaf of Bridelia ferruginea was investigated for antibacterial activity against clinical isolate of Staphylococcus aureus and Escherichia coli. The extracts had significant antibacterial activity in vitro at concentration of 25 mg/ml, 50 mg/ml, 100 mg/ml and 200 mg/ml and in vivo at dose of 50 mg/kg and 100 mg/kg. The root extract in vitro had the highest zone of inhibition, followed by the bark extract for both Staphylococcus aureus and Escherichia coli. The concentration of 200 mg/ml had the highest zone of inhibition in vitro. The minimum inhibitory concentration (MIC) showed a decreasing inhibitory effect of the plant extracts for both Staphylococcus aureus and Escherichia coli as the concentration decreases with root having 3.125 mg/ml, bark having 6.25 mg/ml and leaf having 25 mg/ml for Staphylococcus aureus and Escherichia coli. Likewise, the minimum bactericidal concentration (MBC) showed decreasing bactericide effects with decrease concentration with root having 12.5 mg/ml, bark having 12.5 mg/ml and leaf having 25 mg/ml for Escherichia coli while root had 6.25mg/ml, bark had 12.5mg/ml and leaf had 25mg/ml for Staphylococcus aureus. The in vivo investigation showed that the root and bark extract exhibited antibacterial activity on both Staphylococcus aureus and Escherichia coli at doses of 100mg/kg and 50mg/kg; the root extract had higher activity than the bark and root/bark combined. The dose of 100 mg/kg had the highest colonies reduction for Staphylococcus aureus and Escherichia coli in vivo. Preliminary phytochemical screening of root, bark and leaves of Bridelia ferruginea revealed the presence of tannins, flavonoids, carbohydrates, cardiac glycoside (root, bark and leaves), saponins (root and bark). The presence of tannins, saponins, flavonoid, cardiac glycoside and carbohydrate in the bark and root extracts of the plant indicates that the bark and root extracts were pharmacological importance

scholarly journals In vitro and in vivo, antibacterial effect of lactobacillus and ethanolic extract of sage Salvia officinalis on enteropathogenic E.coli (EPEC)

2012 ◽  
Vol 6 (2) ◽  
pp. 50-56
Author(s):  
Amina N. AlThawani ◽  
Rasha abdul-Hussein Mahood ◽  
Hazim I. Abdul-Barrey
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Effect ◽  
Ethanolic Extract ◽  
Salvia Officinalis ◽  
Diffusion Method ◽  
High Concentration ◽  
Liver And Kidney ◽  
Cell Free Filtrate

he antibacterial effect of Lactobacillus cell-free filtrate and ethanolic extract of sage Salvia officinalis on enteropathogenic E.coli (EPEC) were investigated in vitro and in vivo. In vitro, antibacterial activity of Lactobacillus and ethanolic extract of sage were determined by using well diffusion method. The results of ethanolic extract of sage showed moderate antibacterial activity even with high concentration of extract 20mg/ml with maximum inhibition zone 18mm. while, of Lactobacillus cell-free filtrate presented high antibacterial activity against E.coli (24mm) in vivo, thirty two albino male mice(age 8-10 weeks, weight ranged 23-27 gram) were used in this experiment. The animals were divided into four equal groups, include positive and negative controls. Histological analysis of intestine, liver and kidney showed that the mice infected with EPEC induce attaching and effacing (A/E) lesions and loss of microvillus actins rootlets as well as microvillus fragmentation and no significant changes in liver and kidney tissues of mice infected with EPEC. Normal microvilli and mucosal morphology observed in mice infected and treated with Lactobacillus and ethanolic extract of sage.

scholarly journals IN VITRO ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF SEEDPOD AND QUERCETIN OF NELUMBO NUCIFERA GAERTN

2019 ◽  
Vol 12 (1) ◽  
pp. 164
Author(s):  
Ruvanthika Pn ◽  
Manikandan S
Keyword(s):  
Antibacterial Activity ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Nelumbo Nucifera ◽  
Diffusion Method ◽  
Dependent Manner ◽  
E Coli ◽  
In Vitro Antibacterial Activity

Objective: The objective of the study was to evaluate whether ethanolic extracts of Nelumbo nucifera (EENN) seedpod and quercetin (active component of NN) possess antibacterial proprieties against Gram (-) bacteria such as Escherichia coli and Pseudomonas aeruginosa and Gram (+) bacteria such as Staphylococcus aureus. Methods: Antibacterial activities of EENN seedpod and quercetin were investigated using disc diffusion method, minimum inhibitory concentration against E. coli and P. aeruginosa and Gram (+) bacteria such as S. aureus. Results: The antibacterial activity of both EENN seedpod and quercetin was found to be increased in dose-dependent manner. The maximum zone of inhibition was exhibited by both EENN seedpod and quercetin against E. coli (14 mm and 15 mm) and P. aeruginosa (13 mm and 15 mm). Gram-negative bacteria were more susceptible to the EENN seedpod extract and quercetin than Gram-positive bacteria.Conclusion: The results of the present study suggested that the effect of EENN seedpod and quercetin against the tested bacteria in vitro may contribute to the in vivo activities of the EENN seedpod and quercetin.

scholarly journals Growth and pathogenesis of Enteropathogenic E.coli as affected by bacteriocin produced and purified from Lactobacillus isolates with or without Quercus infectoria

2012 ◽  
Vol 36 (0E) ◽  
pp. 1-7
Author(s):  
Hassan A. Abdul-Ratha
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Synergistic Effect ◽  
Herbal Drugs ◽  
Bacteriocin Activity ◽  
Recovery Yield ◽  
Quercus Infectoria

The antimicrobial activity of Lactobacilli has been widely exploited for prevention offood –borne pathogens e.g.: Escherichia coli being the major cause of diarrhea especially inchildren, because of bacteriocin activity and the importance of herbal drugs, hence this studywas designed to evaluate the synergistic effect of plant extract and bacteriocin produced byLactobacillus on the growth and pathogenesis of Enteropathogenic Ecoli.1. The Plantaricin production was induced by adding the mutagenic agent Mitomycin C.2. Purification of Plantaricin was made by heating crude plantaricin at 80ºC for 10min andthen purified by two steps method including extraction with n-butanol followed by gelfiltration chromatography on Sepharose 6B column. The results showed that the specificactivity was 1600 AU/mg protein with 8 purification folds and 12% recovery yield.3. The antibacterial activity of Quercus infectoria with concentration 300 mg/ml wasshowed highly antibacterial activity in vitro and in vivo.4. The result showed synergistic effect of Plantaricin with Quercus infectoria extract afterexperimental infection that induced by orally dosing with Escherichia coli in vivo. A result ofhistopathological study was recorded recovery of tissue.

scholarly journals In-Vivo In-Vitro Screening of Ocimum basilicum L. Ecotypes with Differential UV-B Radiation Sensitivity

Horticulturae ◽  
2021 ◽  
Vol 7 (5) ◽  
pp. 101
Author(s):  
Haana Mosadegh ◽  
Alice Trivellini ◽  
Rita Maggini ◽  
Antonio Ferrante ◽  
Luca Incrocci ◽  
...  
Keyword(s):  
Radiation Sensitivity ◽  
Ocimum Basilicum ◽  
Plant Production ◽  
Sweet Basil ◽  
Closed Type ◽  
Geographic Origins ◽  
Protection Mechanisms ◽  
Uv Absorbing Compounds

Elevated UV-B radiation (UV-B) has been previously reported to affect plant development, physiology, and promote the biosynthesis of UV-absorbing compounds. Sweet basil (Ocimum basilicum L.) is an aromatic herb, widely cultivated worldwide for its use in the food, pharmaceuticals, and cosmetics industry. This species exhibits high diversity among different ecotypes based on their geographical locations. There has been little research on intra-specific photosynthetic and metabolic differences in UV-B tolerance across ecotypes from different geographical areas. This study evaluated the protection responses to high UV-B radiation of nine O. basilicum accessions with different geographic origins. Specifically, the changes in chlorophyll a fluorescence parameters and the leaf rosmarinic acid (RA) compound were assessed using an “in vivo-vitro system” in a closed-type plant production system. Our results revealed a significant variation in UV-B protection mechanisms among accessions when plants were treated with high UV-B doses. The accumulation of RA increased significantly by UV-B light treatment in OCI142, OCI148, OCI30, OCI160, and OCI102, with the highest concentration measured in OCI160 plants. This ecotype showed the highest value of the Fv/Fm ratio, 0.70, after 48 h. Recovery of leaf functionality was more rapid in OCI160 than in other sweet basil accessions, which may indicate better photosynthetic capacity associated with enhanced biosynthesis of UV absorbing compounds. This study shows that the biosynthesis of the UV-absorbing compound (RA) represents an effective mechanism to reduce the photoinhibitory and photooxidative damage caused by high UV stress.

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK METANOL BATANG BIDARA LAUT (Strychnos ligustrina) TERHADAP BAKTERI PATOGEN

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Edy Kurniawan ◽  
Dwi Soelistya Dyah Jekti ◽  
Lalu Zulkifli
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Pathogenic Bacteria ◽  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Klebsiella Pneumonia ◽  
E Coli

Abstract : Strychnos ligustrina stem has been empirically used by the people of West Nusa Tenggara and Bali in the treatment of malaria, tooth ache and diarrhea, but there is no scientific data that supports it. This study aims to determine and prove the antibacterial activity of Strychnos ligustrina methanol extract to pathogenic bacteria in vitro and in vivo. This research is an experimental study conducted by measuring the inhibition zone (mm) growth of pathogenic bacteria, determining minimum inhibitory concentration (MIC) and minimum killing concentration (MKC) in vitro, and determining the percentage of antibacterial activity of methanol extract of S. ligustrina stem in vivo. The experiment was conducted using 4 groups of concentrations of S. ligustrina stem methanol extract in an in vitro study of 25, 50, 75, and 100% with ciprofloxacin as a positive control and aquadest as a negative control. In vivo studies experiments were carried out using 6 treatment groups of test animals male mice Balb / c (Mus musculus). The in vitro test results showed that methanol extract of S. ligustrina stems was able to inhibit the growth of pathogenic bacteria with medium categories of clinical isolates of Staphylococcus aureus and categories of weaks to Klebsiella pneumonia and Escherichia coli isolates. The minimum inhibitory concentration (MIC) for S. aureus and K. pneumonia bacteria isolates was at a concentration of 25% while for E. coli isolates at a concentration of 30%. The methanol extract of the S. ligustrina stem has no killing power against the pathogenic bacteria tested. Antibacterial activity in vivo was able to inhibit the growth of S. aureus pathogenic bacteria by 6.60% (at 25% concentration), 8.62% (at 50% concentration), and 17.31% (at 100% concentration), against K. pneumonia was 11.85% (at 25% concentration), 51.21% (at 50% concentration), and 65.92% (at 100% concentration), against E. coliat 19.18% (at concentration 25%), 29.98% (at 50% concentration), and 40.88% (at 100% concentration). Methanol extract of S. ligustrina stem proved to have antibacterial activity in vitro and in vivo. Key words: Srychnos ligustrina, pathogenic bacteria, antibacterial, in vitro, in Vivo. Abstrak : Strychnos ligustrina secara empiris  telah digunakan oleh masyarakat Nusa Tenggara Barat dan Bali dalam pengobatan penyakit malaria, sakit gigi, dan diare, tetapi belum ada data ilmiah yang mendukung. Penelitian ini bertujuan untuk menentukan dan membuktikan aktivitas antibakteri ekstrak metanol batang bidara laut terhadap bakteri patogen secara in vitrodan in vivo. Penelitian ini merupakan penelitian eksperimental yang dilakukan dengan mengukur zona hambat (mm) pertumbuhan bakteri patogen, menentukan konsentrasi hambat minimum (KHM) dan konsentrasi bunuh minimum (KBM) secara in vitro, serta menentukan persentase aktivitas antibakteri ekstrak metanol batang bidara laut secara in vivo. Percobaan dilakukan menggunakan 4 kelompok konsentrasi ekstrak metanol batang bidara laut pada penelitian in vitro yaitu 25, 50, 75, dan 100% dengan ciprofloxacin sebagai kontrol positif serta aquadest sebagai kontrol negatif. Pada penelitian in vivo percobaan dilakukan menggunakan 6 kelompok perlakuan hewan uji mencit jantan galur Balb/c (Mus musculus). Hasil uji in vitro menunjukkan ekstrak metanol batang bidara laut mampu menghambat pertumbuhan bakteri patogen dengan kategori sedang terhadap Staphylococcus aureus isolat klinis dan kategori lemah terhadap Klebsiella pneumonia dan Escherichia coli isolat klinis. Nilai konsentrasi hambat minimum (KHM) untuk isolat bakteri S. aureus dan K. pneumoniae adalah pada konsentrasi 25% sedangkan untuk isolat E. coli pada konsentrasi 30%. Ekstrak metanol batang bidara laut tidak memiliki daya bunuh terhadap bakteri patogen yang diuji. Aktivitas antibakteri secara in vivo mampu menghambat pertumbuhan bakteri patogen S. aureus sebesar 6,60% (pada konsentrasi 25%), 8,62% (pada konsentrasi 50%), dan 17,31% (pada konsentrasi 100%), terhadap K. pneumonia sebesar 11,85% (pada konsentrasi 25%), 51,21% (pada konsentrasi 50%), dan 65,92% (pada konsentrasi 100%),   terhadap E. coli sebesar 19,18% (pada konsentrasi 25%), 29,98% (pada konsentrasi 50%), dan 40,88% (pada konsentrasi 100%). Ekstrak metanol batang bidara laut terbukti memiliki aktivitas antibakteri secara in vitro dan in vivo. Kata kunci: Srychnos ligustrina, bakteri patogen, antibakteri, in vitro, in vivo

scholarly journals ANTI-PSEUDOMONAS AERUGINOSA DRUG

2018 ◽  
Vol 25 (10) ◽  
pp. 1574-1580
Author(s):  
Yasir Mehmood ◽  
Muhammad Imran Ashraf ◽  
Shazana Rana ◽  
Humayun Riaz ◽  
Syed Atif Raza ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Synergistic Effect ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Bark Extract ◽  
In Vitro Susceptibility ◽  
Inner Bark

Objectives: In this research we assess in-vitro susceptibility of Pseudomonasaeruginosa (P. aeruginosa) using the ethanolic extract of medicinal plant Tabebuia impetiginosa(dried inner bark). To evaluate the synergistic effect of ethanolic extract of Tabebuia impetiginosacombine with ciprofloxacin In-vitro anti-Pseudomonas aeruginosa activities of the extracts andciprofloxacin were confirmed, and synergism was verified for this combine extracts. StudyDesign: Experimental study. Period: October 2016 to February 2017. Place of Experiment:Rashid Latif Medical College, Lahore. Method: Diffusion method tests are mostly qualitativemethods that are used to identify the antimicrobial activity, resistance and synergistic effect. Thefresh plants inner bark was grinded and soaked in 95% ethanol for extraction. The antibacterialsensitivity of this compound against P. aeruginosa was assessed using the diffusion method.About 1000mg of grinded menstruum was added in 800ml of petroleum ether in a conicalflask and adjust in rotary shaker at 100 rpm for 12 hours and then the final extract was filteredwith 0.45mμ filter membrane and centrifuged at 2000rpm for 15 minutes. The final extract wasredissolved in ciprofloxacin solution (10 for bioassay analysis. Results: We concludedthat the fresh ethanolic extract of Tabebuia impetiginosa with ciprofloxacin has high antibacterialpotency against P. aeruginosa which is prominent then a single. However this was not pureextract and if it is refined then it might gives significant antibacterial activity at low concentration.There is still need to test Tabebuia impetiginosa extract for antibacterial activity and to checksynergistic effect with other drugs in-vivo against Pseudomonas aeruginosa. Extract presentedthe highest synergism rate with antimicrobial drug. Conclusion: In-vitro study showed Tabebuiaimpetiginosa fresh inner bark extract with ciprofloxacin dilution have significant antibacterialactivity against Pseudomonas aeruginosa with P.value <0.001. Isolates were susceptible tothis combine solution with mean zone diameter of 16.15 ± 0.95 mm and no regrowth wasnoticed. In the present research the synergistic effect of ciprofloxacin antibiotic with Tabebuiaimpetiginosa ethanolic extract were observe against Pseudomonas aeruginosa bacteria.

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