Volatile compounds produced from the cultured cells of thyme (Thymus vulgaris,L.)

Honey bees are essential to sustaining ecosystems, contributing to the stability of biodiversity through pollination. Today, it is known that the failure of pollination leads irremediably to the loss of plant cultures and, as a consequence, inducing food security issues. Bees can be affected by various factors, one of these being Nosema spp. which are protozoans specifically affecting adult honey bees and a threat to bee populations around the world. The composition of the phytotherapeutic product (Protofil®) for treating nosemosis was analyzed from a biochemical point of view. The most concentrated soluble parts in the phytotherapeutic association were the flavonoids, most frequently rutin, but quercetin was also detected. Additionally, the main volatile compounds identified were eucalyptol (1.8-cineol) and chavicol-methyl-ether. To evaluate the samples’ similarity–dissimilarity, the PCA multivariate statistical analysis, of the gas-chromatographic data (centered relative percentages of the volatile compounds), was applied. Statistical analysis revealed a significant similarity of Protofil® with the Achillea millefolium (Yarrow) samples and more limited with Thymus vulgaris (Thyme) and Ocimum basilicum (Basil), and, respectively, a meaningful dissimilarity with Taraxacum officinale (Dandelion). The results have shown a high and beneficial active compounds concentration in the analyzed herbs. High similarity with investigated product recommending the Protofil®, as the treatment compatible with producing organic honey.

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Essential Oils of Seven Lamiaceae Plants and Their Antioxidant Capacity

Molecules ◽

10.3390/molecules26133793 ◽

2021 ◽

Vol 26 (13) ◽

pp. 3793

Author(s):

David Aebisher ◽

Jan Cichonski ◽

Ewa Szpyrka ◽

Sygit Masjonis ◽

Grzegorz Chrzanowski

Keyword(s):

Oxidative Stress ◽

Essential Oils ◽

Antioxidant Capacity ◽

Volatile Compounds ◽

Tumor Development ◽

Thymus Vulgaris ◽

Natural Medicine ◽

Phenolic Components ◽

Phenolic Constituents ◽

High Level

Oxidative stress has been reported as a cause of many diseases like Parkinson′s, Alzheimer′s, cardiovascular disease, and diabetes. Oxidative stress can also lead to cancer formation by promoting tumor development and progression. Antioxidants derived from Lamiaceae plants play an important role in natural medicine, pharmacology, cosmetology, and aromatherapy. Herein, we examine the antioxidative capacity of essential oils from seven aromatic Lamiaceae plants against the synthetic radicals DPPH and ABTS. Among the essential oils analyzed, the most robust scavenging capacities were found in mixtures of volatile compounds from thyme and savory. The scavenging activity of tested EOs against the ABTS radical was clearly higher than activity towards DPPH. Analysis of essential oils with weaker antioxidant activity has shown that volatile compounds from marjoram, sage, and hyssop were more active than EOs from lavender and mint. It can be suggested that the potent antioxidant capacity of thyme (Thymus vulgaris) and savory (Satyreja hortensis) are related to a high level of phenolic constituents, such as thymol and carvacrol. On the other hand, the elevated antioxidative power of marjoram, sage, and hyssop essential oils may also be due to their terpinene, o-cymene, terpinolene, and terpinen-4-ol constituents. Although non-phenolic components are less active than thymol or carvacrol, they may affect antioxidant capacity synergistically.

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Production of secondary metabolites in intact plants and cultured cells of Thymus vulgaris L.

Planta Medica ◽

10.1055/s-0030-1264616 ◽

2010 ◽

Vol 76 (12) ◽

Author(s):

M Shams Ardekani ◽

A Hadjiakhoondi ◽

A Jamshidi ◽

N Forouzideh ◽

M Tavakolizadeh

Keyword(s):

Secondary Metabolites ◽

Cultured Cells ◽

Thymus Vulgaris ◽

Intact Plants

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IDENTIFICATION OF AROMA COMPOUNDS OF DRIED AND FRESH THYME (THYMUS VULGARIS L.) BY GAS CHROMATOGRAPHY-OLFACTOMETRY AND GAS CHROMATOGRAPHY-MASS SPECTROSCOPY ANALYSIS

Latin American Applied Research - An international journal ◽

10.52292/j.laar.2021.198 ◽

2020 ◽

Vol 51 (1) ◽

pp. 51-55

Author(s):

Gulcin Yildiz ◽

Ginnefer Cox ◽

Linda Moran

Keyword(s):

Gas Chromatography ◽

Volatile Compounds ◽

Mass Spectroscopy ◽

Aroma Compounds ◽

Lower Amount ◽

Thymus Vulgaris ◽

Volatile Components ◽

Drying Methods ◽

Gas Chromatography Olfactometry ◽

Gas Chromatography Mass Spectroscopy

This study was conducted to determine aroma compounds of oven-dried thyme and fresh thyme and investigate the drying effect in key aroma compounds of thyme. Fresh thyme was purchased and utilized for two functions. The first function was for the application of drying methods (fresh thyme was oven dried at 50°C), and the second function to analyze the fresh thyme. After applying solvent direct extraction, volatile compounds were isolated. According to Gas Chromatography-Olfactometry (GC-O) and Gas Chromatography-Mass Spectroscopy (GC-MS) analysis results, lower amount of volatile compounds for dried thyme were identified, while more volatile components were present in fresh thyme.

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Influence of Drying and Irradiation on the Composition of Volatile Compounds of Thyme (Thymus vulgaris L.)

Flavour and Fragrance Journal ◽

10.1002/(sici)1099-1026(199603)11:2<123::aid-ffj555>3.0.co;2-1 ◽

1996 ◽

Vol 11 (2) ◽

pp. 123-128 ◽

Cited By ~ 42

Author(s):

Rimantas Venskutonis ◽

Leif Poll ◽

Mette Larsen

Keyword(s):

Volatile Compounds ◽

Thymus Vulgaris

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Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053711 ◽

1982 ◽

Vol 40 ◽

pp. 210-211

Author(s):

M.J. Murphy ◽

R.R. Price ◽

J.C. Sloman

Keyword(s):

Cultured Cells ◽

Human Tumor ◽

Cell Type ◽

Tumor Mass ◽

Initial Cell ◽

Cloning Assay ◽

Human Tumor Cloning ◽

The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

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Electron Probe Microanalysis of Frozen Hydrated Kidneys, Isolated Cells, and Cultured Cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100054595 ◽

1982 ◽

Vol 40 ◽

pp. 402-403 ◽

Cited By ~ 1

Author(s):

Claude Lechene

Keyword(s):

Liquid Nitrogen ◽

Electron Probe Microanalysis ◽

Electron Probe ◽

Cultured Cells ◽

Liquid Nitrogen Temperature ◽

Elemental Content ◽

Isolated Cells ◽

Renal Tubular Cells ◽

Diamond Saw ◽

Saw Blade

Electron probe microanalysis of frozen hydrated kidneysThe goal of the method is to measure on the same preparation the chemical elemental content of the renal luminal tubular fluid and of the surrounding renal tubular cells. The following method has been developed. Rat kidneys are quenched in solid nitrogen. They are trimmed under liquid nitrogen and mounted in a copper holder using a conductive medium. Under liquid nitrogen, a flat surface is exposed by sawing with a diamond saw blade at constant speed and constant pressure using a custom-built cryosaw. Transfer into the electron probe column (Cameca, MBX) is made using a simple transfer device maintaining the sample under liquid nitrogen in an interlock chamber mounted on the electron probe column. After the liquid nitrogen is evaporated by creating a vacuum, the sample is pushed into the special stage of the instrument. The sample is maintained at close to liquid nitrogen temperature by circulation of liquid nitrogen in the special stage.

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The Infection of Cells by Bullet-Shaped Viruses

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100063779 ◽

1969 ◽

Vol 27 ◽

pp. 372-373

Author(s):

Frederick A. Murphy ◽

Alyne K. Harrison ◽

Sylvia G. Whitfield

Keyword(s):

Electron Microscopy ◽

Physical Properties ◽

Host Cell ◽

Thin Section ◽

Cultured Cells ◽

Cell Infection ◽

Thin Section Electron Microscopy ◽

Section Electron ◽

Section Electron Microscopy

The bullet-shaped viruses are currently classified together on the basis of similarities in virion morphology and physical properties. Biologically and ecologically the member viruses are extremely diverse. In searching for further bases for making comparisons of these agents, the nature of host cell infection, both in vivo and in cultured cells, has been explored by thin-section electron microscopy.

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Ultrastructure of two neoplasms in culture compared with original biopsies

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100110623 ◽

1984 ◽

Vol 42 ◽

pp. 50-51

Author(s):

Joseph M. Harb ◽

James T. Casper ◽

Vlcki Piaskowski

Keyword(s):

Electron Microscopy ◽

Tissue Culture ◽

Biopsy Specimen ◽

Cultured Cells ◽

Light And Electron Microscopy ◽

Human Tumor ◽

Soft Agar ◽

Human Tumor Cells ◽

Morphological Distinction ◽

Tumor Types

The application of tissue culture and the newer methodologies of direct cloning and colony formation of human tumor cells in soft agar hold promise as valuable modalities for a variety of diagnostic studies, which include morphological distinction between tumor types by electron microscopy (EM). We present here two cases in which cells in culture expressed distinct morphological features not apparent in the original biopsy specimen. Evaluation of the original biopsies by light and electron microscopy indicated both neoplasms to be undifferentiated sarcomas. Colonies of cells propagated in soft agar displayed features of rhabdomyoblasts in one case, and cultured cells of the second biopsy expressed features of Ewing's sarcoma.

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