scholarly journals 10. The Effect of Chemically-Synthesized Silver Nanoparticles on Performance and the Histology and Microbiological Profile of the Jejunum in Chickens

2016 ◽  
Vol 16 (2) ◽  
pp. 439-450 ◽  
Author(s):  
Katarzyna Ognik ◽  
Iwona Sembratowicz ◽  
Ewelina Cholewińska ◽  
Łukasz Wlazło ◽  
Bożena Nowakowicz-Dębek ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Growth Performance ◽  
Digestive Tract ◽  
Silver Nanoparticle ◽  
Broiler Chickens ◽  
Aerobic Bacteria ◽  
Selective Effect ◽  
Group Iii ◽  
Microbiological Profile ◽  
Microbiological Status

Abstract The aim of the study was to analyse how per os application of hydrocolloids of silver nanoparticles (22 nm) and lipid-coated nanosilver hydrocolloids (5 nm) affect the microbiological status and morphology of the jejunum of broiler chickens and their growth performance. The experiment was conducted on 60 chickens. The first group was the control. The chickens in group II received a silver nanoparticle hydrocolloid (Ag-nano) at a dose of 5 mg/kg b.w./day. The chickens in group III received a lipid-coated nanosilver hydrocolloid (AgL-nano) at a dose of 5 mg/kg b.w./day. Samples of digesta were taken from the jejunum during dissection and the total numbers of fungi, aerobic bacteria and bacteria of the coli group were determined in the samples. Samples of the jejunum were also collected during dissection to determine the length of the villi and depth of the crypts. The silver nanoparticles had no effect on growth performance or the histological picture of the jejunum. An increase was noted in the total number of aerobic mesophilic bacteria and a decrease in the number of coli group bacteria, which are facultative anaerobes, which indicates that the nanosilver had a selective effect on the microflora of the digestive tract in the chickens.

Effects of Compound Probiotics on Growth Performance, Intestinal Microflora and Immune Function of Broilers

2014 ◽  
Vol 997 ◽  
pp. 178-184
Author(s):  
Hong Ling Wang ◽  
Yu Hong Yang ◽  
Jia Qing Wang ◽  
Zhuo Wang ◽  
Shuang Ma
Keyword(s):  
Immune Function ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Intestinal Microflora ◽  
Basal Diet ◽  
Experimental Period ◽  
Control Group ◽  
Group Iii ◽  
Serum Biochemical ◽  
Anti Body

This study aimed to investigate the efficacy of compound probiotics on broiler performance, intestinal microflora, immune function and serum biochemical indicators. Four hundred one-d-old female broiler chickens were randomly divided into 4 groups with 4 replicates in each group and 25 chickens per replication. The chickens in groupI(control group) were fed a basal diet, experimental groups II, III and IVwere fed the basal diet supplemented with 0.1%, 0.2% and 0.4% compound probiotics. The results of this work were as follows: (1) during the experimental period, compared with control group, the average daily in group III was significantly increased (P<0.05), the feed/gain and average daily feed intake were significantly lower (P<0.05); (2) at the age of 14 and 28 days, the number of cecal Lactic acid in group II and III were significantly higher than the control (P<0.05) and the content of E.coli has the opposite results. (3) the anti-body titer in group III was the highest, and significantly higher compared to the control (P<0.05), but there were no significantly differences between group IV and control group (P>0.05). (4) serum biochemical indexes of broilers in group III and IVwere significantly increased than that in control group (P<0.05). In conclusion, the supplementation of 0.2% compound probiotics could promote the growth performance, improve the cecal mico-ecosystem, enhance the anti-body titer and biochemical indexes in serum.

The effect of a probiotic preparation containing Enterococcus faecium DSM 7134 for chickens on growth performance, immune status, and the histology and microbiological profile of the jejunum

2019 ◽  
Vol 59 (1) ◽  
pp. 101 ◽  
Author(s):  
Katarzyna Ognik ◽  
Ewelina Cholewińska ◽  
Magdalena Krauze ◽  
Katarzyna Abramowicz ◽  
Paulius Matusevicius
Keyword(s):  
Growth Performance ◽  
Enterococcus Faecium ◽  
Immune Status ◽  
Coliform Bacteria ◽  
Aerobic Bacteria ◽  
Feed Conversion ◽  
Microbiological Profile ◽  
Probiotic Preparation ◽  
Fattening Period ◽  
Histological Testing

The aim of the study was to determine whether and in what dosage and duration of administration a probiotic containing live cultures of Enterococcus faecium, enriched with vitamin D3 and ascorbic acid, affects the histological and microbiological profile of the intestine, as well as the immunity and growth performance of chickens. The jejunum together with its contents was also collected for microbiological and histological testing. The results obtained indicate that the birds receiving the probiotic preparation in the amount of 0.25 g/L of water during the entire fattening period attained significantly higher bodyweight than the Control, with significantly lower feed conversion. Furthermore, this dosage and duration was most efficient in reducing the total count of fungi, aerobic bacteria and coliform bacteria in the intestinal contents, in addition to increasing the height of the jejunal villi and depth of the crypts. The probiotic administered in the amount of 0.25 g/L of water during the entire fattening period also significantly increased lysozyme activity and the content of IgA in the blood serum as compared with the Control, while decreasing the content of IL-6, and thus most efficiently stimulated non-specific immunity in the chickens.

scholarly journals Performance, Intestinal Microbial Population, Immune and Physiological Responses of Broiler Chickens to Diet With Different Levels of Silver Nanoparticles Coated on Zeolite

2020 ◽  
Author(s):  
Niloofar Bolandi ◽  
Seyed Reza Hashemi ◽  
Daryoush Davoodi ◽  
Behrouz Dastar ◽  
Saeed Hassani ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibody Response ◽  
Silver Nanoparticle ◽  
Broiler Chickens ◽  
Control Diet ◽  
Anaerobic Bacteria ◽  
Control Group ◽  
Feed Conversion ◽  
Lymphocyte Ratio ◽  
Different Levels

Abstract Background: The aim of this study was to evaluate the effects of different levels of silver nanoparticles coated on zeolite on performance, gastrointestinal microflora, heterophile to lymphocyte ratio and humoral immune system responses of broiler chickens. Three hundred seventy five Cobb 500 in a completely randomized design were distributed to 5 experimental diets: control diet, 1% zeolite, 0.25%, 0.50% and 0.75% of silver nanoparticles coated on 1% zeolite. Performance traits were recorded and Blood samples were taken to determine antibody response against Newcastle on d 14, infectious bursal disease (Gumboro) on d 21 and Serum immunoglobulins (IgY, IgM, and IgA) were determined on d 42. Heterophile to lymphocyte ratio was evaluated on days 28, 35 and 42. At the end of d 21 and d 42. Results: The result showed that feed conversion ratio was significantly improved in silver nanoparticle treatments compare with the control group (p < 0.05). Total anaerobic bacteria and the population of E.coli in ileum and cecum were decreased (p < 0.05). The highest number of lactobacillus was observed in NS50 (p < 0.05). Antibody response against Gumboro was decreased as well as the amount of IgY antibody in silver nanoparticles treatments. Significant differences were observed in heterophile to lymphocyte ratio on d 28 and 35 and the highest amount was observed in NS50 group (p < 0.05). Conclusions: In conclusion, using silver nanoparticle dietary coated on zeolite could improve gut microflora, but silver nanoparticles could also have harmful side effects on the immune mechanism of broiler chickens.

scholarly journals Dietary supplementation of Agaricus bisporus stalk meal on growth performance, carcass and organ traits, meat quality, cecum mesophilic aerobic bacteria counts and intestinal histology in broiler chickens

2022 ◽  
Vol 52 (6) ◽  
Author(s):  
Aydin Altop ◽  
Isa Coskun ◽  
Ayse Gul Filik ◽  
Huseyin Cayan ◽  
Ahmet Sahin ◽  
...  
Keyword(s):  
Growth Performance ◽  
Meat Quality ◽  
Agaricus Bisporus ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Intestinal Morphology ◽  
Aerobic Bacteria ◽  
Breast Meat ◽  
Villus Height ◽  
Crypt Depth

ABSTRACT: Effects of dietary Agaricus bisporus mixture or stalk or cap on growth performance, carcass components and some meat quality parameters, mesophilic aerobic bacterial counts, and intestinal histomorphology in broiler chickens were investigated. Two hundred and forty one-day-old male Ross 308 broiler chickens were divided into 4 experimental groups with 4 replicates, each including 15 birds. Chickens were fed with basal diet (C), mushroom mixture (MM, 10 g stalk+10 g cap/kg diet), mushroom stalk (MS, 20 g/kg diet) and mushroom cap (MC, 20 g/kg diet). Feed conversion ratio (FCR) was improved (P < 0.01) by dietary MS while feed intake (FI) decreased (P < 0.01) in all treatment groups compared to control. However, body weight gain (BWG) was decreased (P < 0.01) by MC inclusion. Mushroom supplemented groups had higher (P < 0.05) mesophilic aerobic bacteria in the cecum. MS inclusion increased villus height to crypt depth ratio in the jejunum (P < 0.01) and villus height in the ileum (P < 0.01) and jejunum (P < 0.05). MC increased (P < 0.01) crypt depth in the jejunum. There were no statistical differences among groups for carcass components (P > 0.05). The L* values were decreased (P < 0.01) in thigh meat but increased (P < 0.01) in breast meat by mushroom inclusion. The a* values were decreased (P < 0.01) and b* values were increased (P < 0.05) in both thigh and breast meat with mushroom addition to diet. The obtained results indicated that A. bisporus stalk meal at an inclusion level of 20 g/kg of diet had favourable effects on growth performance with higher feed efficiency, improved intestinal morphology with higher villus height and increased meat quality of broiler chickens.

scholarly journals Effect on Growth Performance of Inoculation of Ruminococcus albus and Staphylococcus warneri into the Digestive Tract in Broiler Chickens

1991 ◽  
Vol 62 (11) ◽  
pp. 1065-1068
Author(s):  
Tatsuo MURAMATSU ◽  
Nobuhiro NIWA ◽  
Naomi MORIYAMA ◽  
Mitsuhiro FURUSE ◽  
Junichi OKUMURA ◽  
...  
Keyword(s):  
Growth Performance ◽  
Digestive Tract ◽  
Broiler Chickens ◽  
Ruminococcus Albus ◽  
Staphylococcus Warneri ◽  
Effect On Growth

Effect of silver nanoparticles on growth performance, metabolism and microbial profile of broiler chickens

2012 ◽  
Vol 66 (5) ◽  
pp. 416-429 ◽  
Author(s):  
Lane Pineda ◽  
Andrè Chwalibog ◽  
Ewa Sawosz ◽  
Charlotte Lauridsen ◽  
Ricarda Engberg ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Microbial Profile

Effects of feeding Isomaltooligosaccharides on the growth performance, carcass traits and immune response of broiler chickens

2014 ◽  
Vol 3 (1) ◽  
pp. 150-157
Author(s):  
Khalid M. Gaafar
Keyword(s):  
Immune Response ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Carcass Traits ◽  
Dietary Supplementation ◽  
Control Group ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Flu Vaccine ◽  
Lipids Profile

The research was conducted to study the effect of feeding broiler chickens on diets containing isomaltooligosaccharides on the growth performance, carcass traits and immune response. 90-one day old broiler chicks were used according to completely randomized two treatment groups and one control, 30 birds each. Birds fed ad-libitum on basal starter and grower-finisher diets for 35 day. Diets of treatment`s groups contained 0.5 g/Kg and 1 g/Kg of Isomaltooligosaccharides, while the control group fed on the basal diets without Isomaltooligosaccharides supplementation. Dietary supplementation of broiler chickens with Isomaltooligosaccharides improved body weight, feed conversion, carcass traits, two lymphoid organs weight and log antibody titer against avian flu vaccine. Most of the highest values were for birds fed low levels of Isomaltooligosaccharides. Feed intake decreases as Isomaltooligosaccharides level increases. Dietary supplementation with Isomaltooligosaccharides did not affect the lipids profile (triglycerides, total cholesterol, LDL and HDL), however the blood VLDL levels decreased with increased levels of Malondialdehyde and Glutathione reductase. Collectively, Dietary supplementation of broiler chickens with 0.5 g/Kg diet of Isomaltooligosaccharides improved growth performance, carcass traits and immune status.

Detection of intracellular silver nanoparticles using flow cytometry

2018 ◽  
pp. 223-226
Author(s):  
С.И. Каба ◽  
А.А. Соколовская
Keyword(s):  
Flow Cytometry ◽  
Silver Nanoparticles ◽  
Endothelial Cells ◽  
Silver Nanoparticle ◽  
Cell Uptake ◽  
Control Samples

Продемонстрировано обнаружение наночастиц серебра во внутриклеточном пространстве с помощью проточной цитофлуориметрии. В эндотелиальных клетках линии EA.hy926, инкубированных в растворе, содержащем 2 мкг/мл наносеребра, измеряли боковое светорассеяние. По сравнению с контрольными образцами этот параметр возрастал, в то время как прочие значимые характеристики не изменялись. Это подтверждает чувствительность метода к изменившемуся состоянию клеток и указывает на поглощение наночастиц серебра клетками при концентрации ниже токсической. The study demonstrated a possibility for detection of intracellular silver nanoparticles using flow cytometry. The parameter used in this work, side scattering, was measured in EA.hy926 endothelial cells incubated in a 2 mg/ml silver nanoparticle solution. This parameter was increased compared to control samples. Therefore, this technique was sensitive to changes in the cell status and suggested the cell uptake of the particles under the subtoxic conditions.

Sign in / Sign up

Export Citation Format

Share Document