The effect of a probiotic preparation containing Enterococcus faecium DSM 7134 for chickens on growth performance, immune status, and the histology and microbiological profile of the jejunum

2019 ◽  
Vol 59 (1) ◽  
pp. 101 ◽  
Author(s):  
Katarzyna Ognik ◽  
Ewelina Cholewińska ◽  
Magdalena Krauze ◽  
Katarzyna Abramowicz ◽  
Paulius Matusevicius
Keyword(s):  
Growth Performance ◽  
Enterococcus Faecium ◽  
Immune Status ◽  
Coliform Bacteria ◽  
Aerobic Bacteria ◽  
Feed Conversion ◽  
Microbiological Profile ◽  
Probiotic Preparation ◽  
Fattening Period ◽  
Histological Testing

The aim of the study was to determine whether and in what dosage and duration of administration a probiotic containing live cultures of Enterococcus faecium, enriched with vitamin D3 and ascorbic acid, affects the histological and microbiological profile of the intestine, as well as the immunity and growth performance of chickens. The jejunum together with its contents was also collected for microbiological and histological testing. The results obtained indicate that the birds receiving the probiotic preparation in the amount of 0.25 g/L of water during the entire fattening period attained significantly higher bodyweight than the Control, with significantly lower feed conversion. Furthermore, this dosage and duration was most efficient in reducing the total count of fungi, aerobic bacteria and coliform bacteria in the intestinal contents, in addition to increasing the height of the jejunal villi and depth of the crypts. The probiotic administered in the amount of 0.25 g/L of water during the entire fattening period also significantly increased lysozyme activity and the content of IgA in the blood serum as compared with the Control, while decreasing the content of IL-6, and thus most efficiently stimulated non-specific immunity in the chickens.

Use of Bacillus subtilis PB6 enriched with choline to improve growth performance, immune status, histological parameters and intestinal microbiota of broiler chickens

2020 ◽  
Vol 60 (5) ◽  
pp. 625 ◽  
Author(s):  
Katarzyna Abramowicz ◽  
Magdalena Krauze ◽  
Katarzyna Ognik
Keyword(s):  
Bacillus Subtilis ◽  
Beneficial Effect ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Immune Status ◽  
Coliform Bacteria ◽  
Phagocytic Index ◽  
Control Group ◽  
Probiotic Preparation ◽  
Poultry Nutrition

Context Probiotics used in poultry nutrition may promote the propagation of beneficial bacteria and limit the growth of pathogens, improving the body’s resistance to disease. Aims The aim of the study was to establish the dose and timing of administration of a probiotic preparation containing live cultures of Bacillus subtilis PB6 and choline that would have the most beneficial effect on histological parameters and microbiological status of the intestine, immune status, and growth performance of broiler chickens. Methods In total, 980 one-day-old Ross 308 male chickens, divided into seven groups of 140 (each group with seven replications of 20 individuals), were used in the experiment. The birds were reared until Day 42 of life. In the control group, birds received water without addition of the probiotic preparation. In three continuous treatments, birds received the probiotic preparation in their water at doses of 0.05, 0.1 or 0.25 g/L from Day 1 to Day 42 of rearing. In three periodic treatments, birds received the same doses of the probiotic preparation, but only during Days 1–7, 15–21 and 29–35 of rearing. Key results Addition of the probiotic preparation to the drinking water of broiler chickens increased the total number of aerobic bacteria as well as the length of the intestinal villi and the depth of the crypts. It also reduced the number of fungi and coliform bacteria. In addition, increases were noted in the lysozyme content, the phagocytic index, and the level of immunoglobulin A. Conclusions The probiotic preparation containing B. subtilis and choline administered continuously at a dose of 0.25 g/L in water throughout the rearing period had the most beneficial effect on the composition of the microbiome, and improved the mucous membrane structure of the intestine (increasing villus length and intestinal crypt depth), immunity, and growth performance of the broiler chickens. Implications The use of probiotics in poultry nutrition improves the health of animals and makes breeding more profitable. Administration of this probiotic improves disease resistance, jejunal histological parameters, and intestinal microflora of broiler chickens.

Combined administration of bacteriocin- -producing, probiotic strain Enterococcus faecium CCM7420 with Eleutherococcus senticosus and their effect in rabbits

2013 ◽  
Vol 16 (4) ◽  
pp. 619-627 ◽  
Author(s):  
M. Pogány Simonová ◽  
A. Lauková ◽  
L. Chrastinová ◽  
I. Plachá ◽  
V. Strompfová ◽  
...  
Keyword(s):  
Growth Performance ◽  
Enterococcus Faecium ◽  
Average Daily Gain ◽  
Probiotic Strain ◽  
Cholesterol Concentration ◽  
Control Group ◽  
Feed Conversion ◽  
Eleutherococcus Senticosus ◽  
Commercial Diet ◽  
And Control

Abstract The effect of Enterococcus faecium CCM7420 (EF) - enterocin-producing and probiotic strain of rabbit origin, Eleutherococcus senticosus extract (ES) and their combination (ES+EF) was determined on selected bacteria in faeces and caecum content, leukocytes phagocytosis, blood biochemistry and growth performance. Ninety-six weaned rabbits were divided into 3 experimental (ES, EF, ES+EF) and control group (CG). The rabbits in the groups ES and EF+ES were fed commercial diet enriched with E. senticosus extract (30 g/100 kg feed), rabbits in groups EF and CG were fed untreated diet. The rabbits in the EF and ES+EF groups were administered with an overnight culture of E. faecium CCM7420 strain (500 μl/animal/day into water, 109 CFU/ml). The treatment period lasted 21 days. The microbiological examinations in faecal samples confirmed the presence of E. faecium CCM7420 strain. In groups EF and ES+EF, the reduction of faecal coliforms, Pseudomonas-like sp., Clostridium-like sp. and S. aureus was recorded. Leucocyte phagocytosis significantly increased in all experimental groups (P<0.0001) compared to CG. The lowest GPx values were measured in the ES+EF group. Higher total protein, triglycerides and calcium concentrations were detected in experimental groups compared to CG. The cholesterol concentration decreased in the ES group. The highest average daily gain was recorded in EF group; in ES+EF the better feed conversion ratio and no mortality was recorded. These results indicated that the dietary supplementation with the E. faecium CCM7420 and E. senticosus extract stimulate the leukocytes phagocytosis and reduces the potential pathogens in rabbits digestive tract without oxidative stress and improve the growth performance.

scholarly journals 10. The Effect of Chemically-Synthesized Silver Nanoparticles on Performance and the Histology and Microbiological Profile of the Jejunum in Chickens

2016 ◽  
Vol 16 (2) ◽  
pp. 439-450 ◽  
Author(s):  
Katarzyna Ognik ◽  
Iwona Sembratowicz ◽  
Ewelina Cholewińska ◽  
Łukasz Wlazło ◽  
Bożena Nowakowicz-Dębek ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Growth Performance ◽  
Digestive Tract ◽  
Silver Nanoparticle ◽  
Broiler Chickens ◽  
Aerobic Bacteria ◽  
Selective Effect ◽  
Group Iii ◽  
Microbiological Profile ◽  
Microbiological Status

Abstract The aim of the study was to analyse how per os application of hydrocolloids of silver nanoparticles (22 nm) and lipid-coated nanosilver hydrocolloids (5 nm) affect the microbiological status and morphology of the jejunum of broiler chickens and their growth performance. The experiment was conducted on 60 chickens. The first group was the control. The chickens in group II received a silver nanoparticle hydrocolloid (Ag-nano) at a dose of 5 mg/kg b.w./day. The chickens in group III received a lipid-coated nanosilver hydrocolloid (AgL-nano) at a dose of 5 mg/kg b.w./day. Samples of digesta were taken from the jejunum during dissection and the total numbers of fungi, aerobic bacteria and bacteria of the coli group were determined in the samples. Samples of the jejunum were also collected during dissection to determine the length of the villi and depth of the crypts. The silver nanoparticles had no effect on growth performance or the histological picture of the jejunum. An increase was noted in the total number of aerobic mesophilic bacteria and a decrease in the number of coli group bacteria, which are facultative anaerobes, which indicates that the nanosilver had a selective effect on the microflora of the digestive tract in the chickens.

scholarly journals Characteristics of steers of six beef breeds fattened from eight months of age and slaughtered at a target level of intramuscular fat – I. Growth Performance and carcass quality

2001 ◽  
Vol 44 (4) ◽  
pp. 395-412 ◽  
Author(s):  
A. Chambaz ◽  
I. Morel ◽  
M. R. L. Scheeder ◽  
M. Kreuzer ◽  
P.-A. Dufey
Keyword(s):  
Growth Performance ◽  
Intramuscular Fat ◽  
Housing System ◽  
Carcass Quality ◽  
Target Level ◽  
Feed Conversion ◽  
In Series ◽  
Fattening Period ◽  
Imf Content

Abstract. Growth Performance and carcass quality of 132 steers originating from six beef breeds, Angus (AN), Simmental (SI), Charolais (CH), Limousin (LI), Blonde d'Aquitaine (BL), and Piedmontese (PI), fattened under the same conditions on the same diet, were compared at a target level of 3.5 % intramuscular fat (IMF) in the M. longissimus dorsi. This target level was set on basis of the results ofa preliminary study investigating, with 784 persons, the Visual preference of marbling using photographs. The total mix ration, provided at ad libitum access, consisted of maize silage, grass silage and concentrate (52 %, 26 % and 22 % of DM, resp.). Series 1 was performed in a tie-stall bam while a loose-housing system with straw bedding was used in series 2. The animals were assigned to slaughter either when the target IMF content was reached according to the estimation with a real-time ultrasound system applied in the live animals or when 15 months of fattening had passed. AN, SI, CH and LI reached 3.5 % IMF on average at final weights of 501 ± 43, 628 ± 60, 693 ± 117 and 668 ± 65 kg, respectively. BL and PI did not reach this target, although the average fattening period was about three times longer for BL and PI than for AN and the final weights were 758 ± 93 and 647 ± 64 kg, respectively. Under the conditions of this experimental approach, daily gains were highest in AN, followed by CH, SI, LI and BL and lowest in PI. The daily feed intake was significantly lower for PI than for CH, SI and AN. The AN expressed the best feed conversion efficiency in terms of DM expenditure per kg gain over the complete fattening period while this efficiency was lowest in the PI group followed by BL. Among the four breeds, which reached the target IMF content, LI steers showed the greatest proportion of premium cuts and the highest lean to fat and lean to bone ratio in the sirloin, followed, in descending order, by CH, SI and AN. However all four groups were graded around 4 in fatness score (high to very high). The present results revealed for all breeds the difficulty to reach the desired extent of marbling and at the same time favourable carcass conformation, carcass size (except AN) and fat cover which meet market demands.

scholarly journals Single and Combined Effects of Clostridium butyricum and Saccharomyces cerevisiae on Growth Indices, Intestinal Health, and Immunity of Broilers

Animals ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 184 ◽  
Author(s):  
Mervat Abdel-Latif ◽  
Mohamed Abd El-Hack ◽  
Ayman Swelum ◽  
Islam Saadeldin ◽  
Ahmed Elbestawy ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Performance ◽  
Immune Status ◽  
Body Weight Gain ◽  
Basal Diet ◽  
Clostridium Butyricum ◽  
Gut Health ◽  
Intestinal Health ◽  
Feed Conversion ◽  
Crypt Depth

A total of 120 1-day-old commercial Cobb chicks were used to study the effects of Clostridium butyricum (C. butyricum) and/or Saccharomyces cerevisiae (S. cerevisiae) on growth performance, intestinal health, and immune status in broilers. The experimental groups were as follows: G1; basal diet (BD), G2; basal diet (BD) plus C. butyricum preparation at 0.5 g/kg diet, G3; BD plus S. cerevisiae preparation at 0.5 g/kg diet, G4; BD plus 0.25 g/kg C. butyricum preparation plus 0.25 g/kg S. cerevisiae. Results showed that the total body weight gain, feed conversion efficiency, and protein efficiency ratio were significantly higher (p < 0.05) in the G4 group than in the other groups. The mortality percentage was reduced in the probiotic-supplemented groups. The villi height was elongated, and the villus height/crypt depth ratio was significantly increased in G2 and G4 chicks, compared to those in the control. The crypt depth was significantly decreased in all the probiotic-supplemented groups. Hemagglutination inhibition titers for Newcastle disease virus (NDV) were markedly increased in G2 and G4 chicks at 35 days of age, compared to those in G3 and control chicks. These results showed that dietary supplementation of a combined mixture of C. butyricum and S. cerevisiae in an equal ratio (G4) was more effective in improving growth performance, immune status, and gut health of broilers, compared with individual supplementation at a full dose.

Effects of feeding Isomaltooligosaccharides on the growth performance, carcass traits and immune response of broiler chickens

2014 ◽  
Vol 3 (1) ◽  
pp. 150-157
Author(s):  
Khalid M. Gaafar
Keyword(s):  
Immune Response ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Carcass Traits ◽  
Dietary Supplementation ◽  
Control Group ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Flu Vaccine ◽  
Lipids Profile

The research was conducted to study the effect of feeding broiler chickens on diets containing isomaltooligosaccharides on the growth performance, carcass traits and immune response. 90-one day old broiler chicks were used according to completely randomized two treatment groups and one control, 30 birds each. Birds fed ad-libitum on basal starter and grower-finisher diets for 35 day. Diets of treatment`s groups contained 0.5 g/Kg and 1 g/Kg of Isomaltooligosaccharides, while the control group fed on the basal diets without Isomaltooligosaccharides supplementation. Dietary supplementation of broiler chickens with Isomaltooligosaccharides improved body weight, feed conversion, carcass traits, two lymphoid organs weight and log antibody titer against avian flu vaccine. Most of the highest values were for birds fed low levels of Isomaltooligosaccharides. Feed intake decreases as Isomaltooligosaccharides level increases. Dietary supplementation with Isomaltooligosaccharides did not affect the lipids profile (triglycerides, total cholesterol, LDL and HDL), however the blood VLDL levels decreased with increased levels of Malondialdehyde and Glutathione reductase. Collectively, Dietary supplementation of broiler chickens with 0.5 g/Kg diet of Isomaltooligosaccharides improved growth performance, carcass traits and immune status.

scholarly journals Effects of Different Swimming Pool Conditions and Floor Types on Growth Performance and Footpad Dermatitis in Indoor-Reared White Roman Geese

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1705
Author(s):  
Shih-Chieh Liao ◽  
Pei-Xuan Lu ◽  
Shih-Yi Shen ◽  
Chih-Chang Hsiao ◽  
Ching-Yi Lien ◽  
...  
Keyword(s):  
Growth Performance ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Swimming Pool ◽  
Poultry Production ◽  
Performance Measurements ◽  
Feed Conversion ◽  
Plantar Surface ◽  
Rearing Conditions ◽  
Foot Disease

Footpad dermatitis (FPD) is a major foot disease in modern poultry production, and it affects both poultry health and animal welfare. It refers to inflammation and necrotizing lesions on the plantar surface of the footpads and toes. We investigated the effects of providing a swimming pool and different floor types on growth performance and FPD score in indoor-reared White Roman geese. Forty-eight male and 48 female White Roman geese were randomly allocated to pens with or without a swimming pool and with either mud or perforated plastic floor and reared from 15 to 84 days of age. Growth performance measurements included feed intake (FI), weight gain (WG), and feed conversion ratio (FCR). FI, WG, and FCR were significantly decreased at various growth periods in geese provided with a pool. Lower WG and bodyweight for the perforated plastic floor group were found at 15–28 and 28 days of age, respectively. The geese reared on the perforated plastic floors without a pool had higher FPD scores at 70 and 84 days of age than those with other rearing conditions. A higher incidence of FPD score 1 was observed in geese raised without a pool. In conclusion, providing a pool can improve footpad health in indoor-reared White Roman geese but may not benefit growth performance.

scholarly journals Supplementing Garlic Nanohydrogel Optimized Growth, Gastrointestinal Integrity and Economics and Ameliorated Necrotic Enteritis in Broiler Chickens Using a Clostridium perfringens Challenge Model

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 2027
Author(s):  
Doaa Ibrahim ◽  
Tamer Ahmed Ismail ◽  
Eman Khalifa ◽  
Shaimaa A. Abd El-Kader ◽  
Dalia Ibrahim Mohamed ◽  
...  
Keyword(s):  
Growth Performance ◽  
Clostridium Perfringens ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Economic Benefits ◽  
Production Costs ◽  
Broiler Chicks ◽  
Necrotic Enteritis ◽  
Feed Conversion ◽  
Intestinal Lesion

Necrotic enteritis (NE) caused by Clostridium perfringens (C. perfringens) results in impaired bird growth performance and increased production costs. Nanotechnology application in the poultry industry to control NE outbreaks is still not completely clarified. Therefore, the efficacy of dietary garlic nano-hydrogel (G-NHG) on broilers growth performance, intestinal integrity, economic returns and its potency to alleviate C. perfringens levels using NE challenge model were addressed. A total of 1200 male broiler chicks (Ross 308) were assigned into six groups; four supplemented with 100, 200, 300 or 400 mg of G-NHG/kg diet and co-challenged with C. perfringens at 21, 22 and 23 d of age and two control groups fed basal diet with or without C. perfringens challenge. Over the total growing period, the 400 mg/kg G-NHG group had the most improved body weight gain and feed conversion efficiency regardless of challenge. Parallel with these results, the mRNA expression of genes encoding digestive enzymes (alpha 2A amylase (AMY2A), pancreatic lipase (PNLIP) and cholecystokinin (CCK)) and intestinal barriers (junctional adhesion molecule-2 (JAM-2), occludin and mucin-2 (Muc-2)) were increased in groups fed G-NHG at higher levels to be nearly similar to those in the unchallenged group. At 14 d post challenge, real-time PCR results revealed that inclusion of G-NHG led to a dose-dependently decrease in the C. perfringens population, thereby decreasing the birds’ intestinal lesion score and mortality rates. Using 400 mg/kg of G-NHG remarkably ameliorated the adverse effects of NE caused by C. perfringens challenge, which contributed to better growth performance of challenged birds with rational economic benefits.

scholarly journals A Very Low CP Level Reduced Diarrhoea and Productivity in Weaner Pigs, but No Differences between Post-Weaning Diets Including Soybean Meal or Soy Protein Concentrate Were Found

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 678
Author(s):  
Julie C. Lynegaard ◽  
Niels J. Kjeldsen ◽  
Julie K. Bache ◽  
Nicolai R. Weber ◽  
Christian F. Hansen ◽  
...  
Keyword(s):  
Growth Performance ◽  
Soy Protein ◽  
Soybean Meal ◽  
Nutrient Utilization ◽  
Positive Control ◽  
Negative Control ◽  
Soy Protein Concentrate ◽  
Protein Concentrate ◽  
Feed Conversion ◽  
And Performance

Soy protein concentrate improves nutrient utilization and growth performance compared to soybean meal, and diets with a low crude protein (CP) level decreases diarrhoea. The objectives were to (1) test a low CP diet based on different soy products, and (2) to test a very-low CP diet (15.1%) with amino acids (AA) on diarrhoea and productivity. A total of 5,635 weaned pigs (~28 days), were assigned to five dietary treatments; PC (positive control): Standard CP levels (192, 189, 191 g/kg CP) with 2500 ppm ZnO; NC (negative control): Same as PC without ZnO; SP (Soy protein concentrate): Low CP levels (176, 174, 191 g/kg CP); SB (Soybean meal): Low CP levels (177, 176, 191 g/kg CP); and XLA (X-low CP + AA): Very low CP levels (154, 151, 191 g/kg CP) with AA. The PC and XLA diets reduced diarrhoea by 41 and 61%, respectively, compared to the NC group, while no difference between SB and SP were observed. The XLA diet reduced feed intake and daily gain compared with PC and NC, where SP, SB, and XLA had a poorer feed conversion compared with PC. Conclusively, the SP and SB low-protein diets did not reduce diarrhoea or growth performance, whereas the XLA diet decreased both diarrhoea and performance.

scholarly journals Effect of dietary supplementation of seaweed (Ulva lactuca) and Azolla on growth performance, haematological and serum biochemical parameters of Aseel chicken

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Vijayalingam Thavasi Alagan ◽  
Rajesh Nakulan Vatsala ◽  
Ilavarasan Sagadevan ◽  
Vairamuthu Subbiah ◽  
Venkataramanan Ragothaman
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Control Diet ◽  
Dietary Supplementation ◽  
Well Being ◽  
Ulva Lactuca ◽  
Feed Conversion ◽  
Serum Biochemical

Abstract Background An effort was made to assess the effectiveness of dietary supplementation of Ulva lactuca (U. lactuca), a seaweed and Azolla individually and in combination on different physiological parameters of Aseel chicken. A total of 40 Aseel chicks of 8 weeks old were allocated into four groups, C1 (control), T1 (Azolla alone), T2 (seaweed alone) and T3 (seaweed plus Azolla). Aseel chicks in C1 were fed with formulated grower feed alone, birds in T1 had control diet with 5% Azolla, birds in T2 had control diet with 3% U. lactuca and birds in T3 had control diet admixtured with 5% of Azolla and 3% of U. lactuca. Parameters were recorded for a continuous period of 2 months in 15 days interval. Results The growth performance was found to be significant (P ≤ 0.05) during 30 days of feed trials and highly significant (P ≤ 0.01) during 45 and 60 days of treatment. Birds in T3 had a higher body weight gain, shank length and feed conversion efficiency followed with T1 and T2 compared to C1. PCV, RBC, WBC and heterophil counts were not influenced (P ≥ 0.05) by dietary treatments. Uric acid, creatinine, AST, glucose, triglycerides and magnesium levels revealed a high significant (P ≤ 0.01) variation, and cholesterol level showed significant (P ≤ 0.05) changes during 30th and 60th day of feed trials between the control and treatment groups. Total protein, globulin, phosphorus and electrolytes like Na, K and Cl levels were not significant (P ≥ 0.05) during 30th day and were highly significant (P ≤ 0.01) during the 60th day of the trial. Conclusions It could be noted that inclusion of U. lactuca and Azolla as feed supplement in grower chicken had a better body weight gain when given in combination (U. lactuca and Azolla) rather than supplemented with the sole entity. Based on the haematological and serum biochemical analysis, the supplementation of U. lactuca and Azolla at the levels included in this trial did not pose any threat to the physiological well-being of Aseel chicken.

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