scholarly journals Comparative response of IgA and IgG activity and hematological parameters among four main beef-cattle breeds infected with gastrointestinal nematodes in the warm humid tropic of Mexico

2017 ◽  
Vol 17 (3) ◽  
pp. 819-833 ◽  
Author(s):  
Roberto González-Garduño ◽  
María E. López Arellano ◽  
Pedro Mendoza de Gives ◽  
Javier Arece García ◽  
Carine Marie-Magdeleine ◽  
...  
Keyword(s):  
Hematological Parameters ◽  
Immunoglobulin A ◽  
Gastrointestinal Nematodes ◽  
Tropical Region ◽  
Serum Samples ◽  
Brown Swiss ◽  
Breed Group ◽  
Comparative Response ◽  
Fixed Model ◽  
Leukocyte Counts

Abstract This study determined the immunoglobulin (A and G) activity against gastrointestinal nematodes (GIN) and also the hematological parameters in four beef-calf breeds naturally infected in a tropical region of Mexico. Thirty-six infected calves were used to determine the fecal nematode egg counts (FEC), the IgA and IgG activity in serum and saliva, the packed cell volume (PCV), the plasmatic protein (PP) and the differential leukocyte counts. These parameters were measured for a five-month period. ELISA assay was performed using adult worm crude antigen (AWCA) from Haemonchus contortus, Trichostrongylus colubriformis, Mecistocirrus digitatus and Cooperia punctata. The variables were analyzed using a fixed model according to the breed group. The FEC records (means ± standard deviation) for the different breeds were: Brangus (122±115), Charolais (391±507), Guzerat (294±326) and Brown Swiss (413±395). No statistical differences (P>0.05) were found among breeds. Cooperia and Haemonchus were the main identified genera in the coprocultures. Differences in hematological parameters were observed among breeds (P<0.01); Guzerat showed the highest PCV (42.6±4.7%). The lowest leukocyte counts were observed in the Charolais breed (9.2±2.4 × 109 cells L-1). Differences between Guzerat and Charolais were only detected in eosinophils, neutrophils and lymphocytes. Increased activity of IgA was observed against M. digitatus (21% OD) compared with the response against C. punctata in serum samples (13% OD). The lowest IgA activity in serum and saliva was found in Guzerat calves. Charolais and Brown Swiss showed the highest IgA activity in serum and the highest value in saliva was found in Brangus.

scholarly journals Evaluation of Anthelmintic Efficacy of Ivermectin, Levamisole HCl and Albendazole Administered through Different Routes against Naturally Occuring Gastrointestinal Nematodiasis in Black Bengal Goat Inducing Hematological Parameters and Live Weight Indices

2016 ◽  
Vol 5 (1) ◽  
pp. 26-34 ◽  
Author(s):  
M Aktaruzzaman ◽  
MS Islam ◽  
MMI Hasan ◽  
MJU Bhuiyan ◽  
MM Hossain ◽  
...  
Keyword(s):  
Body Weight ◽  
Hematological Parameters ◽  
Live Weight ◽  
Gastrointestinal Nematodes ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Counting Method ◽  
Blood Samples ◽  
Group A ◽  
Group D

This study aimed to evaluate the efficacy of ivermectin, levamisole HCl and albendazole against gastrointestinal nematodes in naturally infected goats of government goat development farm, Sylhet, Bangladesh. The study included 52 black Bengal breed of which 30 were naturally infected and randomly selected 20 on the basis of their weight and egg count. Twenty black Bengal goats of 12-13 month old irrespective of sex infested with gastrointestinal nematodes were selected for this experiment and randomly divided into four equal groups (group A, B, C and D) where each group consisted of 5 goats and goats of group D were kept as control group. One injectable ivermectin (200?gkg-1 body weight, S/C) preparations (Techno Drugs Ltd. Bangladesh) and two solid levamisole HCl, albendazole (7.5 mgkg-1 body weight, orally) preparations (The ACME Laboratories Ltd. and Square Pharmaceuticals Ltd. Bangladesh) were used for positive control of gastrointestinal nematodes as group A, B and C. Goats of group D was kept as control without giving any treatment. Before trials (day 0), total egg count, blood samples and initial body weight were recorded. During the study period the fecal and blood samples were collected directly from rectum and examined on 7th, 14th, 21st and 28th day using McMaster fecal egg counting method. Body weight was recorded on day 28 following the treatments. The results of the comparative efficacies of different anthelmintic of ivermectin was 86.75%, followed by levamisole HCl 85.07% and albendazole 92.81%. McMaster fecal egg counting method discloses the percentage of Haemonchus spp. (15.38%), with Trichostrongylus spp, Strongyloides spp., and Cooperia spp. also present. After treatment with ivermectin, levamisole HCl and albendazole, Total Erythrocyte Count (TEC), Hemoglobin (Hb) content and Packed Cell Volume (PCV) were increased significantly (p<0.01 and p>0.05) in goats but Erythrocyte Sedimentation Rate (ESR) and Total Leukocyte Count (TLC) were decreased significantly (p<0.05 and p>0.01) in all treated goat and body weight was increased significantly (p<0.01) on day 28.International Journal of Natural Sciences (2015), 5(1) 26-34

scholarly journals Immunoglobulin A Antibody Responses in Dengue Patients: a Useful Marker for Serodiagnosis of Dengue Virus Infection

2005 ◽  
Vol 12 (10) ◽  
pp. 1235-1237 ◽  
Author(s):  
M. Nawa ◽  
T. Takasaki ◽  
M. Ito ◽  
S. Inoue ◽  
K. Morita ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Immunoglobulin A ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Infections ◽  
Serum Samples ◽  
Dengue Virus Infection ◽  
Iga Antibody ◽  
Antibody Capture

ABSTRACT We determined the usefulness of an immunoglobulin A (IgA) antibody-capture enzyme-linked immunosorbent assay for serodiagnosis of dengue virus infections. The results indicate that the presence of IgA and IgM in serum samples assures recent primary dengue virus infection even with a single serum sample.

scholarly journals The latest FAD – Faecal antibody detection in cattle. Protocol and results from three UK beef farms naturally infected with gastrointestinal nematodes

Parasitology ◽  
2018 ◽  
Vol 146 (1) ◽  
pp. 89-96 ◽  
Author(s):  
A. S. Cooke ◽  
K. A. Watt ◽  
E. R. Morgan ◽  
J. A. J. Dungait
Keyword(s):  
Animal Health ◽  
Nematode Species ◽  
Gastrointestinal Nematodes ◽  
Vital Role ◽  
Antibody Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Fecal Samples ◽  
Gastrointestinal Health ◽  
Immunological Protection

AbstractAntibodies at gastrointestinal mucosal membranes play a vital role in immunological protection against a range of pathogens, including helminths. Gastrointestinal health is central to efficient livestock production, and such infections cause significant losses. Fecal samples were taken from 114 cattle, across three beef farms, with matched blood samples taken from 22 of those animals. To achieve fecal antibody detection, a novel fecal supernatant was extracted. Fecal supernatant and serum samples were then analysed, using adapted enzyme-linked immunosorbent assay protocols, for levels of total immunoglobulin (Ig)A, IgG, IgM, andTeladorsagia circumcincta-specific IgA, IgG, IgM and IgE (in the absence of reagents for cattle-specific nematode species). Fecal nematode egg counts were conducted on all fecal samples. Assays performed successfully and showed that IgA was the predominant antibody in fecal samples, whereas IgG was predominant in serum. Total IgA in feces and serum correlated within individuals (0.581,P= 0.005), but other Ig types did not. Results support the hypothesis that the tested protocols are an effective method for the non-invasive assessment of cattle immunology. The method could be used as part of animal health assessments, although further work is required to interpret the relationship between results and levels of infection and immunity.

scholarly journals Geographic Distribution of Ehrlichia canis TRP Genotypes in Brazil

2020 ◽  
Vol 7 (4) ◽  
pp. 165
Author(s):  
Isis Indaiara Gonçalves Granjeiro Taques ◽  
Amanda Noeli Silva Campos ◽  
Mayara Lima Kavasaki ◽  
Sayanne Luns Hatum de Almeida ◽  
Daniel Moura de Aguiar
Keyword(s):  
Synthetic Peptides ◽  
Costa Rican ◽  
Ehrlichia Canis ◽  
Dense Core ◽  
Tropical Region ◽  
The South ◽  
Serum Samples ◽  
Temperate Region ◽  
Repeat Proteins ◽  
Tandem Repeat Proteins

Tandem repeat proteins (TRPs) are major immunoreactive proteins of Ehrlichia canis, which have been used in the serological diagnosis of different genotypes of the microorganism. TRP19 is preserved among different E. canis isolates expressed on both reticulate and dense-core cells and observed in the extracellular matrix or associated with the morula membrane. TRP36 is differentially expressed only on the surface of the dense-core form of the bacterium and exhibits more divergence among isolates. The aim of this study was to evaluate the distribution of the American (USTRP36), Brazilian (BrTRP36) and Costa Rican (CRTRP36) genotypes of E. canis in Brazil, using ELISA assays. Serum samples of 814 dogs from 49 municipalities from all over Brazil were analyzed. Our results showed that 34% of the samples were reactive to the USTRP36 genotype and 32.6% to the BrTRP36 genotype. The two genotypes appeared to occur equally throughout Brazil, although the frequency of seropositivity was lower in the south than in the country’s other regions. Dogs that reacted to at least one of the synthetic peptides (TRP19 and TRP36) were 456 (56%). A few dogs (n = 5; 0.61%) reactive to the E. canis TRP36 genotype (CRTRP36) were also detected in the northeast and southern regions. We concluded that the American and Brazilian genotypes of E. canis are distributed evenly in Brazil, especially in the tropical region, while the temperate region in the south presented the lowest prevalence rates. This study offers the first report of dogs seropositive for the Costa Rican genotype in Brazil.

Column enzyme immunoassay for secretory immunoglobulin A in serum.

1983 ◽  
Vol 29 (1) ◽  
pp. 151-153 ◽  
Author(s):  
R Yamamoto ◽  
S Kimura ◽  
S Hattori ◽  
Y Ishiguro ◽  
K Kato
Keyword(s):  
Enzyme Immunoassay ◽  
Sodium Carbonate Solution ◽  
Solid Phase ◽  
Immunoglobulin A ◽  
Enzyme Reaction ◽  
Secretory Component ◽  
Secretory Immunoglobulin A ◽  
Serum Samples ◽  
Sepharose 4B ◽  
Secretory Immunoglobulin

Abstract This enzyme immunoassay for specific measurement of secretory immunoglobulin A concentrations in human serum involves use of a small chromatographic column as a solid-phase. Serum samples are incubated for 2 h with beta-D-galactosidase-labeled antibody to secretory component, then passed through a 0.1-mL Sepharose 4B column containing antibodies to human immunoglobulin A. After the column is washed to remove the unbound label, the buffer in the column is replaced by a solution of o-nitrophenyl-beta-D-galactoside (a beta-D-galactosidase substrate) and incubated at 25 degrees C overnight. The enzyme reaction is stopped by washing the column with sodium carbonate solution, and the absorbance of the eluate is measured at 420 nm. The concentration of secretory immunoglobulin A can be determined with a minimum detectable sensitivity of 3 mg/L, without interference from free immunoglobulin A and secretory component in the same samples.

scholarly journals P0364A NOVEL INTERPLAY BETWEEN INTESTINAL BACTERIA AND METABOLITES IN IGA NEPHROPATHY IDENTIFIED VIA INTEGRATED MICROBIOME AND METABOLOME APPROACHES

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Hongwei Wu ◽  
Yong Dai ◽  
Fanna Liu ◽  
Lianghong Yin
Keyword(s):  
Gut Microbiota ◽  
Immunoglobulin A ◽  
Intestinal Bacteria ◽  
Eicosatrienoic Acid ◽  
Glomerular Sclerosis ◽  
Serum Samples ◽  
Primary Glomerulonephritis ◽  
Liquid Chromatography Tandem Mass ◽  
Segmental Glomerular Sclerosis

Abstract Background and Aims Immunoglobulin A nephropathy (IgAN) is the most common form of primary glomerulonephritis. Intestinal bacteria and their metabolites have been implicated in various diseases. Improved understanding of the gut microbiota and its metabolic capabilities will facilitate development of diagnostic, therapeutic, and prognostic methods for IgAN Method We identified gut microbiota and metabolite biomarkers of IgAN by analyzing microbiomes and metabolomes of fecal and serum samples of IgAN patients and healthy controls using 16s ribosomal RNA gene sequencing and liquid chromatography-tandem mass spectrometry, respectively, and bioinformatics approaches Results We found that relative abundances of Streptococcus and Enterococcus were higher in IgAN patients, whereas Bacteroidetes and Bacteroides were lower. The changes in gut microbiota affected metabolism and absorbance of microbiota-associated metabolites of IgAN patients, in particular polyunsaturated fatty acids, free amino acids and oligopeptides, and activated the phenylalanine metabolism pathway. Also, 5-hydroxyeicosatetraenoic acid and 5-hydroxy-6E,8Z,11Z-eicosatrienoic acid were proved to be associated with the classification of segmental glomerular sclerosis but not 24h urine protein and estimated glomerular filtration rate. Conclusion Our findings demonstrate an interplay between intestinal bacteria and metabolites in IgAN. The identified metabolites may have diagnostic and therapeutic applications.

scholarly journals New Automated Immunoassay Measuring Immunoglobulin A Antigliadin Antibodies for Prediction of Celiac Disease in Childhood

2001 ◽  
Vol 8 (3) ◽  
pp. 564-570 ◽  
Author(s):  
E. Grodzinsky ◽  
A. Ivarsson ◽  
P. Juto ◽  
P. Olcén ◽  
K. Fälth-Magnusson ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Characteristic Curve ◽  
Immunoglobulin A ◽  
Total Serum ◽  
Small Intestinal Mucosa ◽  
Serum Samples ◽  
Serological Tests ◽  
Population Prevalence ◽  
Serum Iga ◽  
Antigliadin Antibodies

ABSTRACT The prevalence of celiac disease (CD) in Sweden is about 4 cases per 1,000 people. Screening for CD with serological tests indicates similar high prevalences in many other countries. Between 1 November 1992 and 30 April 1995, 133 children (9 months to 16.7 years of age) with suspected CD were studied. The predictive value (PV) of immunoglobulin A antigliadin antibodies (IgA-AGA) in the serum as assayed with two new commercial automated immunoassays—the Pharmacia CAP System Gliadin IgA FEIA (CAP) and the UNICAP-100 (UNICAP)—and with three “in-house” methods was evaluated using assessment of the small intestinal mucosa morphology as the “gold standard.” All serum samples were analyzed for total serum IgA. At presentation the diagnostic sensitivities and specificities of the different tests varied from 0.72 to 0.88 and 0.67 to 0.87, respectively. All methods showed a higher sensitivity for CD in younger children. The area under each assay's receiver operating characteristic curve was calculated and varied between 0.82 and 0.89. The positive and negative PVs for the CAP and UNICAP, which were assays with a high sensitivity and a high specificity, respectively, were estimated. In the clinically selected population (prevalence of CD, 1 in 3) the positive PV was about 55%, and in the general population (prevalence, 1 in 250) it was about 1%. The negative PVs for both CAP and UNICAP were close to 100%; thus, when the AGA test was negative, the risk for CD was small. Interestingly, five children had serum IgA levels below the detection limit (<0.07 g/liter) when on a gluten-free diet, whereas they had normal levels at the time of the first biopsy. In conclusion, the automated immunoassays—based on ImmunoCAP technology—for analysis of IgA-AGA had a reliability comparable to that of the in-house methods.

scholarly journals Leukogram Profile and Clinical Status invivaxandfalciparumMalaria Patients from Colombia

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Alberto Tobón-Castaño ◽  
Esteban Mesa-Echeverry ◽  
Andrés Felipe Miranda-Arboleda
Keyword(s):  
Hematological Parameters ◽  
Clinical Status ◽  
Clinical Complications ◽  
Cell Counts ◽  
Leukocyte Counts ◽  
White Blood Cell Counts ◽  
Hematologic Profile ◽  
Monitoring Treatment ◽  
The Relationship ◽  
Hematological Alterations

Introduction. Hematological alterations are frequent in malaria patients; the relationship between alterations in white blood cell counts and clinical status in malaria is not well understood. In Colombia, with low endemicity and unstable transmission for malaria, with malariavivaxpredominance, the hematologic profile in malaria patients is not well characterized. The aim of this study was to characterize the leukogram in malaria patients and to analyze its alterations in relation to the clinical status.Methods. 888 leukogram profiles of malaria patients from different Colombian regions were studied: 556 withP. falciparuminfection (62.6%), 313 withP. vivaxinfection (35.2%), and 19 with mixed infection by these species (2.1%).Results. Leukocyte counts at diagnosis were within normal range in 79% of patients and 18% had leucopenia; the most frequent alteration was lymphopenia (54%) followed by monocytosis (11%); the differential granulocyte count in 298 patients revealed eosinophilia (15%) and high basophil counts (8%). Leukocytosis, eosinopenia, and neutrophilia were associated with clinical complications. The utility of changes in leukocyte counts as markers of severity should be explored in depth. A better understanding of these hematological parameters will allow their use in prompt diagnosis of malaria complications and monitoring treatment response.

scholarly journals Effects of neem, betel leaf, devil's tree, jute and turmeric against gastrointestinal nematodes in sheep

1970 ◽  
Vol 8 (2) ◽  
pp. 259-263 ◽  
Author(s):  
MR Amin ◽  
M Mostofa ◽  
MN Islam ◽  
MA Asgar
Keyword(s):  
Body Weight ◽  
Hematological Parameters ◽  
Water Extract ◽  
Gastrointestinal Nematode ◽  
Experimental Period ◽  
Gastrointestinal Nematodes ◽  
The Body ◽  
Control Group ◽  
Betel Leaf ◽  
Group A

Effects of neem, betel leaf, devil’s tree, jute and turmeric against natural gastrointestinal nematodes in sheep and on some hematological parameters (TEC, Hb and PCV) and body weight were studied. Thirty (30) naturally parasitized sheep were randomly divided into six groups(A, B, C, D, E and F), each consisting of five sheep. Ten percent water extract of leaves of neem, betel leaf, devil’s tree and jute were administered orally to the sheep of group A, B, C and D, respectively. Sheep of group E was treated orally with 10% water extract of rhizome of turmeric. Sheep of group F was kept as infected control group. Fecal samples, hematological parameters and body weight were examined before treatment and on 7th, 14th, 21st and 28th day. A significant (p<0.01) reduction of EPG count was found following administration of neem (37.60-47.03%), betel leaf (6.43-14.00%), devil’s tree (3.04-11.04%), jute (0.50-5.26%) and turmeric (0.46-8.30) in sheep. The EPG count of the control group (F) were significantly (p<0.01) increased up to the last day of experimental period. After treatment with neem, betel leaf, devil’s tree, jute and turmeric total erythrocyte count (TEC), hemoglobin (Hb) content and packed cell volume (PCV) were increased significantly (p<0.01 and p<0.05) in sheep. The body weight was increased significantly (p<0.01 and p<0.05) in neem, betel leaf, devil’s tree, jute and turmeric treated sheep. On the other hand, body weight was decreased in untreated control group. The present study reveal that 10% water extract of neem was moderately effective and betel leaf, devil’s tree, jute and turmeric were relatively less effective against gastrointestinal nematodes in sheep. Keywords: Neem; Betel leaf; Devil’s tree; Jute; Turmeric; Gastrointestinal nematode DOI: 10.3329/jbau.v8i2.7935 J. Bangladesh Agril. Univ. 8(2): 259-263, 2010  

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