Clinical chemistry reference database for Wistar rats and C57/BL6 mice

2007 ◽  
Vol 388 (5) ◽  
pp. 547-554 ◽  
Author(s):  
Olaf Boehm ◽  
Berndt Zur ◽  
Alexander Koch ◽  
Nguyen Tran ◽  
Rainer Freyenhagen ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Clinical Chemistry ◽  
Reference Intervals ◽  
Reference Database ◽  
Sodium Potassium ◽  
Female Wistar Rats ◽  
Gender Specific Differences ◽  
Gender Specific ◽  
Retention Parameters ◽  
Important Marker

AbstractClinical chemistry data are decisive for evaluating altered organ function or damage in experimental animals. Few publications provide reliable clinical chemistry reference intervals, and analytical methods are often not described. Here, we investigated common clinical chemistry values in adult male and female Wistar rats and C57/BL6 mice (n=30/group). Blood samples were taken and analysed for electrolytes, substrates, metabolites and enzymes. In addition, we investigated cystatin C, an important marker of glomerular dysfunction. All data were obtained using commercially available kits frequently employed in most clinical chemistry laboratories and compared with data from other studies, as well as with human data. Significant gender-specific differences were observed in rats (electrolytes, retention parameters and transaminases) and in mice (cholesterol, glucose). High variability was noted for sodium, potassium, glucose, creatine kinase, lactate dehydrogenase and transaminase levels. Both rodent species showed markedly higher α-amylase activity than humans. This report demonstrates significant differences between genders for many analytes in rats and for fewer parameters in mice. Some reference values displayed major discrepancies between rodents and humans.

Population-based pediatric reference intervals for general clinical chemistry analytes on the Abbott Architect ci8200 instrument

2012 ◽  
Vol 50 (5) ◽  
Author(s):  
Peter Ridefelt ◽  
Mattias Aldrimer ◽  
Per-Olof Rödöö ◽  
Frank Niklasson ◽  
Leif Jansson ◽  
...  
Keyword(s):  
Clinical Chemistry ◽  
Population Based ◽  
Reference Intervals ◽  
Healthy Children ◽  
Age And Gender ◽  
Sodium Potassium ◽  
Daycare Centers ◽  
And Gender ◽  
Gender Specific ◽  
Abbott Architect

AbstractReference intervals are crucial decision-making tools aiding clinicians in differentiating between healthy and diseased populations. However, for children such values often are lacking or incomplete.Blood samples were obtained from 692 healthy children, aged 6 months to 18 years, recruited in daycare centers and schools. Twelve common general clinical chemistry analytes were measured on the Abbott Architect ci8200 platform; sodium, potassium, chloride, calcium, albumin-adjusted calcium, phosphate, magnesium, creatinine (Jaffe and enzymatic), cystatin C, urea and uric acid.Age- and gender specific pediatric reference intervals were defined by calculating the 2.5th and 97.5th percentiles.The data generated is primarily applicable to a Caucasian population when using the Abbott Architect platform, but could be used by any laboratory if validated for the local patient population.

Production of gender-specific morning salivary cortisol reference intervals using internationally accepted procedures

2004 ◽  
Vol 42 (12) ◽  
Author(s):  
Rajan S. Patel ◽  
Steve R. Shaw ◽  
Halena MacIntyre ◽  
Gerald W. McGarry ◽  
A. Michael Wallace
Keyword(s):  
Salivary Cortisol ◽  
Reference Data ◽  
Clinical Chemistry ◽  
Reference Intervals ◽  
International Federation ◽  
Adrenal Function ◽  
Biologically Active ◽  
Males And Females ◽  
Gender Specific ◽  
Free Plasma

AbstractBackground: Salivary cortisol concentrations correlate well with biologically active unbound free plasma cortisol concentrations. Despite its practical and analytical advantages, salivary cortisol measurement has been used mainly as a research tool rather than for the routine evaluation of adrenal function. This may be partly explained by the lack of robust reference data in the literature.Methods: Using the recommended procedures for the production of reference intervals published by the International Federation of Clinical Chemistry, we aimed to produce morning salivary cortisol reference intervals for males and females. Salivary cortisol was measured in 496 specimens collected from 248 reference individuals (128 males, median age 41years, range 16–86; and 120 females, median age 44years, range 16–98) attending an otorhinolaryngology clinic. Reference individuals mailed saliva specimens sampled on two consecutive mornings to our laboratory, where cortisol concentrations were measured.Results: Statistical analysis showed no significant correlation with age or body mass index. The following 95% gender-partitioned reference intervals were produced: males 10.9–40.3nmol/l; and females 9.3–40.3nmol/l.Conclusion: Knowledge of these salivary cortisol reference intervals helps us monitor the adrenal function of outpatients using topical intranasal glucocorticoids for rhinosinusitis.

scholarly journals Reference intervals for 24 laboratory parameters determined in 24-hour urine collections

2016 ◽  
Vol 54 (1) ◽  
Author(s):  
Raffaele Curcio ◽  
Helen Stettler ◽  
Paolo M. Suter ◽  
Jasmin Barman Aksözen ◽  
Lanja Saleh ◽  
...  
Keyword(s):  
Clinical Chemistry ◽  
Large Population ◽  
Population Based ◽  
Reference Intervals ◽  
Specific Reference ◽  
Urine Excretion ◽  
Sodium Potassium ◽  
Men And Women ◽  
Laboratory Parameters ◽  
Urine Cortisol

AbstractReference intervals for many laboratory parameters determined in 24-h urine collections are either not publicly available or based on small numbers, not sex specific or not from a representative sample.Osmolality and concentrations or enzymatic activities of sodium, potassium, chloride, glucose, creatinine, citrate, cortisol, pancreatic α-amylase, total protein, albumin, transferrin, immunoglobulin G, αBy contrast to most published reference intervals, which do not stratify for sex, reference intervals of 12 of 24 laboratory parameters in 24-h urine collections and of eight of 16 parameters as creatinine-normalized ratios differed significantly between men and women. For six parameters calculated as 24-h urine excretion and four parameters calculated as creatinine-normalized ratios no reference intervals had been published before. For some parameters we found significant and relevant deviations from previously reported reference intervals, most notably for 24-h urine cortisol in women. Ten 24-h urine parameters showed weak or moderate sex-specific correlations with age.By applying up-to-date analytical methods and clinical chemistry analyzers to 24-h urine collections from a large population-based cohort we provide as yet the most comprehensive set of sex-specific reference intervals calculated according to CLSI guidelines for parameters determined in 24-h urine collections.

Clinical chemistry reference database for Wistar rats and C57/BL6 mice

2007 ◽  
Vol 388 (11) ◽  
Author(s):  
Olaf Boehm ◽  
Berndt Zur ◽  
Alexander Koch ◽  
Nguyen Tran ◽  
Rainer Freyenhagen ◽  
...  
Keyword(s):  
Wistar Rats ◽  
Clinical Chemistry ◽  
Reference Database

scholarly journals Reference intervals for platelet indices in seniors and frequency of abnormal results in a population-based setting: a comparison between directly and indirectly estimated reference intervals

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Wolfgang Hermann ◽  
Lorenz Risch ◽  
Chris Grebhardt ◽  
Urs E. Nydegger ◽  
Benjamin Sakem ◽  
...  
Keyword(s):  
Population Based ◽  
Reference Intervals ◽  
Platelet Distribution Width ◽  
Platelet Volume ◽  
Age And Gender ◽  
Distribution Width ◽  
Platelet Indices ◽  
Gender Specific Differences ◽  
And Gender ◽  
Gender Specific

AbstractObjectivesMean platelet volume (MPV), platelet distribution width (PDW), and plateletcrit (PCT) possess diagnostic and prognostic capabilities in a variety of diseases. We aimed to establish reference intervals (RI) for platelet indices (PI) in seniors.MethodsWe established direct and indirect RI for MPV, PDW, and PCT in selected reference individuals aged 60 years and older. Abnormal PI were assessed in a population-based setting in the Principality of Liechtenstein, where 37.7% of the whole nation’s population aged 60 years and older had PI determined by hematology analyzers from Sysmex (Horgen, Switzerland).ResultsAmong 689 female and 542 male participants, MPV and PDW did not exhibit age- and gender-specific differences, whereas PCT in females also displayed no age-specific differences. Age- and sex-independent RI were 9.3–12.5 fl for MPV and 10.1–16.7% for PDW, whereas the age-independent RI for PCT in women was 0.18–0.37. In males, age-specific RI for PCT were 0.16–0.30 (age 60–69), 0.15–0.33 (age 70–79), and 0.14–0.33 (age 80 and older). The population-based frequency of abnormal PI results was 0.8% (MPV), 1.1% (PDW), and 24.4% (PCT).ConclusionsApplying novel RI for PI reveals that only approximately 1% of patients exhibit abnormal MPV and PDW. Abnormal PCT is observed much more frequently.

The effect of diet on the incidence of pituitary tumours in female Wistar rats

1984 ◽  
Vol 18 (3) ◽  
pp. 298-314 ◽  
Author(s):  
R. G. Pickering ◽  
C. Elizabeth Pickering
Keyword(s):  
Wistar Rats ◽  
Clinical Chemistry ◽  
The Other ◽  
Female Rats ◽  
Standard Diet ◽  
Pituitary Tumours ◽  
Synthetic Diet ◽  
Female Wistar Rats ◽  
Ad Libitum ◽  
Better Than

Each of 5 groups of 50 weanling Wistar-derived female rats was fed a different diet ad libitum for 2 years. The diets included 3 commercial maintenance diets (R and M No. 1, R and M No. 491 and RDM 22), a purified or semi-synthetic diet (LJ 80) and the diet (LAD 2) normally used in this laboratory. A weighed amount of LAD 2 diet (less than would be consumed under ad libitum conditions) was fed each day to a 6th group of rats. The survivals of the groups of rats fed either the standard diet (LAD 2) in restricted amount or the maintenance diet (RDM 22) were 62% and 66% respectively and were significantly better than the 40% survival of the group fed LAD 2 ad libitum. The survivals of the groups fed either Rand M No. 491 or LJ 80 were 40-42%, and the group fed Rand M No. 1 had a survival of 52%. At the end of 2 years the 2 groups with the best (>60%) survivals weighed significantly less than the group fed LAD 2 ad libitum. The groups fed either LAD 2 in restricted amount or RDM 22 had the least gross pathological lesions for the majority of the organs. These same groups also had fewer pituitary tumours per group (72% and 66%) than the group fed LAD 2 ad libitum (90%). The group fed RDM 22 also had the largest % (26%) of normal pituitaries of all the groups in the study. There were some significant differences between the haematological and clinical chemistry results of the group fed LAD 2 ad libitum and the other groups but none of the changes was considered adverse, although the groups fed either RDM 22 or Rand M No. 491 had significantly increased mean aminotransferase (AST and ALT) values with large variations which were adjudged undesirable. There was less protein in the urine of the groups fed either RDM 22 or LAD 2 (restricted).

scholarly journals Absence of Renal Protection of an Aqueous Extract of Lemongrass (Cymbopogon citratus) Cultivated in Costa Rica, Using a Model of Acute Renal Failure in Wistar Rats

2019 ◽  
Vol 7 (6) ◽  
pp. 11-19
Author(s):  
Apú Leitón Navilla ◽  
Fallas Ramírez Jose Manuel ◽  
Orozco Aguilar Josué ◽  
Rodríguez Arrieta Jesús Alexander ◽  
Mora Román Juan José
Keyword(s):  
Costa Rica ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Histopathological Analysis ◽  
Sodium Potassium ◽  
Female Wistar Rats ◽  
Nephroprotective Activity ◽  
Layer Chromatography

citratus, known as zacate de limón (lemongrass), is commonly used in Costa Rica for the treatment of "kidney diseases." Therefore, the activity as renal protector of an aqueous extract of this plant was evaluated after oral administration, in a model of acute kidney injury (AKI) in female Wistar rats. For this, an aqueous extract was characterized by thin layer chromatography and qualitative phytochemical tests. An AKI model induced by potassium chromate in female Wistar rats was carried out. The serum concentration of creatinine, sodium, potassium and glucose were determined, as well as the concentrations of creatinine, sodium, potassium, glucose and proteins in urine, together with the urinary flow determination. A histopathological analysis of the subjects’ kidneys was performed, and the presence of kidney damage was identified. The characterization of the extract by thin layer chromatography, and ferric chloride, Shinoda and Wilson tests gave negative results for phenols, tannins and flavonoids. No positive changes were observed in renal function markers in urine. No histopathological evidence of nephroprotective activity of the aqueous extract of C. citratus was found. It is concluded that the aqueous extract of C. citratus obtained by infusion does not possess nephroprotective activity at a dose of 150 mg/kg evaluated by the AKI in female Wistar rats.

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