Reference intervals for 24 laboratory parameters determined in 24-hour urine collections

AbstractReference intervals for many laboratory parameters determined in 24-h urine collections are either not publicly available or based on small numbers, not sex specific or not from a representative sample.Osmolality and concentrations or enzymatic activities of sodium, potassium, chloride, glucose, creatinine, citrate, cortisol, pancreatic α-amylase, total protein, albumin, transferrin, immunoglobulin G, αBy contrast to most published reference intervals, which do not stratify for sex, reference intervals of 12 of 24 laboratory parameters in 24-h urine collections and of eight of 16 parameters as creatinine-normalized ratios differed significantly between men and women. For six parameters calculated as 24-h urine excretion and four parameters calculated as creatinine-normalized ratios no reference intervals had been published before. For some parameters we found significant and relevant deviations from previously reported reference intervals, most notably for 24-h urine cortisol in women. Ten 24-h urine parameters showed weak or moderate sex-specific correlations with age.By applying up-to-date analytical methods and clinical chemistry analyzers to 24-h urine collections from a large population-based cohort we provide as yet the most comprehensive set of sex-specific reference intervals calculated according to CLSI guidelines for parameters determined in 24-h urine collections.

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Population-based pediatric reference intervals for general clinical chemistry analytes on the Abbott Architect ci8200 instrument

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2011-0787 ◽

2012 ◽

Vol 50 (5) ◽

Cited By ~ 17

Author(s):

Peter Ridefelt ◽

Mattias Aldrimer ◽

Per-Olof Rödöö ◽

Frank Niklasson ◽

Leif Jansson ◽

...

Keyword(s):

Clinical Chemistry ◽

Population Based ◽

Reference Intervals ◽

Healthy Children ◽

Age And Gender ◽

Sodium Potassium ◽

Daycare Centers ◽

And Gender ◽

Gender Specific ◽

Abbott Architect

AbstractReference intervals are crucial decision-making tools aiding clinicians in differentiating between healthy and diseased populations. However, for children such values often are lacking or incomplete.Blood samples were obtained from 692 healthy children, aged 6 months to 18 years, recruited in daycare centers and schools. Twelve common general clinical chemistry analytes were measured on the Abbott Architect ci8200 platform; sodium, potassium, chloride, calcium, albumin-adjusted calcium, phosphate, magnesium, creatinine (Jaffe and enzymatic), cystatin C, urea and uric acid.Age- and gender specific pediatric reference intervals were defined by calculating the 2.5th and 97.5th percentiles.The data generated is primarily applicable to a Caucasian population when using the Abbott Architect platform, but could be used by any laboratory if validated for the local patient population.

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Annual biological variation and personalized reference intervals of clinical chemistry and hematology analytes

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2021-0479 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Shuo Wang ◽

Min Zhao ◽

Zihan Su ◽

Runqing Mu

Keyword(s):

Clinical Chemistry ◽

Population Based ◽

Reference Intervals ◽

Biological Variation ◽

Annual Data ◽

Healthy Individuals ◽

Reference Change Value ◽

Index Of Individuality ◽

Personalized Diagnosis

Abstract Objectives A large number of people undergo annual health checkup but accurate laboratory criterion for evaluating their health status is limited. The present study determined annual biological variation (BV) and derived parameters of common laboratory analytes in order to accurately evaluate the test results of the annual healthcare population. Methods A total of 43 healthy individuals who had regular healthcare once a year for six consecutive years, were enrolled using physical, electrocardiogram, ultrasonography and laboratory. The annual BV data and derived parameters, such as reference change value (RCV) and index of individuality (II) were calculated and compared with weekly data. We used annual BV and homeostatic set point to calculate personalized reference intervals (RIper) which were compared with population-based reference intervals (RIpop). Results We have established the annual within-subject BV (CVI), RCV, II, RIper of 24 commonly used clinical chemistry and hematology analytes for healthy individuals. Among the 18 comparable measurands, CVI estimates of annual data for 11 measurands were significantly higher than the weekly data. Approximately 50% measurands of II were <0.6, the utility of their RIpop were limited. The distribution range of RIper for most measurands only copied small part of RIpop with reference range index for 8 measurands <0.5. Conclusions Compared with weekly BV, for annual healthcare individuals, annual BV and related parameters can provide more accurate evaluation of laboratory results. RIper based on long-term BV data is very valuable for “personalized” diagnosis on annual health assessments.

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Body Dissatisfaction and Disordered Eating in Androphilic and Gynephilic Men and Women

10.31234/osf.io/84kaj ◽

2018 ◽

Author(s):

Jan Antfolk ◽

Monica Ålgars ◽

Linn Holmgård ◽

Pekka Santtila

Keyword(s):

Body Mass Index ◽

Body Dissatisfaction ◽

Disordered Eating ◽

Minority Stress ◽

Large Population ◽

Population Based ◽

Objectification Theory ◽

Sexual Competition ◽

Men And Women ◽

Stress Theory

According to the theory of intra-sexual competition for mates, androphilic individuals (i.e., sexually interested in men) are expected to emphasize their physical appearance, and be less satisfied with their bodies, compared to gynephilic individuals (i.e., sexually interested in women). To test this theory against objectification theory and minority stress theory of body dissatisfaction and disordered eating, we measured body dissatisfaction, disordered eating, and body mass index (BMI) in both androphilic and gynephilic men and women in a large population-based sample (N = 8,007). Compared to gynephilic men, androphilic men were more likely to be dissatisfied with their bodies and display disordered eating. Gynephilic women were more dissatisfied with their bodies compared to androphilic women. Women were, on average, also more likely to be dissatisfied with their bodies and display disordered eating compared to men. Theoretical implications of the findings were discussed.

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Distribution of HOMA-IR in a population-based cohort and proposal for reference intervals

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2021-0643 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Bassel Matli ◽

Andreas Schulz ◽

Thomas Koeck ◽

Tanja Falter ◽

Johannes Lotz ◽

...

Keyword(s):

Insulin Resistance ◽

Fasting Glucose ◽

Reference Group ◽

Reference Interval ◽

Population Based ◽

Reference Intervals ◽

Health Study ◽

Specific Reference ◽

Model Assessment ◽

Significant Difference

Abstract Objectives Insulin resistance (IR) is a hallmark of type 2 diabetes mellitus (DM). The homeostatic model assessment of insulin resistance (HOMA-IR) provides an estimate for IR from fasting glucose and insulin serum concentrations. The aim of this study was to obtain a reference interval for HOMA-IR for a specific insulin immunoassay. Methods The Gutenberg Health Study (GHS) is a population-based, prospective, single-center cohort study in Germany with 15,030 participants aged 35–74 years. Fasting glucose, insulin, and C-peptide were available in 10,340 participants. HOMA-IR was calculated in this group and three reference subgroups with increasingly more stringent inclusion criteria. Age- and sex-dependent distributions of HOMA-IR and reference intervals were obtained. In a substudy three insulin assays were compared and HOMA-IR estimated for each assay. Results Among the 10,340 participants analyzed there were 6,590 non-diabetic, 2,901 prediabetic, and 849 diabetic individuals. Median (interquartile range [IQR]) HOMA-IR was 1.54 (1.13/2.19), 2.00 (1.39/2.99), and 4.00 (2.52/6.51), respectively. The most stringently selected reference group consisted of 1,065 persons. Median (IQR) HOMA-IR was 1.09 (0.85/1.42) with no significant difference between men and women. The 97.5th percentile was 2.35. There was a non-significant trend towards higher values with older age. Comparison of three immunoassays for insulin showed an unsatisfactory correlation among the assays and systematic differences in calculated HOMA-IR. Conclusions We present HOMA-IR reference intervals for adults derived by more or less stringent selection criteria for the reference cohort. In addition we show that assay specific reference intervals for HOMA-IR are required.

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Early pregnancy reference intervals; 29 serum analytes from 4 to 12 weeks’ gestation in naturally conceived and uncomplicated pregnancies resulting in live births

Clinical Chemistry and Laboratory Medicine (CCLM) ◽

10.1515/cclm-2019-0495 ◽

2019 ◽

Vol 57 (12) ◽

pp. 1956-1967 ◽

Cited By ~ 3

Author(s):

Jesper Friis Petersen ◽

Lennart J. Friis-Hansen ◽

Andreas Kryger Jensen ◽

Anders Nyboe Andersen ◽

Ellen C.L. Løkkegaard

Keyword(s):

Pregnant Women ◽

Early Pregnancy ◽

Clinical Chemistry ◽

Clinical Care ◽

Local Population ◽

First Trimester ◽

Reference Intervals ◽

Specific Reference ◽

Cancer Antigen 125 ◽

Serum Samples

Abstract Background Pregnancy introduces major physiological changes that also alter biochemical analytes. Maternal and perinatal health can be optimized by early intervention and therefore, pregnancy-specific reference intervals (RIs) for the local population are warranted. While the second and third trimester-specific changes are well described, the first trimester is less well characterized. We therefore wanted to facilitate early detection of abnormalities by generating first trimester reference values for 29 common analytes. Methods In a prospective early pregnancy (PEP) cohort (2016–2017), 203 pregnant women were recruited from 4 to 8 weeks’ gestation. Consecutive blood samples were drawn every 2 weeks until an ongoing second trimester pregnancy (n = 164) or a miscarriage (n = 39) occurred. After exclusion of women with complicated pregnancies or deliveries (n = 42), 122 women were included. The serum samples collected at <6, 6–8, 8–10, 10–12 and >12 weeks’ gestation were analyzed for 29 common analytes. Subsequently the RIs were calculated according to the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) recommendations (2.5–97.5th percentiles) and compared with the conventional RIs for non-pregnant women. Results Human chorionic gonadotropin (hCG), progesterone (P4), estradiol (E2), pregnancy-associated plasma protein A (PAPP-A), cancer antigen 125 (CA125), thyroid stimulating hormone (TSH), creatinine (CREA) and albumin (ALB) showed an early pregnancy-dependent change compared with conventional limits. For ALB the change was seen at 5.5 weeks’ gestation. Conclusions We report gestational age-specific RIs available from the early part of the first trimester applicable to everyday clinical care of pregnant women. Well-known alterations of RIs seen in later trimesters are also observed in the first.

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Age-specific reference intervals for anti-Müllerian hormone in Chinese boys: A population-based study

Clinica Chimica Acta ◽

10.1016/j.cca.2020.03.028 ◽

2020 ◽

Vol 506 ◽

pp. 154-159

Author(s):

Jin Wang ◽

Li Jiang ◽

Xinxin Zhang ◽

Shuo Gong ◽

Mengyi Chen ◽

...

Keyword(s):

Population Based ◽

Reference Intervals ◽

Specific Reference ◽

Population Based Study

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First Definition of Reference Intervals of Liver Function Tests in China: A Large-Population-Based Multi-Center Study about Healthy Adults

PLoS ONE ◽

10.1371/journal.pone.0072916 ◽

2013 ◽

Vol 8 (9) ◽

pp. e72916 ◽

Cited By ~ 19

Author(s):

Runqing Mu ◽

Wenxiang Chen ◽

Baishen Pan ◽

Lanlan Wang ◽

Xiaoke Hao ◽

...

Keyword(s):

Liver Function ◽

Large Population ◽

Liver Function Tests ◽

Population Based ◽

Reference Intervals ◽

Healthy Adults ◽

Function Tests ◽

Definition Of ◽

Multi Center Study ◽

Center Study

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Circulating levels of perfluoroalkyl substances and biomarkers of liver function in a large population based sample of elderly men and women from Sweden

Toxicology Letters ◽

10.1016/j.toxlet.2015.08.304 ◽

2015 ◽

Vol 238 (2) ◽

pp. S91

Author(s):

M. Lind ◽

S. Salihovic ◽

A. Larsson ◽

B. van Bavel ◽

L. Lind

Keyword(s):

Liver Function ◽

Large Population ◽

Population Based ◽

Perfluoroalkyl Substances ◽

Elderly Men ◽

Men And Women ◽

Circulating Levels

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Trimester-Specific Reference Ranges for Saturated, Monounsaturated and Polyunsaturated Fatty Acids in Serum of Pregnant Women: A Cohort Study from the ECLIPSES Group

Nutrients ◽

10.3390/nu13114037 ◽

2021 ◽

Vol 13 (11) ◽

pp. 4037

Author(s):

Carla Martín-Grau ◽

Ramón Deulofeu ◽

Nuria Serrat Orus ◽

Victoria Arija ◽

Keyword(s):

Fatty Acids ◽

Pregnant Women ◽

Large Population ◽

First Trimester ◽

Reference Intervals ◽

Gas Chromatography Mass Spectrometry ◽

Specific Reference ◽

Reference Ranges ◽

Maternal Factors ◽

Developed Country

In the course of pregnancy, increasing importance is being placed on maintaining optimal fatty acid (FA) levels and particularly n-3 PUFAs to ensure correct fetal development. However, reference ranges for FA have been reported in only a few studies. Our objective is to provide quantitative reference intervals for SFAs, MUFAs, and PUFAs (n-6 and n-3) in a large population of healthy pregnant women from a developed country. A prospective study of pregnant women (n = 479) was conducted from the first trimester (T1) to the third trimester (T3). A total of 11 fatty acids were analyzed in serum by gas chromatography mass spectrometry and were expressed as absolute (µmol/L) and relative (percentage of total FA) concentration units. Serum concentrations of SFAs, MUFAs, n-6 PUFAs, n-3 PUFAs, various FA ratios, and the EFA index were determined. The reference intervals (2.5/97.5 percentiles) in absolute values from T1 ranged from 1884.32 to 8802.81 µmol/L for SFAs, from 959.91 to 2979.46 µmol/L for MUFAs, from 2325.77 to 7735.74 µmol/L for n-6 PUFAs, and from 129.01 to 495.58 µmol/L for n-3 PUFAs. These intervals mainly include the values of other studies from European populations. However, reference ranges vary according to some maternal factors. The FA levels proposed, obtained from a large sample of pregnant women, will be a useful tool for assessing the degree of adequacy of FAs in pregnant women and will help to carry out dietary interventions based on certain maternal factors.

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