Antimicrobial Activity of Three Lamiaceae Essential Oils Against Common Oral Pathogens

Summary Chemical composition, antimicrobial and cytotoxic activities of commercial essential oils’ samples from the aerial plant parts of H. officinalis, R. officinalis and S. officinalis were investigated. Analyses by GC-FID and GC-MS confirmed 52 oil components. The major constituent of the H. officinalis oil was cis-pinocamphone (34.4%), followed by transpinocamphone (23.3%), and β-pinene (11.3%). Analysis of R. officinalis oil revealed 1.8-cineol as a major constituent (43.8%), as well as transpinocamphone (12.5%), α-pinene (11.5%) and β-pinene (8.2%). The most dominant constituent of S. officinalis oil was cis-thujone (32.7%), in addition to camphor (17.2%), 1.8-cineol (10.1%), α-pinene (8.6%), transthujone (7.7%) and camphene (7.3%). The essential oil antimicrobial activity assay was performed by the use of microdilution method against oral Candida spp. and bacteria, the major causative agents of a number of human oral disorders; all of them were susceptible to tested concentrations of H. officinalis, R. officinalis and S. officinalis essential oils, although the oil of S. officinalis exhibited the lowest antimicrobial potential. The results obtained in this study encourage use of investigated essential oils from Lamiaceae family in development of safe natural agents for prevention and/ or alternative therapy of human oral diseases. However, a special care during development of an effective natural preparation is required.

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Chemical Composition and Bactericidal Activity of the Essential Oils of Four Species of Annonaceae Growing in Brazilian Amazon

Natural Product Communications ◽

10.1177/1934578x1701200437 ◽

2017 ◽

Vol 12 (4) ◽

pp. 1934578X1701200 ◽

Cited By ~ 2

Author(s):

Joelma Moreira Alcântara ◽

Juliana Mesquita V. M. de Lucena ◽

Roselaine Facanali ◽

Marcia Ortiz M. Marques ◽

Maria da Paz Lima

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Brazilian Amazon ◽

Major Constituent ◽

Caryophyllene Oxide ◽

Strong Activity ◽

Gram Positive Bacteria ◽

Microdilution Method

A study was conducted to determine the chemical composition and the antibacterial activity of essential oils from Bocageopsis multiflora (Mart.) R.E. Fr., Ephedranthus amazonicus R.E. Fr., Guatteria blepharophylla Mart. and Xylopia aromatica (Lam.) Mart. The essential oils were obtained from the leaves by hydrodistillation and analyzed by GC-FID and GC-MS. The chromatograms showed the predominance of sesquiterpenoids. Spathulenol was the major constituent of oils of B. multiflora (20.3%), X. aromatica (21.5%) and E. amazonicus (16.9%); followed by caryophyllene oxide of G. blepharophylla (55.7%). Other constituents with significant percentages were the sesquiterpenes β-bisabolene (11.9%) in the samples oil of B. multiflora, humulene epoxide II (16.3%) of E. amazonicus, the monoterpenes trans-pinocarveol (10.2%) and dihydrocarveol (11.6%) of X. aromatica. The essential oils of X. aromatica and G. blepharophylla showed strong activity against Gram-positive bacteria Streptococcus sanguinis by microdilution method. The antimicrobial activity against Staphylococcus aureus also was detected by G. blepharophylla essential oil.

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Antimicrobial Essential Oil Combinations to Combat Foot Odour

Planta Medica ◽

10.1055/a-0592-8022 ◽

2018 ◽

Vol 84 (09/10) ◽

pp. 662-673 ◽

Cited By ~ 4

Author(s):

Ané Orchard ◽

Alvaro Viljoen ◽

Sandy van Vuuren

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Essential Oils ◽

Antimicrobial Efficacy ◽

Juniperus Virginiana ◽

Broth Microdilution ◽

Synergistic Interactions ◽

Microdilution Method ◽

Broth Microdilution Method

AbstractFoot odour (bromodosis) is an embarrassing and perplexing condition mostly caused by bacteria of the Brevibacterium species. Essential oils are a credible option as an affordable treatment of odour and contribute towards antimicrobial efficacy. Therefore, this study sets out to investigate the antimicrobial activity of essential oil combinations against odour-causing bacteria. The broth microdilution method was used to investigate the antimicrobial activity of 119 essential oil combinations, and the fractional inhibitory index was calculated to determine the interactive profile. Combinations that resulted in synergy in 1 : 1 ratios were further evaluated in different concentrations, and isobolograms were plotted to determine the influence of the ratio on overall activity. Numerous combinations could be identified as having synergistic interactions against the Brevibacterium spp. and no antagonism was observed. The combination of Juniperus virginiana (juniper) and Styrax benzoin (benzoin) demonstrated synergy against all three Brevibacterium spp. tested and J. virginiana was the essential oil responsible for the majority of the synergistic interactions. The results reported here confirm the promising potential of the majority of these oils and selected combinations in treating and controlling bromodosis.

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Chemical Composition, Antioxidant, Anti-Tyrosinase, Anti-Cholinesterase and Cytotoxic Activities of Essential Oils of Six Algerian Plants

Molecules ◽

10.3390/molecules25071710 ◽

2020 ◽

Vol 25 (7) ◽

pp. 1710 ◽

Cited By ~ 2

Author(s):

Kadour Cheraif ◽

Boulanouar Bakchiche ◽

Abdelaziz Gherib ◽

Sanaa K. Bardaweel ◽

Melek Çol Ayvaz ◽

...

Keyword(s):

Essential Oils ◽

Cytotoxic Effect ◽

Food Preservation ◽

Major Constituent ◽

Cytotoxic Activities ◽

Mentha Pulegium ◽

Antioxidant Power ◽

Ic50 Values ◽

Lavandula Officinalis ◽

Main Component

In this study, the essential oils (EOs) of six Algerian plants (Artemisia campestris L., Artemisia herba-alba Asso, Juniperus phoenicea L., Juniperus oxycedrus L., Mentha pulegium L. and Lavandula officinalis Chaix) were obtained by hydrodistillation, and their compositions determined by GC-MS and GC-FID. The antioxidant activity of the EOS was evaluated via 2,2′-diphenyl-1-picrylhydrazyl (DPPH), ferric-reducing/antioxidant power (FRAP) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) assays. Moreover, their cytotoxic effect was evaluated—as well as their tyrosinase, acetyl- and butyryl-cholinesterase (AChE and BuChE) inhibitory activities. The chemical analyses detected 44, 45, 51, 53, 26 and 40 compounds in EOs of A. campestris, A. herba-alba, J. phoenicea, J. oxycedrus, M. pulegium and L. officinalis, respectively. A. campestris EO was mainly composed of β-pinene (20.7%), while A. herba-alba EO contained davanone D (49.5%) as the main component. α-Pinene (41.8%) was detected as the major constituent in both J. phoenicea (41.8%) and J. oxycedrus (37.8%) EOs. M. pulegium EO was characterized by pulegone as the most abundant (76.9%) compound, while linalool (35.8%) was detected as a major constituent in L. officinalis EO. The antioxidant power evaluation revealed IC50 values ranging from 2.61 to 91.25 mg/mL for DPPH scavenging activity, while the FRAP values ranged from 0.97–8.17 µmol Trolox equivalents (TX)/g sample. In the ABTS assay, the values ranged from 7.01 to 2.40 µmol TX/g sample. In the presence of 1 mg/mL of the samples, tyrosinase inhibition rates ranged from 11.35% to 39.65%, AChE inhibition rates ranged from 40.57% to 73.60% and BuChE inhibition rates ranged from 6.47% to 72.03%. A significant cytotoxic effect was found for A. herba-alba EO. The obtained results support some of the traditional uses of these species in food preservation and for protection against several diseases.

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ANTIMICROBIAL AND SYNERGISTIC ACTIVITY OF ESSENTIAL OILS FACING ISOLATED BACTERIA FROM SURGICAL STAFF’S

Revista Prevenção de Infecção e Saúde ◽

10.26694/repis.v5i0.8970 ◽

2019 ◽

Vol 5 ◽

Author(s):

Tatiana Areas Cruz ◽

Fernanda Rodrigues Torres ◽

Monise Fazolin Petrucelli ◽

Mariana Heinzen De Abreu ◽

Silvia Sidneia Silva ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Staphylococcus Epidermidis ◽

Citrus Sinensis ◽

Concentration Index ◽

Inhibitory Concentration ◽

Thymus Vulgaris ◽

Synergistic Activity ◽

Bacterial Strains ◽

Microdilution Method

Objective:to evaluate antimicrobial and synergistic activity of essential oils (Citrus aurantifolia, Citrus sinensis, Mentha viridisand Thymus vulgaris) against isolated bacteria from surgical staff’s hands and bacterial strains. Method:the antimicrobial activity of oils was analyzed by microdilution method to determine the Minimum Inhibitory Concentration. Moreover, the combinatory effect of the oil that presented greater effectiveness using gentamycin sulphate through the Fractional Inhibitory Concentration index was tested. The study was performed over the period 2016-2018. Results:T. vulgaris was effective against Staphylococcus saprophyticus with a concentration of 0.0008 µL.mL-1and against the ATCC standard strains of Staphylococcus aureus, Salmonella choleraesuis,Staphylococcus epidermidis and Proteus vulgarisand the bacteria S. aureus, S. epidermidisand Bacillus sp. with MIC ≥ 0.10 µL.mL-1. This oil association with gentamycin sulphate showed synergistic activity againstS. epidermidis. Conclusion:T. vulgarisessential oil showed expressive antimicrobial activity against ATCC and isolated clinic microorganisms, suggesting broad spectrum of activity.

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Antimicrobial Activity of Selected Essential Oils against Bacteria Isolated from Freshwater Fish

Advanced Research in Life Sciences ◽

10.2478/arls-2021-0023 ◽

2021 ◽

Vol 5 (1) ◽

pp. 1-11

Author(s):

Miroslava Kačániová ◽

Alīna Klūga ◽

Margarita Terentjeva ◽

Simona Kunová ◽

Katarína Rovná ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Enterococcus Faecium ◽

Diffusion Method ◽

Disc Diffusion ◽

Yersinia Ruckeri ◽

Disc Diffusion Method ◽

Delftia Acidovorans ◽

Litsea Cubeba ◽

Microdilution Method

Abstract The aim of study was to determine the antimicrobial activity of ten essential oils against ten microorganisms isolated from fish. The following essential oils were tested: Cymbopogon nardus Rendle, Citrus limon, Leptospermum petersonii Bailey, Gaultheria procumbens, Pelargonium graveolens, Citrus paradisi, Citrus aurantifolia, Litsea cubeba Pers., Citrus reticulata Blanko, Vetiveria zizanoides. The disc diffusion method and microdilution method were used for determination of antimicrobial activity. Rhodotorula spp., Aerococcus spp., Aerococcus viridans, Enterococcus faecium, Enterococcus moraviensis, Aeromonas spp., Delftia acidovorans, Pseudomonas gessardii, Yersinia spp., Yersinia ruckeri, Yersinia enterocolitica were tested in the present study. The best antimicrobial activity of Leptospermum petersonii Bailey were found against Aerococcus spp., Enterococcus faecium, E. moraviensis, Delftia acidovorans, Pseudomonas gessardii, Yersinia ruckeri and Y. enterocolitica. Litsea cubeba Pers. was effective against Delftia acidovorans, Yersinia enterocolitica, Enterococcus faecium using the disc diffusion method. The best minimal inhibition concentration (MIC 50 - 1.5 µL/mL resp. MIC 90 - 1.6 µL/mL) was found for Litsea cubeba Pers. against Aerococcus spp. and Yersinia spp. using microdilution method.

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Chemical Composition and Bio-efficacy of Essential Oils from Italian Aromatic Plants: Mentha suaveolens, Coridothymus capitatus, Origanum hirtum and Rosmarinus officinalis

Natural Product Communications ◽

10.1177/1934578x1601101023 ◽

2016 ◽

Vol 11 (10) ◽

pp. 1934578X1601101 ◽

Cited By ~ 7

Author(s):

Antonella Spagnoletti ◽

Alessandra Guerrini ◽

Massimo Tacchini ◽

Vittorio Vinciguerra ◽

Claudia Leone ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Antioxidant Activities ◽

Rosmarinus Officinalis ◽

Cytotoxic Activities ◽

Hacat Cells ◽

Aromatic Plants ◽

Microdilution Method ◽

Human Keratinocyte ◽

Lung Cancer A549 Cell

The chemical composition, antifungal, antioxidant and cytotoxic activities of the essential oils (EOs) of mint (Mentha suaveolens), thyme (Coridothymus capitatus), oregano (Origanum hirtum) and rosemary (Rosmarinus officinalis) were investigated. The antifungal properties of the EOs were investigated against four species of Candida by a microdilution method. Cytotoxicity was tested on human keratinocyte (HaCaT) and lung cancer (A549) cell lines using the MTT test. DPPH• and ABTS•+ spectrophotometric assays and DPPH•- ABTS•+(HP)TLC-bioautographic assays were used to evaluate the antioxidant activity. The main compounds of thyme and oregano EOs were carvacrol and thymol, respectively; piperitenone oxide and γ-terpinene were the most abundant compounds of mint and rosemary EOs, respectively. All EOs showed activity against all Candida species in a range between 760 ± 290 to 3120 ± 0.0 μg/mL. Among the EOs, that of M. suaveolens showed a stronger cytotoxic activity on HaCaT cells. Thyme, oregano and rosemary EOs exhibited important antioxidant activities by ABTS•+ assay compared with trolox.

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Chemical composition and antimicrobial activity of Erodium species: E. ciconium L., E. cicutarium L., and E. absinthoides Willd. (Geraniaceae)

Chemical Papers ◽

10.2478/s11696-010-0014-x ◽

2010 ◽

Vol 64 (3) ◽

Cited By ~ 10

Author(s):

Zorica Stojanović-Radić ◽

Ljiljana Čomić ◽

Niko Radulović ◽

Milan Dekić ◽

Vladimir Ranđelović ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Palmitic Acid ◽

Gram Negative Bacteria ◽

Broth Microdilution ◽

Bacterial Strains ◽

Gram Negative ◽

Microdilution Method ◽

Broth Microdilution Method

AbstractThe present study gives results of chemical composition analyses and antimicrobial activity testing of three Erodium species: E. ciconium L., E. cicutarium L., and E. absinthoides Willd. Essential oils were obtained by hydro-distillation from air-dried entire plants and analyzed by GC and GC-MS. A total of 209 different compounds were identified: 162 for E. cicutarium, 107 for E. ciconium, and 79 for E. absinthoides. Antimicrobial activity (broth microdilution method) of the oils was screened against a panel of Gram positive and Gram negative bacteria and a number of fungi. Moderate susceptibility of all tested strains was observed. Determined MIC values were 0.156–5 mg mL−1 (bacterial strains) and 0.039–0.325 mg mL−1 (fungal strains). Major component of the most active oil, palmitic acid, was also tested for activity together with stearic and myristic acids.

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Potential Interaction between the Volatile and Non-volatile Fractions on the In Vitro Antimicrobial Activity of Three South African Pelargonium (Geraniaceae) Species

Natural Product Communications ◽

10.1177/1934578x1000500911 ◽

2010 ◽

Vol 5 (9) ◽

pp. 1934578X1000500 ◽

Cited By ~ 1

Author(s):

Jacqueline Y. Lalli ◽

Alvaro M. Viljoen ◽

Sandy F. Van Vuuren

Keyword(s):

Antimicrobial Activity ◽

Essential Oils ◽

Plant Material ◽

Potential Interaction ◽

Major Constituent ◽

Volatile Fraction ◽

Volatile Components ◽

Volatile Constituents ◽

Pharmacological Interaction

Previous studies have reported promising antimicrobial efficacy for the essential oils and solvent extracts of several indigenous Pelargonium species. This study aimed to determine if any pharmacological interaction (e.g. synergism or antagonism) exists between the volatile and non-volatile components when the different fractions were investigated. The antimicrobial activity of the following fractions were tested; the essential oil prepared by hydrodistillation (EO), non-volatile fraction (NV), prepared by extraction of plant material remaining in the distilling apparatus (having no or negligible volatile constituents) and solvent extracts prepared from fresh (FC) and dried (DC) plant material containing both volatile and non-volatile constituents. Pelargonium quercifolium oil was dominated by p-cymene (42.1%) and viridiflorol (16.9%), while P. graveolens and P. tomentosum oil had high levels of isomenthone (84.0 and 58.8%, respectively). Menthone was noted as a major constituent in the P. tomentosum EO sample. It was evident from the results that the presence of volatile constituents in the three species; P. graveolens, P. quercifolium and P. tomentosum is generally not a prerequisite for antimicrobial activity. The most significant variations of antimicrobial activity were noted for P. tomentosum where poorer activity was noted for the FC and EO fractions against Bacillus cereus and Candida albicans. Studies on Staphylococcus aureus, however, showed the converse, where best activity was noted for the FC fraction (3.0 mg/mL). For P. quercifolium, the DC fraction indicated a notable increase in anti-staphylococcal activity (2.0 mg/mL) when compared with the FC (8.0 mg/mL) and EO (16.0 mg/mL) fractions. For P. tomentosum, the FC fraction indicated much lower antimicrobial activity (against both B. cereus and C. albicans) when compared with all other fractions, suggesting that the essential oils may impact negatively on the antimicrobial activity when tested against these two pathogens.

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Chemical Composition and Antimicrobial Activity of Essential Oils from Centaurea Pannonica and C. Jacea

Natural Product Communications ◽

10.1177/1934578x1000501030 ◽

2010 ◽

Vol 5 (10) ◽

pp. 1934578X1000501 ◽

Cited By ~ 3

Author(s):

Tanja Milošević ◽

Catherine Argyropoulou ◽

Slavica Solujić ◽

Dragana Murat-Spahić ◽

Helen Skaltsa

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Fatty Acid Fraction ◽

Antimicrobial Activities ◽

Octadecanoic Acid ◽

Gram Positive ◽

Microdilution Method ◽

Important Fatty Acid ◽

Main Components

The chemical composition and antimicrobial activity of the essential oils obtained by hydrodistillation from Centaurea pannonica (Heufel) Simonkai and C. jacea L. (Asteraceae), were investigated. The essential oils were analyzed by GC and GC-MS. Forty five and twenty nine compounds were identified in the two oils, respectively. C. pannonica oil was rich in fatty acids (43.7%), with 9-octadecanoic acid (34.0%) and ( Z,Z)-9,12-octadecadienoic acid (8.6%) as the major compounds. In contrast, the essential oil of C. jacea was dominated by oxygenated sesquiterpenes (43.2%), among which caryophyllene oxide (23.5%) and spathulenol (8.9%) were the major constituents. However, the oil was also characterized by an important fatty acid fraction (15.5%), with 9-octadecanoic acid (8.9%) and hexadecanoic acid (6.6%) being the main components. The antimicrobial activities of the essential oils were evaluated by the microdilution method against three Gram-positive and three Gram-negative bacteria, and one yeast. Both oils exhibited significant antimicrobial activity, especially against Gram-positive bacteria.

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Chemical Composition and Antimicrobial Activity of Essential Oil from Phytolacca dodecandra Collected in Ethiopia

Molecules ◽

10.3390/molecules24020342 ◽

2019 ◽

Vol 24 (2) ◽

pp. 342 ◽

Cited By ~ 3

Author(s):

Wondwosen Matebie ◽

Wanchang Zhang ◽

Guangbo Xie

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Solid Phase ◽

Antimicrobial Activities ◽

Gas Chromatography Mass Spectrometry ◽

Microdilution Method ◽

Phytolacca Dodecandra ◽

The Difference

The essential oil from Phytolacca dodecandra, a traditional herb of Ethiopia, has been studied, including the chemical composition and antimicrobial activity. The difference between four P. dodecandra samples (P-1–P-4), which differed in gender or location, has also been analyzed. The essential oils were obtained by steam distillation, while the aromas were extracted by head space solid-phase microextraction (HS-SPME) and both were analyzed by gas chromatography- mass spectrometry (GC-MS). The oils’ antimicrobial activities were evaluated by the microdilution method against Escherichia coli, Staphylococcus aureus, Bacillus subtilis and Candida albicans. Ninety one components, representing 88.37 to 94.01% of the aromas, were identified. The compositions of the aromas of four samples are mainly dominated by aldehydes and ketones: 2-nonanone (1.80–30.80%), benzaldehyde (4.99–25.99%), and sulcatone (2.34–5.87%). Sixty components representing 64.61 to 69.64% of the oils were identified, and phytone (3.04–21.23%), phytol (4.11–26.29%) and palmitic acid (1.49–23.87%) are the major compounds. No obvious antimicrobial activity was observed for all the four essential oils.

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