Preparation of a novel superhydrophobic PMMA surface with nanostructure and its blood compatibility

e-Polymers ◽  
2012 ◽  
Vol 12 (1) ◽  
Author(s):  
Xiaobo Wang ◽  
Ningbo Yang ◽  
Qi Xu ◽  
Chun Mao ◽  
Xiaomei Hou ◽  
...  
Keyword(s):  
Blood Compatibility ◽  
Platelet Rich Plasma ◽  
Surface Group ◽  
Pmma Film ◽  
Selective Solvent ◽  
Water Contact ◽  
Human Whole Blood ◽  
Application Range ◽  
Pmma Surface ◽  
Better Than

AbstractSuperhydrophobic poly(methylmethacrylate) (PMMA) surface with nanostructure was prepared effectively via phase separation and selective solvent method. It is proposed that the cooperation of the nanostructure of PMMA surface and the surface group reorientation leads to the superhydrophobicity of PMMA surface. The superhydrophobic PMMA surface with nanostructure was characterized by scanning electron microscope (SEM) and water contact angle (WCA) measurements. The blood compatibility of the superhydrophobic PMMA surface with nanostructure was evaluated by platelet-rich plasma (PRP) and fresh human whole blood contacting experiments using pure flat PMMA film as the control. These two experiments demonstrated that blood compatibility of the superhydrophobic PMMA surface with nanostructure is better than that of the pure flat PMMA film. The significance of this research lies in that it will greatly broaden the application range of materials that can be used as biomaterials by fabrication of superhydrophobic surfaces.

Preparation of β-Cyclodextrin Modified Poly(d,l-Lactic Acid) and its Blood Compatibility

2011 ◽  
Vol 181-182 ◽  
pp. 429-433
Author(s):  
Jiu Jin Zhu ◽  
Xue Jun Quan ◽  
Yuan Liang Wang ◽  
Shu Shu Zou
Keyword(s):  
Contact Angle ◽  
Lactic Acid ◽  
Blood Compatibility ◽  
Blood Platelets ◽  
Acylating Agent ◽  
Acylation Reaction ◽  
Water Contact ◽  
Static Water Contact Angle ◽  
Static Water ◽  
Better Than

Aiming to develop a biomaterial with excellent biocompability, a novel β-cyclodextrin modified polylactic acid (PLA-β-CD) was synthesized by N-acylation reaction of MPLA (maleic anhydride modified poly(d,l-lactic acid)) using hydrophilic mono(6-(2-aminoethyl)-amino-6-deoxy)- β-cyclodextrin(β-CD-6-E) as acylating agent. Surface wettability and blood biocompatibility of PLA-β-CD were investigated. The tests indicated that the static water contact angle of PLA-β-CD was decreased from 76.7º to 72.1º comparing with PLA. The morphology of blood platelets adhered on the surface of PLA-β-CD was observed by scanning, and only a few platelets, round and not aggregated. The results revealed that the blood compatibility of PLA-β-CD was better than PLA.

Biodegradable Microfluidic Device: Hydrolysis, Decomposition and Surface Modification

2010 ◽  
Vol 447-448 ◽  
pp. 755-759 ◽  
Author(s):  
Jia En Low ◽  
Wei Xiang Koh ◽  
Joon Kit Lai ◽  
Yan Jie Lee ◽  
Xu Li ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Microfluidic Device ◽  
Ph Value ◽  
Blood Compatibility ◽  
Blood Clot ◽  
Hydrophobic Surface ◽  
Poly Lactic Acid ◽  
Water Contact ◽  
Chemical Grafting ◽  
Poly Ethylene

Poly(lactic acid) (PLA) is a biodegradable and biocompatible aliphatic polyester whose lactic acid monomers are derived from renewable resources such as corn and sugar beet. As a thermal plastic it can be processed through compounding and injection. As such, we have developed a microfludic device using PLA aimed at blood dialysis application. To quantify the degradation of PLA, its hydrolysis at different pH value was studied. To study the bioresorbable property of these fabricated devices, its decomposition was tested by morphology observation and weight change measurements after embedding in soil under simulated environmental conditions. Upon contact with a hydrophobic surface, platelets and prothrombin are always activated to attach to the surface, resulting in blood clot. This would block the blood flow through the dialysis channels in the microfluidic device. To improve the hydrophilicity, hence the blood compatibility, chemical grafting of a hydrophilic polymer, poly(ethylene oxide) methacrylate (PEGmA), onto the surface of PLA microfluidic device was carried out and the changes in hydrophilicity was monitored through measuring the water contact angle. Our results indicate that chemical grafting of PEGmA significantly improves the hydrophilicity of the device surface.

scholarly journals Adhesion and Proliferation of Osteoblast-Like Cells on Porous Polyetherimide Scaffolds

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Yanbo Zhang ◽  
Ruiyan Li ◽  
Wenzheng Wu ◽  
Yun’an Qing ◽  
Xiongfeng Tang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Contact Angle ◽  
Cell Adhesion ◽  
Phase Composition ◽  
Cell Morphology ◽  
Solvent Casting ◽  
Water Contact ◽  
Particulate Leaching ◽  
Cell Adhesion And Proliferation ◽  
Better Than

The purpose of this work was to investigate the porous polyetherimide scaffold (P-PEIs) as an alternative biopolymer for bone tissue engineering. The P-PEIs was fabricated via solvent casting and particulate leaching technique. The morphology, phase composition, roughness, hydrophilicity, and biocompatibility of P-PEIs were evaluated and compared with polyetherimide (PEI) and Ti6Al4V disks. P-PEIs showed a biomimetic porous structure with a modulus of 78.95 ± 2.30 MPa. The water contact angle of P-PEIs was 75.4 ± 3.39°, which suggested that P-PEIs had a wettability surface. Moreover, P-PEIs provides a feasible environment for cell adhesion and proliferation. The relative cell adhesion capability and the cell morphology on P-PEIs were better than PEI and Ti6Al4V samples. Furthermore, the MC3T3-E1 cells on P-PEIs showed faster proliferation rate than other groups. It was revealed that the P-PEIs could be a potential material for the application of bone regeneration.

scholarly journals Hydrophobic Starch-Based Films Using Potato Washing Slurries and Spent Frying Oil

Foods ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2897
Author(s):  
Sílvia Petronilho ◽  
André Oliveira ◽  
M. Rosário Domingues ◽  
Fernando M. Nunes ◽  
Manuel A. Coimbra ◽  
...  
Keyword(s):  
Potato Starch ◽  
Contact Angles ◽  
Single Step ◽  
Frying Oil ◽  
Biodegradable Films ◽  
Water Contact ◽  
Alkaline Catalyst ◽  
Application Range ◽  
Structural Differences

Starch is a promising candidate for preparing biodegradable films with useful gas barriers and thermoplastic capabilities. However, these materials are hydrophilic and brittle, thus limiting their application range. To overcome these drawbacks, it has been hypothesized that starch can be hydrophobized and plasticized during the starch-based film production using a single-step approach and following transesterification principles. In this work, KOH powder and spent frying oil (SFO) were used as an alkaline catalyst and a source for triacylglycerides, respectively, to promote the modification of starch. Different ratios of SFO (w/w related to the dried starch weight) were tested. When compared to the neat films (without a catalyst and SFO), the incorporation of at least 15% SFO/KOH gave rise to transparent, hydrophobic (water contact angles of ca. 90°), stretchable (ca. 20×), elastic (ca. 5×), and water tolerant starch-based films, contrary to the films produced without the catalyst. ATR-FTIR and 1H NMR revealed structural differences among the produced films, suggesting that starch was modified with the SFO-derived fatty acids. Therefore, adding KOH during the potato starch/spent frying oil-based film’s production was determined to be a promising in situ strategy to develop starch-based materials with improved hydrophobicity and flexibility, while valorizing the potato chip industry’s byproducts.

Endotoxin-Induced Platelet Activation in Human Whole Blood In Vitro

1988 ◽  
Vol 59 (03) ◽  
pp. 378-382 ◽  
Author(s):  
Gyorgy Csako ◽  
Eva A Suba ◽  
Ronald J Elin
Keyword(s):  
Platelet Aggregation ◽  
Whole Blood ◽  
Human Platelet ◽  
Platelet Rich Plasma ◽  
Atp Release ◽  
Human Platelets ◽  
Lag Phase ◽  
Human Whole Blood ◽  
Dose Dependent

SummaryThe effect of purified bacterial endotoxin was studied on human platelets in vitro. In adding up to 1 μg/mL of a highly purified endotoxin, we found neither aggregation nor ATP release in heparinized or citrated human platelet-rich plasma. On the other hand, endotoxin at concentrations as low as a few ng/mL (as may be found in septic patients) caused platelet aggregation in both heparinized and citrated human whole blood, as monitored by change in impedance, free platelet count, and size. Unlike collagen, the platelet aggregation with endotoxin occurred after a long lag phase, developed slowly, and was rarely coupled with measurable release of ATP. The platelet aggregating effect of endotoxin was dose-dependent and modified by exposure of the endotoxin to ionizing radiation. Thus, the activation of human platelets by “solubilized” endotoxin in plasma requires the presence of other blood cells. We propose that the platelet effect is mediated by monocytes and/or neutrophils stimulated by endotoxin.

In Vitro Biocompatibility of a New High Nitrogen Nickel Free Austenitic Stainless Steel

2007 ◽  
Vol 342-343 ◽  
pp. 605-608 ◽  
Author(s):  
Yi Bin Ren ◽  
Hua Juan Yang ◽  
Ke Yang ◽  
Bing Chun Zhang
Keyword(s):  
Stainless Steel ◽  
Austenitic Stainless Steel ◽  
Blood Compatibility ◽  
Platelet Rich Plasma ◽  
Clotting Time ◽  
Adhesion Test ◽  
High Nitrogen ◽  
In Vitro Biocompatibility ◽  
Time Test

The in vitro blood compatibility of a new nickel free high nitrogen austenitic stainless steel Fe-Cr-Mn-Mo-N (BIOSSN4) was studied by the kinetic clotting time test and the platelet rich plasma adhesion test in this paper. In comparison with 316L stainless steel, the kinetic clotting time of BIOSSN4 steel are longer, and only causes less activation of platelets in platelet adhesion test, which was indicated by their morphology and low spreading. The experimental results reveals that the BIOSSN4 stainless steel has better blood compatibility, the blood compatibility mechanism of steels was analyzed based on surface tension and interfacial tension between the steels and blood.

Blood Compatibility of Metal Oxide Layers on Stainless-steel

2002 ◽  
Vol 734 ◽  
Author(s):  
Kanji Tsuru ◽  
Shinji Takemoto ◽  
Tatsuhiro Yamamoto ◽  
Satoshi Hayakawa ◽  
Akiyoshi Osaka ◽  
...  
Keyword(s):  
Stainless Steel ◽  
Oxide Layer ◽  
Titanium Oxide ◽  
Platelet Adhesion ◽  
Blood Compatibility ◽  
Platelet Rich Plasma ◽  
Sol Gel ◽  
Dip Coating ◽  
Titanium Oxide Layer ◽  
Oxide Layers

ABSTRACTWe examined blood compatibility of titanium oxide layer on stainless-steel (SUS316L). The oxide layers with varied thickness were yielded on SUS316L plates by dip-coating of sol-gel solution starting from tetraethyltitanate. The blood compatibility was evaluated in term of platelet adhesion using platelet rich plasma. With increase in the thickness of the oxide layer, the number of adherent platelets decreased rapidly, reached minimum around 150nm. This indicated that the thickness of titanium oxide layer affected platelet adhesion.

scholarly journals Effect of Platelet-rich Plasma Combined With Core Decompression Bone Grafting for Osteonecrosis of Femoral Head: A Meta-Analysis

2021 ◽  
Author(s):  
QiZhong Lai ◽  
Kaishen Cai ◽  
Tianye Lin ◽  
Peng Yang ◽  
Binglang Xiong ◽  
...  
Keyword(s):  
Femoral Head ◽  
Total Hip Arthroplasty ◽  
Bone Grafting ◽  
Platelet Rich Plasma ◽  
Meta Analysis ◽  
Core Decompression ◽  
Osteonecrosis Of Femoral Head ◽  
Group A ◽  
Group B ◽  
Better Than

Abstract Objective: Core decompression bone grafting usually is used hip-preserving approach for osteonecrosis of femoral head (ONFH). Platelet-rich plasma (PRP) is an adjuvant therapy, combined with core decompression bone grafting for ONFH. However, it remains controversial. Therefore, its efficacy was systematically evaluated and meta-analysis in this study.Methods: Literature on core decompression bone grafting for ONFH was retrieved in CNKI, Wan Fang, PubMed, Embase, Cochrane Library and Web of Science from inception to March 2021. Review Manager 5.3 software and Stata 12.0 software were used for data synthesis.Results: A total of 10 RCTs were included. The results showed at final follow-up that, Harris hip score was significantly difference in the treatment group, adjuvant therapy with PRP (group A) better than the control group (group B), MD=7.53 [95%CI (5.29,9.77)]，P < 0.00001. There was MD=-0.71[95%CI (-0.96, -0.46)], P < 0.00001, of visual analog scale (VAS) of hip pain between the two groups. The excellent and good rate of function of hip was 1.42-fold higher in group A than that in group B, RR=1.42, 95%CI (1.25,1.62), P<0.00001. The progression and total hip arthroplasty were showed improvements, RR=0.37,95%CI (0.21,0.65), P=0.0006 and RR=0.39,95%CI (0.18,0.85), P=0.02, respectively. Begg's and Egger's tests did not indicate publication bias.Conclusion: It was shown that the use of PRP combined with core decompression bone grafting improved the symptoms better than core decompression bone grafting only, and might delay progression and total hip arthroplasty. However, as the study’s limitations, it needed to be fully verified by more large-sample multicenter prospective clinical studies.

scholarly journals Ultrafiltration of α-Lactalbumin Protein: Acquaintance of the Filtration Performance by Membrane Structure and Surface Alteration

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3632
Author(s):  
Nasrul Arahman ◽  
Cut Meurah Rosnelly ◽  
Yusni Yusni ◽  
Afrillia Fahrina ◽  
Silmina Silmina ◽  
...  
Keyword(s):  
Membrane Surface ◽  
Pure Water ◽  
Multiple Roles ◽  
Pluronic F127 ◽  
Water Contact ◽  
Surface Alteration ◽  
Pressure Stabilization ◽  
Pes Membrane ◽  
Cell Inhibition ◽  
Better Than

α-Lactalbumin is an essential protein with multiple roles in physiological and the nutritional functionalities, such as diabetic prevention, blood pressure stabilization, and cancer cell inhibition. In the present work, polyethersulfone (PES)-based membranes were developed by incorporating Pluronic F127 and carbon nanotubes with single- and multi-walled dimensions (Sw-Cnts and Mw-Cnts) as additives. The resulting membranes were evaluated for use in the filtration of α-lactalbumin protein solution. Four series of membranes, including PES pristine membrane, were fabricated via the phase inversion process. The characteristics of the membrane samples were analyzed in terms of morphology, membrane surface hydrophilicity and roughness, and surface chemistry. The characterization results show that the incorporation of additive increased the surface wettability by reducing the surface water contact angle from 80.4° to 64.1° by adding F127 and Mw-Cnt additives. The highest pure water permeability of 135 L/(m2·h·bar) was also exhibited by the PES/F127/Mw-Cnt membrane. The performance of the modified membranes was clearly better than the pristine PSF for α-lactalbumin solution filtration. The permeability of α-lactalbumin solution increased from 9.0 L/(m2·h·bar) for the pristine PES membrane to 10.5, 11.0 and 11.5 L/(m2·h·bar) for membranes loaded with Pluronic F127, Sw-Cnts, and Mw-Cnts, respectively. Those increments corresponded to 17, 22, and 28%. Such increments could be achieved without altering the α-lactalbumin rejections of 80%. Remarkably, the rejection for the membrane loaded with Sw-Cnts even increased to 89%.

Multiple Platelet-Rich Plasma Injections Versus Single Platelet-Rich Plasma Injection in Early Osteoarthritis of the Knee: An Experimental Study in a Guinea Pig Model of Early Knee Osteoarthritis

2019 ◽  
Vol 47 (10) ◽  
pp. 2300-2307 ◽  
Author(s):  
Devendra K. Chouhan ◽  
Mandeep S. Dhillon ◽  
Sandeep Patel ◽  
Tungish Bansal ◽  
Alka Bhatia ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Single Injection ◽  
Platelet Rich Plasma ◽  
Early Osteoarthritis ◽  
Short Term ◽  
Multiple Injections ◽  
The Mean ◽  
Guinea Pig Model ◽  
Better Than

Background:Platelet-rich plasma (PRP) has emerged as the forerunner among disease-modifying treatment options for early osteoarthritis (OA) of the knee. However, no consensus is available regarding optimum dosing schedules.Purpose:To determine whether multiple injections of PRP (3 injections) provide better short-term and long-term results than a single injection of PRP in a guinea pig model of knee OA.Study Design:Controlled laboratory study.Methods:36 Dunkin-Hartley guinea pigs (weighing ~600-800 g) were chosen for this study. The animals were assigned to group DC (disease control group), group G1 (single-PRP group), and group G2 (multiple-PRP group) containing 10, 10, and 12 animals, respectively. Another 4 animals were used for preparation of allogenic PRP. Groups G1 and G2 received 1 and 3 injections of PRP, respectively, at weekly intervals in the intervention knee while the contralateral knee was injected with normal saline. Group DC received no intervention in either knee. Half of the animals from each group (subgroups DC.3, G1.3, and G2.3) were sacrificed at 3 months, and the remaining half (subgroups DC.6, G1.6, and G2.6) were sacrificed at 6 months after intervention. Both knee joints were harvested for histological assessment of articular cartilage and synovium.Results:The mean synovial scores for groups G1 and G2 were significantly better than those for group DC at 3 months. No difference was found between groups G1 and G2 at 3 months. At 6 months, group G2 had significantly better mean synovial scores than group G1 and group DC. The mean articular cartilage scores in group G2 were significantly better than those in group DC at 3 months. However, at 6 months, no significant difference was found among any of the groups in terms of mean articular scores.Conclusion:Both single and multiple injections of PRP exert similar anti-inflammatory effects on the synovium in the short term. However, this effect is sustained in the long term only for multiple injections. Multiple injections of PRP exert a chondroprotective effect, but only in the short term. This effect is not seen with a single injection of PRP.Clinical Relevance:This study provides insight into the histological basis for the superiority of multiple injections of PRP.

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