Effect of different dosage of EMS on germination, survivability and morpho-physiological characteristics of sunflower seedling

Helia ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Sheikh Hasna Habib ◽  
Md. Abdul Latif Akanda ◽  
Pryanka Roy ◽  
Hossain Kausar
Keyword(s):  
Large Scale ◽  
Agricultural Research ◽  
Dry Weight ◽  
Research Centre ◽  
Quadratic Regression ◽  
Early Seedling Growth ◽  
Early Seedling ◽  
Sunflower Seedling ◽  
Biology Laboratory ◽  
Leaf Dry Weight

Abstract To find out the effect of EMS dosage on sunflower variety BARI Surjamukhi-2, different EMS concentrations were compared at the molecular biology laboratory (MBL), Oilseed Research Centre (ORC), Bangladesh Agricultural Research Institute (BARI), Gazipur. A total of 10 seeds were treated with each treatment and % germination, % survival and early seedling growth was investigated. Decreased % germination was found with the increasing dose of EMS for all the treatment compared to the control, except for T 4 (0.6% EMS) treatment where a 100% seed was found to be germinated. With an exception of treatment T 4, a decreasing trend of % survivability with the increasing dose of EMS was observed. The gradual reduction with the increasing dose of EMS were observed for most of the studied characters such as leaf area, number of internodes, internode length, fresh and dry weight of leaf, dry weight of shoot, and fresh and dry weight of root in comparison to non-treated control seedlings. However, stimulatory effect with lower dosage of EMS was observed for shoot length in T 2 (0.2% EMS), root length in T 3 (0.4% EMS), and stem fresh weight in T 2 over control. Based on a quadratic regression analysis, using % survivability data, LD50 was calculated and optimum EMS dose was found 0.5%. Hence, the optimized EMS dose of 0.5% may be used to develop large scale desirable mutant of sunflower variety BARI Surjamukhi-2.

Physiological response of chickpea (Cicer arietinum L.) at early seedling stage under salt stress conditions

2019 ◽  
Author(s):  
Anita Mann ◽  
Gurpreet Kaur ◽  
Ashwani Kumar ◽  
Satish Kumar Sanwal ◽  
Jogendra Singh ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salt Tolerance ◽  
Seedling Growth ◽  
Root Length ◽  
Dry Weight ◽  
Shoot Length ◽  
Seedling Stage ◽  
Tolerance Index ◽  
Early Seedling Growth ◽  
Early Seedling

Screening of chickpea lines for salt tolerance through seed germination and early seedling growth is crucial for their evaluation. Seeds of 30 chickpea genotypes were germinated on a sand bed irrigated with saline (3, 6, 9, 12 dS/m) and control solutions upto 30 days. At the early seedling stage (25-30 days), germination percentage, chlorophyll content, proline, root length, shoot length and seedling dry weight were found to be affected due to salinity. Salt tolerance index (STI) for plant biomass maintained a significant correlation with chlorophyll, proline, shoot length, and root length, which indicated that these parameters could be used as selection criteria for screening chickpea genotypes against salt stress. Significant differences in shoot length, root length, and seedling dry weight in 30-day-old seedlings were observed among selected chickpea genotypes as well. From the overall observation of germination characterstics and early seedling growth, it is concluded that the chickpea genotypes, HC-1, HC-5, ICC 867, ICC 5003, H-10-41 showed better salt tolerance as compared to the available salt tolerant check variety.

scholarly journals Interaction of SiO2 Nanoparticles with Seed Prechilling on Germination and Early Seedling Growth of Tall Wheatgrass (Agropyron Elongatum L.)

2014 ◽  
Vol 16 (3) ◽  
pp. 25-29 ◽  
Author(s):  
Reyhane Azimi ◽  
Mohammad Jankju Borzelabad ◽  
Hassan Feizi ◽  
Amin Azimi
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Dry Weight ◽  
Sio2 Nanoparticles ◽  
Control Group ◽  
Agropyron Elongatum ◽  
Control Seed ◽  
Tall Wheatgrass ◽  
Early Seedling Growth ◽  
Early Seedling

Abstract The effect of six SiO2 nanosized concentrations (0, 5, 20, 40, 60 and 80 mg L-1) and three seed prechilling treatments (control, seed prechilling before nano SiO2 treatments, treatments of seed with nano SiO2 before prechilling) on germination and seedling growth of tall wheatgrass (Agropyron elongatum L.) were studied. Results indicated that application of SiO2 nanoparticles significantly increased seed germination of tall wheatgrass from 58 percent in control group to 86.3 and 85.7 percent in 40 and 60 mg L-1, respectively. Applying SiO2 nanoparticles increased dry weight of shoot, root and seedling of tall wheatgrass. Increasing concentration of nanoparticle from 0 up to 40 mg L-1 increased seedling weight around 49 percent compared to the control, nevertheless decreased under 60 and 80 mg L-1 treatments. In conclusion, seed prechilling in combination with SiO2 nanoparticles largely broke the seed dormancy for A. elongatum.

scholarly journals Seed Priming Significantly Affects Germination and Post Germination Early Seedling Growth in Okra

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Rima Rima
Keyword(s):  
Root Length ◽  
Dry Weight ◽  
Seed Priming ◽  
Germination Percentage ◽  
Shoot Length ◽  
Seedling Height ◽  
Seedling Vigor ◽  
Commercial Cultivation ◽  
Early Seedling Growth ◽  
Early Seedling

AbstractSeed priming significantly affects germination and post germination emergence in seedling of plants. Okra is an important vegetable plays significant role in human nutrition. However, little information is available regarding effect of seed priming on germination and post germination early vegetative growth. Therefore, a pot experiment was conducted to find out the effect of seed priming on the emergence and seedling vigor in different okra varieties. Six local Bangladeshi okra varieties were primed in water for 24 hours and evaluated their germination and post germination emergence. The varieties were named as V1-V6 (V1 = Flash power, V2 = Durga, V3 = BARI dheros-2, V4 =Okra pornota, V5 =Dandy all green, V6 = Boishakhi). Post priming evaluation was done based on germination percentage, root-shoot length of seedlings, seedling height, and dry weight of seedlings, germination index and speed of germination at different days after sowing (5-15 DAS). Highest germination was observed in V3 (88.00%) and lowest was in V5 (25.33%). Root length was highest in V3 and V4 (8.77cm) and lowest was observed in V2 (5.15cm). Shoot length of the seedlings was highest in V3 (7.94cm) followed by V1 (7.33). Shoot length of the seedlings was lowest in V5 (5.12cm). Seedling height was highest in V3 (16.71cm) which was followed by V6 (16.26cm). The lowest seedling height was observed in V1 (12.50cm). Dry weight of the seedlings was highest in V3 (2.32g) which was followed in V1 (2.26g). The lowest dry weight was observed in V5 (1.99g). The variety V3 performed better in most of the evaluated parameters and suggested for commercial cultivation.

scholarly journals Effect of acid stress on germination and early seedling growth of red clover

2011 ◽  
Vol 27 (3) ◽  
pp. 1295-1303 ◽  
Author(s):  
V. Mandic ◽  
Z. Tomic ◽  
V. Krnjaja ◽  
Z. Bijelic ◽  
M. Zujovic ◽  
...  
Keyword(s):  
Acid Stress ◽  
Red Clover ◽  
Animal Husbandry ◽  
Dry Weight ◽  
Fresh Weight ◽  
Hard Seed ◽  
Early Seedling Growth ◽  
Vigor Index ◽  
Seedling Length ◽  
Early Seedling

The aim of this investigation was to estimate the effect of four pH levels of germination media (4, 5, 6 and 7) on seed germination energy (GE), germination (G), percentage of dead or infected seeds (DIS), percentage of hard seed (HS), normal (NS) and abnormal seedlings (AS), root length (RL), shoot length (ShL), seedling length (SeL), fresh weight (FW) and dry weight (DW) of seedling, and seedling vigor index (SVI) in two red clover genotypes (cv. K-17 and cv. Kolubara). The experiment was conducted in the laboratory conditions of the Institute for Animal Husbandry in Belgrade in January 2011. The pH levels of germination media had significant effect on the NS (P<0.01), AS (P<0.01), RL (P<0.05), ShL (P<0.01), SeL (P<0.01), FW (P<0.01) and SVI (P<0.05). The lowest GE, G, NS, ShL, SeL, FW and SVI were found at pH 4. Also, the highest DIS, HS and AS were found at pH 4. The genotype had significant effect on HS (P<0.01), RL (P<0.01), ShL (P<0.01), SeL (P<0.01), FW (P<0.01) and SVI (P<0.01). Genotype and pH levels of germination media did not affect on GE, G, DIS and DW significantly.

scholarly journals Genetic variability of red clover seedlings in relation to salt stress

2014 ◽  
Vol 30 (3) ◽  
pp. 529-538 ◽  
Author(s):  
V. Mandic ◽  
V. Krnjaja ◽  
Z. Bijelic ◽  
Z. Tomic ◽  
A. Simic ◽  
...  
Keyword(s):  
Salt Stress ◽  
Seedling Growth ◽  
Red Clover ◽  
Dry Weight ◽  
Growth Stages ◽  
Early Seedling Growth ◽  
Vigor Index ◽  
Growing Medium ◽  
Early Seedling ◽  
Sterile Plastic

Red clover is highly salt-sensitive plant, especially during germination and early seedling growth stages. The aim of this investigation was to estimate the effects of different saline conditions (0, 50, 100, 150, and 200mM NaCl) on germination and early seedling growth in four red clover varieties (Kolubara, K-32, K-17 and K-39). Germination test was conducted in the laboratory conditions using sterile plastic vessels on filter paper moistened with 10ml of the appropriate salt test solutions. It was observed that the germination energy (GE), germination (G), percentage of dead or infected seeds (DIS), normal seedlings (NS), root length (RL), shoot length (ShL), fresh weight (FW) and dry weight of seedling (DW) and seedling vigor index (SVI) were significantly decreased with increasing concentrations of NaCl in the growing medium. The tested varieties of red clover showed different NaCl tolerance at the seedling stage. Generally, studied red clover varieties are very sensitive to salt, especially K-32 which has the lowest values for GE, G, NS and SVI and highest for DIS. Variety K-17 proved to be a variety that the best tolerates conditions of salt stress because the values for GE, G, NS, RL and SVI were highest. Testing of varieties of red clover in the early seedling growth at different concentrations of NaCl in the growing medium could be helpful in the identification and selection of varieties for cultivation on saline soils.

Effects of temperature and salinity on the germination of Puccinellia ciliata (Bor) cv. Menemen

1989 ◽  
Vol 40 (3) ◽  
pp. 561 ◽  
Author(s):  
BA Myers ◽  
DI Couper
Keyword(s):  
Dry Weight ◽  
Germination Percentage ◽  
Distilled Water ◽  
Salt Tolerant ◽  
Night Temperature ◽  
Osmotic Effect ◽  
Shoot Dry Weight ◽  
Early Seedling Growth ◽  
Early Seedling ◽  
Effects Of Temperature

Puccinecia ciliata is a salt-tolerant, winter-growing grass. The responses of germination of P. ciliata to salinity and temperature were investigated. With a day/night temperature regime of 25/15�C, the final germination percentage of P. ciliata in distilled water was 54%, and was not significantly affected by salinities up to 87 mol m NaCl (osmotic potential, =, -0.4 MPa), but decreased to 5.2% in 260 mol m-3 NaCl (=, -1.2 MPa). The n at which the final germination percentage was reduced by 5O0/n of that in distilled water was about -0.5 MPa, which was similar to that of Lolium perenne. However, for L. perenne, the final germination percentage decreased more rapidly with increasing salinity and from a higher value in distilled water (97%) compared with P. ciliata. Germination of P. ciliata was completely inhibited at constant 33�C and was low (<20%) where day temperature was 30�C and night temperature was greater than or equal to 17�C. Maximum germination was observed where day and night temperatures were between 17 and 24�C, but were not equal. The rate of germination decreased with increasing salinity and was maximal at temperatures between 17�C and 24�C. The reduction in the final germination percentage in NaCl solution was apparently an osmotic effect since the final germination percentage in iso-osmotic solutions of mannitol, polyethylene glycol, NaCl, KC1 and KNO3 were similar. P. ciliala and L. perenne were more salt sensitive during germination than during early seedling growth; for 35-day-old seedlings, shoot dry weight was reduced by 50% of that of the nonsaline treatment when irrigated with solution with = of -0.75 MPa for L. perenne and - 1.25 MPa for P. ciliala.

scholarly journals Effect of irrigation levels at different growth stages on growth parameters and yield of four selected chilli accessions

1970 ◽  
Vol 34 (1) ◽  
pp. 143-155 ◽  
Author(s):  
MAI Khan ◽  
AM Farooque ◽  
MA Hoque ◽  
MA Rahim ◽  
MA Haque
Keyword(s):  
Agricultural Research ◽  
Growth Parameters ◽  
Dry Weight ◽  
Fruit Weight ◽  
Water Levels ◽  
Research Centre ◽  
Growth Stages ◽  
Plant Leaf ◽  
Water Treatments ◽  
Different Growth Stages

An experiment as conducted at Bangladesh Agricultural University. Mymensingh in poly bags under glasshouse condition during October 1997 to March 1998 to know the effects of different water levels at different growth stages of four selected chilli accessions viz., C-027l, C-0272, C-0275, and C-0277. Six water treatments viz., watering once everyday (W1), watering twice everyday (W2). watering at 4 days interval (W3), watering at 8 days interval (W4), watering at 16 days interval (W5), and no watering (W0) were tested at three growth stages viz., Vegetable stage (S1), Flowering stage (S2), and Fruiting stage (S3). The accessions of chilli were selected from the results of a previous experiment where ten accessions of chilli collected from Spices Research Centre (SRC) of Bangladesh Agricultural Research Institute, Joydebpur, Gazipur were used. The primary selection was made on the basis of their tolerance and susceptibility to water stages. The results revealed that all the studied parameters viz., plant height, canopy diameter, root length, root volume, no. of fruits per plant, fruit length, fruit diameter, individual fruit weight, no. of leaves per plant, leaf area per plant, fruit yield per plant, leaf dry weight, stem dry weight, root dry weight, fruit dry weight per plant, varied significantly among the accessions under different water treatments at different stages of growth. Out of four accessions, C-0271 and C-0277 were found as water stress tolerant and susceptible, respectively. Key Words: Water level; growth stage; chilli accessions DOI: 10.3329/bjar.v34i1.5764Bangladesh J. Agril. Res. 34(1) : 143-155, March 2009

scholarly journals Effect of waste water treated with TiO2 nanoparticles on early seedling growth of Zea mays L.

2017 ◽  
Vol 8 (3) ◽  
pp. 424-431 ◽  
Author(s):  
Sonila Yaqoob ◽  
Faizan Ullah ◽  
Sultan Mehmood ◽  
Tariq Mahmood ◽  
Mohib Ullah ◽  
...  
Keyword(s):  
Waste Water ◽  
Seedling Growth ◽  
Dry Weight ◽  
Fresh Weight ◽  
Nano Tio2 ◽  
Shoot Dry Weight ◽  
Root Area ◽  
Early Seedling Growth ◽  
Early Seedling

Abstract The present investigation was to determine the effect of nano-TiO2 (2 to 6 nm) and waste water on early seedling growth of maize. The suspensions of nano-TiO2 either in deionized water or autoclaved waste water were applied at 100 mg/L, 50 mg/L and 25 mg/L under in vitro conditions. Analyses of waste water showed that it was not suitable for irrigation purposes as it had a higher content of heavy metals (Fe, Mn, Zn, Cd, Cr and Cu) which were above permissible levels for irrigation. The higher concentration of nano-TiO2 (100 mg/L) and waste water significantly inhibited seed germination, seedling growth and caused accumulation of phenolics in maize plants (p &lt; 0.05). The application of nano-TiO2 at 25 mg/L significantly increased shoot fresh weight, shoot dry weight, root fresh weight, root dry weight, root area, chlorophyll a, chlorophyll b and carotenoids content (p &lt; 0.05). The adverse effects of waste water on growth attributes of maize were significantly ameliorated by nano-TiO2 at 25 mg/L (p &lt; 0.05). The treatment of waste water with nano-TiO2 (25 mg/L) is recommended before its utilization for agriculture purposes.

Presowing Hydration-Dehydration Treatments in Relation to Seed Germination and Early Seedling Growth of Wheat and Ryegrass

1981 ◽  
Vol 8 (5) ◽  
pp. 409 ◽  
Author(s):  
WM Lush ◽  
RH Groves ◽  
PE Kaye
Keyword(s):  
Seedling Growth ◽  
Dry Weight ◽  
High Water ◽  
Lag Phase ◽  
Annual Ryegrass ◽  
Early Seedling Growth ◽  
Threshold Temperatures ◽  
Early Seedling ◽  
A Minor ◽  
Water Contents

Seeds of wheat (Triticum aestivum cv. Egret) and annual ryegrass (Lolium rigidum cv. Wimmera) were given a single hydration-dehydration cycle by adding a known amount of water and later air-drying them. Embryos enlarged during this initial hydration, and short periods at high water contents were equivalent to longer periods at lower water contents. After hydration to 145% of seed dry weight (wheat) or to 155% (ryegrass) followed by dehydration back to the original seed weights the speed of germination of both species was approximately doubled because the lag phase of germination was shortened. The proportion of ryegrass seeds that were dark-dormant was reduced if seeds were pretreated in light. Pretreatment did not increase germination synchrony, alter threshold temperatures for germination or enhance seedling growth rates. The viability of pretreated seed declined rapidly at - 15°C and at 50°C. The effect of pretreatment, i.e. early germination, is attributed mainly to embryo enlargement. More rapid imbibition of pretreated seeds may contribute in a minor way to differences in germination time.

scholarly journals The influence of genotype and osmotic stress on germination and seedling of maize

2014 ◽  
Vol 30 (2) ◽  
pp. 357-366 ◽  
Author(s):  
V. Mandic ◽  
Z. Bijelic ◽  
V. Krnjaja ◽  
Z. Tomic ◽  
A. Simic ◽  
...  
Keyword(s):  
Osmotic Stress ◽  
Dry Matter ◽  
Dry Weight ◽  
Tolerance Index ◽  
Fresh Weight ◽  
Shoot Dry Weight ◽  
Early Seedling Growth ◽  
Vigor Index ◽  
Early Seedling ◽  
Sterile Plastic

The aim of this research was to estimate the influence of different NaCl osmotic solutions (0, -0.3 MPa, -0.6 MPa, -0.9 MPa, -1.2 MPa, -1.5 MPa) on seed germination, and early seedling growth in two maize hybrid different maturity groups (ZP 560 - FAO 500 and ZP 666 - FAO 600). Germination was tested in sterile plastic vessels on filter paper moistened with different NaCl solutions, in the dark at 20 ? 1?C, in laboratory. Results of ANOVA indicated that hybrid ZP 666 had significantly higher root length (RL) (6.37 cm), shoot length (ShL) (2.06 cm), shoot fresh weight (ShFW) (43.86 mg), root dry weight (RDW) (7.56 mg), shoot dry weight (ShDW) (5.97 mg), seedling vigor index (SVI) (706.55) and dry matter stress tolerance index (DMSI) (55.85%) than hybrid ZP 560 (4.18 cm, 1.14 cm, 32.50 mg, 6.54 mg, 4.15 mg, 457.61 and 48.86%, respectively). Contrary, hybrid ZP 560 had significantly higher relative seedling water content (RSWC) (83.83%) and phytotoxicity of shoot (PhSh) (69.77%) than hybrid ZP 666 (81.16% and 62.51%, respectively). Generally, hybrid ZP 666 had better tolerance to salt stress than hybrid ZP 560. Germination energy (GE), germination (G), RL, ShL, root fresh weight (RFW), ShFW, RDW, ShDW, rate germination index (RGI), SVI, RSWC and DMSI were significantly decreased with the increase in osmotic stress induced by NaCl. Contrary, phytotoxicity of root (PhR) and PhSh significantly increased with the increase in osmotic stress.

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