scholarly journals Evaluation of the virulence of Sclerotium rolfsii isolates on Arachis hypogaea and screening for resistant genotypes in greenhouse conditions

2015 ◽  
Vol 8 (1) ◽  
pp. 1-11 ◽  
Author(s):  
A.A. Eslami ◽  
S.A. Khodaparast ◽  
S. Mousanejad ◽  
F. Padasht Dehkaei
Keyword(s):  
Plant Height ◽  
Block Design ◽  
Sclerotium Rolfsii ◽  
Soil Surface ◽  
Lesion Length ◽  
Randomized Block Design ◽  
Wide Range ◽  
Mycelial Compatibility ◽  
Pathogenicity Tests ◽  
Plant Maturity

Summary Sclerotium rolfsii is a soil borne pathogen responsible for root and stem rot on a wide range of crops. This study was conducted to identify the virulence of different S. rolfsii isolates on a susceptible local peanut germplasm and determine the resistance of 20 peanut genotypes to the most virulent isolate and also the relationship between virulence and mycelial compatibility groups (MCGs). Seventy eight isolates of this fungus from 10 host plants and six known MCGs were used in the experiment. The experiment was done in greenhouse conditions (25±5°C) using a complete randomized block design with three replications. Pots containing sterile soil (pH=6.7) were inoculated with barley seeds colonized by each isolate separately before being seeded with the peanut germplasm. Disease severity was assessed by scoring the wilting, yellowing or death of plants, mycelia or sclerotia production on the soil surface or on plant stem, stem area affected (%) and stem lesion length, at the stage of plant maturity. Also, shoot wet weight and plant height were recorded at this stage. According to the results of the pathogenicity tests, all of the isolates were virulent on the susceptible peanut germplasm and the virulence diff ered signifi cantly between the isolates (P≤0.01). There was no relationship between the virulence of the five groups of isolates identified in the present study and the MCGs. The peanut genotype 140, which was better than the others based on seed size, plant height and the canopy size, was also the most resistant one

scholarly journals Penggunaan Biochar dan Tinggi Muka Air pada Umur Satu Bulan setelah Tanam terhadap Pertumbuhan dan Hasil Tanaman Bawang Merah

2019 ◽  
Vol 8 (2) ◽  
pp. 202-212
Author(s):  
Susilawati Susilawati ◽  
Irmawati Irmawati ◽  
Sri Sukarmi ◽  
Astuti Kurnianingsih ◽  
Ade Mutia
Keyword(s):  
Plant Height ◽  
Water Table ◽  
Block Design ◽  
Soil Surface ◽  
Dry Weight ◽  
Growth And Yield ◽  
Leaf Number ◽  
Fresh Weight ◽  
Randomized Block Design ◽  
Basin Scale

Susilawati S, Irmawati I, Sukarmi S, Kurnianingsih A, Mutia A. 2019. The application of biochar and water table at one month after planting on growth and yield of shallot. Jurnal Lahan Suboptimal: Journal of Suboptimal Lands. 8(2):202-212. The success of onion self-sufficiency is the arrangement and growth of production centers that are not only concentrated in Java. The research aimed to evaluate the effect of biochar application and water table treatment on growth and yield of shallot plant. The research was conducted in Experimental Farm of Agriculture Faculty, Sriwijaya University at 2019. Materials used consisted of shallot bulb of Bima Brebes variety, oil palm shells biochar, paper bag, and plastic bag. While the tools used were hoe, gauge, pH meter, basin, scale, and oven. Factorial Randomized Block Design was used with two factors and three replicates. The first factor was biochar dosages consisting of P1 (51 g/plant) and P2 (102 g/plant) and the second factor was soil water table consisting of T0 (no water table treatment), T1 (10 cm of water table below soil surface), T2 (15 cm of water table below soil surface) and T3 (30 cm of water table below soil surface). The observed parameters included plant height, leaf number, tiller number, total bulb fresh weight, bulb fresh weight, bulb dry weight and root length. Results showed that the application of biochar and water table treatment statistically affected the growth and yield of shallot plant. The effect on both growth and yield parameters were obtained from the combination of P2T3. Correlation was found between plant height and leaf number, total bulb fresh weight and bulb fresh weight.

PENGARUH KOMPOS SAMPAH KOTA DAN PUPUK KANDANG SAPI TERHADAP PERTUMBUHAN DAN HASIL TANAMAN PAKCOY (BRASSICA RAPA, L. CHINENSIS) PADA ALUVIAL KABUPATEN INDRAMAYU

2018 ◽  
Vol 9 (2) ◽  
pp. 153-163
Author(s):  
Wiwik Ambarsari ◽  
Henly Yuliana
Keyword(s):  
Plant Height ◽  
Brassica Rapa ◽  
Block Design ◽  
Cattle Manure ◽  
Growth And Yield ◽  
Randomized Block Design ◽  
Two Factors ◽  
Number Of Leaves ◽  
Experimental Garden

The study aimed to determine the effect of the combination of city waste compost and cattle manure on the growth and yield of pakcoy plants (Brassica rapa, L. Chinensis) in Aluvial, Indramayu Regency. The study was conducted at the Experimental Garden of the Faculty of Agriculture, Wiralodra University, Indramayu Regency from March to August 2018. This study used Factorial Pattern Randomized Block Design with two factors. The first factor is city waste compost and the second factor is cattle manure with 4 levels: 0%, 3.0%, 6.0%, and 9.0%, repeated twice. The results showed that there was an independent influence between city waste compost and cattle manure on plant height and number of leaves. The best combination to increase the feasibility weight of pakcoy consumption is by giving city waste compost 9.0% (S4) and cattle manure 6.0% (K3).

scholarly journals Sequence Variation in Two Protein-Coding Genes Correlates with Mycelial Compatibility Groupings in Sclerotium rolfsii

2013 ◽  
Vol 103 (5) ◽  
pp. 479-487 ◽  
Author(s):  
Efrén Remesal ◽  
Blanca B. Landa ◽  
María del Mar Jiménez-Gasco ◽  
Juan A. Navas-Cortés
Keyword(s):  
Rna Polymerase Ii ◽  
Sequence Variation ◽  
Geographic Origin ◽  
Sclerotium Rolfsii ◽  
Nuclear Ribosomal Dna ◽  
Causal Organism ◽  
Protein Coding ◽  
Protein Coding Genes ◽  
Wide Range ◽  
Mycelial Compatibility

Populations of Sclerotium rolfsii, the causal organism of Sclerotium root-rot on a wide range of hosts, can be placed into mycelial compatibility groups (MCGs). In this study, we evaluated three different molecular approaches to unequivocally identify each of 12 previously identified MCGs. These included restriction fragment length polymorphism (RFLP) patterns of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (rDNA) and sequence analysis of two protein-coding genes: translation elongation factor 1α (EF1α) and RNA polymerase II subunit two (RPB2). A collection of 238 single-sclerotial isolates representing 12 MCGs of S. rolfsii were obtained from diseased sugar beet plants from Chile, Italy, Portugal, and Spain. ITS-RFLP analysis using four restriction enzymes (AluI, HpaII, RsaI, and MboI) displayed a low degree of variability among MCGs. Only three different restriction profiles were identified among S. rolfsii isolates, with no correlation to MCG or to geographic origin. Based on nucleotide polymorphisms, the RPB2 gene was more variable among MCGs compared with the EF1α gene. Thus, 10 of 12 MCGs could be characterized utilizing the RPB2 region only, while the EF1α region resolved 7 MCGs. However, the analysis of combined partial sequences of EF1α and RPB2 genes allowed discrimination among each of the 12 MCGs. All isolates belonging to the same MCG showed identical nucleotide sequences that differed by at least in one nucleotide from a different MCG. The consistency of our results to identify the MCG of a given S. rolfsii isolate using the combined sequences of EF1α and RPB2 genes was confirmed using blind trials. Our study demonstrates that sequence variation in the protein-coding genes EF1α and RPB2 may be exploited as a diagnostic tool for MCG typing in S. rolfsii as well as to identify previously undescribed MCGs.

scholarly journals Trace Metal Distribution and Mobility in Soils after Silvicultural Thinning and Burning

2017 ◽  
Vol 9 (5) ◽  
pp. 83
Author(s):  
Ngowari Jaja ◽  
Monday Mbila ◽  
Yong Wang
Keyword(s):  
Trace Metal ◽  
Management Practices ◽  
Soil Depth ◽  
Anthropogenic Activities ◽  
Block Design ◽  
Forest Health ◽  
Soil Surface ◽  
Parent Material ◽  
Design Experiment ◽  
Randomized Block Design

Silvicultural thinning and burning are common management practices that are widely used to address ecosystem problems such as tree stocking and general forest health. However, high-severity fire has variable effects on soils, resulting in damages which are directly or indirectly reflected on the trace metal chemistry of the soil. This study was conducted to evaluate the trace metal variation at the Bankhead National Forest in Northern Alabama following the silvicultural thinning and burning. The experimental site had treatments consisting of two burning patterns and three levels of thinning as part of an overall treatment of three burning patterns and three levels of thinning applied to nine treatment plots to fit a completely randomized block design experiment. Four treatments sites were used for this study and samples were collected from soil profile pits excavated at representative plots within each treatment. The samples were analyzed for trace metals-As, Cu, Ni, Zn and Pb-using Perkin Elmer 2100 ICP-OES. Post treatment samples indicated that the trace metal concentrations generally decreased with soil depth. Copper, Ni, and Zn at the Pre-burn site gradually increased with depth to a maximum concentration at about 50 cm below the soil surface. Arsenic in the surface horizons increased by 156% in the burn-only sites, 54% in the thin-only treatment, 30% for the burn and thin treatments. Such differences were unlikely due to differences in the geochemistry of the parent material, but likely due to anthropogenic activities and possibly the forest management practices in question.

scholarly journals Influence of vernalization and bulb size on the production of lily cut flowers and lily bulbs

2017 ◽  
Vol 38 (4Supl1) ◽  
pp. 2399 ◽  
Author(s):  
Diogo Brito de Almeida ◽  
José Geraldo Barbosa ◽  
José Antônio Saraiva Grossi ◽  
Fernando Luiz Finger ◽  
Joice Crescencio Heidemann
Keyword(s):  
Plant Height ◽  
Block Design ◽  
Cut Flowers ◽  
Floral Buds ◽  
Randomized Block Design ◽  
Commercial Harvest ◽  
Physiological Phenomenon ◽  
Cold Chamber ◽  
The Impact

Exposure of bulbs to cold, a physiological phenomenon called vernalization, and bulb size are important factors in the production of lily bulbs and flowers. This study aimed to verify the influence of vernalization of bulbs on flowering cut lily plants, as well as the impact of size and shape of harvest on the production and quality of flowers and bulbs. In turn, the way the stems of the plants used for cut-flower production are cropped is of higher importance for the production of new flower bulbs. In this sense, the experiment was conducted in Viçosa, MG, in a greenhouse in a randomized block design, in split splot scheme with three replications, in which the vernalization periods (25, 35 and 45 days at 4 ± 1 C) constituted the plots; bulb sizes (diameters of 3.2-3.8 cm; 2.5-3.2 cm 1.9- and 2.5 cm), subplots and ways to harvest (full harvest of the stem at the required length for the commercial harvest of the flower, commercial stem harvest at the commercial length, maintaining 10cm of stem in the soil; removal of the floral buds as soon as their appearance is observed and harvest at the end of the season), the sub subplots. The bulbs were planted in beds, with 15 x 20 cm spacing. It was evaluated the number of plants that flowered and the number of flowers, the length and the diameter of the floral buds, fresh and dry weights, diameter and plant height as well as number, perimeter and amount of fresh and dry bulbs. There was a decrease in the plant height with the increase of the vernalization period and a reduction of the diameter of the planted bulbs, as well as of the number and the fresh and dry weights of the produced buds. The production of flowers and buds in number, size and weight was directly proportional to the size of the planted bulbs, while the form of harvest with removal of flower buds increased the number, the perimeter and the fresh and dry weights of the buds. Bulbs with diameter between 3.2 - 3.8 cm, stored for 25 days in cold chamber enable greater production of flowers and the highest stem height, factors that favor the increase in market value.

scholarly journals Yield and vegetative growth of cactus pear at different spacings and under chemical fertilizations

2016 ◽  
Vol 20 (6) ◽  
pp. 564-569 ◽  
Author(s):  
João A. da Silva ◽  
Sérgio L. R. Donato ◽  
Paulo E. R. Donato ◽  
Evilasio dos S. Souza ◽  
Milton C. Padilha Júnior ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Vegetative Growth ◽  
Dry Matter ◽  
Block Design ◽  
Plant Age ◽  
Cactus Pear ◽  
Area Index ◽  
Randomized Block Design

ABSTRACT The objective was to evaluate the effect of different spacings and mineral fertilizations on cactus pear growth and production in a randomized block design, with three replicates, in a 3 x 4 factorial scheme: three spacings, 1.00 x 0.50 m, 2.00 x 0.25 m and 3.00 x 1.00 x 0.25 m, and four fertilizations, 000-000-000, 000-150-000, 200-150-000 and 200-150-100 kg ha-1 of N, P2O5 and K2O, respectively. Plant growth was evaluated between 90 and 390 days and production and growth were evaluated at 620 days after planting. There were significant interactions between spacing and fertilization for plant height, number of cladodes and cladode area index from 90 to 390 days and for production of fresh and dry matter at 620 days after planting. Spacing influenced cladode area index, while fertilization influenced plant height, number of cladodes and cladode area index at 620 days after planting. Plant height showed cubic effect for the days after planting. Number of cladodes and cladode area index were dependent on spacing, fertilization and plant age, and fitted to cubic models. The best results of growth and production of fresh and dry matter are associated with NPK and NP fertilizations and the spacing of 1.00 x 0.50 m.

scholarly journals Pertumbuhan dan Produksi Dioscorea sansibarensis Pax pada Perlakuan Panjang lanjaran dan Waktu Batang Membelit*

2018 ◽  
Vol 45 (3) ◽  
pp. 249
Author(s):  
Solikin ,
Keyword(s):  
Block Design ◽  
Soil Surface ◽  
Dry Weight ◽  
Growth And Yield ◽  
Botanic Garden ◽  
Randomized Block Design ◽  
Two Factors ◽  
Main Plot ◽  
Total Plant ◽  
Total Dry Weight

Dioscorea is potentially used as staple food to support food security. The research was aimed to determine the effect of stake length and time of  stem twining  on  the growth of Dioscorea sansibarensis Pax. The experiment was conducted  in  Purwodadi Botanic Garden from December 2014 until May 2015 using split plot randomized block design consisted of two factors, i.e. the stake length and time of stem twining. The stake length was the main plot consisted of 150 cm, 100 cm and 50 cm above soil surface and without stake (control). The time of stem twining as subplots, i.e. stem twined early, twined at the time of 4 leaves stage, and twined at 8 leaves stage. Each combination of the treatments was replicated three times. The results showed that there was significant effect on the stake length  and the time of stem twining treatments on the plant growth and yield. The stake  length of 150 cm treatment produced the highest fresh tuber and total plant dry weight, i.e., 257.24 g and 132.77 g per plant, respectively. On the contrary, the plant without stake produced the lowest fresh tuber and  total dry weight of plant, i.e., 112.10 g and 48.65 g per plant, respectively.<br /><br />Keywords: biomass,leaf area, photosyntesis, tuber weight

scholarly journals Response of growth and yield of two soybean varieties to seed pre-treatment applications

2021 ◽  
Vol 883 (1) ◽  
pp. 012052
Author(s):  
N Herawati ◽  
A R Aisah ◽  
I Mardian ◽  
B N Hidayah ◽  
B T R Erawati
Keyword(s):  
Plant Height ◽  
Seed Treatment ◽  
Seed Quality ◽  
Block Design ◽  
Growth And Yield ◽  
Agronomic Crops ◽  
Randomized Block Design ◽  
Two Factors ◽  
Pre Treatment ◽  
Four Levels

Abstract Plant growth is influenced by seed quality. Seeds need to be treated to prevent pest and disease disorders or to increase seed germination. Soybean planting was carried out in this study by the treatment of varieties and seed pre-treatment application before planting. The study aimed to measure the growth and yield of soybeans by varieties and seed treatment before planting, carried out in the Village of Nggembe, District of Bolo, Bima Regency. The study used factorial randomized block design with two factors. The first factor was soybean varieties consisting of two levels, namely Devon and Dena varieties, and the second factor was the application of pre-planting seed treatments consisting of four levels namely Cruiser, Marshall, Agrisoy, and without seed treatment, each treatment was repeated three times. Observations were made on the performance of agronomic crops and soybean yields. The results showed that the treatment of soybean varieties and the application of seed treatment had a significant influence on plant height and soybean productivity, and both treatments had interactions on parameters of plant height, number of filled pods, weight of 100 seeds, and soybean productivity with the best results successively produced by a combination of Devon-Control, Devon-Marshall, Devon-Agrisoy, and Dena-Cruiser.

scholarly journals Genetic Analysis of Some Genotypes of Indian Mustard (Brassica juncea L.) for Yield and Yield Attributing Traits

2021 ◽  
pp. 51-60
Author(s):  
A. M. Pradhan ◽  
M. Roy Choudhury ◽  
A. Sawarkar ◽  
S. Das
Keyword(s):  
Plant Height ◽  
Seed Yield ◽  
Seed Weight ◽  
Block Design ◽  
Indian Mustard ◽  
Correlation Study ◽  
Biotic And Abiotic Stress ◽  
Randomized Block Design ◽  
Additive Gene ◽  
Positive Direct

The current investigation was carried out to examine the selection criteria for yield improvement in selected genotypes of Indian mustard. Twenty-four genotypes of Indian mustard were evaluated for seed yield and yield attributing characters with randomized block design with three replications. The analysis of variance showed significant differences among all the 24 genotypes against all the characters. The phenotypic and genotypic coefficient of variation were higher for most of the traits like seed yield per plant, number of secondary branches per plant and number of siliquae per plant. High heritability coupled with high genetic advance in percent of mean was registered for number of siliquae per plant, number of primary and secondary branches per plant, plant height, 1000 seed weight, siliqua length per plant and seed yield per plant suggesting predominant role of additive gene action for expression of these traits. The correlation study revealed that seed yield per plant was positively and significantly correlated with number of primary and secondary branches per plant, number of siliquae per plant, siliqua length per plant and 1000 seed weight at both phenotypic and genotypic level. The traits namely, number of secondary branches per plant recorded as highest positive direct effect followed by number of seeds per siliqua, number of siliquae per plant and plant height. Therefore, they may be carried out further evaluation in multilocational trials, biotic and abiotic stress like environmental conditions to check their stability and adaptability.

scholarly journals Optimasi Dosis Pupuk Kandang Ayam dan NPK pada Budidaya Kubis Bunga (Brassica oleracea var. Botrytis L.)

2021 ◽  
Vol 2 (1) ◽  
pp. 32
Author(s):  
Irpan Gunawan ◽  
Atak Tauhid ◽  
Isna Tustiyani
Keyword(s):  
Leaf Area ◽  
Plant Height ◽  
Block Design ◽  
Chicken Manure ◽  
Growth And Yield ◽  
Randomized Block Design ◽  
Affected Plant ◽  
Two Factors ◽  
Number Of Leaves ◽  
Npk Fertilizer

<p><em>Cauliflower is one of the vegetables for consumers. The demand for cauliflower was rising so it must be scaled up with fertilizer. The purpose of this study was to study the effect of chicken manure and NPK fertilizer on the growth and yield of cauliflower. The study was conducted in Sukasenang Village, Banyuresmi Sub-district, Garut Regency from July to August 2019. The study used a Randomized Block Design (RBD) in two factors each of the 3 rates with 2 replications. The first factor was the rates of chicken manure which consisted of 0, 10 and 20 tons ha<sup>-1</sup>; the second factor was NPK fertilizer which consists of 0, 100 and 200 kg ha<sup>-1</sup>. The parameter of this research was plant height, number of leaves, leaf area, weight and diameter crud. The results showed that there was no interaction between the chicken manure and NPK fertilizer. The treatment of 20 tons ha<sup>-1</sup> chicken manure affected the variable plant height, the number of leaves and leaf area. The rates of 200 kg ha<sup>-1</sup> NPK fertilizer had affected plant height, number of leaves, weight and diameter crud.</em></p>

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